Standard name | |
---|---|
Human Ortholog | |
Description | Cell wall mannoprotein that localizes to birth scars of daughter cells; linked to a beta-1,3- and beta-1,6-glucan heteropolymer through a phosphodiester bond; required for propionic acid resistance |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 3 | 0 | 0 | 0 | 4 | 7 | – | 6 | 11 | 5 | 3 | 1 | 0 | 2 | 0 | 0 | 0 | 3 | 9 | 0 | 4 | 1 | 1 |
Bud | 0 | 6 | 0 | 0 | 0 | 8 | 5 | – | 1 | 6 | 6 | 8 | 0 | 0 | 1 | 0 | 0 | 0 | 22 | 62 | 3 | 7 | 22 | 20 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 1 | 1 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 1 | 0 | 1 | 1 | 2 | 12 | – | 17 | 21 | 7 | 0 | 5 | 1 | 5 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 201 | 415 | 79 | 44 | 11 | 50 | 96 | – | 85 | 85 | 70 | 70 | 174 | 168 | 260 | 193 | 132 | 121 | 48 | 126 | 38 | 98 | 79 | 61 |
Endoplasmic Reticulum | 44 | 60 | 10 | 5 | 3 | 15 | 41 | – | 27 | 40 | 22 | 12 | 23 | 14 | 16 | 16 | 16 | 5 | 128 | 167 | 18 | 28 | 48 | 28 |
Endosome | 10 | 13 | 2 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 3 | 5 | 7 | 8 | 2 | 7 | 4 | 10 | 19 | 1 | 8 | 9 | 20 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 3 | 1 | 0 | 3 | 2 | 6 |
Mitochondria | 4 | 0 | 0 | 1 | 0 | 23 | 9 | – | 19 | 25 | 24 | 29 | 0 | 0 | 1 | 10 | 2 | 5 | 8 | 10 | 4 | 14 | 7 | 5 |
Nucleus | 4 | 2 | 0 | 1 | 1 | 0 | 2 | – | 2 | 1 | 1 | 3 | 0 | 0 | 0 | 7 | 6 | 0 | 0 | 2 | 0 | 1 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 2 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 5 | 6 | 0 | 3 | 3 | 1 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 11 | 0 | 3 | 7 | 9 |
Vac/Vac Membrane | 75 | 122 | 11 | 1 | 3 | 7 | 36 | – | 9 | 12 | 14 | 7 | 9 | 10 | 20 | 54 | 61 | 26 | 101 | 186 | 35 | 47 | 105 | 127 |
Unique Cell Count | 305 | 568 | 96 | 50 | 18 | 88 | 183 | 140 | 164 | 126 | 117 | 207 | 193 | 296 | 246 | 188 | 146 | 341 | 617 | 105 | 228 | 297 | 298 | |
Labelled Cell Count | 345 | 623 | 104 | 53 | 19 | 109 | 211 | 168 | 205 | 152 | 139 | 221 | 201 | 315 | 284 | 226 | 162 | 341 | 617 | 105 | 228 | 297 | 298 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.8 | 8.6 | 6.2 | 5.0 | 9.4 | 5.6 | 9.1 | – | 8.6 | 9.3 | 9.1 | 7.8 | 8.2 | 6.3 | 7.1 | 6.9 | 9.0 | 6.1 | 6.2 | 6.8 | 7.5 |
Std Deviation (1e-4) | 3.6 | 4.4 | 2.6 | 0.9 | 3.1 | 2.1 | 5.0 | – | 3.5 | 4.4 | 4.2 | 4.0 | 4.8 | 3.1 | 3.6 | 2.7 | 18.5 | 1.9 | 5.7 | 5.1 | 6.6 |
Intensity Change (Log2) | – | – | – | -0.31 | 0.6 | -0.14 | 0.56 | – | 0.48 | 0.58 | 0.55 | 0.34 | 0.4 | 0.02 | 0.19 | 0.16 | 0.54 | -0.01 | -0.01 | 0.14 | 0.28 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 3.5 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.9 | -2.0 | -3.8 | -4.9 | – | -3.5 | -4.9 | -4.2 | -3.6 | 0.4 | 1.1 | 1.4 | -0.8 | -2.2 | 0.1 |
Endoplasmic Reticulum | -0.1 | 0 | 1.3 | 2.5 | – | 1.8 | 2.8 | 1.5 | 0 | 0.2 | -0.9 | -1.7 | -1.2 | -0.5 | -2.2 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.4 | 0 | – | 3.8 | 4.0 | 4.5 | 5.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | -0.8 | 1.7 | – | -1.4 | -1.1 | -0.1 | -1.4 | -2.3 | -1.9 | -1.5 | 2.2 | 3.8 | 1.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 15.9971 | 13.434 | 13.8277 | 14.0241 | 13.2617 | 15.2815 | 7.9623 | 7.7287 | 8.079 | 6.791 | 8.1621 | 9.7508 | 27.0126 | 23.9082 | 25.9618 | 24.7218 | 20.1996 | 24.3909 |
Actin | 0.004 | 0.0138 | 0.0044 | 0.0037 | 0.0048 | 0.0041 | 0.0062 | 0.0063 | 0.0103 | 0.0102 | 0.0016 | 0.0068 | 0.0015 | 0.0051 | 0.0052 | 0.0017 | 0.0064 | 0.0028 |
Bud | 0.0003 | 0.0141 | 0.0007 | 0.0008 | 0.0037 | 0.0004 | 0.0008 | 0.0069 | 0.0046 | 0.006 | 0.0256 | 0.0038 | 0.0002 | 0.0079 | 0.0031 | 0.0005 | 0.002 | 0.0009 |
Bud Neck | 0.0008 | 0.0004 | 0.0005 | 0.0004 | 0.0006 | 0.0007 | 0.0019 | 0.0018 | 0.0007 | 0.001 | 0.0034 | 0.0028 | 0.0002 | 0.0008 | 0.0004 | 0.0006 | 0.0005 | 0.0026 |
Bud Periphery | 0.0005 | 0.0259 | 0.0015 | 0.0012 | 0.0089 | 0.0023 | 0.0024 | 0.0114 | 0.0085 | 0.0189 | 0.0143 | 0.0146 | 0.0005 | 0.0214 | 0.0055 | 0.0011 | 0.0019 | 0.0049 |
Bud Site | 0.0016 | 0.0112 | 0.0048 | 0.0013 | 0.0006 | 0.0001 | 0.0101 | 0.0336 | 0.0089 | 0.0019 | 0.0089 | 0.0003 | 0.0008 | 0.0037 | 0.0051 | 0.0004 | 0.0003 | 0.0003 |
Cell Periphery | 0.0033 | 0.0104 | 0.0049 | 0.0042 | 0.0023 | 0.0014 | 0.0011 | 0.008 | 0.0065 | 0.0028 | 0.001 | 0.0013 | 0.0044 | 0.0145 | 0.008 | 0.0033 | 0.0009 | 0.0022 |
Cytoplasm | 0.0287 | 0.0831 | 0.0699 | 0.0789 | 0.1048 | 0.0687 | 0.1357 | 0.2268 | 0.2122 | 0.18 | 0.165 | 0.1946 | 0.066 | 0.0832 | 0.0318 | 0.0584 | 0.1253 | 0.0679 |
Cytoplasmic Foci | 0.0141 | 0.003 | 0.0053 | 0.0037 | 0.006 | 0.0025 | 0.0326 | 0.0108 | 0.0076 | 0.0066 | 0.0225 | 0.0079 | 0.0033 | 0.0008 | 0.0013 | 0.0013 | 0.0015 | 0.0028 |
Eisosomes | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0 | 0 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.7693 | 0.7174 | 0.8204 | 0.851 | 0.7876 | 0.8431 | 0.5042 | 0.4622 | 0.5319 | 0.5574 | 0.4518 | 0.6137 | 0.7971 | 0.7551 | 0.8571 | 0.8482 | 0.8251 | 0.8626 |
Endosome | 0.0127 | 0.0108 | 0.0084 | 0.004 | 0.0158 | 0.0067 | 0.0476 | 0.0202 | 0.0209 | 0.0167 | 0.0694 | 0.0168 | 0.0032 | 0.0029 | 0.0021 | 0.002 | 0.0062 | 0.0031 |
Golgi | 0.002 | 0.0013 | 0.0017 | 0.0009 | 0.0007 | 0.0005 | 0.0062 | 0.0013 | 0.0035 | 0.0015 | 0.0339 | 0.0046 | 0.0004 | 0.001 | 0.0064 | 0.0004 | 0.0005 | 0.0005 |
Lipid Particles | 0.0106 | 0.0116 | 0.0064 | 0.0068 | 0.0026 | 0.002 | 0.0213 | 0.0079 | 0.0067 | 0.0046 | 0.0044 | 0.0043 | 0.0054 | 0.005 | 0.0078 | 0.002 | 0.0015 | 0.0045 |
