Standard name
Description Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0
Cytoplasm 0.97 0.97 1.0 0.98 0.94 0.84 – 0.74 0.69 0.65 0.67 0.63 0.96 0.98 0.96 0.99 0.96 0.94 0.93 0.9 0.92 0.93 0.9 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0.12 0.05 0.23 – 0.26 0.43 0.36 0.44 0.45 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 – 0.09 0.11 0.13 0.09 0.05 0 0 0 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1
Bud 0 2 3 1 4 1 – 10 6 10 4 4 4 2 0 0 0 0 1 0 0 0 9 12
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 1 0 0 0 – 1 0 1 1 1 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 1 0 1 1 – 3 2 1 1 4 0 2 0 0 2 1 0 0 0 0 0 0
Cytoplasm 257 85 84 156 361 242 – 407 235 255 187 328 344 452 585 112 22 78 253 81 79 71 267 346
Endoplasmic Reticulum 4 0 0 0 1 0 – 1 2 1 0 5 3 2 2 2 1 0 0 0 0 0 3 5
Endosome 1 0 0 0 0 2 – 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 3 3
Golgi 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 2
Mitochondria 5 4 1 20 21 66 – 145 146 139 123 233 7 5 4 1 1 1 2 3 2 0 1 6
Nucleus 2 0 0 1 2 5 – 25 14 12 9 24 4 1 3 2 0 1 0 0 0 0 0 1
Nuclear Periphery 1 0 0 0 0 0 – 0 2 1 2 2 0 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 1 1 0 – 2 1 0 0 2 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Vac/Vac Membrane 0 1 0 0 19 12 – 47 39 50 25 26 4 6 27 2 1 5 6 0 1 1 3 14
Unique Cell Count 266 88 84 160 386 288 548 340 390 281 523 359 460 611 113 23 83 273 90 86 77 296 405
Labelled Cell Count 270 94 90 179 410 329 642 447 471 352 629 367 472 621 119 27 86 273 90 86 77 296 405


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 5.6 4.2 4.3 5.6 5.0 – 4.7 4.5 4.8 4.6 4.7 6.7 8.5 8.4 22.4 23.8 21.4 4.9 5.7 6.2
Std Deviation (1e-4) 1.1 1.1 0.7 1.4 1.9 1.3 – 1.5 1.3 1.4 1.5 1.4 2.8 3.3 3.5 8.5 7.8 10.4 1.2 2.0 2.6
Intensity Change (Log2) – – – 0.03 0.41 0.25 – 0.17 0.1 0.2 0.14 0.16 0.68 1.02 1.01 2.41 2.5 2.35 0.21 0.44 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.5 -2.4 -3.9 – -5.3 -5.9 -6.4 -6.2 -6.8 -1.9 -1.2 -1.9 -0.9 -1.9 -2.3
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.6 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 2.8 3.3 3.5 2.8 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5791 3.0258 2.4808 2.1026 2.0447 2.7797 1.4846 2.5522 1.9782 1.8556 1.9456 1.9708 3.7589 5.0777 4.3177 3.8544 5.3546 4.3329
Actin 0.0179 0.0011 0.0162 0.0241 0.0192 0.0333 0.0132 0.0005 0.0068 0.0039 0.0032 0.0002 0.0721 0.0041 0.0062 0.0433 0.0488 0.0047
Bud 0.0004 0.0041 0.0039 0.0019 0.0014 0.0012 0.0005 0.0009 0.0052 0.0074 0.0046 0.0001 0.0013 0.0004 0.0003 0.0002 0.0002 0.0004
Bud Neck 0.0009 0.0003 0.0011 0.0054 0.0019 0.0017 0.0054 0.0002 0.0007 0.0004 0.0006 0.0009 0.0057 0.0002 0.0004 0.0012 0.0005 0.0008
Bud Periphery 0.0005 0.0023 0.0033 0.0023 0.0023 0.0016 0.0004 0.0002 0.0025 0.0017 0.0052 0 0.0016 0.0002 0.0001 0.0001 0.0002 0.0004
Bud Site 0.0066 0.0147 0.0193 0.0032 0.0057 0.0004 0.015 0.0015 0.0029 0.0021 0.0003 0 0.0175 0.001 0.0009 0.0007 0.0003 0.0004
Cell Periphery 0.0001 0.0001 0.0003 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001 0.0004 0.0003 0 0.0005 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.6375 0.8803 0.7345 0.762 0.7382 0.8329 0.7045 0.8558 0.8033 0.6915 0.8361 0.887 0.5802 0.9035 0.8119 0.8037 0.7468 0.8218
Cytoplasmic Foci 0.031 0.0064 0.0264 0.0348 0.0409 0.0091 0.0448 0.0046 0.0191 0.0192 0.0128 0.008 0.0552 0.0074 0.014 0.0167 0.0079 0.0129
Eisosomes 0.0002 0 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001 0 0 0.0006 0.0003 0 0.0003 0.0001 0
Endoplasmic Reticulum 0.0046 0.0018 0.0039 0.0124 0.0041 0.0022 0.0025 0.0031 0.0032 0.0031 0.002 0.0015 0.009 0.0014 0.0032 0.0024 0.0033 0.0041
Endosome 0.0198 0.0016 0.0241 0.0248 0.08 0.0059 0.0188 0.0017 0.0155 0.048 0.0082 0.0026 0.0193 0.0023 0.0042 0.0053 0.0083 0.0123
Golgi 0.0053 0.0004 0.016 0.0157 0.0279 0.0022 0.0066 0.0001 0.0047 0.0098 0.0055 0.0001 0.0115 0.0006 0.0029 0.0019 0.0083 0.0086
Lipid Particles 0.0107 0.0003 0.0089 0.0019 0.0136 0.0065 0.0036 0.0002 0.0023 0.0094 0.0142 0.0001 0.0152 0.0009 0.0104 0.0201 0.0076 0.0103
Mitochondria 0.0056 0.0004 0.018 0.0129 0.0202 0.0017 0.0011 0.0002 0.0083 0.048 0.0136 0.0001 0.0072 0.0003 0.0014 0.0006 0.0016 0.0095
None 0.2174 0.0745 0.0978 0.0653 0.0198 0.0809 0.1404 0.1197 0.1027 0.1313 0.0554 0.0862 0.1247 0.0713 0.1236 0.0825 0.064 0.094
Nuclear Periphery 0.0096 0.0006 0.0024 0.0073 0.0022 0.0026 0.0028 0.0013 0.0023 0.0028 0.0011 0.001 0.01 0.0006 0.0019 0.0021 0.0426 0.0018
Nucleolus 0.0011 0.0001 0.0004 0.0002 0.0005 0.0002 0.0006 0.0002 0.0002 0.0014 0.0005 0 0.0006 0 0.0008 0.0004 0.0002 0.0001
Nucleus 0.0072 0.0057 0.0078 0.0067 0.0044 0.0085 0.0078 0.0072 0.0089 0.0063 0.0058 0.0104 0.0177 0.002 0.0077 0.0071 0.0478 0.0105
Peroxisomes 0.0092 0.004 0.01 0.0132 0.0087 0.003 0.0176 0.0001 0.0057 0.0058 0.0251 0.0001 0.0401 0.002 0.0055 0.0041 0.0071 0.005
Punctate Nuclear 0.0112 0.0008 0.0026 0.0023 0.0029 0.0052 0.012 0.0012 0.0035 0.0032 0.0007 0.001 0.0064 0.0004 0.0036 0.0066 0.0026 0.0016
Vacuole 0.002 0.0005 0.002 0.0024 0.0047 0.0006 0.002 0.0012 0.0016 0.0028 0.0036 0.0006 0.0021 0.0007 0.0006 0.0006 0.0007 0.0006
Vacuole Periphery 0.001 0.0001 0.0008 0.0007 0.0011 0.0002 0.0003 0.0001 0.0004 0.0014 0.0011 0.0001 0.0013 0.0001 0.0001 0.0002 0.0009 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.6203 48.8051 20.9669 13.2949 7.4511 5.7437 14.9339 14.8999 10.0652 4.8885
Translational Efficiency 2.4297 1.047 1.7615 2.4019 3.5518 3.5156 1.9614 2.6963 2.422 1.9281

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