Standard name
Human Ortholog
Description Mitochondrial protein kinase; involved in negative regulation of pyruvate dehydrogenase complex activity by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp2p and phosphatases Ptc5p and Ptc6p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.06 0 0.07 0.06 0.06 0 0.05 0 0.06 0 0 0 0 0 0 0.06 0.07 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Cytoplasm 0.1 0.06 0 0.07 0 0 0 0 0 0 0 0 0.17 0.17 0.2 0.12 0.1 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0.08 0.05 0.12 0 0 0 0 0 0 0 0.24 0.29 0.28 0.2 0.23 0.18 0.13 0.19 0.1 0.11 0.12 0.11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05
Mitochondria 0.85 0.94 0.65 0.79 0.83 0.94 0.92 0.89 0.94 0.96 0.96 0.95 0.29 0.11 0.19 0.2 0.11 0.13 0.67 0.56 0.63 0.6 0.63 0.56
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.07 0.05 0.05 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.05 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.07 0 0.21 0.07 0.16 0 0 0 0 0 0 0 0.23 0.24 0.41 0.19 0.21 0.29 0.06 0.07 0.12 0.07 0.05 0.09
SpindlePole 0.1 0.05 0.18 0.1 0.09 0 0 0 0.05 0 0 0 0.15 0.24 0.21 0.36 0.31 0.34 0 0 0 0 0 0.05
Vac/Vac Membrane 0.06 0.07 0 0 0.07 0 0 0 0.05 0 0 0 0.13 0.15 0.08 0.13 0.13 0.11 0 0 0 0.05 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 2 0 0 0 0 1 0 0 0 1 2 0 0 2 1 2 8 1 8 4 10 13
Bud 26 3 20 11 20 11 28 21 27 22 14 14 5 13 11 19 16 9 1 0 0 3 3 3
Bud Neck 1 0 2 0 1 0 1 0 0 0 0 0 1 1 0 0 13 2 2 0 0 2 2 5
Bud Site 8 1 3 2 2 8 12 6 10 3 6 4 0 2 0 9 2 8
Cell Periphery 4 0 10 1 1 2 4 0 5 2 2 1 5 7 10 21 12 1 0 0 0 0 0 0
Cytoplasm 42 6 12 13 13 11 10 7 7 4 5 4 40 56 86 39 23 22 1 0 1 4 3 1
Endoplasmic Reticulum 0 3 5 2 0 2 2 1 1 2 0 4 6 0 1 11 15 5 0 0 0 1 1 5
Endosome 9 0 25 9 42 1 3 15 3 0 2 2 58 94 121 67 54 32 63 21 33 34 52 52
Golgi 0 0 0 2 0 0 0 0 0 0 1 0 3 4 2 7 8 7 11 3 13 13 17 24
Mitochondria 373 96 193 138 289 322 527 530 398 501 432 399 70 37 82 64 25 24 314 61 203 180 279 262
Nucleus 6 0 0 0 1 2 4 13 3 5 1 2 2 1 0 3 4 3 0 1 0 2 3 2
Nuclear Periphery 0 0 3 0 4 14 39 27 21 19 19 27 0 0 0 0 0 2 5 0 1 1 3 4
Nucleolus 1 2 0 0 7 11 31 29 17 29 15 17 0 1 1 4 1 2 1 1 0 1 4 5
Peroxisomes 31 0 62 12 54 9 17 16 8 8 5 3 54 76 178 62 49 52 29 7 40 19 20 40
SpindlePole 44 5 54 17 30 12 25 25 21 14 13 8 36 79 91 117 74 60 14 4 10 11 16 22
Vac/Vac Membrane 25 7 13 7 23 10 8 18 21 14 19 12 32 50 34 42 30 19 11 2 1 14 22 17
Unique Cell Count 440 102 296 174 347 344 570 595 422 523 452 419 240 323 429 328 236 179 473 109 322 302 444 470
Labelled Cell Count 570 125 404 214 487 415 711 709 542 623 534 498 314 421 617 467 327 250 473 109 322 302 444 470


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.4 4.7 4.9 5.1 4.4 4.4 4.8 4.8 4.5 4.4 4.7 6.2 5.9 6.0 6.5 6.2 6.5 6.0 6.4 6.9
Std Deviation (1e-4) 0.6 0.8 1.5 1.8 1.9 1.3 1.1 1.2 1.2 1.1 1.2 1.2 1.2 1.2 1.3 1.5 1.6 1.9 1.3 1.6 1.5
