Standard name
Description RNA polymerase II third largest subunit B44; part of central core; similar to prokaryotic alpha subunit

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0.07 0 0 0 0 0 0.05 0.05 0.05 0 0 0.10 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0
Nucleus 0.75 0.71 0.67 0.81 0.79 0.80 0.68 0.69 0.65 0.70 0.62 0.66 0.75 0.66 0.69 0.59 0.59 0.43 0.50 0.44 0.48
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.70 0.76 0.62 0.37 0.29 0.31 0.48 0.51 0.57 0.54 0.69 0.62 0.49 0.32 0.35 0.32 0.36 0.50 0.35 0.45 0.41
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 1 0 3 1 4
Bud Neck 0 1 0 0 0 0 1 1 1 2 1 0 1 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 4 0 8 4 6 1 0 2 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 2 4 2 10 6 8 11 12 14 10 5 6 19 16 1 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Endosome 0 1 0 0 1 1 1 2 0 0 0 0 1 3 2 1 0 0 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Mitochondria 0 1 0 0 1 1 9 3 12 7 16 5 1 0 0 0 0 0 0 2 1
Nucleus 15 119 41 107 181 173 212 182 164 197 135 134 131 123 150 33 104 41 38 121 110
Nuclear Periphery 0 0 0 0 0 0 2 1 1 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 14 127 38 49 66 66 152 135 142 150 151 126 86 60 75 18 62 48 27 124 93
Peroxisomes 0 1 0 0 0 0 1 0 0 0 0 0 0 0 4 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 2 1 0 0 0 0 1 5 4 0 1 0 1 0 1
Vac/Vac Membrane 0 4 1 2 6 1 0 1 1 1 1 1 1 3 7 0 4 0 3 15 12
Unique Cell Count 20 168 61 132 229 216 314 264 251 280 219 203 175 187 217 57 176 97 77 276 228
Labelled Cell Count 30 256 84 160 265 248 393 338 341 376 320 272 228 215 259 57 176 97 77 276 228

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.1 26.1 18.1 16.8 16.5 14.1 13.0 13.6 12.3 12.4 11.3 12.4 18.7 18.9 19.6 17.4 20.6 22.4
Std Deviation (1e-4) 4.8 6.2 5.5 4.3 4.0 3.6 3.7 3.6 3.7 4.0 3.2 3.5 5.8 6.5 7.1 6.2 5.7 7.2
Intensity Change (Log2) -0.1 -0.14 -0.36 -0.47 -0.42 -0.56 -0.55 -0.68 -0.54 0.05 0.06 0.11 -0.06 0.18 0.31

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 3.2 1.9 3.7 2.0 1.7 2.2 2.7 1.6 1.5 1.8 0.4 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -3.3 -4.8 -4.5 -2.0 -1.6 -0.8 -1.2 1.0 0 -1.8 -4.2 -3.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0