Standard name | |
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Description | Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 2 | 3 | 1 | 4 | 1 | – | 10 | 6 | 10 | 4 | 4 | 4 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 9 | 12 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 0 | – | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 0 | 1 | 1 | – | 3 | 2 | 1 | 1 | 4 | 0 | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 257 | 85 | 84 | 156 | 361 | 242 | – | 407 | 235 | 255 | 187 | 328 | 344 | 452 | 585 | 112 | 22 | 78 | 253 | 81 | 79 | 71 | 267 | 346 |
Endoplasmic Reticulum | 4 | 0 | 0 | 0 | 1 | 0 | – | 1 | 2 | 1 | 0 | 5 | 3 | 2 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 5 |
Endosome | 1 | 0 | 0 | 0 | 0 | 2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 3 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 |
Mitochondria | 5 | 4 | 1 | 20 | 21 | 66 | – | 145 | 146 | 139 | 123 | 233 | 7 | 5 | 4 | 1 | 1 | 1 | 2 | 3 | 2 | 0 | 1 | 6 |
Nucleus | 2 | 0 | 0 | 1 | 2 | 5 | – | 25 | 14 | 12 | 9 | 24 | 4 | 1 | 3 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 1 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 1 | 1 | 0 | – | 2 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 19 | 12 | – | 47 | 39 | 50 | 25 | 26 | 4 | 6 | 27 | 2 | 1 | 5 | 6 | 0 | 1 | 1 | 3 | 14 |
Unique Cell Count | 266 | 88 | 84 | 160 | 386 | 288 | 548 | 340 | 390 | 281 | 523 | 359 | 460 | 611 | 113 | 23 | 83 | 273 | 90 | 86 | 77 | 296 | 405 | |
Labelled Cell Count | 270 | 94 | 90 | 179 | 410 | 329 | 642 | 447 | 471 | 352 | 629 | 367 | 472 | 621 | 119 | 27 | 86 | 273 | 90 | 86 | 77 | 296 | 405 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 5.6 | 4.2 | 4.3 | 5.6 | 5.0 | – | 4.7 | 4.5 | 4.8 | 4.6 | 4.7 | 6.7 | 8.5 | 8.4 | 22.4 | 23.8 | 21.4 | 4.9 | 5.7 | 6.2 |
Std Deviation (1e-4) | 1.1 | 1.1 | 0.7 | 1.4 | 1.9 | 1.3 | – | 1.5 | 1.3 | 1.4 | 1.5 | 1.4 | 2.8 | 3.3 | 3.5 | 8.5 | 7.8 | 10.4 | 1.2 | 2.0 | 2.6 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.41 | 0.25 | – | 0.17 | 0.1 | 0.2 | 0.14 | 0.16 | 0.68 | 1.02 | 1.01 | 2.41 | 2.5 | 2.35 | 0.21 | 0.44 | 0.56 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.5 | -2.4 | -3.9 | – | -5.3 | -5.9 | -6.4 | -6.2 | -6.8 | -1.9 | -1.2 | -1.9 | -0.9 | -1.9 | -2.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.6 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 2.8 | 3.3 | 3.5 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.5791 | 3.0258 | 2.4808 | 2.1026 | 2.0447 | 2.7797 | 1.4846 | 2.5522 | 1.9782 | 1.8556 | 1.9456 | 1.9708 | 3.7589 | 5.0777 | 4.3177 | 3.8544 | 5.3546 | 4.3329 |
Actin | 0.0179 | 0.0011 | 0.0162 | 0.0241 | 0.0192 | 0.0333 | 0.0132 | 0.0005 | 0.0068 | 0.0039 | 0.0032 | 0.0002 | 0.0721 | 0.0041 | 0.0062 | 0.0433 | 0.0488 | 0.0047 |
Bud | 0.0004 | 0.0041 | 0.0039 | 0.0019 | 0.0014 | 0.0012 | 0.0005 | 0.0009 | 0.0052 | 0.0074 | 0.0046 | 0.0001 | 0.0013 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0004 |
Bud Neck | 0.0009 | 0.0003 | 0.0011 | 0.0054 | 0.0019 | 0.0017 | 0.0054 | 0.0002 | 0.0007 | 0.0004 | 0.0006 | 0.0009 | 0.0057 | 0.0002 | 0.0004 | 0.0012 | 0.0005 | 0.0008 |
Bud Periphery | 0.0005 | 0.0023 | 0.0033 | 0.0023 | 0.0023 | 0.0016 | 0.0004 | 0.0002 | 0.0025 | 0.0017 | 0.0052 | 0 | 0.0016 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0004 |
