Standard name
Description Lysine methyltransferase; involved in the dimethylation of eEF1A (Tef1p/Tef2p) at lysine 316; sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.09 0 0 0 0 0 0 0 0
Cytoplasm 0.87 0.89 0.96 0.96 0.97 0.9 0.88 0.88 0.72 0.74 0.59 0.69 – 0.94 0.95 0.98 1.0 0.97 0.81 0.73 0.86 0.85 0.87 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.13 0 0 0 0.09 0.11 0.08 0.32 0.3 0.46 0.37 – 0 0 0 0 0 0.05 0.06 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.08 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 1 0 0 – 0 0 0 0 0 0 1 3 0 0 0
Bud 1 0 0 2 1 3 4 0 14 4 11 7 – 0 0 0 0 0 0 0 1 0 0 1
Bud Neck 0 0 8 11 6 6 17 17 5 11 6 11 – 0 0 0 0 0 2 0 4 0 0 0
Bud Site 0 0 0 0 0 0 0 0 6 2 4 4 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 5 2 3 1 2 3 0 2 0 2 – 0 0 8 4 4 0 0 0 0 0 0
Cytoplasm 48 90 298 342 465 401 497 441 329 286 177 317 – 17 86 86 164 129 60 88 276 18 39 55
Endoplasmic Reticulum 0 0 2 0 0 0 0 0 0 1 1 0 – 1 0 5 5 5 0 0 1 0 0 0
Endosome 0 2 1 3 1 2 2 3 3 0 2 4 – 0 2 0 0 1 1 2 2 0 0 0
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 – 0 0 0 0 1 0 1 2 0 0 0
Mitochondria 6 13 1 7 6 42 63 40 146 118 139 172 – 0 1 2 3 0 3 7 15 0 0 2
Nucleus 0 1 1 2 1 1 4 3 9 6 10 11 – 0 1 1 0 3 0 2 0 0 0 0
Nuclear Periphery 0 1 0 0 2 0 0 1 0 0 0 1 – 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 1 2 2 1 0 2 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 0 0 0 0 1 0 0 1 0 3 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 2 1 8 8 16 14 14 17 14 20 – 0 0 0 0 0 1 1 2 0 0 1
Vac/Vac Membrane 1 1 5 6 9 4 6 5 8 4 1 4 – 0 2 0 0 1 2 9 3 0 1 2
Unique Cell Count 55 101 311 358 481 445 566 499 459 388 302 459 18 91 88 164 133 75 122 321 22 45 67
Labelled Cell Count 57 111 323 376 503 468 613 530 536 454 365 558 18 93 102 176 144 75 122 321 22 45 67


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.1 5.4 5.3 5.4 4.2 4.1 4.5 3.5 3.6 3.3 3.5 – 4.8 5.3 7.3 8.5 9.4 – – 6.9
Std Deviation (1e-4) 0.4 0.9 1.3 1.9 1.3 1.3 1.1 1.7 1.2 1.0 0.8 0.8 – 1.2 1.8 1.0 1.3 1.7 – – 3.4
Intensity Change (Log2) – – – -0.03 0.01 -0.37 -0.39 -0.25 -0.64 -0.6 -0.7 -0.6 – -0.18 -0.04 0.44 0.65 0.79 – – 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –