Standard name
Human Ortholog
Description Dual specificity protein phosphatase; regulates growth, sporulation, and glycogen accumulation in a cAMP-dependent protein kinase cascade dependent manner; mutants are defective in 60S ribosome assembly; positively regulates pre-autophagosomal structure (PAS) formation upon nitrogen starvation or rapamycin treatment

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.06 0.12 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.97 0.97 0.96 0.94 0.88 0.9 0.8 0.84 0.7 0.8 0.98 1.0 0.99 0.97 0.92 0.96 0.95 0.97 0.94 0.97 0.97 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.15 0.09 0.29 0.23 0.43 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 0 1 1 1 0 0 0 0 0 0 0 1 0 0 1 0 1
Bud 0 2 4 0 2 0 7 7 6 9 20 12 0 2 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 4 6 13 14 7 7 8 8 7 7 12 13 13 19 38 12 41 0 0 0 0 0 0
Cytoplasm 239 232 185 308 571 448 486 503 395 394 411 430 506 666 764 310 201 341 228 229 180 405 546 688
Endoplasmic Reticulum 2 2 1 1 0 1 1 4 3 2 1 2 4 0 0 9 5 7 1 1 3 3 4 4
Endosome 0 0 0 0 1 0 0 1 0 1 0 2 1 0 2 1 0 0 0 1 0 1 2 3
Golgi 0 0 0 0 1 0 2 0 0 0 0 0 0 1 1 1 0 1 1 0 0 0 0 0
Mitochondria 3 2 0 1 7 45 83 53 144 108 255 129 1 0 0 2 2 2 0 1 3 0 3 6
Nucleus 0 0 2 1 1 1 1 7 5 2 3 2 2 0 3 1 0 1 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 3 0 3 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 0 0 1 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 7 22 9 21 17 16 11 13 23 3 5 2 4 19 2 0 2 0 0 3 2
Unique Cell Count 243 235 191 316 597 476 552 562 496 470 588 537 514 669 773 320 218 354 239 237 191 420 566 716
Labelled Cell Count 246 242 199 331 621 512 612 601 582 537 711 614 530 687 793 366 240 395 239 237 191 420 566 716


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 9.8 8.4 8.7 7.8 6.0 5.6 5.8 4.8 5.2 4.5 4.9 9.9 9.8 9.3 17.9 17.9 18.3 9.6 9.2 9.3
Std Deviation (1e-4) 1.0 1.3 1.4 1.3 1.5 1.3 1.4 1.4 1.0 1.2 0.9 0.9 1.5 1.4 1.5 3.5 4.5 3.7 1.8 1.4 1.6
Intensity Change (Log2) 0.04 -0.11 -0.49 -0.59 -0.53 -0.8 -0.7 -0.91 -0.78 0.23 0.22 0.14 1.08 1.09 1.11 0.19 0.13 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.6 0 0 0 0 0 0 0 0 -0.4 0 0 3.4 1.2 3.3
Cytoplasm 0.4 -0.7 -1.5 -3.5 -3.1 -5.6 -4.6 -7.6 -5.5 1.3 3.2 2.0 0 -2.0 -0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.4 5.7 4.4 0 7.2 0 7.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.3 0 2.3 0 0 0 0 2.5 0 0 0 0 3.8 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.1856 6.6392 5.9159 5.6195 5.1158 5.7828 4.0107 6.0209 4.7799 4.1965 4.0891 4.849 4.1713 5.5595 4.7463 4.4523 4.4313 4.5942
Actin 0.0188 0.0007 0.0072 0.0035 0.0049 0.0014 0.022 0.0005 0.0123 0.009 0.0056 0.0054 0.0002 0.0001 0.0003 0 0.0001 0.0001
Bud 0.0021 0.0017 0.0041 0.0005 0.0006 0.0004 0.0002 0.0001 0.004 0.0004 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001
Bud Neck 0.007 0.0002 0.0041 0.0004 0.0009 0.001 0.0015 0.0001 0.0021 0.0005 0.0009 0.0006 0.0001 0.0001 0.0002 0.0002 0.0003 0.0004
Bud Periphery 0.0018 0.0006 0.0019 0.0003 0.0008 0.0007 0.0002 0 0.0017 0.0002 0.0003 0.0001 0 0 0 0 0 0
Bud Site 0.0064 0.0041 0.0092 0.001 0.0009 0.0002 0.0015 0.0004 0.006 0.0003 0.0004 0.0001 0.0002 0.0003 0.0002 0.0001 0.0001 0.0001
Cell Periphery 0.0003 0.0001 0.0007 0 0.0002 0.0003 0.0002 0 0.0003 0.0001 0.0001 0 0.0001 0 0 0 0 0
Cytoplasm 0.8043 0.9553 0.8936 0.8924 0.7712 0.9081 0.8377 0.9727 0.8934 0.8865 0.8387 0.9347 0.9587 0.9749 0.9753 0.9636 0.9617 0.9625
Cytoplasmic Foci 0.0276 0.0087 0.0155 0.0219 0.0345 0.018 0.0247 0.0054 0.0127 0.0136 0.0147 0.0122 0.0065 0.0072 0.0065 0.0094 0.0113 0.0096
Eisosomes 0.0002 0 0.0002 0 0.0001 0 0.0003 0 0.0002 0.0008 0.0003 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0091 0.001 0.0022 0.0029 0.0046 0.0031 0.0153 0.0022 0.004 0.0026 0.0048 0.005 0.009 0.0017 0.0025 0.0027 0.0018 0.004
Endosome 0.0303 0.0046 0.0159 0.01 0.05 0.0197 0.0252 0.0047 0.015 0.0214 0.0372 0.0208 0.0109 0.0082 0.0063 0.0154 0.0181 0.0146
Golgi 0.0094 0.0005 0.0037 0.002 0.017 0.0063 0.0053 0.0006 0.0049 0.0028 0.0065 0.0027 0.0011 0.0005 0.0022 0.0002 0.0004 0.001
Lipid Particles 0.0032 0.0001 0.0025 0.0003 0.0055 0.0036 0.0078 0 0.0078 0.0157 0.0138 0.0033 0.0002 0 0.0001 0 0 0
Mitochondria 0.0071 0.0003 0.0033 0.001 0.0429 0.0056 0.0058 0.0002 0.0085 0.0009 0.0334 0.001 0.0003 0.0002 0.0005 0.0002 0.0002 0.0007
None 0.048 0.0179 0.0201 0.0527 0.0175 0.0229 0.0323 0.0109 0.0103 0.0218 0.0217 0.006 0.0082 0.0038 0.0027 0.0026 0.0021 0.003
Nuclear Periphery 0.0034 0.0004 0.001 0.0009 0.0085 0.0006 0.0028 0.0003 0.0012 0.0016 0.0063 0.0023 0.0009 0.0003 0.0004 0.0006 0.0004 0.0005
Nucleolus 0.0005 0 0.0005 0.0001 0.0006 0.0001 0.0002 0 0.0037 0.0003 0.0008 0 0 0 0 0 0 0
Nucleus 0.0034 0.0014 0.0027 0.0022 0.0255 0.0012 0.0012 0.0006 0.0014 0.0013 0.0047 0.0008 0.0009 0.0007 0.0009 0.0011 0.0009 0.0011
Peroxisomes 0.0078 0.0003 0.0071 0.0036 0.0063 0.0037 0.0098 0 0.0064 0.0096 0.002 0.0019 0 0 0.0002 0 0.0001 0
Punctate Nuclear 0.003 0.0003 0.0011 0.0013 0.0023 0.0011 0.0029 0.0001 0.0011 0.003 0.0033 0.0013 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Vacuole 0.0044 0.0015 0.0026 0.0024 0.0032 0.0014 0.0024 0.001 0.0025 0.0064 0.0028 0.0011 0.002 0.0013 0.0011 0.003 0.0017 0.0015