Mitochondria | 0.0044 | 0.0012 | 0.0004 | 0.0001 | 0.0013 | 0.0002 | 0.0071 | 0.0011 | 0.0032 | 0.001 | 0.0055 | 0.0031 | 0.0002 | 0.0006 | 0.0003 | 0.0003 | 0.0001 | 0.0001 |
None | 0.029 | 0.0223 | 0.024 | 0.0033 | 0.0113 | 0.0062 | 0.0511 | 0.0586 | 0.0609 | 0.0921 | 0.0965 | 0.0431 | 0.0223 | 0.011 | 0.0152 | 0.0211 | 0.0026 | 0.0032 |
Nuclear Periphery | 0.0809 | 0.0454 | 0.0218 | 0.0199 | 0.0257 | 0.0444 | 0.0776 | 0.0596 | 0.0473 | 0.0544 | 0.0245 | 0.0396 | 0.076 | 0.048 | 0.0303 | 0.0419 | 0.0159 | 0.0279 |
Nucleolus | 0.0042 | 0.0005 | 0.0004 | 0.0002 | 0.0006 | 0.0001 | 0.0019 | 0.0012 | 0.001 | 0.0006 | 0.0024 | 0.0012 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Nucleus | 0.0077 | 0.0077 | 0.0039 | 0.0016 | 0.008 | 0.0061 | 0.037 | 0.0196 | 0.0165 | 0.0109 | 0.0228 | 0.0106 | 0.0049 | 0.0102 | 0.0027 | 0.0043 | 0.0019 | 0.0041 |
Peroxisomes | 0.0044 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0113 | 0.0005 | 0.0019 | 0.0003 | 0.0027 | 0.0037 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Punctate Nuclear | 0.0039 | 0.0015 | 0.0026 | 0.0019 | 0.0018 | 0.0015 | 0.0178 | 0.003 | 0.0073 | 0.0041 | 0.0074 | 0.0025 | 0.0008 | 0.001 | 0.0007 | 0.0018 | 0.0012 | 0.001 |
Vacuole | 0.0133 | 0.0157 | 0.0164 | 0.0152 | 0.0109 | 0.0072 | 0.0206 | 0.0551 | 0.0361 | 0.0257 | 0.0332 | 0.0219 | 0.0095 | 0.0233 | 0.0149 | 0.0077 | 0.0055 | 0.0071 |
Vacuole Periphery | 0.0042 | 0.0023 | 0.0013 | 0.0008 | 0.002 | 0.0017 | 0.0052 | 0.0038 | 0.0033 | 0.0031 | 0.0031 | 0.0027 | 0.003 | 0.0036 | 0.0015 | 0.0026 | 0.0005 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 42.9102 | 139.7366 | 37.6133 | 32.0111 | 55.6349 | 27.5698 | 77.5696 | 55.159 | 40.7488 | 53.6925 |
Translational Efficiency | 1.4722 | 1.4356 | 1.5057 | 1.6489 | 1.6079 | 1.9826 | 1.7464 | 1.6484 | 1.3257 | 1.5435 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1364 | 1425 | 241 | 1728 | 151 | 2297 | 2495 | 1618 | 1515 | 3722 | 2736 | 3346 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 678.82 | 901.52 | 2483.13 | 1322.87 | 763.92 | 897.84 | 1939.57 | 1557.25 | 687.30 | 899.25 | 1987.45 | 1436.21 |
Standard Deviation | 100.04 | 197.73 | 953.22 | 549.29 | 117.41 | 221.52 | 835.36 | 584.53 | 105.04 | 212.73 | 860.30 | 578.58 |
Intensity Change Log 2 | — | 0.409330 | 1.871059 | 0.962570 | — | 0.233037 | 1.344243 | 1.027507 | — | 0.318673 | 1.616116 | 0.997318 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000324 | 0.000991 | 0.000335 | 0.000616 | 0.000311 | 0.000916 | 0.001452 | 0.000910 | 0.000323 | 0.000944 | 0.001354 | 0.000758 |
Bud Neck | 0.031058 | 0.017259 | 0.004230 | 0.012987 | 0.007879 | 0.015584 | 0.005761 | 0.007271 | 0.028747 | 0.016225 | 0.005626 | 0.010223 |
Bud Site | 0.018552 | 0.030249 | 0.004625 | 0.039297 | 0.005508 | 0.037916 | 0.032388 | 0.044269 | 0.017252 | 0.034980 | 0.029942 | 0.041702 |
Cell Periphery | 0.000540 | 0.001514 | 0.023890 | 0.001853 | 0.000970 | 0.001730 | 0.022262 | 0.010588 | 0.000583 | 0.001648 | 0.022405 | 0.006077 |
Cytoplasm | 0.431482* | 0.346656* | 0.007565 | 0.300466* | 0.405892* | 0.344133* | 0.072427 | 0.178704* | 0.428931* | 0.345099* | 0.066714 | 0.241586* |
Cytoplasmic Foci | 0.174633 | 0.132145 | 0.000843 | 0.019929 | 0.221304* | 0.090864 | 0.002383 | 0.012487 | 0.179285 | 0.106669 | 0.002247 | 0.016330 |
Eisosomes | 0.000378 | 0.000173 | 0.000108 | 0.000028 | 0.000271 | 0.000169 | 0.000077 | 0.000035 | 0.000368 | 0.000171 | 0.000080 | 0.000031 |
Endoplasmic Reticulum | 0.026915 | 0.200636* | 0.589058* | 0.062670 | 0.107497* | 0.192276* | 0.720787* | 0.208606* | 0.034947 | 0.195477* | 0.709183* | 0.133239* |
Endosome | 0.015016 | 0.028810 | 0.020065 | 0.025666 | 0.025771 | 0.029741 | 0.014496 | 0.024664 | 0.016088 | 0.029385 | 0.014987 | 0.025182 |
Golgi | 0.004119 | 0.005076 | 0.006076 | 0.002860 | 0.001532 | 0.005117 | 0.001597 | 0.004381 | 0.003861 | 0.005101 | 0.001991 | 0.003595 |
Lipid Particles | 0.015776 | 0.014738 | 0.009018 | 0.011839 | 0.021585 | 0.014674 | 0.007417 | 0.015226 | 0.016355 | 0.014698 | 0.007558 | 0.013477 |
Mitochondria | 0.007796 | 0.005634 | 0.000539 | 0.001971 | 0.004207 | 0.006657 | 0.003323 | 0.001933 | 0.007439 | 0.006265 | 0.003078 | 0.001953 |
Mitotic Spindle | 0.000410 | 0.005132 | 0.000307 | 0.018414 | 0.002315 | 0.004315 | 0.000426 | 0.005957 | 0.000600 | 0.004628 | 0.000415 | 0.012390 |
None | 0.004063 | 0.003257 | 0.000778 | 0.012212 | 0.001101 | 0.005845 | 0.006915 | 0.000992 | 0.003768 | 0.004854 | 0.006375 | 0.006787 |
Nuclear Periphery | 0.001541 | 0.002667 | 0.012805 | 0.008102 | 0.002345 | 0.004217 | 0.002873 | 0.001760 | 0.001621 | 0.003624 | 0.003747 | 0.005035 |
Nuclear Periphery Foci | 0.000848 | 0.005920 | 0.027383 | 0.033828 | 0.004945 | 0.004672 | 0.012392 | 0.017228 | 0.001256 | 0.005150 | 0.013713 | 0.025801 |
Nucleolus | 0.001024 | 0.000880 | 0.000053 | 0.001806 | 0.001407 | 0.001527 | 0.000131 | 0.000308 | 0.001062 | 0.001280 | 0.000124 | 0.001082 |
Nucleus | 0.154869* | 0.042903 | 0.002266 | 0.048213 | 0.108208 | 0.059791 | 0.010832 | 0.011869 | 0.150218* | 0.053325 | 0.010077 | 0.030639 |
Peroxisomes | 0.005917 | 0.004930 | 0.000010 | 0.000563 | 0.002889 | 0.003145 | 0.000110 | 0.000356 | 0.005615 | 0.003828 | 0.000101 | 0.000463 |
Vacuole | 0.103241 | 0.144726 | 0.254772* | 0.383013* | 0.072687 | 0.171440 | 0.078738 | 0.433292* | 0.100195 | 0.161212 | 0.094244 | 0.407326* |
Vacuole Periphery | 0.001500 | 0.005705 | 0.035274 | 0.013666 | 0.001376 | 0.005271 | 0.003214 | 0.019164 | 0.001488 | 0.005437 | 0.006038 | 0.016325 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.16 | -0.65 | -4.09 | 1.92 | -1.21 | -2.77 | -7.72 | -4.79 | -0.81 | 4.04 | -4.69 | -9.18 | -7.33 | 0.54 | 5.40 |
Bud Neck | 5.86 | 11.75 | 8.04 | 1.81 | -6.50 | -3.39 | 0.53 | -0.30 | 7.08 | -2.49 | 6.60 | 12.39 | 9.60 | 5.23 | -7.05 |
Bud Site | -3.58 | 3.84 | -6.07 | -2.35 | -9.41 | -10.69 | -10.46 | -12.36 | -2.68 | -3.02 | -6.73 | -5.74 | -9.49 | -3.14 | -3.17 |
Cell Periphery | -3.28 | -5.74 | -4.82 | -0.32 | 5.47 | -2.25 | -17.83 | -9.02 | -8.49 | 9.17 | -6.25 | -19.51 | -10.60 | -7.90 | 14.37 |
Cytoplasm | 7.96 | 57.24 | 11.56 | 3.17 | -39.67 | 2.80 | 15.41 | 9.07 | 15.06 | -17.11 | 10.01 | 47.63 | 19.86 | 11.86 | -31.38 |
Cytoplasmic Foci | 6.13 | 33.38 | 29.36 | 23.73 | -17.34 | 8.74 | 14.94 | 14.17 | 24.79 | -12.27 | 13.09 | 35.94 | 32.85 | 33.73 | -19.46 |
Eisosomes | 6.47 | 6.68 | 11.80 | 12.36 | 2.82 | 1.59 | 3.01 | 3.77 | 8.14 | 4.82 | 6.69 | 9.88 | 12.22 | 12.60 | 5.93 |
Endoplasmic Reticulum | -18.55 | -21.94 | -4.92 | 15.78 | 20.93 | -3.79 | -25.86 | -3.02 | 1.65 | 49.60* | -24.37 | -80.94 | -13.49 | 12.27 | 69.57* |
Endosome | -8.54 | -2.49 | -6.08 | 3.29 | -1.03 | -0.80 | 2.42 | 0.69 | 4.40 | -6.35 | -10.20 | 0.46 | -5.86 | 5.50 | -7.82 |
Golgi | -1.28 | -2.04 | 3.04 | 3.82 | 3.88 | -5.18 | -0.33 | -3.23 | 1.50 | -3.40 | -2.08 | 4.33 | 1.47 | 3.56 | -2.74 |
Lipid Particles | 0.74 | 3.07 | 2.81 | 2.23 | -0.85 | 1.69 | 3.34 | 1.59 | -0.15 | -4.71 | 1.40 | 6.84 | 2.44 | 1.48 | -4.62 |
Mitochondria | 2.19 | 8.99 | 8.46 | 5.06 | -1.98 | -1.13 | 0.17 | 1.00 | 5.82 | 3.28 | 1.46 | 5.81 | 8.01 | 7.99 | 4.77 |
Mitotic Spindle | -3.61 | 0.34 | -9.90 | -6.58 | -9.95 | -0.90 | 0.85 | -1.85 | -1.73 | -5.50 | -5.40 | 0.31 | -10.92 | -6.84 | -11.23 |
None | 1.14 | 3.86 | -3.48 | -4.20 | -5.60 | -6.21 | -7.49 | 0.21 | 6.32 | 7.60 | -1.39 | -2.87 | -1.78 | -0.74 | 0.83 |
Nuclear Periphery | -2.23 | -6.39 | -6.21 | -4.90 | 2.94 | -1.88 | -0.77 | 0.65 | 4.30 | 4.90 | -4.18 | -5.80 | -5.51 | -1.81 | -1.47 |
Nuclear Periphery Foci | -7.95 | -8.28 | -13.77 | -11.07 | -0.62 | 0.14 | -5.12 | -6.60 | -8.99 | -2.36 | -9.21 | -14.21 | -17.56 | -14.14 | -6.22 |
Nucleolus | 1.13 | 7.59 | -2.59 | -3.63 | -8.09 | -0.28 | 1.72 | 1.47 | 4.71 | -6.99 | -1.04 | 6.90 | 0.40 | 1.49 | -8.44 |
Nucleus | 23.74 | 33.51 | 20.02 | -3.83 | -18.45 | 4.82 | 9.57 | 9.16 | 18.15 | -3.08 | 22.42 | 33.57 | 26.58 | 8.24 | -15.23 |
Peroxisomes | 1.18 | 11.65 | 10.48 | 6.34 | -6.33 | -0.42 | 5.21 | 4.74 | 8.42 | -3.12 | 3.13 | 11.99 | 11.18 | 10.11 | -5.98 |
Vacuole | -6.49 | -8.25 | -31.75 | -25.72 | -5.86 | -12.42 | -2.01 | -34.70 | -27.80 | -39.67* | -11.98 | -0.42 | -45.27 | -37.04 | -46.05 |
Vacuole Periphery | -7.83 | -4.45 | -10.57 | -5.26 | 3.11 | -7.79 | -5.25 | -13.14 | -9.51 | -11.61 | -11.86 | -6.49 | -17.00 | -10.65 | -7.66 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|