Standard name
Description Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C16:0-C18:0 chain lengths; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.14 0.05 0 0 0.09 0 0.08 0.06 0.06 0 0 0.05 0.14 0.12 0.12 0.17 0.17 0.12 0.06 0.05 0.06 0.06 0.08 0.05
Bud 0.06 0.05 0 0 0 0 0 0 0.05 0.05 0.07 0.05 0.09 0 0 0 0 0 0 0.05 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0.06 0 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.46 0.39 0.36 0.34 0.32 0.2 0.15 0.08 0.14 0.11 0.09 0.1 0.59 0.51 0.49 0.63 0.61 0.69 0.52 0.35 0.42 0.52 0.63 0.59
Cytoplasm 0.61 0.75 0.79 0.8 0.71 0.81 0.8 0.81 0.77 0.78 0.74 0.74 0.58 0.65 0.66 0.32 0.29 0.27 0.19 0.28 0.25 0.16 0.07 0.13
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0.09 0.07 0 0.05 0 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.1 0.07 0.19 0.17 0.22 0.19 0 0 0 0.07 0 0 0 0 0.07 0.05 0.06 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 32 12 16 15 41 22 48 32 34 22 19 23 29 41 50 71 44 34 15 14 24 11 21 20
Bud 15 11 2 6 13 15 26 22 27 23 35 27 18 10 15 8 11 10 3 14 16 8 11 15
Bud Neck 0 2 13 9 18 10 20 16 11 13 4 8 0 1 0 0 1 0 9 24 24 12 12 18
Bud Site 1 0 0 0 2 1 1 0 2 1 0 1 0 1 0 0 0 0
Cell Periphery 107 86 135 124 141 99 92 40 74 54 45 51 124 180 214 268 161 190 129 93 176 103 181 229
Cytoplasm 141 165 298 290 316 405 482 426 406 382 360 369 122 229 286 135 78 75 46 73 105 32 19 51
Endoplasmic Reticulum 4 6 6 1 3 7 5 8 4 4 0 4 1 1 4 20 3 19 23 18 10 10 12 22
Endosome 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 2 2 3 0 0 1
Golgi 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 2 3 2 1 0 2 2 1 0
Mitochondria 3 1 1 11 9 44 63 37 99 83 108 95 0 1 0 28 9 10 9 9 30 9 16 14
Nucleus 2 1 0 0 0 0 0 4 1 1 7 1 0 1 1 3 3 5 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 3 2 0 1 2 0 0 0 1 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 3 0 3 2 7 6 5 9 4 12 2 12 0 1 3 8 3 10 0 0 2 1 1 2
Vac/Vac Membrane 0 1 1 0 0 2 4 11 2 8 13 10 1 0 0 8 6 1 4 7 15 4 6 8
Unique Cell Count 232 219 376 363 442 497 604 529 524 491 488 499 209 352 434 428 266 277 251 264 424 201 288 387
Labelled Cell Count 308 285 476 458 552 611 749 607 664 605 596 601 295 467 577 551 322 356 251 264 424 201 288 387


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 6.4 6.1 5.7 6.1 4.8 4.9 5.2 4.3 4.5 4.3 4.8 7.0 7.0 7.2 10.8 10.6 10.7 6.2 7.0 6.8
Std Deviation (1e-4) 2.4 2.2 1.5 1.7 1.5 1.3 1.3 1.3 1.2 1.4 1.4 1.3 2.1 2.3 2.3 2.2 2.2 2.1 2.1 2.3 2.1
Intensity Change (Log2) -0.1 0.01 -0.35 -0.31 -0.24 -0.52 -0.46 -0.5 -0.35 0.19 0.2 0.24 0.82 0.8 0.81 0.01 0.19 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.1 2.8 0 2.3 1.2 0 0 0 0 4.2 3.7 3.8 5.6 5.3 3.8
Bud 0 2.6 0 3.4 3.3 0 0 0 0 5.2 2.4 2.9 0 3.2 2.9
Bud Neck -0.8 0.5 0 -0.1 -0.4 0 0 0 0 0 -3.1 -3.9 -3.9 -2.6 -3.1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.5 -1.2 0 -7.5 -10.6 0 0 0 0 5.5 4.1 3.8 7.6 6.2 8.3
Cytoplasm 0.2 -2.6 0.8 0.2 0.5 -0.6 -0.5 -1.9 -1.8 -5.4 -4.3 -4.2 -13.5 -12.7 -13.3
Endoplasmic Reticulum 0 0 0 0 -0.1 0 0 0 0 0 0 0 2.5 0 3.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.0 0 0 6.3 5.0 0 0 0 0 0 0 0 4.8 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1.3 0 2.5
Vacuole 0 0 0 0 0 0 0 2.8 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Faa3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available