Standard name
Description Cell wall mannoprotein that localizes to birth scars of daughter cells; linked to a beta-1,3- and beta-1,6-glucan heteropolymer through a phosphodiester bond; required for propionic acid resistance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0.05 0 – 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.09 0 – 0 0 0.05 0.07 0 0 0 0 0 0 0.07 0.1 0 0 0.08 0.07
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0.06 0 0.07 – 0.12 0.13 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.66 0.73 0.82 0.88 0.61 0.57 0.52 – 0.61 0.52 0.56 0.6 0.84 0.87 0.88 0.78 0.7 0.83 0.14 0.2 0.36 0.43 0.27 0.21
Endoplasmic Reticulum 0.14 0.11 0.1 0.1 0.17 0.17 0.22 – 0.19 0.24 0.17 0.1 0.11 0.07 0.05 0.07 0.09 0 0.38 0.27 0.18 0.13 0.16 0.1
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.26 0.05 – 0.14 0.15 0.19 0.25 0 0 0 0 0 0 0 0 0 0.06 0 0
Nucleus 0 0 0 0 0.06 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.25 0.21 0.11 0 0.17 0.08 0.2 – 0.06 0.07 0.11 0.06 0 0.05 0.07 0.22 0.32 0.18 0.3 0.3 0.33 0.21 0.35 0.43
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 3 0 0 0 4 7 – 6 11 5 3 1 0 2 0 0 0 3 9 0 4 1 1
Bud 0 6 0 0 0 8 5 – 1 6 6 8 0 0 1 0 0 0 22 62 3 7 22 20
Bud Neck 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 4 0 1 1 4
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 1 0 1 1 2 12 – 17 21 7 0 5 1 5 0 0 0 0 1 0 0 0 0
Cytoplasm 201 415 79 44 11 50 96 – 85 85 70 70 174 168 260 193 132 121 48 126 38 98 79 61
Endoplasmic Reticulum 44 60 10 5 3 15 41 – 27 40 22 12 23 14 16 16 16 5 128 167 18 28 48 28
Endosome 10 13 2 0 0 0 1 – 0 1 0 3 5 7 8 2 7 4 10 19 1 8 9 20
Golgi 0 0 0 0 0 0 1 – 0 1 1 0 1 0 0 1 0 1 3 1 0 3 2 6
Mitochondria 4 0 0 1 0 23 9 – 19 25 24 29 0 0 1 10 2 5 8 10 4 14 7 5
Nucleus 4 2 0 1 1 0 2 – 2 1 1 3 0 0 0 7 6 0 0 2 0 1 1 1
Nuclear Periphery 0 0 1 0 0 0 0 – 2 1 2 1 1 0 0 0 0 0 5 6 0 3 3 1
Nucleolus 1 0 0 0 0 0 0 – 0 1 0 1 0 0 0 1 1 0 0 0 0 0 0 3
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 1 – 0 0 0 1 1 1 1 0 1 0 1 11 0 3 7 9
Vac/Vac Membrane 75 122 11 1 3 7 36 – 9 12 14 7 9 10 20 54 61 26 101 186 35 47 105 127
Unique Cell Count 305 568 96 50 18 88 183 140 164 126 117 207 193 296 246 188 146 341 617 105 228 297 298
Labelled Cell Count 345 623 104 53 19 109 211 168 205 152 139 221 201 315 284 226 162 341 617 105 228 297 298


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.8 8.6 6.2 5.0 9.4 5.6 9.1 – 8.6 9.3 9.1 7.8 8.2 6.3 7.1 6.9 9.0 6.1 6.2 6.8 7.5
Std Deviation (1e-4) 3.6 4.4 2.6 0.9 3.1 2.1 5.0 – 3.5 4.4 4.2 4.0 4.8 3.1 3.6 2.7 18.5 1.9 5.7 5.1 6.6
Intensity Change (Log2) – – – -0.31 0.6 -0.14 0.56 – 0.48 0.58 0.55 0.34 0.4 0.02 0.19 0.16 0.54 -0.01 -0.01 0.14 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 3.5 3.7 0 0 0 0 0 0 0 0
Cytoplasm 0.9 -2.0 -3.8 -4.9 – -3.5 -4.9 -4.2 -3.6 0.4 1.1 1.4 -0.8 -2.2 0.1
Endoplasmic Reticulum -0.1 0 1.3 2.5 – 1.8 2.8 1.5 0 0.2 -0.9 -1.7 -1.2 -0.5 -2.2
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.4 0 – 3.8 4.0 4.5 5.2 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 -0.8 1.7 – -1.4 -1.1 -0.1 -1.4 -2.3 -1.9 -1.5 2.2 3.8 1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.9971 13.434 13.8277 14.0241 13.2617 15.2815 7.9623 7.7287 8.079 6.791 8.1621 9.7508 27.0126 23.9082 25.9618 24.7218 20.1996 24.3909