Bud Site | 0.0066 | 0.0147 | 0.0193 | 0.0032 | 0.0057 | 0.0004 | 0.015 | 0.0015 | 0.0029 | 0.0021 | 0.0003 | 0 | 0.0175 | 0.001 | 0.0009 | 0.0007 | 0.0003 | 0.0004 |
Cell Periphery | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.6375 | 0.8803 | 0.7345 | 0.762 | 0.7382 | 0.8329 | 0.7045 | 0.8558 | 0.8033 | 0.6915 | 0.8361 | 0.887 | 0.5802 | 0.9035 | 0.8119 | 0.8037 | 0.7468 | 0.8218 |
Cytoplasmic Foci | 0.031 | 0.0064 | 0.0264 | 0.0348 | 0.0409 | 0.0091 | 0.0448 | 0.0046 | 0.0191 | 0.0192 | 0.0128 | 0.008 | 0.0552 | 0.0074 | 0.014 | 0.0167 | 0.0079 | 0.0129 |
Eisosomes | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0006 | 0.0003 | 0 | 0.0003 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0046 | 0.0018 | 0.0039 | 0.0124 | 0.0041 | 0.0022 | 0.0025 | 0.0031 | 0.0032 | 0.0031 | 0.002 | 0.0015 | 0.009 | 0.0014 | 0.0032 | 0.0024 | 0.0033 | 0.0041 |
Endosome | 0.0198 | 0.0016 | 0.0241 | 0.0248 | 0.08 | 0.0059 | 0.0188 | 0.0017 | 0.0155 | 0.048 | 0.0082 | 0.0026 | 0.0193 | 0.0023 | 0.0042 | 0.0053 | 0.0083 | 0.0123 |
Golgi | 0.0053 | 0.0004 | 0.016 | 0.0157 | 0.0279 | 0.0022 | 0.0066 | 0.0001 | 0.0047 | 0.0098 | 0.0055 | 0.0001 | 0.0115 | 0.0006 | 0.0029 | 0.0019 | 0.0083 | 0.0086 |
Lipid Particles | 0.0107 | 0.0003 | 0.0089 | 0.0019 | 0.0136 | 0.0065 | 0.0036 | 0.0002 | 0.0023 | 0.0094 | 0.0142 | 0.0001 | 0.0152 | 0.0009 | 0.0104 | 0.0201 | 0.0076 | 0.0103 |
Mitochondria | 0.0056 | 0.0004 | 0.018 | 0.0129 | 0.0202 | 0.0017 | 0.0011 | 0.0002 | 0.0083 | 0.048 | 0.0136 | 0.0001 | 0.0072 | 0.0003 | 0.0014 | 0.0006 | 0.0016 | 0.0095 |
None | 0.2174 | 0.0745 | 0.0978 | 0.0653 | 0.0198 | 0.0809 | 0.1404 | 0.1197 | 0.1027 | 0.1313 | 0.0554 | 0.0862 | 0.1247 | 0.0713 | 0.1236 | 0.0825 | 0.064 | 0.094 |
Nuclear Periphery | 0.0096 | 0.0006 | 0.0024 | 0.0073 | 0.0022 | 0.0026 | 0.0028 | 0.0013 | 0.0023 | 0.0028 | 0.0011 | 0.001 | 0.01 | 0.0006 | 0.0019 | 0.0021 | 0.0426 | 0.0018 |
Nucleolus | 0.0011 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.0002 | 0.0006 | 0.0002 | 0.0002 | 0.0014 | 0.0005 | 0 | 0.0006 | 0 | 0.0008 | 0.0004 | 0.0002 | 0.0001 |
Nucleus | 0.0072 | 0.0057 | 0.0078 | 0.0067 | 0.0044 | 0.0085 | 0.0078 | 0.0072 | 0.0089 | 0.0063 | 0.0058 | 0.0104 | 0.0177 | 0.002 | 0.0077 | 0.0071 | 0.0478 | 0.0105 |
Peroxisomes | 0.0092 | 0.004 | 0.01 | 0.0132 | 0.0087 | 0.003 | 0.0176 | 0.0001 | 0.0057 | 0.0058 | 0.0251 | 0.0001 | 0.0401 | 0.002 | 0.0055 | 0.0041 | 0.0071 | 0.005 |
Punctate Nuclear | 0.0112 | 0.0008 | 0.0026 | 0.0023 | 0.0029 | 0.0052 | 0.012 | 0.0012 | 0.0035 | 0.0032 | 0.0007 | 0.001 | 0.0064 | 0.0004 | 0.0036 | 0.0066 | 0.0026 | 0.0016 |
Vacuole | 0.002 | 0.0005 | 0.002 | 0.0024 | 0.0047 | 0.0006 | 0.002 | 0.0012 | 0.0016 | 0.0028 | 0.0036 | 0.0006 | 0.0021 | 0.0007 | 0.0006 | 0.0006 | 0.0007 | 0.0006 |
Vacuole Periphery | 0.001 | 0.0001 | 0.0008 | 0.0007 | 0.0011 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.0014 | 0.0011 | 0.0001 | 0.0013 | 0.0001 | 0.0001 | 0.0002 | 0.0009 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.6203 | 48.8051 | 20.9669 | 13.2949 | 7.4511 | 5.7437 | 14.9339 | 14.8999 | 10.0652 | 4.8885 |
Translational Efficiency | 2.4297 | 1.047 | 1.7615 | 2.4019 | 3.5518 | 3.5156 | 1.9614 | 2.6963 | 2.422 | 1.9281 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |