Standard name
Human Ortholog
Description Specific chaperone for ribosomal protein Rpl10p; binds nascent Rpl10p during translation; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.12 0.1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.48 0.89 0.48 0.49 0.32 0.47 0.42 0.39 0.51 0.33 0.35 0.47 0.67 0.55 0.6 0.29 0.32 0.25 0.49 0.35 0.4 0.38 0.27 0.28
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08
Golgi 0 0 0 0.08 0.1 0 0 0.05 0.06 0 0 0 0 0.06 0.11 0.19 0.17 0.24 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.1 0.09 0.08 0.15 0.24 0.2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0.24 0.09 0.12 0.14 0.13 0.14 0.1 0.15 0.09 0 0.06 0.06 0.07 0 0 0.39 0.47 0.35 0.42 0.31 0.26
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.13
Vac/Vac Membrane 0.63 0.16 0.63 0.41 0.61 0.44 0.38 0.4 0.32 0.43 0.34 0.29 0.55 0.57 0.49 0.67 0.66 0.62 0.06 0.11 0.14 0.08 0.1 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 5 0 1 2 2 1 0 0 0 0 1 0 0 0 2 0 1 1
Bud 0 0 0 0 0 1 3 5 1 5 2 3 0 0 0 0 1 0 0 4 7 24 92 69
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3
Bud Site 0 0 0 0 0 0 0 1 0 1 2 1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1 0 1 3 1 3 0 1 2 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 35 192 153 64 77 156 161 186 152 125 109 120 288 334 362 78 87 53 41 79 135 150 201 187
Endoplasmic Reticulum 0 0 3 0 0 1 3 12 4 5 3 6 2 2 1 13 13 9 0 2 5 2 1 6
Endosome 0 0 0 0 1 0 3 0 0 1 1 0 1 1 1 0 0 0 0 0 3 4 43 53
Golgi 2 0 10 10 25 12 4 23 17 2 4 5 8 37 66 51 45 52 0 0 0 1 1 2
Mitochondria 0 0 1 0 2 19 39 44 23 58 77 51 0 0 1 3 0 2 0 0 2 0 0 2
Nucleus 0 6 11 31 23 41 54 63 42 37 46 24 11 36 36 18 4 9 33 105 118 163 235 170
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 2 5 2 6 6
Nucleolus 1 1 0 0 1 1 3 4 0 7 4 2 1 1 0 8 14 17 0 0 1 2 12 8
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 3
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 5 69 85
Vac/Vac Membrane 46 35 201 53 148 144 143 194 97 165 108 74 239 349 298 182 180 133 5 25 47 29 75 60
Unique Cell Count 73 216 317 130 243 330 379 480 299 380 315 258 433 607 604 271 272 216 86 227 340 392 759 669
Labelled Cell Count 84 234 379 158 278 376 419 535 338 411 358 289 553 761 766 353 345 275 86 227 340 392 759 669


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.7 30.7 23.9 20.3 18.7 16.1 14.7 14.8 14.2 13.6 13.3 13.0 23.8 23.0 20.3 33.7 32.1 34.9 23.2 23.7 23.1
Std Deviation (1e-4) 4.8 5.0 5.2 5.4 4.7 3.9 3.4 3.7 3.5 3.5 3.7 3.2 8.6 8.1 7.6 11.2 10.1 9.8 8.4 8.9 9.2
Intensity Change (Log2) -0.24 -0.35 -0.57 -0.7 -0.69 -0.75 -0.81 -0.84 -0.88 -0.0 -0.05 -0.23 0.5 0.43 0.55 -0.04 -0.01 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 -4.0 -0.3 -1.5 -2.7 0.6 -4.1 -3.5 -0.4 5.0 2.0 3.4 -4.8 -4.0 -5.5
Endoplasmic Reticulum 0 0 0 0 1.6 0 0 0 0 0 0 0 2.9 2.9 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 2.1 3.5 0.3 -2.0 1.1 1.5 -2.7 -1.6 -0.9 -1.2 1.9 4.1 6.2 5.6 7.4
Mitochondria 0 0 4.0 5.6 5.3 4.7 7.1 9.2 8.1 0 0 0 0 0 0
Nucleus 6.7 2.9 4.2 4.9 4.6 4.7 3.3 4.9 2.9 -0.7 1.6 1.6 1.8 -1.5 0.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 4.1 5.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.4 -0.6 -5.0 -6.7 -6.4 -7.7 -5.3 -7.3 -8.3 -2.3 -1.7 -4.1 1.0 0.7 -0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 21.6944 28.0211 24.9157 21.6141 23.0447 24.5591 18.6267 27.2651 23.8677 19.585 25.2234 23.8229 25.164 29.2112 27.9901 26.0021 26.343 26.8448
