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Description | Lysine methyltransferase; involved in the dimethylation of eEF1A (Tef1p/Tef2p) at lysine 316; sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 |
Bud | 1 | 0 | 0 | 2 | 1 | 3 | 4 | 0 | 14 | 4 | 11 | 7 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 8 | 11 | 6 | 6 | 17 | 17 | 5 | 11 | 6 | 11 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 4 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 2 | 4 | 4 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 5 | 2 | 3 | 1 | 2 | 3 | 0 | 2 | 0 | 2 | – | 0 | 0 | 8 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 48 | 90 | 298 | 342 | 465 | 401 | 497 | 441 | 329 | 286 | 177 | 317 | – | 17 | 86 | 86 | 164 | 129 | 60 | 88 | 276 | 18 | 39 | 55 |
Endoplasmic Reticulum | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | 1 | 0 | 5 | 5 | 5 | 0 | 0 | 1 | 0 | 0 | 0 |
Endosome | 0 | 2 | 1 | 3 | 1 | 2 | 2 | 3 | 3 | 0 | 2 | 4 | – | 0 | 2 | 0 | 0 | 1 | 1 | 2 | 2 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 0 |
Mitochondria | 6 | 13 | 1 | 7 | 6 | 42 | 63 | 40 | 146 | 118 | 139 | 172 | – | 0 | 1 | 2 | 3 | 0 | 3 | 7 | 15 | 0 | 0 | 2 |
Nucleus | 0 | 1 | 1 | 2 | 1 | 1 | 4 | 3 | 9 | 6 | 10 | 11 | – | 0 | 1 | 1 | 0 | 3 | 0 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 0 | 2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 3 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 2 | 1 | 8 | 8 | 16 | 14 | 14 | 17 | 14 | 20 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 1 |
Vac/Vac Membrane | 1 | 1 | 5 | 6 | 9 | 4 | 6 | 5 | 8 | 4 | 1 | 4 | – | 0 | 2 | 0 | 0 | 1 | 2 | 9 | 3 | 0 | 1 | 2 |
Unique Cell Count | 55 | 101 | 311 | 358 | 481 | 445 | 566 | 499 | 459 | 388 | 302 | 459 | 18 | 91 | 88 | 164 | 133 | 75 | 122 | 321 | 22 | 45 | 67 | |
Labelled Cell Count | 57 | 111 | 323 | 376 | 503 | 468 | 613 | 530 | 536 | 454 | 365 | 558 | 18 | 93 | 102 | 176 | 144 | 75 | 122 | 321 | 22 | 45 | 67 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.8 | 4.1 | 5.4 | 5.3 | 5.4 | 4.2 | 4.1 | 4.5 | 3.5 | 3.6 | 3.3 | 3.5 | – | 4.8 | 5.3 | 7.3 | 8.5 | 9.4 | – | – | 6.9 |
Std Deviation (1e-4) | 0.4 | 0.9 | 1.3 | 1.9 | 1.3 | 1.3 | 1.1 | 1.7 | 1.2 | 1.0 | 0.8 | 0.8 | – | 1.2 | 1.8 | 1.0 | 1.3 | 1.7 | – | – | 3.4 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.01 | -0.37 | -0.39 | -0.25 | -0.64 | -0.6 | -0.7 | -0.6 | – | -0.18 | -0.04 | 0.44 | 0.65 | 0.79 | – | – | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |