| Standard name | |
|---|---|
| Description | Possible shared subunit of Cop9 signalosome (CSN) and eIF3; binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 0 | 3 | 5 |
| Bud | 0 | 1 | 1 | 2 | 5 | 3 | 26 | 16 | 18 | 22 | 17 | 25 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 8 | 13 |
| Bud Neck | 0 | 1 | 1 | 1 | 0 | 3 | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 2 |
| Bud Site | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 2 | 7 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 0 | 0 | 3 | 4 | 2 | 1 | 3 | 1 | 1 | 4 | 5 | 4 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 4 | 0 | 0 | 0 |
| Cytoplasm | 200 | 226 | 575 | 537 | 614 | 611 | 768 | 834 | 537 | 687 | 523 | 615 | 287 | 409 | 467 | 242 | 357 | 237 | 188 | 212 | 486 | 235 | 436 | 429 |
| Endoplasmic Reticulum | 1 | 0 | 2 | 0 | 0 | 1 | 2 | 1 | 1 | 1 | 3 | 1 | 0 | 1 | 0 | 5 | 28 | 10 | 0 | 0 | 2 | 1 | 2 | 1 |
| Endosome | 0 | 0 | 1 | 0 | 5 | 0 | 3 | 8 | 1 | 3 | 1 | 4 | 0 | 0 | 3 | 5 | 5 | 1 | 2 | 1 | 6 | 1 | 2 | 5 |
| Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 5 | 0 | 2 | 4 |
| Mitochondria | 22 | 9 | 17 | 77 | 101 | 286 | 589 | 272 | 413 | 562 | 558 | 578 | 0 | 0 | 1 | 6 | 7 | 5 | 13 | 3 | 61 | 2 | 11 | 12 |
| Nucleus | 0 | 2 | 0 | 1 | 1 | 5 | 14 | 4 | 11 | 14 | 5 | 8 | 0 | 0 | 0 | 1 | 3 | 5 | 0 | 1 | 0 | 0 | 3 | 2 |
| Nuclear Periphery | 1 | 0 | 1 | 0 | 0 | 0 | 6 | 1 | 3 | 0 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
| Nucleolus | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 4 | 2 | 1 | 0 | 5 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
| SpindlePole | 0 | 0 | 3 | 0 | 2 | 1 | 1 | 11 | 1 | 2 | 3 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 4 | 0 | 6 | 12 |
| Vac/Vac Membrane | 1 | 2 | 3 | 6 | 16 | 32 | 43 | 34 | 64 | 63 | 60 | 79 | 1 | 3 | 7 | 48 | 79 | 41 | 2 | 2 | 3 | 2 | 9 | 7 |
| Unique Cell Count | 213 | 231 | 593 | 561 | 651 | 779 | 1123 | 1044 | 798 | 1048 | 903 | 993 | 289 | 412 | 474 | 284 | 404 | 274 | 220 | 229 | 595 | 251 | 499 | 507 |
| Labelled Cell Count | 225 | 241 | 610 | 629 | 748 | 945 | 1459 | 1188 | 1054 | 1366 | 1180 | 1330 | 291 | 415 | 479 | 309 | 481 | 304 | 220 | 229 | 595 | 251 | 499 | 507 |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 3.3 | 4.0 | 3.8 | 3.5 | 4.0 | 3.3 | 3.3 | 3.7 | 3.3 | 3.2 | 3.0 | 3.0 | 5.3 | 5.4 | 5.6 | 6.0 | 6.1 | 5.3 | 5.4 | 5.6 | 5.7 |
| Std Deviation (1e-4) | 0.6 | 0.8 | 0.8 | 1.2 | 1.6 | 1.4 | 1.1 | 1.4 | 1.3 | 0.8 | 0.9 | 0.7 | 0.9 | 1.3 | 8.0 | 1.3 | 1.7 | 1.1 | 1.5 | 1.3 | 1.3 |
| Intensity Change (Log2) | – | – | – | -0.11 | 0.05 | -0.21 | -0.2 | -0.07 | -0.21 | -0.26 | -0.37 | -0.33 | 0.46 | 0.48 | 0.54 | 0.63 | 0.66 | 0.47 | 0.5 | 0.56 | 0.57 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 9.7 | 10.7 | 9.0 |
External localization resources
