Standard name
Description Dual specificity protein phosphatase; regulates growth, sporulation, and glycogen accumulation in a cAMP-dependent protein kinase cascade dependent manner; mutants are defective in 60S ribosome assembly; positively regulates pre-autophagosomal structure (PAS) formation upon nitrogen starvation or rapamycin treatment

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.06 0.12 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.97 0.97 0.96 0.94 0.88 0.90 0.80 0.84 0.70 0.80 0.98 1.00 0.99 0.97 0.92 0.96 0.95 0.97 0.94 0.97 0.97 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.15 0.09 0.29 0.23 0.43 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 0 1 1 1 0 0 0 0 0 0 0 1 0 0 1 0 1
Bud 0 2 4 0 2 0 7 7 6 9 20 12 0 2 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 4 6 13 14 7 7 8 8 7 7 12 13 13 19 38 12 41 0 0 0 0 0 0
Cytoplasm 239 232 185 308 571 448 486 503 395 394 411 430 506 666 764 310 201 341 228 229 180 405 546 688
Endoplasmic Reticulum 2 2 1 1 0 1 1 4 3 2 1 2 4 0 0 9 5 7 1 1 3 3 4 4
Endosome 0 0 0 0 1 0 0 1 0 1 0 2 1 0 2 1 0 0 0 1 0 1 2 3
Golgi 0 0 0 0 1 0 2 0 0 0 0 0 0 1 1 1 0 1 1 0 0 0 0 0
Mitochondria 3 2 0 1 7 45 83 53 144 108 255 129 1 0 0 2 2 2 0 1 3 0 3 6
Nucleus 0 0 2 1 1 1 1 7 5 2 3 2 2 0 3 1 0 1 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 3 0 3 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 0 0 1 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 7 22 9 21 17 16 11 13 23 3 5 2 4 19 2 0 2 0 0 3 2
Unique Cell Count 243 235 191 316 597 476 552 562 496 470 588 537 514 669 773 320 218 354 239 237 191 420 566 716
Labelled Cell Count 246 242 199 331 621 512 612 601 582 537 711 614 530 687 793 366 240 395 239 237 191 420 566 716

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 9.8 8.4 8.7 7.8 6.0 5.6 5.8 4.8 5.2 4.5 4.9 9.9 9.8 9.3 17.9 17.9 18.3 9.6 9.2 9.3
Std Deviation (1e-4) 1.0 1.3 1.4 1.3 1.5 1.3 1.4 1.4 1.0 1.2 0.9 0.9 1.5 1.4 1.5 3.5 4.5 3.7 1.8 1.4 1.6
Intensity Change (Log2) 0.04 -0.11 -0.49 -0.59 -0.53 -0.8 -0.7 -0.91 -0.78 0.23 0.22 0.14 1.08 1.09 1.11 0.19 0.13 0.14

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.6 0 0 0 0 0 0 0 0 -0.4 0 0 3.4 1.2 3.3
Cytoplasm 1.8 -4.6 -5.7 -9.3 -9.7 0 -9.7 0 -9.2 -7.1 -10.1 -9.4 6.6 0.9 6.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.4 5.7 4.4 0 7.2 0 7.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 2.2 0 0 0 0 0 0