Standard name | |
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Human Ortholog | |
Description | RNA polymerase II third largest subunit B44; part of central core; similar to prokaryotic alpha subunit |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 3 | 1 | 4 |
Bud Neck | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 8 | 4 | 6 | 1 | 0 | 2 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 2 | 4 | 2 | 10 | 6 | 8 | 11 | 12 | 14 | 10 | 5 | 6 | 19 | 16 | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 |
Endosome | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | – | – | – | 1 | 0 | 0 | 0 | 1 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1 | 0 | 0 | 1 | 1 | 9 | 3 | 12 | 7 | 16 | 5 | 1 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 2 | 1 |
Nucleus | 15 | 119 | 41 | 107 | 181 | 173 | 212 | 182 | 164 | 197 | 135 | 134 | 131 | 123 | 150 | – | – | – | 33 | 104 | 41 | 38 | 121 | 110 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 14 | 127 | 38 | 49 | 66 | 66 | 152 | 135 | 142 | 150 | 151 | 126 | 86 | 60 | 75 | – | – | – | 18 | 62 | 48 | 27 | 124 | 93 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 5 | 4 | – | – | – | 0 | 1 | 0 | 1 | 0 | 1 |
Vac/Vac Membrane | 0 | 4 | 1 | 2 | 6 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 3 | 7 | – | – | – | 0 | 4 | 0 | 3 | 15 | 12 |
Unique Cell Count | 20 | 168 | 61 | 132 | 229 | 216 | 314 | 264 | 251 | 280 | 219 | 203 | 175 | 187 | 217 | 57 | 176 | 97 | 77 | 276 | 228 | |||
Labelled Cell Count | 30 | 256 | 84 | 160 | 265 | 248 | 393 | 338 | 341 | 376 | 320 | 272 | 228 | 215 | 259 | 57 | 176 | 97 | 77 | 276 | 228 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.1 | 26.1 | 18.1 | 16.8 | 16.5 | 14.1 | 13.0 | 13.6 | 12.3 | 12.4 | 11.3 | 12.4 | 18.7 | 18.9 | 19.6 | – | – | – | 17.4 | 20.6 | 22.4 |
Std Deviation (1e-4) | 4.8 | 6.2 | 5.5 | 4.3 | 4.0 | 3.6 | 3.7 | 3.6 | 3.7 | 4.0 | 3.2 | 3.5 | 5.8 | 6.5 | 7.1 | – | – | – | 6.2 | 5.7 | 7.2 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.14 | -0.36 | -0.47 | -0.42 | -0.56 | -0.55 | -0.68 | -0.54 | 0.05 | 0.06 | 0.11 | – | – | – | -0.06 | 0.18 | 0.31 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | 0 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleus | 2.1 | 1.9 | 2.1 | 0 | 0.3 | -0.3 | 0.5 | -0.8 | -0.2 | 1.2 | -0.2 | 0.3 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | -3.3 | -4.8 | -4.5 | -2.0 | -1.6 | -0.8 | -1.2 | 1.0 | 0 | -1.8 | -4.2 | -3.9 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 13.295 | 16.5946 | 14.7211 | 14.4105 | 13.7248 | 14.7298 | 15.2336 | 17.3373 | 16.4744 | 14.0084 | 12.7878 | 16.1865 | 15.0051 | 20.0802 | 19.4052 | 19.5747 | 17.7278 | 19.1686 |
Actin | 0.028 | 0 | 0.0202 | 0 | 0 | 0.0003 | 0.0452 | 0 | 0.0058 | 0.0525 | 0.0097 | 0.0049 | 0.0286 | 0 | 0.0117 | 0 | 0.0046 | 0.0015 |
Bud | 0.0007 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0.0003 | 0.0005 | 0.0002 | 0 | 0.0017 | 0 | 0.0006 | 0 | 0.0001 | 0.0001 |
Bud Neck | 0.0022 | 0 | 0.0082 | 0 | 0.0001 | 0.0003 | 0.0009 | 0.0001 | 0.0002 | 0.0015 | 0.0006 | 0.0002 | 0.0006 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 |
Bud Periphery | 0.0008 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0.0004 | 0.0011 | 0.0002 | 0.0001 | 0.0025 | 0 | 0.0009 | 0 | 0.0001 | 0.0001 |
Bud Site | 0.0031 | 0 | 0.0026 | 0 | 0.0002 | 0.0002 | 0.0027 | 0.0019 | 0.0006 | 0.0006 | 0.0004 | 0.0001 | 0.005 | 0 | 0.0017 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0005 | 0 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0112 | 0 | 0.0008 | 0 | 0 | 0.0001 | 0.0098 | 0.0006 | 0.0008 | 0.0002 | 0.0005 | 0 | 0.0021 | 0.0019 | 0.0015 | 0 | 0.0002 | 0.0001 |
Cytoplasmic Foci | 0.0147 | 0 | 0.0048 | 0 | 0.0001 | 0.0078 | 0.0314 | 0 | 0.0043 | 0.0012 | 0.012 | 0.0018 | 0.0157 | 0.0001 | 0.0043 | 0 | 0.0002 | 0.0001 |
Eisosomes | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0044 | 0 | 0.0006 | 0 | 0 | 0.0001 | 0.0025 | 0 | 0.0004 | 0.0006 | 0.0004 | 0 | 0.0041 | 0.0004 | 0.0013 | 0 | 0.0013 | 0.0003 |
Endosome | 0.057 | 0 | 0.0023 | 0 | 0 | 0.0018 | 0.0198 | 0 | 0.0069 | 0.0017 | 0.0108 | 0.0002 | 0.0111 | 0.0016 | 0.0139 | 0 | 0.0032 | 0.0022 |
Golgi | 0.0087 | 0 | 0.0027 | 0 | 0 | 0.0008 | 0.009 | 0 | 0.0015 | 0.0042 | 0.0052 | 0.0003 | 0.003 | 0 | 0.0103 | 0 | 0.0007 | 0.0006 |
Lipid Particles | 0.0256 | 0 | 0.0078 | 0 | 0.0001 | 0.005 | 0.0195 | 0 | 0.0068 | 0.0031 | 0.0254 | 0.0011 | 0.006 | 0 | 0.0034 | 0 | 0.0003 | 0.0002 |
Mitochondria | 0.0244 | 0.0001 | 0.0047 | 0.0311 | 0.0523 | 0.0017 | 0.0077 | 0.0001 | 0.0073 | 0.0232 | 0.0026 | 0.0008 | 0.0145 | 0.0002 | 0.0067 | 0 | 0.0036 | 0.0025 |
None | 0.0046 | 0 | 0.0005 | 0 | 0 | 0.0001 | 0.0047 | 0 | 0.0006 | 0.0002 | 0.0009 | 0.0002 | 0.0044 | 0.0002 | 0.0007 | 0 | 0.0002 | 0.0001 |
Nuclear Periphery | 0.0154 | 0.0006 | 0.0089 | 0.0001 | 0.0001 | 0.0036 | 0.008 | 0.0004 | 0.0012 | 0.0007 | 0.0033 | 0.0002 | 0.0451 | 0.0027 | 0.014 | 0 | 0.0527 | 0.0039 |
Nucleolus | 0.1628 | 0.0657 | 0.085 | 0.0592 | 0.5803 | 0.3429 | 0.1461 | 0.0688 | 0.0747 | 0.051 | 0.5713 | 0.307 | 0.1608 | 0.0786 | 0.0665 | 0.0395 | 0.506 | 0.299 |
Nucleus | 0.5829 | 0.9334 | 0.8402 | 0.9092 | 0.3649 | 0.6326 | 0.6604 | 0.9269 | 0.8833 | 0.8379 | 0.2943 | 0.6788 | 0.6586 | 0.9135 | 0.849 | 0.9604 | 0.4237 | 0.6882 |
Peroxisomes | 0.0143 | 0 | 0.0055 | 0 | 0.0001 | 0.0008 | 0.0069 | 0 | 0.0013 | 0.0177 | 0.0422 | 0.0005 | 0.0025 | 0 | 0.0038 | 0 | 0.0001 | 0 |
Punctate Nuclear | 0.0216 | 0.0001 | 0.001 | 0.0001 | 0.0005 | 0.0006 | 0.0188 | 0.0002 | 0.0012 | 0.0003 | 0.019 | 0.0033 | 0.0248 | 0.0001 | 0.0048 | 0 | 0.0011 | 0.0004 |
Vacuole | 0.0139 | 0 | 0.0011 | 0 | 0.0004 | 0.0005 | 0.0019 | 0.0003 | 0.0014 | 0.0005 | 0.0004 | 0.0001 | 0.0024 | 0.0004 | 0.0027 | 0 | 0.0002 | 0.0002 |
Vacuole Periphery | 0.0031 | 0 | 0.0018 | 0 | 0.0005 | 0.0005 | 0.0023 | 0.0001 | 0.0006 | 0.0007 | 0.0003 | 0.0001 | 0.0054 | 0.0003 | 0.0015 | 0 | 0.0013 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 40.9309 | 34.013 | 32.1225 | 49.9281 | 42.6044 | 33.418 | 39.6012 | 49.236 | 43.8749 | 48.7322 |
Translational Efficiency | 1.9364 | 2.7428 | 2.4088 | 1.5168 | 1.9551 | 2.4137 | 2.6404 | 1.7589 | 1.8058 | 1.9662 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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