TheCellVision

BAR1

Standard name	BAR1 ORF YIL015W Aliases aspartyl protease BAR1, SST1
Human Ortholog
Description	Aspartyl protease; secreted into the periplasmic space of mating type a cell; helps cells find mating partners; cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest

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Scale images

BAR1

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0
Bud	0	0	0	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0	0	0	0	0.05	0.05	0
Bud Neck	0	0	0	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0	0	0	0	0.05	0	0
Bud Site	0	0	0	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0
Cytoplasm	0.08	0.14	0.27	0.47	0.28	0.35	0.37	–	0.48	0.52	0.53	0.64	–	0.63	0.8	0.95	0.94	0.93	0	0	0.05	0	0	0.07
Endoplasmic Reticulum	0	0.05	0	0	0	0	0	–	0	0	0	0	–	0	0	0.07	0	0.06	0	0	0	0.4	0.38	0.4
Endosome	0	0	0.06	0	0	0	0	–	0	0	0	0	–	0.06	0	0	0	0	0	0	0	0	0.06	0
Golgi	0	0	0	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0
Mitochondria	0.08	0	0	0	0	0	0	–	0	0.05	0.06	0	–	0	0	0	0	0	0	0	0	0.11	0	0
Nucleus	0.2	0.11	0.18	0.16	0.14	0.17	0.23	–	0.19	0.21	0.1	0.09	–	0.07	0.09	0	0	0	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0
Vac/Vac Membrane	0.71	0.82	0.6	0.52	0.73	0.68	0.63	–	0.52	0.48	0.5	0.44	–	0.28	0.19	0	0	0	0.84	0.85	0.87	0.25	0.35	0.4

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	1	1	1	0	–	0	2	0	0	–	1	2	0	1	0	0	0	0	0	0	1
Bud	0	0	0	1	0	0	1	–	3	3	1	3	–	1	0	0	1	0	2	3	1	1	2	1
Bud Neck	0	0	0	0	0	0	0	–	0	0	0	1	–	0	1	2	1	4	0	0	0	1	0	1
Bud Site	0	0	0	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	–	0	0	1	0	–	0	1	3	4	1	0	0	0	0	0	0
Cytoplasm	11	22	24	102	77	100	153	–	190	216	177	241	–	77	138	283	251	172	1	3	5	0	0	8
Endoplasmic Reticulum	5	8	0	5	6	3	2	–	5	3	3	4	–	3	3	22	8	11	5	5	0	9	21	47
Endosome	5	5	5	6	6	8	2	–	2	3	1	0	–	7	2	3	2	3	6	6	2	0	3	2
Golgi	0	4	1	1	7	2	0	–	0	0	0	0	–	1	0	0	2	0	1	0	0	0	1	4
Mitochondria	11	0	1	1	1	6	3	–	9	19	20	12	–	0	0	7	5	2	1	1	0	2	0	0
Nucleus	27	16	16	36	40	48	94	–	76	86	34	35	–	9	16	3	2	1	1	0	0	0	2	0
Nuclear Periphery	1	0	0	0	0	0	0	–	2	0	0	1	–	0	0	0	0	0	1	0	0	0	0	0
Nucleolus	1	0	0	0	0	0	0	–	0	0	0	0	–	0	0	0	0	1	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	–	0	0	0	1	–	0	0	1	0	1	0	0	0	0	0	0
SpindlePole	1	0	0	2	0	0	0	–	1	0	0	1	–	1	1	0	1	0	1	0	0	0	0	0
Vac/Vac Membrane	96	124	53	114	205	193	263	–	209	201	165	167	–	34	33	9	11	8	125	139	98	5	20	46
Unique Cell Count	136	152	88	219	279	282	416		399	416	333	377		123	172	297	268	185	150	164	113	23	57	117
Labelled Cell Count	158	179	100	269	343	361	518		497	533	402	466		134	197	333	289	204	150	164	113	23	57	117

Yeast GFP Assignment

Vacuole

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	7.5	9.6	8.0	7.8	10.5	10.4	9.9	–	7.8	7.3	6.9	6.6	–	8.1	6.5	5.9	5.5	5.5	–	14.2	16.6
Std Deviation (1e^-4)	2.0	2.5	3.1	2.6	3.5	4.2	4.2	–	3.4	3.1	2.8	2.8	–	2.5	1.7	1.3	1.6	1.5	–	5.0	6.0
Intensity Change (Log₂)	–	–	–	-0.03	0.4	0.37	0.31	–	-0.03	-0.14	-0.22	-0.27	–	0.02	-0.3	-0.44	-0.54	-0.55	–	0.82	1.05

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Cortical Patches	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0
Bud	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0
Bud Neck	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0
Bud Site	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0
Cell Periphery	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0
Cytoplasm	3.1	0.1	1.4	1.7	–	3.5	4.2	4.3	6.3	–	5.1	8.3	13.9	12.9	11.3
Endoplasmic Reticulum	0	0	0	0	–	0	0	0	0	–	0	0	2.6	0	0
Endosome	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0
Golgi	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0
Mitochondria	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0
Nucleus	-0.4	-0.9	-0.3	0.9	–	0.2	0.5	-2.1	-2.4	–	-2.4	-2.1	0	0	-5.6
Nuclear Periphery	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0
Nucleolus	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0
Peroxisomes	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0
SpindlePole	0	0	0	0	–	0	0	0	0	–	0	0	0	0	0
Vacuole	-1.3	2.4	1.4	0.5	–	-1.3	-2.0	-1.8	-2.7	–	-4.7	-6.7	-12.8	-11.9	-10.4

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	1.8235	2.7905	1.732	0.8887	1.512	2.1013	2.0402	3.4024	1.896	1.0949	1.1185	2.7594	1.1444	2.3322	0.9927	0.7406	0.7383	1.7347
Actin	0.0019	0	0.0039	0.0002	0.0038	0.0003	0.0045	0	0.0114	0.0017	0	0.0021	0.0333	0.0002	0.0158	0.0018	0.0002	0.0044
Bud	0.0008	0.0016	0.0025	0.0187	0.0187	0.0035	0.0004	0.0008	0.0007	0.0005	0.0004	0.0005	0.0063	0.015	0.0051	0.0005	0.0011	0.0005
Bud Neck	0.0014	0.0014	0.0031	0.0021	0.0015	0.0028	0.0009	0.001	0.0027	0.0022	0.001	0.0038	0.0039	0.0017	0.0013	0.0029	0.0018	0.0027
Bud Periphery	0.0017	0.0037	0.0044	0.0232	0.0113	0.007	0.0014	0.0013	0.0017	0.0012	0.0006	0.0017	0.0019	0.0072	0.0046	0.0009	0.0042	0.0013
Bud Site	0.0025	0.0076	0.0061	0.0084	0.0029	0.0012	0.0013	0.0024	0.0024	0.0022	0.0009	0.001	0.0041	0.0275	0.01	0.0064	0.0013	0.001
Cell Periphery	0.0031	0.0058	0.004	0.002	0.0024	0.0042	0.0029	0.0075	0.0034	0.0014	0.0015	0.0037	0.002	0.0039	0.0027	0.0021	0.0053	0.0044
Cytoplasm	0.0104	0.0135	0.012	0.0069	0.0159	0.0209	0.0271	0.0183	0.0142	0.0107	0.0034	0.0075	0.0126	0.0263	0.0157	0.005	0.0099	0.016
Cytoplasmic Foci	0.0306	0.0148	0.0294	0.0554	0.0249	0.0265	0.0295	0.009	0.0326	0.012	0.0222	0.0181	0.0421	0.0146	0.0342	0.0195	0.0132	0.0249
Eisosomes	0.0002	0	0.0001	0	0.0001	0	0.0003	0	0.0001	0.0006	0	0	0.0003	0	0.0003	0	0	0
Endoplasmic Reticulum	0.0082	0.0062	0.0033	0.0018	0.0024	0.0042	0.0072	0.008	0.0031	0.0014	0.0018	0.0041	0.0058	0.0071	0.0047	0.0008	0.0014	0.0028
Endosome	0.2762	0.1443	0.2113	0.23	0.1944	0.2285	0.2319	0.1308	0.2306	0.1931	0.3781	0.1395	0.2458	0.1682	0.2224	0.153	0.1542	0.1651
Golgi	0.0129	0.0042	0.0076	0.0044	0.0167	0.0066	0.0154	0.0036	0.016	0.0125	0.0119	0.0059	0.015	0.005	0.0092	0.0044	0.0018	0.0045
Lipid Particles	0.0409	0.013	0.03	0.0289	0.025	0.0245	0.0586	0.0184	0.0293	0.0198	0.0154	0.0159	0.0481	0.0183	0.0303	0.0062	0.005	0.0149
Mitochondria	0.036	0.0175	0.0635	0.0043	0.0226	0.0244	0.0226	0.0082	0.0227	0.0068	0.0039	0.0119	0.0335	0.0121	0.0206	0.002	0.0027	0.0062
None	0.0333	0.0278	0.055	0.0236	0.0623	0.0199	0.0578	0.0164	0.0329	0.0744	0.0009	0.001	0.0329	0.0179	0.0589	0.0746	0.0025	0.013
Nuclear Periphery	0.012	0.0076	0.0065	0.0047	0.0043	0.0046	0.014	0.0041	0.0038	0.0022	0.0044	0.0047	0.0067	0.007	0.0105	0.0015	0.0016	0.0039
Nucleolus	0.0094	0.0071	0.0135	0.011	0.0054	0.004	0.0021	0.0037	0.0059	0.0037	0.004	0.003	0.0035	0.0047	0.0059	0.0076	0.0043	0.0026
Nucleus	0.0137	0.0092	0.0154	0.0193	0.0089	0.0065	0.0047	0.0041	0.0036	0.0055	0.002	0.0049	0.0046	0.0058	0.0057	0.0037	0.0044	0.0037
Peroxisomes	0.0076	0.0009	0.0064	0.0036	0.0069	0.0022	0.0167	0.001	0.0102	0.0071	0.0017	0.0074	0.0126	0.0019	0.0076	0.0098	0.0006	0.0015
Punctate Nuclear	0.0042	0.001	0.0044	0.005	0.0196	0.0008	0.0057	0.0002	0.0011	0.0044	0.0002	0.001	0.0066	0.0005	0.0015	0.0006	0.0002	0.0007
Vacuole	0.3754	0.5997	0.3969	0.4372	0.4216	0.5162	0.4121	0.6669	0.4478	0.5423	0.4117	0.6494	0.4056	0.5515	0.4339	0.613	0.7006	0.6146
Vacuole Periphery	0.1175	0.113	0.1207	0.1093	0.1284	0.0911	0.0826	0.0943	0.1237	0.0942	0.1342	0.1133	0.0728	0.1036	0.0989	0.0835	0.0838	0.1116

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	15.9506	12.3017	31.5346	148.0505	69.3409	23.7563	17.668	21.1783	71.7075	75.1904
Translational Efficiency	0.9383	0.7067	1.181	0.7941	0.9564	0.6987	0.7799	0.6298	1.0839	0.9178

Hit Data

Dataset	Hit
Protein Concentration	✔
Protein Localization	✘
Gene Expression	✔
Translational Efficiency	✘

Proteome Dynamics

Micrographs

Cell Count

R1				R2				R1 & R2
WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
846	922	1254	1984	1093	2026	1636	664	1939	2948	2890	2648

Protein Abundance

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Mean	573.27	846.03	1066.37	813.03	740.88	965.46	903.88	801.93	667.75	928.11	974.39	810.25
Standard Deviation	102.91	164.52	176.97	110.67	126.47	196.87	133.96	122.45	143.34	195.36	173.88	113.84
Intensity Change Log ₂	—	0.561494	0.895421	0.504094	—	0.381977	0.286891	0.114236	—	0.463047	0.584249	0.297368

Localization Score (DeepLoc)

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Actin	0.000153	0.000243	0.001821	0.001221	0.000060	0.000066	0.001214	0.000690	0.000100	0.000121	0.001477	0.001088
Bud Neck	0.027164	0.016688	0.018304	0.011824	0.027520	0.008814	0.016115	0.004116	0.027365	0.011277	0.017065	0.009892
Bud Site	0.012010	0.042812	0.028440	0.049853	0.021352	0.011846	0.010901	0.028703	0.017276	0.021531	0.018511	0.044550
Cell Periphery	0.002977	0.001487	0.003534	0.000353	0.001643	0.009107	0.001319	0.000148	0.002225	0.006724	0.002280	0.000302
Cytoplasm	0.422573*	0.109420*	0.238445*	0.447646*	0.222169*	0.008574	0.412590*	0.462315*	0.309606*	0.040114	0.337027*	0.451325*
Cytoplasmic Foci	0.110235	0.034393	0.039189	0.018793	0.084331	0.002957	0.041379	0.035049	0.095633	0.012788	0.040429	0.022870
Eisosomes	0.000293	0.000027	0.000076	0.000056	0.000084	0.000029	0.000168	0.000060	0.000175	0.000028	0.000128	0.000057
Endoplasmic Reticulum	0.002488	0.003775	0.040770	0.002890	0.002740	0.018123	0.027364	0.002138	0.002630	0.013636	0.033181	0.002701
Endosome	0.009692	0.086087	0.037544	0.005372	0.056085	0.048917	0.017481	0.010200	0.035844	0.060542	0.026187	0.006583
Golgi	0.000922	0.006392	0.001595	0.002123	0.001214	0.001801	0.000536	0.013151	0.001087	0.003237	0.000995	0.004889
Lipid Particles	0.010393	0.001127	0.010550	0.001139	0.003614	0.001419	0.011633	0.001650	0.006572	0.001328	0.011163	0.001267
Mitochondria	0.003638	0.002078	0.000382	0.002707	0.002331	0.002334	0.000301	0.002322	0.002901	0.002254	0.000336	0.002611
Mitotic Spindle	0.000145	0.001005	0.001191	0.033709	0.000427	0.000243	0.002014	0.016740	0.000304	0.000482	0.001657	0.029454
None	0.014873	0.000730	0.000593	0.003911	0.002217	0.000256	0.002576	0.006347	0.007739	0.000404	0.001716	0.004522
Nuclear Periphery	0.001028	0.000133	0.000838	0.001609	0.000400	0.000424	0.001380	0.001545	0.000674	0.000333	0.001145	0.001593
Nuclear Periphery Foci	0.000312	0.000424	0.010138	0.000952	0.000419	0.000564	0.006670	0.002330	0.000372	0.000520	0.008175	0.001297
Nucleolus	0.001967	0.000945	0.000588	0.000600	0.001178	0.001147	0.000448	0.000286	0.001522	0.001084	0.000508	0.000521
Nucleus	0.209583*	0.010587	0.047803	0.308580*	0.050457	0.003638	0.167874*	0.277409*	0.119885	0.005812	0.115774	0.300764*
Peroxisomes	0.002163	0.002927	0.001481	0.001305	0.001968	0.000396	0.001501	0.000929	0.002053	0.001187	0.001492	0.001211
Vacuole	0.166242	0.630844*	0.513218*	0.100469	0.503523*	0.840618*	0.276112*	0.129435	0.356365*	0.775010*	0.378994*	0.107733
Vacuole Periphery	0.001148	0.047878	0.003502	0.004885	0.016269	0.038728	0.000425	0.004438	0.009672	0.041590	0.001760	0.004773

Localization Changes (T-score)

	R1					R2					R1 & R2
	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14
Actin	-0.86	-5.79	-3.13	-2.48	2.85	-0.42	-4.74	-4.94	-4.83	2.37	-0.55	-7.44	-4.14	-3.96	2.80
Bud Neck	3.30	3.23	6.41	4.57	5.07	7.85	5.26	9.85	4.41	6.54	8.55	6.07	10.62	3.59	6.58
Bud Site	-8.25	-5.55	-4.67	4.37	0.62	3.92	5.19	1.33	-1.28	-2.08	-2.23	0.11	-2.43	-0.61	-2.74
Cell Periphery	1.48	0.06	2.81	4.25	3.41	-8.95	1.73	6.36	11.19	2.94	-6.30	0.69	4.52	11.59	4.28
Cytoplasm	25.16	14.00	6.12	-25.12	-10.70	24.96	-15.53	-8.80	-31.10	4.06	36.86	-2.75	-4.71	-53.05	-2.23
Cytoplasmic Foci	13.55	13.80	19.97	7.72	11.93	18.37	10.23	12.28	-10.89	4.61	24.32	16.68	23.68	-2.56	14.61
Eisosomes	7.04	5.78	6.19	-7.90	1.39	11.38	-3.93	1.00	-14.04	4.45	8.79	3.41	6.55	-12.58	5.27
Endoplasmic Reticulum	-1.11	-9.95	-1.16	0.35	9.94	-12.78	-8.12	-1.52	12.52	7.91	-11.83	-12.79	-1.84	11.86	12.60
Endosome	-15.70	-11.33	7.82	17.36	15.67	1.75	12.28	15.03	18.00	6.45	-8.46	5.99	16.51	26.11	17.67
Golgi	-4.71	-1.68	0.37	4.69	1.49	-2.15	4.77	-1.92	-1.58	-2.38	-5.26	1.75	-1.25	2.28	-1.77
Lipid Particles	8.29	0.33	8.24	-0.80	9.58	4.25	-5.37	5.62	-0.98	7.19	8.80	-3.51	9.97	-0.30	11.57
Mitochondria	1.86	4.92	2.90	0.79	-4.21	-0.02	4.19	2.13	1.60	-2.70	0.99	6.45	3.19	1.43	-5.12
Mitotic Spindle	-2.40	-4.69	-9.59	-9.05	-8.88	0.81	-2.87	-4.18	-4.24	-3.45	-1.10	-4.05	-10.36	-10.25	-9.11
None	6.28	6.34	4.77	-13.09	-14.34	4.67	-0.22	-3.56	-7.13	-3.48	7.19	5.92	3.29	-16.66	-8.80
Nuclear Periphery	3.05	0.14	-6.58	-27.43	-15.57	-0.14	-9.94	-11.67	-10.16	-4.13	2.04	-4.75	-13.61	-20.27	-14.15
Nuclear Periphery Foci	-0.95	-12.93	-5.51	-4.59	12.02	-0.82	-10.62	-3.18	-2.70	6.28	-1.17	-16.65	-6.10	-4.03	14.33
Nucleolus	1.87	2.86	3.13	1.92	0.69	0.10	5.85	6.67	3.17	1.82	1.50	4.58	4.80	3.25	0.54
Nucleus	24.18	17.22	-15.95	-56.95*	-44.60*	14.24	-22.86	-24.54	-30.45	-9.80	25.95	-3.07	-34.53	-65.98	-33.98
Peroxisomes	-1.54	3.09	6.12	4.62	3.46	5.63	2.47	4.21	-0.88	2.45	3.51	3.75	6.77	2.97	3.99
Vacuole	-31.82*	-26.12	-1.71	37.59*	32.58*	-27.84	16.55	15.64	50.05*	2.16	-42.39*	-2.31	15.41	83.60*	23.87
Vacuole Periphery	-16.46	-5.00	-1.20	15.97	1.95	-10.88	10.98	9.16	22.54	-1.89	-19.98	9.60	8.47	27.94	-0.53