Standard name
Human Ortholog
Description Subunit of a complex that associates with actin filaments; forms a complex with Tda2p that inhibits barbed end F-actin assembly; elevates actin monomer pools to increase endocytotic efficiency and to regulate the distribution of actin between cables and patches; Aim21p/Tda2p forms a larger complex with actin capping proteins Cap1p and Cap2p; involved in mitochondrial migration along actin filaments; recruited to cortical actin patches by SH3 domain-containing proteins Bbc1p and Abp1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.65 0.65 0.62 0.62 0.54 0.56 0.48 0.43 0.26 0.37 0.21 0.46 0.63 0.61 0.51 – – – 0.73 0.68 0.57 0.6 0.63 0.66
Bud 0.16 0.15 0.1 0.19 0.16 0.13 0.16 0.14 0.09 0.17 0.12 0.11 0.14 0.12 0.1 – – – 0.06 0 0 0.11 0.12 0.09
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0.05 0.1 0.07 0.06
Bud Site 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0.07 0.09 0.08 0.07 0.09 0.11 0.06 0.06 0 0.09 0 0 0.1 0.17 0.14 – – – 0 0 0 0 0 0
Cytoplasm 0.17 0.16 0.2 0.12 0.31 0.11 0.17 0.2 0.1 0.13 0.12 0.09 0.23 0.19 0.3 – – – 0 0.07 0 0.06 0 0.07
Endoplasmic Reticulum 0.1 0.12 0.1 0.09 0.08 0.16 0.24 0.15 0.15 0.12 0.12 0.17 0.11 0.12 0.15 – – – 0 0 0 0 0 0
Endosome 0 0 0.05 0.05 0 0 0 0 0 0 0.07 0 0 0 0 – – – 0 0.05 0.09 0 0.05 0
Golgi 0 0.16 0.12 0.08 0.07 0.08 0.14 0.06 0.07 0.06 0 0.11 0.11 0.16 0.11 – – – 0 0 0 0 0 0
Mitochondria 0.22 0.05 0 0.08 0 0.19 0.18 0.16 0.59 0.4 0.66 0.46 0 0 0 – – – 0 0 0.08 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0.21 0.08 0.09 0.25 0.08 0.11 0.05 0 0 0.07 0.05 0 0.14 0.13 0.09 – – – 0.05 0 0.05 0 0 0
SpindlePole 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 191 138 67 80 74 120 107 119 35 77 29 76 143 172 158 – – – 240 179 87 137 240 296
Bud 48 33 11 24 22 28 36 37 12 35 16 19 31 34 32 – – – 18 10 5 25 45 39
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – – – 7 10 7 21 26 26
Bud Site 16 2 1 4 4 6 5 5 0 3 0 4 5 4 0 – – – – – – – – –
Cell Periphery 21 20 9 9 13 23 14 16 1 18 4 5 22 47 42 – – – 0 1 1 0 1 2
Cytoplasm 50 35 22 15 43 23 38 54 13 28 17 15 53 53 93 – – – 12 17 5 12 13 31
Endoplasmic Reticulum 30 26 11 12 11 35 54 42 20 25 17 29 24 33 47 – – – 2 2 3 1 4 7
Endosome 1 9 5 6 2 2 2 9 5 8 10 6 6 1 5 – – – 7 13 13 10 20 12
Golgi 13 34 13 10 10 17 30 17 9 13 5 19 24 44 35 – – – 5 10 3 2 6 3
Mitochondria 66 10 4 10 4 42 39 45 79 84 92 76 2 3 1 – – – 9 6 11 5 10 8
Nucleus 1 0 2 1 0 1 1 0 0 0 0 1 0 1 2 – – – 0 0 0 1 1 0
Nuclear Periphery 3 0 0 0 0 0 0 0 2 0 1 0 0 0 0 – – – 0 0 0 0 0 1
Nucleolus 0 0 0 1 0 0 2 7 0 2 1 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 62 18 10 32 11 23 11 6 4 14 7 5 32 36 27 – – – 17 4 7 4 2 10
SpindlePole 4 5 2 9 5 2 4 7 0 3 1 1 9 10 9 – – – 1 0 0 2 2 1
