Standard name
Description Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p; associates with actin patches on cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates Arp2/3 complex in vitro; phosphorylation of Thr-1225 is regulated by MAPK Hog1p in response to osmotic stress; previously thought to be a subunit of poly(A) ribonuclease

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.5 0.55 0.72 0.74 0.62 0.57 0.46 0.44 0.43 0.46 0.52 0.46 0.69 0.65 0.58 0.49 0.59 0.35 0.84 0.83 0.91 0.78 0.76 0.82
Bud 0.14 0.16 0.14 0.21 0.23 0.19 0.16 0.18 0.19 0.17 0.17 0.17 0.18 0.13 0.08 0.15 0.18 0 0 0.06 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0
Cell Periphery 0.14 0.14 0.13 0.09 0.07 0.07 0.05 0.06 0.07 0.06 0.11 0.06 0.22 0.21 0.36 0.07 0.08 0.06 0 0 0 0 0 0
Cytoplasm 0.09 0.13 0.06 0.05 0.07 0 0.05 0 0 0 0 0 0 0.08 0.09 0.22 0.13 0.24 0 0 0 0 0 0
Endoplasmic Reticulum 0.15 0.16 0.07 0.07 0.09 0.15 0.11 0.06 0 0.07 0.07 0.05 0.17 0.24 0.24 0 0.05 0.12 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0 0 0 0 0 0
Golgi 0.34 0.1 0.28 0.27 0.24 0.11 0.18 0.2 0.13 0.09 0.16 0.06 0.38 0.34 0.44 0.33 0.41 0.41 0 0 0 0.05 0.05 0.07
Mitochondria 0.06 0.17 0.1 0.31 0.19 0.54 0.51 0.57 0.73 0.72 0.77 0.76 0 0 0 0.07 0.11 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0.14 0.09 0.11 0.07 0.09 0.08 0.13 0.09 0.08 0.15 0 0 0 0.13 0.1 0.24 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 133 100 188 131 140 165 143 185 117 159 202 178 88 74 71 33 36 6 307 192 293 157 132 145
Bud 36 29 36 37 53 55 51 74 52 57 68 66 23 15 10 10 11 0 15 14 7 4 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 3 1 1 0 0
Bud Site 2 2 8 2 7 7 5 14 5 12 8 20 1 0 0 2 3 0
Cell Periphery 38 25 34 16 16 19 17 25 20 20 43 22 28 24 44 5 5 1 4 1 1 0 0 1
Cytoplasm 25 24 16 9 16 10 16 9 6 5 6 2 4 9 11 15 8 4 6 3 1 3 2 1
Endoplasmic Reticulum 40 30 19 13 21 45 34 25 9 25 26 18 21 27 30 1 3 2 6 4 1 7 3 2
Endosome 2 1 7 1 2 1 4 4 0 0 1 4 1 0 0 0 1 2 3 1 2 3 5 5
Golgi 90 19 73 47 54 32 57 83 34 32 61 23 48 38 54 22 25 7 6 3 5 10 9 11
Mitochondria 16 31 27 54 42 157 157 237 196 247 302 299 5 2 0 5 7 1 3 3 0 3 2 2
Nucleus 0 0 0 0 0 1 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 3 0 3 0 2 0 0 0 1 0 0 0 1 0 0 1 1
Nucleolus 3 1 5 0 4 6 5 12 8 10 9 3 0 0 0 2 0 1 0 0 0 0 0 0
Peroxisomes 0 0 37 15 25 21 29 34 35 30 31 57 3 5 5 9 6 4 0 0 4 2 7 4
SpindlePole 0 2 4 1 7 1 4 3 3 5 2 4 1 0 0 2 3 0 0 0 0 0 0 0
Vac/Vac Membrane 7 2 5 3 6 1 6 9 2 5 3 1 0 0 0 0 2 0 1 0 0 1 2 0
Unique Cell Count 266 182 260 176 226 291 310 418 269 345 391 391 127 113 123 67 61 17 368 232 322 201 174 177
Labelled Cell Count 392 266 459 329 393 521 528 717 487 610 764 700 223 194 225 107 110 28 368 232 322 201 174 177


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.6 24.0 18.2 17.5 16.8 15.5 15.1 14.7 13.3 13.0 13.1 12.4 21.3 21.7 22.0 13.5 15.9 20.6 21.3 22.7 25.0
Std Deviation (1e-4) 6.4 7.5 4.3 3.8 4.2 4.1 3.8 3.7 3.4 3.5 3.2 3.0 5.0 5.6 6.1 3.7 6.3 7.7 4.9 6.0 5.7
Intensity Change (Log2) -0.06 -0.11 -0.23 -0.27 -0.3 -0.45 -0.49 -0.47 -0.55 0.23 0.26 0.28 -0.42 -0.19 0.18 0.23 0.32 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.5 -2.4 -3.8 -6.3 -7.1 -6.7 -6.5 -5.3 -6.7 -0.6 -1.3 -2.8 -3.6 -2.0 -3.2
Bud 2.0 2.7 1.6 0.9 1.3 1.7 0.9 1.2 1.0 1.1 -0.1 -1.6 0.2 0.8 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 -0.5 -1.2 0.2 -0.9 0.3 -0.8 1.3 0 0 0 0 0 0
Cell Periphery -1.3 -2.2 -2.6 -3.2 -3.2 -2.1 -3.1 -0.8 -3.3 2.3 2.0 5.1 -1.3 -1.0 0
Cytoplasm -0.5 0.4 -1.5 -0.5 -2.7 -2.3 -3.1 -3.2 -4.3 -1.3 0.6 1.0 4.0 0 0
Endoplasmic Reticulum 0 0.8 3.0 1.5 -0.7 -2.0 0 -0.3 -1.5 2.8 4.5 4.7 0 0 0
Endosome 0 0 0 -1.2 0 0 0 0 0 0 0 0 0 0 0
Golgi -0.3 -1.0 -5.1 -2.7 -2.5 -4.4 -6.0 -3.9 -7.8 1.9 1.1 3.1 0.8 2.0 0
Mitochondria 5.3 2.6 10.8 10.2 12.0 14.5 15.0 16.7 16.5 -2.2 -2.9 -3.7 -0.7 0.2 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0.1 0 0.8 0.8 0.8 0.3 0 0 0 0 0 0 0
Peroxisomes -1.8 -1.0 -2.7 -1.8 -2.5 -0.4 -2.1 -2.6 0.1 -3.6 -2.8 -3.0 -0.2 -0.9 0
SpindlePole 0 1.2 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.5 0 0 0.2 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pan1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available