ORF
Human Ortholog
Description Putative phosphatidylinositol/phosphatidylcholine transfer protein; possibly involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study; YKL091C has a paralog, SEC14, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.34 0.36 0.5 0.49 0.47 0.33 0.26 0.21 0.22 0.16 0.16 0.83 0.6 0.61 0.28 0.28 0.21 0.38 0.2 0.3 0.69 0.68 0.69
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.35 0.09 0.09 0 0.28 0.4 0.41 0.59 0.49 0.67 0.44 0 0 0 0 0 0 0.05 0 0 0 0 0
Nucleus 0.2 0.29 0.33 0.35 0.26 0.33 0.41 0.37 0.47 0.44 0.54 0.08 0.07 0.12 0.36 0.25 0.35 0.31 0.44 0.33 0.11 0.08 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.31 0.52 0.18 0.19 0.15 0.17 0.07 0.09 0.07 0.05 0.1 0.12 0.45 0.39 0.48 0.58 0.47 0.16 0.28 0.19 0.07 0.13 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 1 0 1 0 2 1 5 8 10 12 7 0 0 0 0 0 0 2 2 0 0 2 6
Bud Neck 0 0 0 2 0 2 3 0 5 0 1 1 0 0 0 0 0 0 0 0 0 2 3
Bud Site 0 0 0 0 0 0 4 6 10 2 7 0 0 0 0 0 0
Cell Periphery 0 0 0 1 1 0 1 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0
Cytoplasm 37 21 115 151 135 157 116 75 98 59 54 49 79 71 54 50 31 47 18 13 27 96 120
Endoplasmic Reticulum 1 1 0 3 0 0 0 0 0 0 0 0 1 0 13 6 5 0 0 1 0 0 1
Endosome 1 0 0 2 3 1 1 1 0 0 0 0 3 3 4 12 3 1 0 0 0 0 2
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 1 2 0 0 0 0 0 1
Mitochondria 38 5 21 10 79 188 181 211 221 246 151 0 2 0 6 1 4 5 0 0 0 1 2
Nucleus 22 17 76 108 73 159 182 133 213 161 186 5 9 14 71 45 52 39 41 14 4 11 3
Nuclear Periphery 0 0 0 0 0 1 1 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 6 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 1 0 6 2 3 1 2 0 0 0 0 0 1 4 0 1 0 2 4
Vac/Vac Membrane 33 30 41 60 44 79 30 31 32 20 33 7 59 45 94 104 71 19 26 8 2 18 19
Unique Cell Count 108 58 229 309 286 475 440 355 451 365 343 59 131 116 196 180 150 125 95 44 39 141 175
Labelled Cell Count 139 74 255 338 338 589 530 469 592 504 442 62 153 134 244 220 170 125 95 44 39 141 175


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 5.2 5.1 5.3 4.5 4.3 4.5 4.1 4.3 3.9 4.4 5.7 7.2 8.1 7.4 8.5 8.4 5.9 6.2
Std Deviation (1e-4) 2.0 0.9 1.2 1.4 1.6 1.5 1.3 1.6 1.1 1.4 1.3 1.1 3.9 16.7 1.5 2.0 2.0 1.7 1.4
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1564 0.4762 0.7071 0.2924 0.6623 0.3466 0.261 0.9192 0.862 0.7811 0.5759 0.6557 -0.3331 0.3882 0.1212 0.2988 -0.2123 0.2357
Actin 0.0284 0.0005 0.0194 0.0275 0.014 0.0153 0.0192 0.0001 0.0115 0.0347 0.0053 0.0017 0.0077 0.0003 0.0003 0.0006 0.0001 0.0004
Bud 0.0037 0.004 0.0041 0.0038 0.0094 0.0007 0.0005 0.0003 0.0031 0.0005 0.0123 0.0002 0.0002 0.0109 0.0001 0.0001 0.0001 0.0023
Bud Neck 0.0114 0.0005 0.0013 0.0037 0.0018 0.0039 0.0225 0.0004 0.0107 0.0181 0.0082 0.0028 0.0007 0.0006 0.0004 0.0009 0.0013 0.0023
Bud Periphery 0.0045 0.0017 0.0077 0.0026 0.0171 0.002 0.0006 0.0003 0.0023 0.0009 0.0163 0.0002 0.0001 0.0016 0 0 0 0.001
Bud Site 0.0109 0.0066 0.0045 0.0046 0.0069 0.0006 0.004 0.0024 0.0097 0.0012 0.0053 0.0003 0.0018 0.0102 0.0005 0.0004 0.0001 0.0002
Cell Periphery 0.0005 0.0001 0.0003 0.0003 0.0005 0.0001 0.0002 0.0001 0.0003 0.0001 0.0008 0 0.0001 0.0002 0 0 0 0.0001
Cytoplasm 0.2025 0.3895 0.2381 0.2084 0.2287 0.2762 0.2836 0.3431 0.3465 0.3151 0.2975 0.3665 0.4018 0.4323 0.3976 0.2195 0.2495 0.37
Cytoplasmic Foci 0.04 0.0065 0.0179 0.0149 0.0292 0.0111 0.0407 0.0034 0.0132 0.0095 0.0377 0.0108 0.0065 0.0045 0.0043 0.0122 0.0006 0.0057
Eisosomes 0.0003 0 0.0003 0.0004 0.0003 0.0001 0.0002 0 0.0002 0.0001 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0126 0.0044 0.0061 0.004 0.0072 0.0038 0.0054 0.0031 0.0041 0.0036 0.0048 0.0026 0.0095 0.0045 0.008 0.0027 0.0012 0.0036
Endosome 0.0509 0.004 0.0231 0.0101 0.0612 0.0079 0.032 0.0017 0.0113 0.0069 0.0404 0.0075 0.006 0.0067 0.0064 0.0033 0.0012 0.0047
Golgi 0.0096 0.0002 0.0069 0.0036 0.0166 0.0022 0.0071 0.0001 0.0054 0.01 0.0108 0.0043 0.0004 0.0002 0.0001 0.0002 0 0.0001
Lipid Particles 0.0216 0.0002 0.0101 0.0083 0.0408 0.0048 0.0365 0.0001 0.0049 0.0187 0.0289 0.0109 0.0005 0.0001 0.0003 0.0001 0 0.0002
Mitochondria 0.0099 0.0008 0.0081 0.0035 0.028 0.0026 0.0134 0.0004 0.004 0.005 0.0037 0.0038 0.0005 0.0007 0.0004 0.0005 0.0003 0.0016
None 0.2417 0.3036 0.2803 0.2139 0.1705 0.2617 0.3509 0.4042 0.3119 0.1349 0.22 0.1844 0.1837 0.1236 0.1596 0.1507 0.0035 0.0421
Nuclear Periphery 0.0549 0.014 0.0214 0.0229 0.0347 0.0146 0.0153 0.0056 0.0101 0.0164 0.0152 0.0072 0.0269 0.0162 0.021 0.0145 0.0134 0.0139
Nucleolus 0.0077 0.0017 0.003 0.0033 0.0056 0.0024 0.0019 0.001 0.0018 0.0012 0.0051 0.0015 0.0045 0.001 0.0009 0.0017 0.0008 0.002
Nucleus 0.2131 0.2387 0.2874 0.3845 0.1899 0.3324 0.1324 0.2259 0.2207 0.3751 0.1959 0.3726 0.3341 0.3724 0.3881 0.5791 0.7127 0.5203
Peroxisomes 0.0221 0.002 0.0143 0.0043 0.0488 0.0074 0.0134 0.0001 0.0072 0.0384 0.022 0.0084 0.0001 0.0001 0.0001 0.0015 0 0.0001
Punctate Nuclear 0.0414 0.0175 0.0404 0.0729 0.0786 0.0474 0.0152 0.0059 0.0171 0.0069 0.0538 0.0117 0.0094 0.0106 0.0087 0.0101 0.0133 0.0264
Vacuole 0.0082 0.0031 0.0037 0.0018 0.0066 0.0021 0.0039 0.0017 0.0033 0.0021 0.0133 0.0023 0.0048 0.0029 0.0026 0.0014 0.0016 0.0023
Vacuole Periphery 0.0042 0.0005 0.0017 0.0006 0.0036 0.0007 0.0011 0.0002 0.0008 0.0007 0.0027 0.0004 0.0006 0.0004 0.0005 0.0003 0.0002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.2785 6.3433 10.9829 14.2375 9.9668 8.2739 8.8188 10.4612 22.7842 14.9014
Translational Efficiency 1.8484 1.8647 1.2309 1.7255 1.3403 3.4619 2.4533 2.3075 1.3268 1.4707

Hit Data

Dataset Hit
Protein Abundance
Protein Localization
Gene Expression
Translational Efficiency