Standard name
Description Possible shared subunit of Cop9 signalosome (CSN) and eIF3; binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.98 0.97 0.96 0.94 0.78 0.68 0.8 0.67 0.66 0.58 0.62 0.99 0.99 0.99 0.85 0.88 0.86 0.86 0.93 0.82 0.94 0.87 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0 0.14 0.16 0.37 0.52 0.26 0.52 0.54 0.62 0.58 0 0 0 0 0 0 0.06 0 0.1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.08 0.06 0.07 0.08 0 0 0 0.17 0.2 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 5 0 3 5
Bud 0 1 1 2 5 3 26 16 18 22 17 25 0 0 0 0 0 1 0 0 1 0 8 13
Bud Neck 0 1 1 1 0 3 1 2 0 0 0 1 1 1 0 0 0 1 1 0 1 0 2 2
Bud Site 0 0 1 1 0 1 1 0 2 7 3 3 0 0 0 0 0 0
Cell Periphery 0 0 3 4 2 1 3 1 1 4 5 4 1 0 0 0 0 3 0 0 4 0 0 0
Cytoplasm 200 226 575 537 614 611 768 834 537 687 523 615 287 409 467 242 357 237 188 212 486 235 436 429
Endoplasmic Reticulum 1 0 2 0 0 1 2 1 1 1 3 1 0 1 0 5 28 10 0 0 2 1 2 1
Endosome 0 0 1 0 5 0 3 8 1 3 1 4 0 0 3 5 5 1 2 1 6 1 2 5
Golgi 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 2 1 0 0 0 5 0 2 4
Mitochondria 22 9 17 77 101 286 589 272 413 562 558 578 0 0 1 6 7 5 13 3 61 2 11 12
Nucleus 0 2 0 1 1 5 14 4 11 14 5 8 0 0 0 1 3 5 0 1 0 0 3 2
Nuclear Periphery 1 0 1 0 0 0 6 1 3 0 2 5 0 0 0 0 0 0 0 0 1 0 0 1
Nucleolus 0 0 0 0 2 0 1 4 2 1 0 5 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 3 0 2 1 1 11 1 2 3 2 0 1 1 0 0 0 2 0 4 0 6 12
Vac/Vac Membrane 1 2 3 6 16 32 43 34 64 63 60 79 1 3 7 48 79 41 2 2 3 2 9 7
Unique Cell Count 213 231 593 561 651 779 1123 1044 798 1048 903 993 289 412 474 284 404 274 220 229 595 251 499 507
Labelled Cell Count 225 241 610 629 748 945 1459 1188 1054 1366 1180 1330 291 415 479 309 481 304 220 229 595 251 499 507


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.0 3.8 3.5 4.0 3.3 3.3 3.7 3.3 3.2 3.0 3.0 5.3 5.4 5.6 6.0 6.1 5.3 5.4 5.6 5.7
Std Deviation (1e-4) 0.6 0.8 0.8 1.2 1.6 1.4 1.1 1.4 1.3 0.8 0.9 0.7 0.9 1.3 8.0 1.3 1.7 1.1 1.5 1.3 1.3
Intensity Change (Log2) -0.11 0.05 -0.21 -0.2 -0.07 -0.21 -0.26 -0.37 -0.33 0.46 0.48 0.54 0.63 0.66 0.47 0.5 0.56 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 9.7 10.7 9.0