Standard name
Description Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0 0.08 0.06 0.08 0.05 0.07 0 0 0.12 0.21 0.22 0.14 0.16 0.13 0 0 0 0 0.06 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.14 0.08 0 0 0 0 0 0
Mitochondria 0.1 0 0 0.05 0.06 0.22 0.21 0.42 0.29 0.58 0.44 0 0 0 0 0 0 0 0 0 0.05 0 0
Nucleus 0.75 0.6 0.78 0.88 0.69 0.66 0.69 0.67 0.76 0.67 0.72 0.69 0.6 0.63 0.51 0.43 0.46 0.65 0.62 0.59 0.58 0.5 0.42
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0.24 0.4 0.19 0.07 0.17 0.15 0.13 0.07 0.05 0 0 0.22 0.21 0.14 0.35 0.32 0.42 0.21 0.21 0.25 0.23 0.24 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1 0 0 0 0 0 0 0
Bud 0 0 0 1 0 0 1 2 0 1 1 0 0 0 1 1 0 3 6 4 3 5 11
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 4
Bud Site 0 0 0 0 0 0 1 6 2 5 5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0
Cytoplasm 1 8 7 3 10 9 18 12 17 7 5 20 47 45 51 46 33 1 4 2 5 16 15
Endoplasmic Reticulum 0 0 0 0 0 1 0 0 1 0 0 0 0 1 18 9 15 0 1 0 1 0 4
Endosome 3 2 3 0 3 3 9 0 0 0 2 3 9 9 6 2 1 8 5 4 3 8 5
Golgi 1 1 1 0 0 0 0 0 0 0 0 2 0 0 21 39 22 0 1 1 0 0 1
Mitochondria 17 4 0 6 7 33 45 95 67 93 91 1 0 0 2 6 4 3 3 2 9 9 10
Nucleus 133 106 91 113 84 101 147 151 177 106 149 120 133 130 179 124 120 149 135 82 120 126 121
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0 0 1 0 0 0 1 1
Nucleolus 2 0 0 4 2 5 1 2 6 6 7 0 0 1 0 2 1 9 6 1 5 6 8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 1 0 0 1 2 5 2 5 0 6 0 3 1 0 0 0 3 3 1 7 9 14
Vac/Vac Membrane 42 71 22 9 21 23 27 15 11 5 9 38 46 28 124 91 109 47 46 35 47 61 76
Unique Cell Count 177 178 117 129 122 152 213 224 233 159 208 173 221 206 354 286 261 230 220 141 209 253 287
Labelled Cell Count 201 193 124 136 128 177 254 286 287 223 277 185 239 217 404 321 305 230 220 141 209 253 287


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 7.7 8.3 7.2 6.8 5.6 5.4 4.6 4.8 4.4 4.5 8.0 7.7 11.1 16.3 16.0 15.9 6.9 7.2 7.6
Std Deviation (1e-4) 2.0 2.1 2.3 1.8 1.9 1.4 1.3 0.9 0.9 1.6 1.0 1.5 1.6 30.1 4.1 3.8 3.7 1.5 1.6 1.7
Intensity Change (Log2) -0.22 -0.3 -0.58 -0.62 -0.87 -0.8 -0.92 -0.9 -0.06 -0.12 0.41 0.97 0.94 0.93 -0.27 -0.22 -0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.7 0 0.8 -0.2 0.5 -0.6 0 1.6 3.6 3.7 2.4 2.7 1.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 2.3 3.9 2.8
Mitochondria 0 0 5.4 5.3 0 0 0 0 0 0 0 0 0 0
Nucleus 2.0 -1.6 -2.0 -1.7 -2.0 -0.4 -2.0 -1.2 -1.6 -3.3 -2.7 -5.2 -6.3 -5.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.8 -0.3 -0.8 -1.5 -3.4 -4.3 -4.3 -4.3 0.7 0.4 -1.2 3.3 2.6 4.3


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vhr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available