Standard name
Human Ortholog
Description ER protein with chaperone and co-chaperone activity; involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.12 0.13 0.3 0.29 0.37 0.49 0.52 0.54 0.57 0.64 0.62 0.59 0.62 0.82 0.35 0.29 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07 0 0 0 0 0 0
Endosome 0 0 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0.05 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.23 0.22 0.06 0.06 0.12 0.08 0.09 0.14 0.16 0.15 0.13 0 0 0 0.08 0.09 0.06 0 0 0 0 0 0
Nucleus 0.21 0.11 0.07 0.07 0 0.06 0.06 0.06 0 0.05 0 0.05 0 0 0 0.42 0.36 0.31 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.78 0.8 0.76 0.81 0.8 0.73 0.71 0.62 0.57 0.54 0.45 0.47 0.45 0.39 0.19 0.44 0.47 0.64 0.87 0.9 0.89 0.87 0.84 0.8
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 1 0 1
Bud 0 0 1 0 0 0 0 0 0 1 1 1 2 2 1 0 0 1 0 1 0 1 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 2 2 1 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 30 36 29 54 79 108 211 266 201 183 233 264 113 113 80 74 51 25 0 2 0 1 7 6
Endoplasmic Reticulum 0 0 0 0 1 0 4 1 0 1 0 2 3 0 0 10 11 9 0 0 0 0 3 2
Endosome 5 0 7 10 16 7 7 2 1 0 0 1 7 6 1 4 6 7 16 12 7 12 19 27
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 1 1 1 2 0 3
Mitochondria 27 67 47 10 16 35 33 44 52 52 55 55 0 0 0 17 15 8 12 6 8 5 12 15
Nucleus 70 32 15 13 9 17 26 32 16 16 12 22 5 5 3 87 62 43 7 2 0 1 4 2
Nuclear Periphery 2 2 0 0 1 0 4 5 8 3 2 6 0 0 0 2 0 0 6 0 0 1 4 2
Nucleolus 0 0 0 0 0 1 0 0 0 1 1 1 0 0 0 0 0 1 0 0 0 1 1 0
Peroxisomes 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 2 3 0 0 0 0 0 1 1 0 0 0 0 0 1 0 1 2 2
Vac/Vac Membrane 260 231 164 146 221 213 309 317 212 172 164 203 85 72 19 93 81 89 320 275 206 208 328 293
Unique Cell Count 333 290 217 180 275 292 434 514 371 321 362 428 191 183 98 209 173 138 369 305 231 239 391 367
Labelled Cell Count 395 368 263 233 345 383 597 667 490 429 468 556 218 202 106 288 226 186 369 305 231 239 391 367


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 5.6 5.7 6.4 6.8 5.7 5.6 5.6 5.1 4.8 4.9 5.1 6.2 6.2 5.9 5.1 5.8 6.8 7.0 6.7 7.1
Std Deviation (1e-4) 1.3 1.2 1.6 1.8 1.7 1.6 1.5 1.6 1.3 1.1 1.4 1.2 1.4 1.4 1.7 1.1 1.4 1.6 1.7 1.5 1.9
Intensity Change (Log2) 0.18 0.26 0.01 -0.01 -0.02 -0.15 -0.24 -0.2 -0.16 0.13 0.13 0.06 -0.15 0.03 0.27 0.3 0.25 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4.1 4.1 5.9 8.8 9.7 9.8 10.2 11.9 11.6 9.7 10.1 11.8 5.3 3.9 1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 1.1 1.4 -0.6 0 0 0 0 0 0 0.2 0 0 -0.9 0.1 0.9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -4.6 -5.2 -2.9 -5.1 -4.9 -2.4 0 -2.0 -2.9 -6.8 -6.7 -5.0 -3.9 -3.5 -4.0
Nucleus 0.1 -1.9 -0.5 -0.5 -0.3 -1.4 -0.9 -2.0 -0.9 -2.0 -1.9 -1.4 8.4 7.1 6.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.3 1.3 -0.7 -1.2 -3.6 -4.5 -5.2 -7.1 -6.8 -6.4 -7.3 -9.4 -6.6 -5.8 -2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6476 2.7373 2.0418 1.9911 1.6676 2.2762 2.4594 3.6835 3.1717 2.9013 3.1141 3.4375 2.6661 3.7614 3.1885 3.5936 2.9123 3.4412
Actin 0.0169 0.0053 0.0098 0 0.0036 0.0007 0.0188 0 0.0021 0.0086 0.0561 0.0004 0.0095 0 0.0056 0 0.0102 0.0002
Bud 0.0006 0.0005 0.0005 0.0003 0.0006 0.0018 0.0031 0.0003 0.0005 0.0008 0.0009 0.0003 0.0004 0.0003 0.0005 0.0002 0.0004 0.0007
Bud Neck 0.0016 0.0011 0.002 0.0007 0.0012 0.0008 0.0011 0.0008 0.0009 0.0106 0.0012 0.001 0.0016 0.0014 0.001 0.0004 0.0009 0.0012
Bud Periphery 0.0013 0.0008 0.0009 0.0006 0.0009 0.0009 0.0036 0.0005 0.0012 0.0015 0.0021 0.0006 0.0009 0.0006 0.001 0.0003 0.0008 0.0015
Bud Site 0.0021 0.0019 0.0021 0.0009 0.0016 0.0005 0.0053 0.0015 0.0014 0.0073 0.0019 0.0006 0.0016 0.001 0.001 0.0005 0.0016 0.0014
Cell Periphery 0.0011 0.0009 0.001 0.0006 0.0008 0.001 0.0009 0.0012 0.0012 0.0026 0.0006 0.0009 0.0017 0.0132 0.0011 0.0006 0.0006 0.0011
Cytoplasm 0.0161 0.0253 0.0106 0.0048 0.01 0.0118 0.0091 0.0194 0.0161 0.0038 0.0031 0.0062 0.0126 0.0148 0.0164 0.0037 0.0165 0.0141
Cytoplasmic Foci 0.0186 0.0086 0.0236 0.0086 0.0156 0.0076 0.0171 0.0168 0.0148 0.0204 0.0391 0.01 0.0142 0.011 0.0143 0.0062 0.014 0.0078
Eisosomes 0.0001 0 0.0001 0 0.0001 0 0.0002 0 0.0002 0.0002 0.0004 0 0.0008 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0057 0.0032 0.0037 0.0016 0.0052 0.0031 0.0043 0.006 0.0069 0.002 0.0048 0.0044 0.0053 0.0078 0.0062 0.0011 0.0035 0.0057
Endosome 0.3194 0.2078 0.3173 0.3336 0.334 0.2216 0.3369 0.2589 0.3461 0.3719 0.381 0.3074 0.3343 0.2175 0.3149 0.3079 0.2662 0.2709
Golgi 0.014 0.0049 0.0157 0.009 0.0183 0.0103 0.0172 0.0058 0.0195 0.0254 0.0203 0.0118 0.0109 0.0055 0.01 0.0048 0.0083 0.007
Lipid Particles 0.0104 0.0062 0.0156 0.0067 0.0103 0.0205 0.0104 0.0074 0.0056 0.0192 0.0183 0.0059 0.0197 0.0036 0.0049 0.0038 0.0123 0.0118
Mitochondria 0.0218 0.008 0.0175 0.0325 0.0081 0.0184 0.038 0.004 0.0291 0.0136 0.021 0.0102 0.0298 0.0041 0.0284 0.002 0.0071 0.0052
None 0.014 0.0043 0.013 0.0002 0.0019 0.0035 0.0042 0.0045 0.023 0.0011 0.0004 0.0028 0.0095 0.001 0.0175 0.0001 0.0027 0.0004
Nuclear Periphery 0.0252 0.0076 0.0071 0.0031 0.0168 0.0061 0.0115 0.0238 0.0095 0.0025 0.0038 0.0068 0.0052 0.0082 0.0073 0.0025 0.0128 0.0063
Nucleolus 0.0051 0.0014 0.0029 0.0018 0.0015 0.0013 0.0012 0.0022 0.002 0.0054 0.0008 0.001 0.0062 0.0009 0.0012 0.0007 0.0015 0.0016
Nucleus 0.0084 0.0037 0.0035 0.0018 0.0063 0.0029 0.0036 0.0038 0.0064 0.0022 0.002 0.0018 0.0059 0.0056 0.0024 0.0016 0.0049 0.0075
Peroxisomes 0.0065 0.0007 0.0038 0.0009 0.0026 0.0026 0.0045 0.0005 0.001 0.0048 0.0729 0.0009 0.0023 0.0007 0.0011 0.0003 0.0112 0.0029
Punctate Nuclear 0.0011 0.0003 0.0009 0.0001 0.0009 0.0004 0.001 0.0002 0.0003 0.0002 0.0004 0.0001 0.0013 0.0002 0.0002 0 0.0013 0.0001
Vacuole 0.3878 0.5646 0.4311 0.4862 0.4708 0.5454 0.3953 0.4898 0.3881 0.4013 0.2996 0.4569 0.4152 0.5802 0.4228 0.5461 0.5231 0.5376
Vacuole Periphery 0.1218 0.1429 0.1175 0.106 0.089 0.1388 0.1126 0.1525 0.1239 0.0947 0.0694 0.1701 0.1108 0.1222 0.1424 0.1171 0.1 0.115

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.1967 24.8233 20.1577 30.1724 22.417 30.895 26.1644 25.41 28.8634 30.7797
Translational Efficiency 1.2312 0.9043 1.3705 0.9627 1.021 1.0382 1.1344 1.1439 0.8937 0.9075

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
171 439 287 1226 1963 688 191 1526 2134 1127 478 2752

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 919.46 836.56 983.02 856.22 810.60 833.66 942.04 903.33 819.32 834.79 966.65 882.34
Standard Deviation 127.52 125.73 106.46 121.79 119.70 113.30 99.35 122.89 123.92 118.31 105.60 124.62
Intensity Change Log 2 -0.136318 0.096434 -0.102805 0.040469 0.216798 0.156263 -0.050784 0.154081 0.024384

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000708 0.000033 0.000834 0.001655 0.000006 0.000029 0.000308 0.001654 0.000062 0.000030 0.000624 0.001655
Bud Neck 0.012869 0.010625 0.014010 0.021973 0.004401 0.013136 0.022414 0.019453 0.005079 0.012158 0.017368 0.020575
Bud Site 0.022562 0.008734 0.026925 0.048007 0.003167 0.003650 0.013350 0.029794 0.004721 0.005630 0.021500 0.037908
Cell Periphery 0.000515 0.000410 0.000119 0.000108 0.000825 0.001038 0.000114 0.000074 0.000800 0.000794 0.000117 0.000089
Cytoplasm 0.112825* 0.021110 0.169182* 0.191366* 0.007092 0.009532 0.238219* 0.159443* 0.015564 0.014042 0.196768* 0.173665*
Cytoplasmic Foci 0.066472 0.016857 0.061448 0.073866 0.005478 0.009295 0.059373 0.063221 0.010365 0.012240 0.060619 0.067963
Eisosomes 0.000071 0.000017 0.000065 0.000034 0.000006 0.000022 0.000070 0.000023 0.000011 0.000020 0.000067 0.000028
Endoplasmic Reticulum 0.003742 0.001763 0.005504 0.001560 0.001939 0.002716 0.004585 0.001059 0.002084 0.002344 0.005136 0.001282
Endosome 0.072613 0.070309 0.086713 0.118912 0.041314 0.058457 0.076016 0.170790 0.043822 0.063074 0.082439 0.147679
Golgi 0.004405 0.002587 0.001225 0.004707 0.000636 0.000980 0.000267 0.007104 0.000938 0.001606 0.000842 0.006036
Lipid Particles 0.002322 0.001878 0.007365 0.002741 0.000726 0.003372 0.005233 0.002226 0.000854 0.002790 0.006513 0.002455
Mitochondria 0.005774 0.001097 0.000647 0.004832 0.001315 0.001846 0.000276 0.003584 0.001672 0.001555 0.000498 0.004140
Mitotic Spindle 0.001897 0.001771 0.015750 0.023371 0.000116 0.000781 0.004465 0.031665 0.000259 0.001166 0.011241 0.027970
None 0.000103 0.000221 0.000630 0.000744 0.000057 0.000087 0.001337 0.000792 0.000060 0.000139 0.000913 0.000771
Nuclear Periphery 0.001637 0.000114 0.003271 0.001164 0.000106 0.000376 0.002451 0.001276 0.000228 0.000274 0.002943 0.001226
Nuclear Periphery Foci 0.004453 0.000359 0.013148 0.001897 0.000073 0.000220 0.009156 0.001168 0.000424 0.000274 0.011553 0.001493
Nucleolus 0.001252 0.000376 0.003363 0.001666 0.000437 0.001362 0.003598 0.001667 0.000503 0.000978 0.003457 0.001667
Nucleus 0.029631 0.004061 0.078828 0.023481 0.001995 0.007816 0.106458 0.024721 0.004210 0.006353 0.089869 0.024169
Peroxisomes 0.005903 0.002444 0.006244 0.013528 0.000325 0.001048 0.004504 0.010215 0.000772 0.001591 0.005549 0.011691
Vacuole 0.642858* 0.820501* 0.499744* 0.458783* 0.886841* 0.862527* 0.446610* 0.460797* 0.867290* 0.846156* 0.478513* 0.459900*
Vacuole Periphery 0.007387 0.034733 0.004985 0.005606 0.043147 0.021710 0.001198 0.009272 0.040281 0.026783 0.003471 0.007639

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.97 -1.03 -1.10 -2.19 -0.53 -3.18 -6.66 -1.86 -1.82 -1.25 0.76 -4.87 -2.67 -2.71 -1.15
Bud Neck -0.36 -1.82 -4.70 -4.82 -3.38 -4.67 -3.68 -11.67 -2.91 -0.31 -5.36 -6.10 -14.85 -5.13 -2.23
Bud Site 1.60 -1.02 -3.29 -8.64 -2.01 -0.79 -4.33 -12.06 -11.70 -5.46 -1.69 -4.67 -15.12 -14.21 -3.15
Cell Periphery -0.27 0.99 1.13 6.58 2.14 -0.62 7.59 8.10 2.95 3.89 -0.03 7.57 8.05 3.54 4.42
Cytoplasm 8.07 -2.86 -5.77 -25.03 -2.40 -1.33 -13.90 -28.84 -27.97 2.49 1.35 -18.44 -37.27 -37.82 0.10
Cytoplasmic Foci 5.95 -0.20 -1.15 -12.58 -1.16 -2.12 -8.41 -20.74 -16.48 -1.50 -1.77 -12.79 -25.15 -20.88 -1.63
Eisosomes 5.68 -0.38 3.51 -3.26 5.43 -5.93 -14.07 -14.76 -0.59 10.39 -4.60 -16.59 -8.16 -2.83 10.78
Endoplasmic Reticulum 1.90 -2.91 2.35 1.11 8.26 -1.98 -6.03 6.43 4.44 7.53 -1.12 -9.11 5.46 4.37 11.47
Endosome -1.89 -2.23 -3.37 -1.40 -0.82 -3.49 -0.77 -15.25 -10.18 -9.15 -4.95 -3.90 -15.75 -8.68 -7.12
Golgi 0.14 1.29 -0.03 -0.26 -3.10 -1.03 5.02 -4.30 -3.76 -4.73 -1.91 0.55 -5.18 -3.30 -5.34
Lipid Particles 0.22 -4.41 -2.30 -1.16 3.64 -2.13 -8.68 -5.56 0.94 5.69 -2.29 -7.62 -7.22 0.28 5.23
Mitochondria 1.25 1.50 0.24 -1.99 -2.55 -0.75 2.02 -1.73 -1.02 -3.74 -0.09 2.06 -1.89 -2.04 -4.20
Mitotic Spindle 0.25 -2.84 -7.84 -7.44 -1.62 -1.30 -2.51 -9.37 -9.04 -6.41 -1.54 -3.79 -12.63 -11.80 -4.28
None 0.07 -3.36 -4.02 -3.56 -1.18 -0.71 -4.87 -3.38 -3.25 1.11 -1.18 -6.70 -5.28 -4.71 0.09
Nuclear Periphery 2.76 -3.48 -0.36 -8.72 4.68 -2.86 -9.34 -3.76 -2.98 3.56 -0.75 -12.07 -6.05 -5.75 5.57
Nuclear Periphery Foci 2.06 -3.30 0.84 -3.96 5.07 -2.48 -8.36 -8.54 -7.16 7.16 0.82 -8.54 -5.06 -6.15 7.35
Nucleolus 2.36 -3.06 -2.74 -4.20 1.38 -1.78 -3.05 -4.80 -1.56 1.26 -1.51 -4.71 -5.99 -3.42 1.95
Nucleus 5.39 -5.37 -0.66 -14.88 5.87 -4.46 -9.63 -14.96 -11.03 7.25 -1.83 -13.57 -19.42 -17.25 9.21
Peroxisomes 0.79 -0.67 -2.59 -4.69 -2.71 -3.02 -3.83 -8.51 -7.51 -4.24 -2.29 -5.72 -10.78 -8.59 -4.32
Vacuole -6.58 6.70 8.45 23.94 0.80 2.80 21.60 45.72* 34.59* -1.20 3.03 29.87 53.37* 41.95* -0.09
Vacuole Periphery -8.57 0.39 0.69 10.14 0.79 8.12 23.95 19.25 7.26 -8.18 5.66 22.22 20.50 12.31 -4.66