Actin 0.004 0.0138 0.0044 0.0037 0.0048 0.0041 0.0062 0.0063 0.0103 0.0102 0.0016 0.0068 0.0015 0.0051 0.0052 0.0017 0.0064 0.0028
Bud 0.0003 0.0141 0.0007 0.0008 0.0037 0.0004 0.0008 0.0069 0.0046 0.006 0.0256 0.0038 0.0002 0.0079 0.0031 0.0005 0.002 0.0009
Bud Neck 0.0008 0.0004 0.0005 0.0004 0.0006 0.0007 0.0019 0.0018 0.0007 0.001 0.0034 0.0028 0.0002 0.0008 0.0004 0.0006 0.0005 0.0026
Bud Periphery 0.0005 0.0259 0.0015 0.0012 0.0089 0.0023 0.0024 0.0114 0.0085 0.0189 0.0143 0.0146 0.0005 0.0214 0.0055 0.0011 0.0019 0.0049
Bud Site 0.0016 0.0112 0.0048 0.0013 0.0006 0.0001 0.0101 0.0336 0.0089 0.0019 0.0089 0.0003 0.0008 0.0037 0.0051 0.0004 0.0003 0.0003
Cell Periphery 0.0033 0.0104 0.0049 0.0042 0.0023 0.0014 0.0011 0.008 0.0065 0.0028 0.001 0.0013 0.0044 0.0145 0.008 0.0033 0.0009 0.0022
Cytoplasm 0.0287 0.0831 0.0699 0.0789 0.1048 0.0687 0.1357 0.2268 0.2122 0.18 0.165 0.1946 0.066 0.0832 0.0318 0.0584 0.1253 0.0679
Cytoplasmic Foci 0.0141 0.003 0.0053 0.0037 0.006 0.0025 0.0326 0.0108 0.0076 0.0066 0.0225 0.0079 0.0033 0.0008 0.0013 0.0013 0.0015 0.0028
Eisosomes 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0002 0.0002 0.0002 0.0003 0 0 0.0001 0.0004 0.0003 0.0001 0 0
Endoplasmic Reticulum 0.7693 0.7174 0.8204 0.851 0.7876 0.8431 0.5042 0.4622 0.5319 0.5574 0.4518 0.6137 0.7971 0.7551 0.8571 0.8482 0.8251 0.8626
Endosome 0.0127 0.0108 0.0084 0.004 0.0158 0.0067 0.0476 0.0202 0.0209 0.0167 0.0694 0.0168 0.0032 0.0029 0.0021 0.002 0.0062 0.0031
Golgi 0.002 0.0013 0.0017 0.0009 0.0007 0.0005 0.0062 0.0013 0.0035 0.0015 0.0339 0.0046 0.0004 0.001 0.0064 0.0004 0.0005 0.0005
Lipid Particles 0.0106 0.0116 0.0064 0.0068 0.0026 0.002 0.0213 0.0079 0.0067 0.0046 0.0044 0.0043 0.0054 0.005 0.0078 0.002 0.0015 0.0045
Mitochondria 0.0044 0.0012 0.0004 0.0001 0.0013 0.0002 0.0071 0.0011 0.0032 0.001 0.0055 0.0031 0.0002 0.0006 0.0003 0.0003 0.0001 0.0001
None 0.029 0.0223 0.024 0.0033 0.0113 0.0062 0.0511 0.0586 0.0609 0.0921 0.0965 0.0431 0.0223 0.011 0.0152 0.0211 0.0026 0.0032
Nuclear Periphery 0.0809 0.0454 0.0218 0.0199 0.0257 0.0444 0.0776 0.0596 0.0473 0.0544 0.0245 0.0396 0.076 0.048 0.0303 0.0419 0.0159 0.0279
Nucleolus 0.0042 0.0005 0.0004 0.0002 0.0006 0.0001 0.0019 0.0012 0.001 0.0006 0.0024 0.0012 0.0003 0.0004 0.0002 0.0002 0.0001 0.0001
Nucleus 0.0077 0.0077 0.0039 0.0016 0.008 0.0061 0.037 0.0196 0.0165 0.0109 0.0228 0.0106 0.0049 0.0102 0.0027 0.0043 0.0019 0.0041
Peroxisomes 0.0044 0.0001 0.0002 0.0001 0.0001 0.0001 0.0113 0.0005 0.0019 0.0003 0.0027 0.0037 0.0001 0 0.0001 0.0001 0 0
Punctate Nuclear 0.0039 0.0015 0.0026 0.0019 0.0018 0.0015 0.0178 0.003 0.0073 0.0041 0.0074 0.0025 0.0008 0.001 0.0007 0.0018 0.0012 0.001
Vacuole 0.0133 0.0157 0.0164 0.0152 0.0109 0.0072 0.0206 0.0551 0.0361 0.0257 0.0332 0.0219 0.0095 0.0233 0.0149 0.0077 0.0055 0.0071
Vacuole Periphery 0.0042 0.0023 0.0013 0.0008 0.002 0.0017 0.0052 0.0038 0.0033 0.0031 0.0031 0.0027 0.003 0.0036 0.0015 0.0026 0.0005 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.9102 139.7366 37.6133 32.0111 55.6349 27.5698 77.5696 55.159 40.7488 53.6925
Translational Efficiency 1.4722 1.4356 1.5057 1.6489 1.6079 1.9826 1.7464 1.6484 1.3257 1.5435

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