Intensity Change (Log2) 0.05 0.12 -0.08 -0.08 0.03 0.03 -0.04 -0.09 0.01 0.4 0.34 0.35 0.46 0.4 0.47 0.36 0.45 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 3.7 3.5 2.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3091 2.3018 2.3778 2.0997 2.1982 2.4318 3.7936 3.6269 3.7766 3.3707 3.8477 3.6251 3.6591 3.2498 2.9638 3.2978 2.8704 3.18
Actin 0.0033 0.0028 0.0071 0.004 0.0005 0.0028 0.0092 0.0014 0.0059 0.0016 0.0082 0.0032 0.0146 0.0087 0.0162 0.0023 0.0025 0.0026
Bud 0.0001 0.0054 0.0007 0.002 0.0001 0.0001 0.0003 0.0003 0.0004 0.0001 0.0026 0.0001 0.0002 0.0011 0.0003 0.0001 0.0003 0.0001
Bud Neck 0.0002 0.0001 0.0005 0 0.0001 0.0001 0.0004 0.0001 0.0003 0.0001 0.0007 0.0001 0.0002 0.0002 0.0004 0.0001 0.0001 0.0001
Bud Periphery 0.0003 0.0012 0.0008 0.0112 0.0003 0.0002 0.0005 0.0005 0.0007 0.0005 0.0051 0.0003 0.0004 0.0017 0.0004 0.0001 0.0008 0.0002
Bud Site 0.0004 0.0015 0.0033 0.0002 0.0001 0.0001 0.0007 0.0006 0.0032 0.0002 0.0063 0.0001 0.0003 0.0039 0.0012 0.0001 0.0002 0.0001
Cell Periphery 0.0001 0.0001 0.0004 0.0002 0.0001 0.0001 0.0002 0.0001 0.0003 0.0001 0.0003 0.0001 0.0001 0.0015 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.0004 0.0005 0.0007 0.0001 0.0001 0.0001 0.0027 0.0004 0.001 0.0001 0.0031 0.0005 0.0002 0.0036 0.0031 0.0001 0.0001 0.0001
Cytoplasmic Foci 0.0084 0.0065 0.0123 0.0084 0.0033 0.0072 0.0123 0.0134 0.0059 0.0014 0.0134 0.0055 0.0068 0.0151 0.0101 0.0017 0.0046 0.0047
Eisosomes 0.0002 0.0001 0.0005 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0002 0.0001 0.0002 0.0002 0.0002 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0015 0.0002 0.0007 0.0004 0.0013 0.0005 0.0003 0.0004 0.0005 0.0001 0.0001 0.0004
Endosome 0.0186 0.0141 0.0084 0.0027 0.0068 0.0137 0.0423 0.0443 0.0184 0.0135 0.0365 0.0252 0.0203 0.0336 0.0161 0.0313 0.0167 0.0144
Golgi 0.0238 0.0235 0.0115 0.0042 0.0096 0.023 0.0425 0.0487 0.0197 0.0108 0.0542 0.0492 0.0482 0.0597 0.0273 0.0292 0.045 0.0396
Lipid Particles 0.0289 0.007 0.0053 0.0099 0.0028 0.0109 0.0145 0.0066 0.0035 0.0048 0.0032 0.0095 0.0319 0.0187 0.0064 0.0014 0.012 0.0046
Mitochondria 0.8602 0.8977 0.9157 0.9408 0.94 0.9079 0.8426 0.8509 0.915 0.9569 0.838 0.8898 0.8551 0.8162 0.8887 0.8904 0.9097 0.9088
None 0.0034 0.0001 0.0018 0.0001 0.0001 0.0002 0.0008 0.0001 0.0003 0.0001 0.005 0.0001 0.0003 0.0026 0.0007 0 0.0001 0.0001
Nuclear Periphery 0.0003 0.0001 0.0009 0.0001 0 0.0001 0.0037 0.0001 0.0036 0.0006 0.0025 0.0007 0.0015 0.0001 0.0004 0.0001 0.0001 0.002
Nucleolus 0.0002 0.0001 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0005 0.0001 0.0001 0.0002 0.0007 0.0001 0.0001 0.0001
Nucleus 0.0001 0.0009 0.0003 0.0001 0 0 0.0013 0.0001 0.0008 0.0002 0.0015 0.0001 0.0001 0.0002 0.0004 0.0001 0.0001 0.0002
Peroxisomes 0.0438 0.0365 0.0272 0.0143 0.0349 0.0315 0.0159 0.0263 0.0139 0.0028 0.0078 0.0121 0.0152 0.0277 0.0241 0.0082 0.0043 0.0189
Punctate Nuclear 0.0003 0.0001 0.0003 0 0 0.0001 0.0028 0 0.0005 0 0.0005 0.0001 0.0003 0.0001 0.0006 0 0 0.0001
Vacuole 0.0038 0.0004 0.001 0.0006 0.0004 0.0007 0.0021 0.0029 0.0024 0.0021 0.0039 0.0009 0.0012 0.0025 0.001 0.0247 0.001 0.0007
Vacuole Periphery 0.003 0.0012 0.0008 0.0008 0.0005 0.0012 0.0033 0.0027 0.0032 0.0035 0.0053 0.0016 0.0024 0.002 0.0012 0.0098 0.0021 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.1901 4.1619 3.706 7.0688 9.6581 7.445 9.2579 11.6448 11.2674 10.0989
Translational Efficiency 0.9749 0.9855 0.9113 0.4997 0.4885 0.711 0.3935 0.5534 0.6067 0.5327

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1371 293 423 978 1668 1267 84 1007 3039 1560 507 1985

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 688.91 845.21 893.13 805.94 691.72 756.61 1031.74 899.92 690.45 773.25 916.09 853.62
Standard Deviation 80.07 101.70 113.58 124.13 84.87 100.84 118.05 129.11 82.75 106.77 125.41 135.11
Intensity Change Log 2 0.294994 0.374555 0.226357 0.129362 0.576819 0.379609 0.214385 0.479434 0.305173

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001223 0.001387 0.005110 0.030843 0.002092 0.001984 0.007739 0.032223 0.001700 0.001872 0.005546 0.031543
Bud Neck 0.009714 0.008819 0.033100 0.009201 0.003657 0.003818 0.015966 0.007787 0.006389 0.004758 0.030261 0.008483
Bud Site 0.010945 0.021626 0.030041 0.099459 0.006918 0.007190 0.037176 0.108290* 0.008735 0.009901 0.031223 0.103939*
Cell Periphery 0.000449 0.000237 0.000290 0.001026 0.000717 0.000268 0.000317 0.001093 0.000596 0.000262 0.000295 0.001060
Cytoplasm 0.008114 0.006517 0.017834 0.020322 0.005253 0.004621 0.006144 0.024145 0.006544 0.004977 0.015897 0.022261
Cytoplasmic Foci 0.074406 0.089616 0.112817 0.072388 0.074860 0.074393 0.124383 0.103690 0.074655 0.077253 0.114733 0.088268
Eisosomes 0.000124 0.000091 0.000209 0.000246 0.000160 0.000103 0.000169 0.000244 0.000144 0.000101 0.000202 0.000245
Endoplasmic Reticulum 0.000549 0.000736 0.003991 0.002624 0.000649 0.000252 0.005542 0.001570 0.000604 0.000343 0.004248 0.002090
Endosome 0.091753 0.161741 0.123936 0.075307 0.085218 0.118667 0.125217 0.082389 0.088166 0.126757 0.124148 0.078900
Golgi 0.151381* 0.129567* 0.061632 0.108216 0.169446* 0.170081* 0.070867 0.158249* 0.161296* 0.162472* 0.063162 0.133598*
Lipid Particles 0.007543 0.005004 0.041190 0.001925 0.007792 0.004391 0.008049 0.000786 0.007680 0.004506 0.035700 0.001347
Mitochondria 0.545284* 0.379083* 0.197103* 0.349621* 0.546541* 0.504333* 0.164239* 0.244231* 0.545974* 0.480809* 0.191658* 0.296156*
Mitotic Spindle 0.008392 0.047238 0.134041* 0.041543 0.008405 0.015471 0.092350 0.052386 0.008399 0.021437 0.127133* 0.047044
None 0.000280 0.000395 0.001217 0.000259 0.000283 0.000382 0.000209 0.000198 0.000282 0.000385 0.001050 0.000228
Nuclear Periphery 0.000168 0.000168 0.002269 0.000115 0.000086 0.000073 0.002073 0.000288 0.000123 0.000091 0.002237 0.000203
Nuclear Periphery Foci 0.000251 0.000391 0.002148 0.001181 0.000395 0.000222 0.002456 0.000484 0.000330 0.000254 0.002199 0.000827
Nucleolus 0.000552 0.002157 0.011943 0.000378 0.000656 0.000377 0.007226 0.000407 0.000609 0.000711 0.011161 0.000392
Nucleus 0.001854 0.001876 0.034409 0.001550 0.000961 0.000622 0.017033 0.001319 0.001364 0.000857 0.031530 0.001433
Peroxisomes 0.068310 0.108414* 0.086520 0.143962* 0.072221 0.073754 0.218499* 0.141234* 0.070456 0.080264 0.108387* 0.142578*
Vacuole 0.007283 0.022611 0.077905 0.028757 0.005353 0.009841 0.072186 0.028921 0.006224 0.012239 0.076958 0.028840
Vacuole Periphery 0.011426 0.012328 0.022294 0.011079 0.008335 0.009156 0.022160 0.010066 0.009729 0.009752 0.022272 0.010565

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.45 -3.47 -10.80 -10.70 -8.75 0.26 -2.93 -10.67 -10.68 -7.12 -0.55 -3.92 -15.15 -14.99 -11.89
Bud Neck 0.37 -5.35 0.26 -0.19 5.56 -0.20 -3.32 -4.35 -3.88 2.13 1.86 -6.63 -2.32 -4.27 6.02
Bud Site -2.31 -5.72 -15.77 -10.93 -10.82 -0.25 -5.29 -17.83 -17.79 -8.57 -0.96 -7.88 -23.85 -23.10 -14.82
Cell Periphery 2.95 2.44 -2.87 -4.06 -3.84 1.92 1.65 -1.20 -4.05 -3.66 2.52 2.29 -2.42 -5.66 -5.45
Cytoplasm 0.75 -2.99 -6.44 -6.01 -0.60 0.53 -1.59 -9.55 -9.30 -6.67 1.48 -3.61 -11.55 -11.73 -1.96
Cytoplasmic Foci -2.23 -5.36 0.59 2.54 5.59 0.12 -3.24 -6.28 -6.16 1.30 -0.77 -6.30 -4.29 -3.05 4.18
Eisosomes 3.73 -4.60 -9.20 -11.82 -1.72 4.09 -1.05 -4.47 -9.49 -2.46 4.78 -3.69 -8.63 -14.10 -2.22
Endoplasmic Reticulum -0.74 -5.30 -4.16 -3.42 1.81 2.87 -3.38 -3.04 -4.78 2.78 2.49 -6.18 -5.11 -6.15 3.49
Endosome -6.42 -3.45 3.07 8.00 5.48 -5.85 -2.24 0.65 6.22 2.43 -7.80 -4.53 2.61 9.69 5.84
Golgi 2.02 12.56 6.11 1.99 -6.69 -0.09 9.04 1.60 1.61 -7.61 -0.20 17.06 5.36 4.75 -11.75
Lipid Particles 1.41 -5.82 4.42 2.32 6.94 2.44 -0.26 6.01 4.39 2.60 2.96 -5.80 7.32 4.22 7.23
Mitochondria 7.44 22.24 13.26 1.32 -9.32 3.09 13.05 23.22 18.55 -2.93 5.73 27.13 25.29 15.63 -8.01
Mitotic Spindle -5.59 -10.88 -9.70 0.77 7.82 -3.82 -4.30 -11.01 -8.91 1.84 -6.60 -11.66 -14.66 -8.27 7.65
None -0.92 -3.27 0.67 1.19 3.39 -0.78 0.82 2.01 1.55 0.29 -0.96 -3.24 1.81 1.59 3.49
Nuclear Periphery -0.05 -2.84 0.97 1.21 2.91 0.61 -1.89 -0.97 -1.04 1.66 1.16 -3.28 -0.74 -1.06 3.12
Nuclear Periphery Foci -1.29 -5.55 -1.96 -1.67 1.63 1.52 -3.22 -0.19 -0.92 2.98 1.04 -6.14 -1.74 -2.10 3.49
Nucleolus -1.06 -4.45 1.24 1.39 4.63 1.22 -1.79 0.86 -0.13 1.87 0.07 -4.78 1.47 1.08 4.95
Nucleus -0.15 -6.27 0.33 0.37 6.31 0.89 -2.70 -0.63 -1.34 2.64 1.37 -6.83 -0.25 -1.37 6.79
Peroxisomes -3.30 -2.03 -8.94 -2.65 -5.44 -0.24 -5.09 -8.52 -7.98 2.58 -1.85 -4.29 -12.33 -9.34 -3.64
Vacuole -4.39 -11.19 -8.39 -1.57 7.24 -4.73 -4.52 -9.57 -7.61 3.21 -5.96 -12.10 -12.75 -8.63 8.03
Vacuole Periphery -0.46 -3.26 0.23 0.59 3.23 -0.93 -1.55 -1.23 -0.61 1.33 -0.04 -4.10 -0.76 -0.70 3.70