Vacuole Periphery 0.0017 0.0002 0.0005 0.0005 0.0019 0.0005 0.0007 0.0002 0.0006 0.0014 0.0015 0.0006 0.0003 0.0003 0.0003 0.0007 0.0005 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.2993 45.04 44.785 27.3855 32.6624 38.2705 36.8873 22.4375 22.9431 32.3581
Translational Efficiency 1.3609 1.3863 0.9284 1.1992 1.153 0.8746 1.117 1.207 1.0119 1.1218

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
20 1100 2503 1749 1588 1940 343 1128 1608 3040 2846 2877

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 870.62 1086.33 1221.18 1311.40 926.76 1072.59 1363.75 1320.63 926.06 1077.56 1238.36 1315.02
Standard Deviation 141.79 164.31 156.69 190.03 139.95 149.49 172.90 173.12 140.11 155.16 165.38 183.64
Intensity Change Log 2 0.319347 0.488161 0.590993 0.210831 0.557312 0.510959 0.264414 0.524230 0.550280

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000416 0.000568 0.000143 0.000627 0.000209 0.000328 0.001081 0.000917 0.000212 0.000415 0.000256 0.000741
Bud Neck 0.004376 0.001471 0.000579 0.001985 0.001187 0.001080 0.002203 0.004782 0.001227 0.001222 0.000775 0.003082
Bud Site 0.024314 0.024572 0.004815 0.015879 0.010922 0.014375 0.025055 0.031625 0.011088 0.018065 0.007254 0.022052
Cell Periphery 0.000220 0.000178 0.000036 0.000194 0.000107 0.000082 0.000170 0.000110 0.000109 0.000117 0.000052 0.000161
Cytoplasm 0.411867* 0.782629* 0.896368* 0.880838* 0.756005* 0.794729* 0.699489* 0.627447* 0.751725* 0.790351* 0.872640* 0.781490*
Cytoplasmic Foci 0.355230* 0.130323 0.016572 0.023701 0.145715 0.122785 0.059271 0.060978 0.148321 0.125512 0.021718 0.038316
Eisosomes 0.000076 0.000014 0.000002 0.000005 0.000016 0.000012 0.000023 0.000011 0.000017 0.000013 0.000005 0.000007
Endoplasmic Reticulum 0.001627 0.001391 0.000116 0.000179 0.002842 0.000642 0.000682 0.000650 0.002826 0.000913 0.000185 0.000364
Endosome 0.021900 0.008478 0.000707 0.002226 0.007859 0.006118 0.005750 0.021699 0.008033 0.006972 0.001314 0.009861
Golgi 0.003576 0.007890 0.000179 0.002812 0.004515 0.007297 0.000415 0.014924 0.004503 0.007512 0.000207 0.007561
Lipid Particles 0.002980 0.000978 0.000279 0.001662 0.001363 0.000649 0.001568 0.003926 0.001383 0.000768 0.000435 0.002550
Mitochondria 0.000343 0.000414 0.000092 0.000248 0.001293 0.000990 0.000029 0.005143 0.001281 0.000782 0.000084 0.002167
Mitotic Spindle 0.000136 0.002179 0.001621 0.009939 0.002259 0.002316 0.005633 0.039288 0.002232 0.002266 0.002105 0.021446
None 0.000159 0.001469 0.003061 0.000798 0.001629 0.001231 0.002730 0.000852 0.001611 0.001317 0.003021 0.000819
Nuclear Periphery 0.000076 0.000019 0.000071 0.000049 0.000060 0.000022 0.000232 0.000605 0.000060 0.000021 0.000091 0.000267
Nuclear Periphery Foci 0.001507 0.001019 0.001086 0.001721 0.001075 0.000515 0.012343 0.004541 0.001080 0.000697 0.002442 0.002827
Nucleolus 0.003632 0.000092 0.000107 0.000237 0.000151 0.000132 0.000094 0.000482 0.000194 0.000118 0.000106 0.000333
Nucleus 0.059815 0.004792 0.042229 0.014930 0.013321 0.007473 0.032022 0.015265 0.013899 0.006503 0.040998 0.015061
Peroxisomes 0.002158 0.000966 0.000109 0.000935 0.000613 0.000796 0.000515 0.004242 0.000633 0.000858 0.000158 0.002231
Vacuole 0.103763 0.028873 0.031681 0.040435 0.046235 0.036275 0.150479 0.150766 0.046951 0.033597 0.045998 0.083693
Vacuole Periphery 0.001827 0.001684 0.000146 0.000601 0.002625 0.002150 0.000217 0.011747 0.002615 0.001982 0.000155 0.004971

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.72 1.42 -1.00 -0.03 -2.47 -1.64 -3.03 -1.51 -0.76 1.24 -2.40 -0.76 -3.13 -0.95 -1.99
Bud Neck 1.85 2.40 1.02 -2.29 -4.52 1.13 -7.01 -6.53 -6.77 -2.85 0.06 3.46 -6.18 -5.93 -7.15
Bud Site -0.05 2.36 1.03 4.58 -8.63 -2.87 -3.30 -4.91 -3.45 -0.30 -5.82 3.82 -4.99 0.03 -9.44
Cell Periphery 0.68 3.08 0.94 0.64 -8.28 4.00 -5.85 3.37 0.52 7.82 -0.90 8.92 -1.94 -1.11 -7.56
Cytoplasm -7.04 -8.78 -7.89 -5.13 7.90 -5.24 9.70 14.70 18.92 0.51 -5.62 -13.37 1.92 8.21 16.68
Cytoplasmic Foci 4.46 6.71 6.52 21.44 -5.69 4.36 16.11 19.84 16.36 0.71 4.69 30.64 26.18 28.54 -9.05
Eisosomes 3.98 4.72 4.56 5.13 -7.09 3.72 -5.29 6.17 2.38 9.41 3.39 11.45 10.10 6.76 -3.14
Endoplasmic Reticulum 0.35 2.95 2.91 2.88 -0.61 5.42 5.50 6.57 2.15 4.74 4.59 6.96 6.93 3.83 -0.26
Endosome 2.01 3.22 3.04 6.79 -3.78 2.83 4.64 -2.34 -3.73 -4.61 1.70 14.40 3.72 2.25 -7.95
Golgi -2.72 2.48 1.25 6.05 -2.73 -3.46 12.37 -0.53 1.42 -3.83 -4.62 13.04 2.09 5.40 -4.79
Lipid Particles 2.68 3.56 1.17 -4.31 -7.80 5.80 -2.87 -1.93 -3.09 -1.13 4.80 6.96 -2.97 -5.30 -6.14
Mitochondria -0.29 1.23 0.83 1.46 -1.32 0.48 2.31 -0.48 -0.89 -2.13 0.86 2.23 0.78 -0.03 -2.21
Mitotic Spindle -2.12 -4.24 -5.64 -3.75 -4.50 -0.06 -1.05 -7.75 -7.91 -5.52 -0.07 -0.04 -7.93 -8.62 -8.64
None -2.43 -14.37 -5.86 1.26 11.30 0.99 -0.35 3.24 1.14 2.01 0.82 -3.77 3.48 1.73 11.30
Nuclear Periphery 2.73 -1.72 -1.32 -10.32 0.80 3.39 -10.70 -6.87 -7.66 -1.03 3.60 -6.91 -7.19 -9.60 -4.10
Nuclear Periphery Foci 1.13 0.25 -2.04 -6.47 -4.71 4.34 -11.52 -6.15 -7.79 8.79 3.11 -8.79 -8.15 -10.84 -0.15
Nucleolus 1.01 1.00 0.93 -1.98 -1.79 0.41 3.56 -2.44 -2.47 -2.82 1.44 1.70 -1.92 -2.86 -3.00
Nucleus 1.18 0.20 0.81 -19.58 19.16 7.90 -10.17 -6.25 -12.39 6.52 8.17 -24.73 -7.05 -21.85 21.04
Peroxisomes 2.62 4.68 2.87 0.81 -4.20 -1.87 2.05 -4.58 -4.14 -5.10 -2.40 6.36 -4.09 -3.05 -7.04
Vacuole 3.45 2.63 0.93 -14.97 -10.01 4.07 -15.63 -25.94 -27.70 0.48 5.88 -7.42 -23.43 -28.75 -16.42
Vacuole Periphery 0.20 2.75 2.30 7.91 -3.39 1.19 8.18 -1.46 -1.95 -4.06 1.84 8.39 1.59 0.30 -4.47