Vac/Vac Membrane 1 1 1 3 1 2 4 15 7 7 2 3 1 2 1 – – – 2 2 1 2 2 5
Unique Cell Count 295 213 108 129 138 216 221 274 133 209 139 166 227 282 310 329 263 153 230 382 450
Labelled Cell Count 507 331 159 216 200 324 347 379 187 317 202 259 352 440 452 329 263 153 230 382 450


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 9.9 8.9 9.1 9.6 8.1 8.4 8.6 7.1 7.5 7.0 7.3 10.1 10.3 9.6 – – – 9.3 9.2 9.5
Std Deviation (1e-4) 1.1 1.8 1.5 2.0 1.9 1.6 1.6 1.6 1.6 1.5 2.1 1.4 1.6 1.7 1.6 – – – 1.5 1.4 1.5
Intensity Change (Log2) – – – 0.04 0.11 -0.12 -0.08 -0.05 -0.31 -0.23 -0.35 -0.29 0.19 0.22 0.12 – – – 0.07 0.06 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -1.3 -1.1 -2.3 -3.3 -5.6 -4.3 -6.6 -2.6 0.2 -0.2 -2.0 – – –
Bud 1.8 1.3 0.7 1.5 0.9 -0.3 1.6 0.3 0.3 0.9 0.5 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery -0.4 0.3 0.7 -0.7 -0.9 0 0.1 -1.9 -2.0 0.4 2.1 1.4 – – –
Cytoplasm -1.8 1.9 -2.4 -0.7 -0.1 -2.3 -1.6 -1.7 -2.7 0.6 -0.4 1.9 – – –
Endoplasmic Reticulum -0.2 -0.6 1.5 3.0 1.3 1.1 0.5 0.5 1.7 0.1 0.4 1.3 – – –
Endosome 0 0 0 0 0 0 0 0.8 0 0 0 0 – – –
Golgi -1.1 -1.3 -1.2 0.4 -1.9 -1.4 -1.8 -2.5 -0.1 -0.4 0.9 -0.2 – – –
Mitochondria 1.3 0 3.8 3.5 3.3 9.0 6.9 10.0 7.5 0 0 0 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 3.1 -0.4 0.4 -1.5 0 -2.1 -0.8 -1.3 -2.2 1.2 1.0 -0.2 – – –
SpindlePole 1.9 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5051 3.016 2.9681 3.122 2.2928 2.7506 2.9717 3.6646 3.5711 3.0899 2.7715 3.4586 3.8796 4.2056 4.4034 4.3832 4.1683 4.2539
Actin 0.7981 0.6189 0.7906 0.7433 0.6287 0.7376 0.7138 0.6417 0.7546 0.702 0.7535 0.8601 0.8154 0.6348 0.7946 0.811 0.735 0.858
Bud 0.0082 0.0132 0.0322 0.024 0.0145 0.0015 0.0065 0.0073 0.0366 0.0209 0.0094 0.0034 0.0018 0.0161 0.0227 0.0144 0.0114 0.0009
Bud Neck 0.0065 0.0044 0.0138 0.0035 0.0015 0.1035 0.0189 0.0083 0.0128 0.018 0.0042 0.0612 0.0048 0.004 0.0165 0.0123 0.0158 0.0637
Bud Periphery 0.0078 0.012 0.056 0.0564 0.0593 0.0028 0.0106 0.0066 0.0647 0.0663 0.0085 0.0081 0.0025 0.0156 0.0398 0.0157 0.0101 0.001
Bud Site 0.0139 0.0801 0.031 0.0104 0.0026 0.0018 0.0614 0.2161 0.0527 0.0259 0.0059 0.0065 0.0175 0.1268 0.0386 0.0181 0.0057 0.0012
Cell Periphery 0.001 0.0012 0.0006 0.001 0.0007 0.0006 0.001 0.0014 0.0011 0.0013 0.0002 0.0007 0.0003 0.001 0.0004 0.0003 0.0009 0.0002
Cytoplasm 0.0345 0.0748 0.0158 0.0366 0.0456 0.0183 0.0086 0.0375 0.0235 0.0095 0.0856 0.0088 0.0045 0.0907 0.023 0.0117 0.0574 0.0095
Cytoplasmic Foci 0.0232 0.0873 0.0098 0.0124 0.0651 0.0215 0.0414 0.0313 0.0095 0.0362 0.0271 0.0129 0.0317 0.0479 0.0214 0.0346 0.0754 0.0208
Eisosomes 0.0008 0.0014 0.0004 0.0005 0.0003 0.0007 0.0013 0.0011 0.0003 0.0005 0.0003 0.0006 0.0005 0.0002 0.0003 0.0003 0.0002 0.0005
Endoplasmic Reticulum 0.0077 0.0014 0.0005 0.0012 0.0005 0.0018 0.0011 0.0003 0.0004 0.0007 0.0045 0.0009 0.0037 0.0008 0.0002 0.0016 0.0009 0.0004
Endosome 0.0211 0.0204 0.015 0.0328 0.022 0.0264 0.0123 0.0032 0.0083 0.0249 0.0244 0.0054 0.0227 0.011 0.0076 0.0265 0.0343 0.0072
Golgi 0.0328 0.0295 0.0155 0.0233 0.0404 0.026 0.0115 0.0055 0.0092 0.0127 0.0249 0.0078 0.0202 0.018 0.0115 0.0203 0.0268 0.0124
Lipid Particles 0.0092 0.0032 0.0025 0.0116 0.0085 0.006 0.006 0.0008 0.0007 0.0124 0.0017 0.0005 0.0082 0.0005 0.0026 0.0014 0.0027 0.0014
Mitochondria 0.0081 0.0132 0.0056 0.0081 0.0722 0.0097 0.0442 0.0006 0.0015 0.005 0.0038 0.0018 0.0031 0.0016 0.0024 0.0032 0.0033 0.0012
None 0.0012 0.0072 0.0005 0.0014 0.0005 0.0013 0.0052 0.0092 0.0055 0.0036 0.0169 0.0005 0.0071 0.0007 0.0002 0.0008 0.0017 0.0008
Nuclear Periphery 0.0023 0.0004 0.0005 0.0006 0.0003 0.0018 0.0028 0.0001 0.0059 0.0068 0.0017 0.0001 0.0132 0.0001 0.0001 0.0034 0.0002 0.0008
Nucleolus 0.0001 0.0002 0.0003 0.0014 0.0001 0.0024 0.0037 0.0001 0.0001 0.0151 0 0 0.0005 0.0002 0.0001 0.0001 0.0002 0.0001
Nucleus 0.0003 0.0003 0.0004 0.0008 0.0003 0.0054 0.0053 0.0002 0.0005 0.0102 0.0007 0.0001 0.0015 0.0004 0.0001 0.0014 0.0003 0.0003
Peroxisomes 0.0195 0.0287 0.0076 0.0128 0.0344 0.0241 0.0327 0.0279 0.0107 0.0118 0.0247 0.02 0.0334 0.028 0.0168 0.0191 0.0135 0.0178
Punctate Nuclear 0.001 0.0012 0.0006 0.0004 0.0011 0.0004 0.0059 0.0003 0.0007 0.0093 0.0006 0.0002 0.005 0.0004 0.0005 0.0019 0.0015 0.0015
Vacuole 0.0019 0.0007 0.0007 0.0147 0.0009 0.0045 0.005 0.0005 0.0005 0.0058 0.0008 0.0002 0.0014 0.0008 0.0005 0.0013 0.0016 0.0002
Vacuole Periphery 0.0009 0.0002 0.0002 0.0026 0.0006 0.0019 0.001 0 0.0002 0.0012 0.0005 0.0001 0.0009 0.0002 0.0001 0.0007 0.0011 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.258 19.8242 17.222 22.2559 24.1942 24.3263 31.8997 30.1917 31.6058 24.2989
Translational Efficiency 1.8936 1.764 1.7028 1.8439 1.4153 1.9851 0.9703 1.22 1.2178 1.371

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of a complex that associates with actin filaments; forms a complex with Tda2p that inhibits barbed end F-actin assembly; elevates actin monomer pools to increase endocytotic efficiency and to regulate the distribution of actin between cables and patches; Aim21p/Tda2p forms a larger complex with actin capping proteins Cap1p and Cap2p; involved in mitochondrial migration along actin filaments; recruited to cortical actin patches by SH3 domain-containing proteins Bbc1p and Abp1p
Localization
Cell Percentages actin (89%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Aim21

Aim21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aim21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available