Standard name
Description ER protein with chaperone and co-chaperone activity; involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.12 0.13 0.3 0.29 0.37 0.49 0.52 0.54 0.57 0.64 0.62 0.59 0.62 0.82 0.35 0.29 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07 0 0 0 0 0 0
Endosome 0 0 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0.05 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.23 0.22 0.06 0.06 0.12 0.08 0.09 0.14 0.16 0.15 0.13 0 0 0 0.08 0.09 0.06 0 0 0 0 0 0
Nucleus 0.21 0.11 0.07 0.07 0 0.06 0.06 0.06 0 0.05 0 0.05 0 0 0 0.42 0.36 0.31 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.78 0.8 0.76 0.81 0.8 0.73 0.71 0.62 0.57 0.54 0.45 0.47 0.45 0.39 0.19 0.44 0.47 0.64 0.87 0.9 0.89 0.87 0.84 0.8
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 1 0 1
Bud 0 0 1 0 0 0 0 0 0 1 1 1 2 2 1 0 0 1 0 1 0 1 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 2 2 1 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 30 36 29 54 79 108 211 266 201 183 233 264 113 113 80 74 51 25 0 2 0 1 7 6
Endoplasmic Reticulum 0 0 0 0 1 0 4 1 0 1 0 2 3 0 0 10 11 9 0 0 0 0 3 2
Endosome 5 0 7 10 16 7 7 2 1 0 0 1 7 6 1 4 6 7 16 12 7 12 19 27
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 1 1 1 2 0 3
Mitochondria 27 67 47 10 16 35 33 44 52 52 55 55 0 0 0 17 15 8 12 6 8 5 12 15
Nucleus 70 32 15 13 9 17 26 32 16 16 12 22 5 5 3 87 62 43 7 2 0 1 4 2
Nuclear Periphery 2 2 0 0 1 0 4 5 8 3 2 6 0 0 0 2 0 0 6 0 0 1 4 2
Nucleolus 0 0 0 0 0 1 0 0 0 1 1 1 0 0 0 0 0 1 0 0 0 1 1 0
Peroxisomes 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 2 3 0 0 0 0 0 1 1 0 0 0 0 0 1 0 1 2 2
Vac/Vac Membrane 260 231 164 146 221 213 309 317 212 172 164 203 85 72 19 93 81 89 320 275 206 208 328 293
Unique Cell Count 333 290 217 180 275 292 434 514 371 321 362 428 191 183 98 209 173 138 369 305 231 239 391 367
Labelled Cell Count 395 368 263 233 345 383 597 667 490 429 468 556 218 202 106 288 226 186 369 305 231 239 391 367


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 5.6 5.7 6.4 6.8 5.7 5.6 5.6 5.1 4.8 4.9 5.1 6.2 6.2 5.9 5.1 5.8 6.8 7.0 6.7 7.1
Std Deviation (1e-4) 1.3 1.2 1.6 1.8 1.7 1.6 1.5 1.6 1.3 1.1 1.4 1.2 1.4 1.4 1.7 1.1 1.4 1.6 1.7 1.5 1.9
Intensity Change (Log2) – – – 0.18 0.26 0.01 -0.01 -0.02 -0.15 -0.24 -0.2 -0.16 0.13 0.13 0.06 -0.15 0.03 0.27 0.3 0.25 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4.1 4.1 5.9 8.8 9.7 9.8 10.2 11.9 11.6 9.7 10.1 11.8 5.3 3.9 1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 1.1 1.4 -0.6 0 0 0 0 0 0 0.2 0 0 -0.9 0.1 0.9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -4.6 -5.2 -2.9 -5.1 -4.9 -2.4 0 -2.0 -2.9 -6.8 -6.7 -5.0 -3.9 -3.5 -4.0
Nucleus 0.1 -1.9 -0.5 -0.5 -0.3 -1.4 -0.9 -2.0 -0.9 -2.0 -1.9 -1.4 8.4 7.1 6.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.3 1.3 -0.7 -1.2 -3.6 -4.5 -5.2 -7.1 -6.8 -6.4 -7.3 -9.4 -6.6 -5.8 -2.2