Actin 0.0112 0.0001 0.0007 0.0008 0.0086 0.0001 0.0274 0.0002 0.0168 0.0298 0.0044 0.0022 0.007 0.0002 0.001 0.0001 0.0047 0.0009
Bud 0.0009 0 0.0003 0.0002 0.0032 0.0001 0.0013 0.0001 0.0005 0.0045 0.0001 0.0003 0.0003 0.0001 0.0003 0.0002 0.0002 0.0001
Bud Neck 0.0007 0.0002 0.0003 0.0002 0.0004 0.0008 0.0161 0.0002 0.0005 0.0027 0.0006 0.0012 0.001 0.0002 0.0002 0.0004 0.0005 0.0007
Bud Periphery 0.0005 0 0.0001 0.0001 0.0048 0.0001 0.0015 0 0.0006 0.0109 0.0001 0.0002 0.0002 0 0.0003 0.0001 0.0001 0.0002
Bud Site 0.0016 0.0001 0.0002 0.0002 0.0139 0 0.01 0.0001 0.0005 0.0116 0.0003 0.0004 0.0008 0.0001 0.0031 0.0001 0.0003 0.0001
Cell Periphery 0.0001 0 0 0.0001 0.0002 0 0.0005 0 0.0001 0.0011 0 0 0.0001 0 0 0.0001 0.0001 0.0001
Cytoplasm 0.3836 0.5813 0.5041 0.4783 0.4656 0.4044 0.2438 0.4578 0.3166 0.2568 0.4832 0.3032 0.2696 0.4213 0.322 0.2986 0.3374 0.2253
Cytoplasmic Foci 0.0055 0.0001 0.0006 0.0049 0.0034 0.0001 0.0305 0.0004 0.0063 0.0218 0.0105 0.0015 0.0125 0.0002 0.001 0.0006 0.0022 0.0002
Eisosomes 0.0001 0 0 0 0.0001 0 0.0003 0 0.0001 0.0003 0 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0044 0.0002 0.0011 0.006 0.0014 0.0016 0.008 0.0003 0.0013 0.0059 0.0005 0.0007 0.0039 0.0006 0.0006 0.0011 0.0024 0.0016
Endosome 0.0042 0.0001 0.0005 0.0103 0.0037 0.0006 0.0257 0.0001 0.0094 0.0157 0.0068 0.0013 0.0108 0.0001 0.0005 0.0007 0.0022 0.0021
Golgi 0.0011 0 0.0001 0.0007 0.0018 0 0.0069 0.0001 0.0015 0.011 0.0058 0.0011 0.0017 0 0.0001 0 0.0021 0.0001
Lipid Particles 0.0016 0 0.0003 0.0013 0.0039 0 0.016 0.0001 0.0042 0.0196 0.0181 0.0035 0.006 0 0.0002 0.0001 0.012 0.0001
Mitochondria 0.0025 0.0001 0.0005 0.0011 0.0015 0.0003 0.0059 0.0003 0.0025 0.0324 0.0011 0.0009 0.0036 0.0002 0.0003 0.0003 0.0053 0.0025
None 0.0029 0.0001 0.0004 0.0134 0.0008 0.0001 0.0106 0.0001 0.0086 0.0043 0.0003 0.0002 0.0036 0.0002 0.0005 0.0006 0.0011 0.0002
Nuclear Periphery 0.0458 0.0154 0.0246 0.0284 0.0183 0.0383 0.0429 0.018 0.0213 0.0229 0.0227 0.0295 0.0365 0.0207 0.0185 0.022 0.0337 0.0302
Nucleolus 0.0005 0.0001 0.0003 0.0002 0.0007 0.0002 0.0044 0.0007 0.0003 0.0015 0.0001 0.0007 0.0016 0.0003 0.0004 0.0012 0.0004 0.0003
Nucleus 0.524 0.4011 0.4628 0.4473 0.4609 0.5493 0.4931 0.5173 0.5968 0.5086 0.4338 0.6464 0.6207 0.554 0.6479 0.6674 0.5913 0.7326
Peroxisomes 0.0004 0 0 0.0001 0.002 0 0.0142 0.0001 0.0058 0.0154 0.0084 0.0011 0.0017 0 0.0002 0 0.0007 0
Punctate Nuclear 0.0055 0.0006 0.0016 0.0013 0.0029 0.0007 0.0323 0.0037 0.0027 0.0085 0.0025 0.0034 0.0101 0.0012 0.0022 0.0032 0.0022 0.001
Vacuole 0.002 0.0003 0.001 0.0037 0.0014 0.0022 0.0061 0.0003 0.0025 0.0096 0.0005 0.0016 0.0062 0.0005 0.0004 0.0026 0.0007 0.0011
Vacuole Periphery 0.0009 0.0001 0.0004 0.0013 0.0004 0.0008 0.0026 0.0001 0.0009 0.005 0.0002 0.0004 0.002 0.0001 0.0001 0.0007 0.0004 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 83.4364 85.2424 79.0669 75.1898 94.3807 58.7135 79.0702 82.5826 72.5236 79.1672
Translational Efficiency 1.9346 1.9883 1.716 1.7925 1.358 1.9107 1.6138 1.1302 1.2559 1.3558

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency