Standard name
Human Ortholog
Description Possible shared subunit of Cop9 signalosome (CSN) and eIF3; binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.98 0.97 0.96 0.94 0.78 0.68 0.8 0.67 0.66 0.58 0.62 0.99 0.99 0.99 0.85 0.88 0.86 0.86 0.93 0.82 0.94 0.87 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0 0.14 0.16 0.37 0.52 0.26 0.52 0.54 0.62 0.58 0 0 0 0 0 0 0.06 0 0.1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.08 0.06 0.07 0.08 0 0 0 0.17 0.2 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 5 0 3 5
Bud 0 1 1 2 5 3 26 16 18 22 17 25 0 0 0 0 0 1 0 0 1 0 8 13
Bud Neck 0 1 1 1 0 3 1 2 0 0 0 1 1 1 0 0 0 1 1 0 1 0 2 2
Bud Site 0 0 1 1 0 1 1 0 2 7 3 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 3 4 2 1 3 1 1 4 5 4 1 0 0 0 0 3 0 0 4 0 0 0
Cytoplasm 200 226 575 537 614 611 768 834 537 687 523 615 287 409 467 242 357 237 188 212 486 235 436 429
Endoplasmic Reticulum 1 0 2 0 0 1 2 1 1 1 3 1 0 1 0 5 28 10 0 0 2 1 2 1
Endosome 0 0 1 0 5 0 3 8 1 3 1 4 0 0 3 5 5 1 2 1 6 1 2 5
Golgi 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 2 1 0 0 0 5 0 2 4
Mitochondria 22 9 17 77 101 286 589 272 413 562 558 578 0 0 1 6 7 5 13 3 61 2 11 12
Nucleus 0 2 0 1 1 5 14 4 11 14 5 8 0 0 0 1 3 5 0 1 0 0 3 2
Nuclear Periphery 1 0 1 0 0 0 6 1 3 0 2 5 0 0 0 0 0 0 0 0 1 0 0 1
Nucleolus 0 0 0 0 2 0 1 4 2 1 0 5 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 3 0 2 1 1 11 1 2 3 2 0 1 1 0 0 0 2 0 4 0 6 12
Vac/Vac Membrane 1 2 3 6 16 32 43 34 64 63 60 79 1 3 7 48 79 41 2 2 3 2 9 7
Unique Cell Count 213 231 593 561 651 779 1123 1044 798 1048 903 993 289 412 474 284 404 274 220 229 595 251 499 507
Labelled Cell Count 225 241 610 629 748 945 1459 1188 1054 1366 1180 1330 291 415 479 309 481 304 220 229 595 251 499 507


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.0 3.8 3.5 4.0 3.3 3.3 3.7 3.3 3.2 3.0 3.0 5.3 5.4 5.6 6.0 6.1 5.3 5.4 5.6 5.7
Std Deviation (1e-4) 0.6 0.8 0.8 1.2 1.6 1.4 1.1 1.4 1.3 0.8 0.9 0.7 0.9 1.3 8.0 1.3 1.7 1.1 1.5 1.3 1.3
Intensity Change (Log2) – – – -0.11 0.05 -0.21 -0.2 -0.07 -0.21 -0.26 -0.37 -0.33 0.46 0.48 0.54 0.63 0.66 0.47 0.5 0.56 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 9.7 10.7 9.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6831 4.0247 3.9512 3.4698 3.7136 3.9429 -0.7607 -0.0194 -0.3701 -0.3777 -0.6409 -0.3444 -1.5279 -0.4159 -0.852 -0.5044 -1.0629 -1.102
Actin 0.0056 0.0007 0.0034 0.0007 0.0026 0.0016 0.0269 0.0002 0.0122 0.0001 0.0008 0.002 0.0029 0.0003 0.0002 0.0001 0.0006 0.0004
Bud 0.002 0.0009 0.0076 0.0031 0.0034 0.0043 0.0003 0.0037 0.0005 0 0.0004 0.0011 0 0.0002 0.0006 0.0001 0.0003 0.0002
Bud Neck 0.0003 0.0004 0.0023 0.0003 0.001 0.003 0.0035 0.0001 0.0022 0.0001 0.0202 0.0023 0.0001 0.0001 0.0017 0.0002 0.0001 0.0011
Bud Periphery 0.0035 0.001 0.0027 0.0064 0.0088 0.0059 0.0005 0.0008 0.0006 0 0.0004 0.0015 0 0.0001 0.0003 0 0.0005 0.0001
Bud Site 0.0022 0.0311 0.0097 0.0018 0.0007 0.0023 0.0021 0.0013 0.0022 0 0.0016 0.0003 0.0002 0.0026 0.009 0.0002 0.0001 0.0004
Cell Periphery 0.0004 0.0002 0.0002 0.0002 0.0004 0.0007 0.0001 0.0001 0.0001 0 0.0001 0.0001 0 0.0001 0.0001 0 0 0
Cytoplasm 0.065 0.0702 0.0774 0.054 0.1115 0.1143 0.2334 0.4212 0.3348 0.3261 0.2358 0.4152 0.225 0.3483 0.3404 0.462 0.4255 0.3876
Cytoplasmic Foci 0.0134 0.0152 0.008 0.0228 0.0137 0.0069 0.0184 0.0083 0.01 0.0065 0.0235 0.0161 0.0033 0.0032 0.0144 0.0084 0.01 0.012
Eisosomes 0.0003 0.0005 0.0002 0.0001 0.0003 0.0002 0.0002 0.0001 0.0002 0 0.0001 0 0.0001 0.0002 0.0001 0 0 0
Endoplasmic Reticulum 0.002 0.0007 0.002 0.0011 0.0017 0.0017 0.0031 0.0022 0.0017 0.0015 0.0014 0.0014 0.0015 0.0015 0.0017 0.0025 0.0037 0.0017
Endosome 0.0016 0.0003 0.0028 0.0007 0.001 0.0016 0.014 0.0015 0.0031 0.0028 0.0094 0.0049 0.0019 0.0008 0.0126 0.0084 0.0104 0.0032
Golgi 0.0004 0.0001 0.0007 0.0001 0.0002 0.0002 0.0044 0.0002 0.001 0.0001 0.0023 0.0004 0.0004 0.0001 0.0008 0.0001 0.0003 0.0002
Lipid Particles 0.0007 0.0003 0.0007 0.0011 0.0004 0.0005 0.0041 0.0004 0.0006 0.0001 0.0015 0.0003 0.0009 0.0001 0.0006 0.0001 0.0002 0.0003
Mitochondria 0.0008 0.0006 0.0011 0.001 0.0007 0.0007 0.0016 0.001 0.0068 0.0002 0.0006 0.0004 0.0003 0.0001 0.0004 0.0003 0.0003 0.0002
None 0.8732 0.8694 0.8579 0.8717 0.8368 0.8419 0.6591 0.5534 0.6143 0.6559 0.689 0.5383 0.7581 0.6386 0.6044 0.504 0.5383 0.5831
Nuclear Periphery 0.0041 0.0005 0.0047 0.0013 0.0009 0.002 0.0067 0.0006 0.001 0.0011 0.002 0.0016 0.0009 0.0006 0.0009 0.0025 0.0025 0.0008
Nucleolus 0.0012 0.0011 0.0018 0.0032 0.0008 0.0006 0.001 0.0003 0.0002 0.0001 0.0006 0.0003 0.0002 0.0001 0.0004 0.0002 0.0001 0.0004
Nucleus 0.0131 0.0018 0.0051 0.0046 0.0023 0.003 0.0031 0.002 0.0028 0.0026 0.0032 0.0072 0.0013 0.0019 0.0069 0.0062 0.0026 0.0032
Peroxisomes 0.0006 0.0002 0.0004 0.0004 0.0004 0.0002 0.0062 0.0003 0.0024 0.0001 0.0016 0.0006 0.0008 0.0001 0.0004 0.0001 0.0001 0.0002
Punctate Nuclear 0.0085 0.0046 0.0102 0.0238 0.011 0.0072 0.0099 0.0015 0.0026 0.0014 0.0032 0.0047 0.0018 0.0007 0.0009 0.0022 0.0011 0.0042
Vacuole 0.001 0.0003 0.0009 0.0012 0.001 0.0008 0.0009 0.0007 0.0007 0.0011 0.0019 0.0009 0.0002 0.0004 0.0031 0.002 0.0027 0.0005
Vacuole Periphery 0.0003 0 0.0003 0.0004 0.0002 0.0003 0.0006 0.0001 0.0002 0.0002 0.0003 0.0002 0.0001 0 0.0002 0.0004 0.0006 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1.2398 3.6943 3.8379 6.2375 6.4297 4.1303 7.4706 6.0506 5.5271 8.0853
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
108 370 1986 1289 1684 1165 350 1953 1792 1535 2336 3242

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 822.92 739.56 774.73 834.71 680.95 716.15 887.89 868.95 689.51 721.79 791.68 855.34
Standard Deviation 556.27 93.14 81.54 116.74 74.50 78.76 89.50 118.78 158.14 83.06 92.11 119.16
Intensity Change Log 2 — -0.154085 -0.087059 0.020523 — 0.072713 0.382832 0.351724 — -0.046957 0.144779 0.179958

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001176 0.000485 0.000098 0.000820 0.000063 0.000136 0.000249 0.001505 0.000130 0.000220 0.000120 0.001233
Bud Neck 0.010589 0.025198 0.003677 0.009733 0.008268 0.019474 0.001998 0.013985 0.008408 0.020854 0.003426 0.012294
Bud Site 0.009331 0.008495 0.007322 0.030142 0.003806 0.008749 0.005063 0.053212 0.004139 0.008688 0.006983 0.044040
Cell Periphery 0.000450 0.000166 0.000145 0.000210 0.000195 0.000189 0.000210 0.000272 0.000210 0.000183 0.000154 0.000247
Cytoplasm 0.201936* 0.342923* 0.376234* 0.384155* 0.506054* 0.435327* 0.198936* 0.472740* 0.487726* 0.413054* 0.349670* 0.437520*
Cytoplasmic Foci 0.063314 0.288914* 0.012768 0.017082 0.176534 0.203922* 0.026574 0.013134 0.169711 0.224409* 0.014836 0.014704
Eisosomes 0.000131 0.000170 0.000030 0.000052 0.000112 0.000146 0.000098 0.000045 0.000113 0.000152 0.000040 0.000048
Endoplasmic Reticulum 0.008547 0.001091 0.000716 0.004668 0.001061 0.000532 0.002492 0.002684 0.001512 0.000667 0.000982 0.003472
Endosome 0.008764 0.031589 0.001234 0.010481 0.005508 0.012479 0.003650 0.004851 0.005704 0.017086 0.001596 0.007090
Golgi 0.001314 0.004802 0.000103 0.004969 0.000810 0.002968 0.000124 0.002409 0.000840 0.003410 0.000106 0.003427
Lipid Particles 0.008846 0.012199 0.000819 0.002360 0.006253 0.008075 0.002977 0.001043 0.006410 0.009069 0.001142 0.001567
Mitochondria 0.003377 0.007360 0.000787 0.005442 0.003441 0.009963 0.000566 0.004918 0.003437 0.009336 0.000754 0.005126
Mitotic Spindle 0.021491 0.006314 0.002877 0.059660 0.002140 0.007713 0.001877 0.050332 0.003306 0.007376 0.002727 0.054041
None 0.015682 0.002144 0.005337 0.005769 0.004796 0.002049 0.007079 0.006193 0.005452 0.002072 0.005598 0.006024
Nuclear Periphery 0.002092 0.000684 0.001030 0.003772 0.000559 0.000537 0.001374 0.001631 0.000651 0.000573 0.001081 0.002482
Nuclear Periphery Foci 0.011125 0.001236 0.000188 0.003129 0.000322 0.000642 0.000737 0.000907 0.000973 0.000785 0.000270 0.001790
Nucleolus 0.038925 0.003950 0.000740 0.001042 0.001049 0.001690 0.000914 0.000750 0.003332 0.002234 0.000766 0.000866
Nucleus 0.548924* 0.158900* 0.568640* 0.325787* 0.251110* 0.219275* 0.669678* 0.281215* 0.269058* 0.204722* 0.583778* 0.298937*
Peroxisomes 0.003480 0.029077 0.000232 0.001518 0.002420 0.007113 0.001933 0.001442 0.002484 0.012407 0.000487 0.001472
Vacuole 0.037959 0.072308 0.016848 0.122176 0.024905 0.057570 0.073199 0.085372 0.025691 0.061122 0.025291 0.100005
Vacuole Periphery 0.002548 0.001995 0.000177 0.007030 0.000595 0.001450 0.000273 0.001360 0.000713 0.001581 0.000191 0.003614

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.80 4.51 1.92 -1.43 -4.91 -3.26 -3.07 -4.60 -4.13 -2.56 -2.57 -0.48 -5.74 -4.87 -5.58
Bud Neck -3.04 2.52 0.98 4.38 -4.33 -5.52 7.29 -3.24 3.78 -11.55 -6.70 5.59 -2.01 5.88 -9.42
Bud Site 0.52 0.89 -2.23 -3.89 -4.50 -2.89 -0.54 -9.75 -6.89 -7.72 -3.11 -1.86 -10.93 -7.60 -8.62
Cell Periphery 4.01 4.37 4.26 0.88 -0.78 0.12 -0.32 0.69 1.19 1.35 0.58 1.20 1.14 1.59 -0.08
Cytoplasm -5.30 -6.99 -4.63 1.99 5.84 7.42 26.76 16.69 7.48 -14.65 8.48 18.27 19.28 8.76 -0.68
Cytoplasmic Foci -14.81 5.30 5.13 23.91 -1.22 -3.81 29.41 35.56 34.17 4.68 -8.03 34.82 35.55 41.01 1.21
Eisosomes -1.61 7.39 4.41 7.30 -14.48 -4.32 -0.19 9.52 14.84 5.86 -5.17 12.54 9.16 15.86 -7.74
Endoplasmic Reticulum 3.28 3.36 1.27 -13.11 -15.54 2.54 -6.88 -6.72 -9.96 1.03 3.48 0.69 -8.89 -16.80 -13.63
Endosome -4.74 3.76 1.55 6.12 -6.55 -6.84 1.49 3.76 10.33 1.23 -8.58 6.96 2.46 10.35 -6.38
Golgi -3.61 3.71 -0.28 3.07 -2.75 -2.86 8.46 -0.06 2.70 -2.79 -4.16 8.80 -1.05 3.38 -3.86
Lipid Particles -1.14 4.04 3.26 5.98 -9.45 -2.44 5.79 11.75 10.11 6.53 -3.55 12.65 11.29 11.37 -5.20
Mitochondria -2.60 2.54 0.74 3.90 -4.32 -3.33 3.28 1.08 4.21 -4.59 -3.68 3.27 1.15 4.93 -5.62
Mitotic Spindle 1.79 2.08 -0.86 -7.08 -8.78 -2.86 0.04 -11.21 -7.94 -11.09 -2.13 0.55 -12.97 -10.33 -13.85
None 2.38 1.84 1.95 -3.95 0.95 4.15 -0.41 -2.90 -11.37 -0.75 4.75 0.31 -0.78 -11.60 -1.51
Nuclear Periphery 1.54 1.00 -3.42 -13.51 -12.84 0.30 -10.45 -22.61 -23.75 -12.03 0.70 -7.82 -22.43 -24.61 -19.88
Nuclear Periphery Foci 1.87 2.06 1.43 -4.37 -10.24 -0.95 -5.84 -7.23 -2.94 -2.87 0.44 1.86 -4.08 -5.26 -11.85
Nucleolus 4.01 4.42 4.33 3.03 -2.49 -2.02 1.05 -0.96 0.81 -2.16 1.74 4.27 3.10 2.71 -2.94
Nucleus 11.61 -1.28 6.19 -14.42 23.43 4.02 -25.05 -13.09 -15.95 17.77 8.45 -39.21 -11.37 -20.60 30.27
Peroxisomes -5.59 4.10 3.38 6.38 -1.98 -6.04 0.77 6.39 8.19 1.04 -8.00 8.19 7.08 9.47 -1.72
Vacuole -3.12 2.38 -16.07 -15.92 -29.42 -11.38 -9.28 -27.51 -19.15 -5.14 -13.07 -4.15 -38.50 -26.58 -34.51
Vacuole Periphery 0.51 2.34 0.10 -0.53 -3.04 -3.41 3.25 0.36 3.19 -1.46 -3.93 5.22 -1.72 0.88 -3.52
Standard name
Human Ortholog
Description Possible shared subunit of Cop9 signalosome (CSN) and eIF3; binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain
Localization
Cell Percentages cytoplasm (30%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pci8

Pci8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pci8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available