Standard name
Human Ortholog
Description Predominantly nucleolar DEAH-box ATP-dependent RNA helicase; required for 18S rRNA synthesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.05 0 0.07 0.13 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0 0.1 0.05 0.07 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.05 0.07 0.05 0.07 0 0.1 0.07 0.08 0 0.09 0.14 0 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0.07 0.11 0 0.07 0.05 0.05 0.06 0.05 0.06 0.08 0.12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.4 0.25 0.07 0.11 0.05 0.28 0.29 0.15 0.7 0.54 0.68 0.41 0.14 0 0 0.06 0 0.06 0 0 0 0 0 0
Nucleus 0.38 0.43 0.65 0.51 0.45 0.26 0.28 0.22 0.2 0.18 0.21 0.2 0.38 0.27 0.24 0.32 0.4 0.32 0.18 0.2 0.19 0.18 0.05 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.62 0.48 0.08 0.45 0.23 0.31 0.16 0.15 0.18 0.16 0.14 0.11 0.65 0.67 0.62 0.63 0.57 0.6 0.45 0.48 0.53 0.49 0.47 0.42
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.17 0.24 0.44 0.41 0.48 0.41 0.5 0.6 0.31 0.32 0.25 0.44 0.19 0.37 0.27 0.13 0.19 0.21 0.23 0.13 0.1 0.13 0.27 0.27
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0.08 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 2
Bud 0 1 1 1 1 4 9 14 8 15 13 14 0 0 2 0 0 0 1 4 1 1 8 3
Bud Neck 0 2 7 2 10 2 21 42 4 14 8 24 0 6 3 1 0 0 1 3 4 2 15 14
Bud Site 1 1 0 1 4 7 16 6 31 18 20 23 1 1 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 3 2 5 9 10 17 15 23 13 32 21 27 11 45 81 2 4 7 0 0 2 1 1 2
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 0 2 0 0 0 1 2 0 2 0 0 0 2 2 2
Endosome 0 6 0 1 6 20 10 12 0 4 7 7 8 35 62 4 8 7 5 9 10 12 38 53
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 1 1 0 0 1 5 2
Mitochondria 32 34 11 16 11 71 93 49 227 179 206 134 35 20 25 8 0 8 0 1 1 3 3 4
Nucleus 30 58 104 78 91 65 90 71 65 60 63 67 95 129 140 43 49 42 16 30 37 38 27 23
Nuclear Periphery 2 0 0 0 1 0 0 1 0 0 0 0 1 0 1 3 0 1 0 1 1 0 1 2
Nucleolus 50 64 13 68 46 77 52 48 58 52 43 37 164 323 354 85 69 80 42 73 101 102 244 190
Peroxisomes 0 1 0 0 0 0 0 2 0 1 0 0 0 3 5 0 0 2 0 0 0 0 4 1
SpindlePole 14 32 71 63 98 103 159 194 102 106 77 144 48 179 157 18 23 28 21 20 19 26 138 125
Vac/Vac Membrane 0 3 0 1 3 5 4 7 2 3 7 6 2 1 4 6 6 3 2 5 9 16 18 21
Unique Cell Count 80 134 160 152 204 252 321 324 324 329 303 328 252 480 573 134 121 133 95 151 192 210 518 459
Labelled Cell Count 132 204 213 240 281 371 469 470 511 485 467 483 365 742 838 174 159 181 95 151 192 210 518 459


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 4.8 5.3 5.2 5.7 4.3 4.3 4.6 3.5 3.6 3.4 3.8 5.5 5.4 5.3 9.0 10.1 9.3 5.1 5.1 5.4
Std Deviation (1e-4) 0.6 0.7 1.0 1.3 1.4 1.2 0.8 1.2 0.7 0.7 0.8 0.7 1.2 1.1 1.2 2.5 2.5 2.3 1.1 1.2 1.4
Intensity Change (Log2) – – – -0.02 0.09 -0.32 -0.29 -0.2 -0.59 -0.58 -0.64 -0.47 0.05 0.02 0.01 0.77 0.94 0.82 -0.06 -0.05 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5519 0.8234 0.436 0.8201 0.2774 0.5967 0.9362 1.424 1.0885 0.6928 1.0096 1.1034 1.7322 2.0221 1.4556 1.4858 1.4791 1.4866
Actin 0.0124 0 0.0001 0.0033 0 0.0039 0.0085 0 0.0006 0 0.0005 0.0001 0.0518 0 0.0029 0 0.0431 0.0068
Bud 0.0003 0.0001 0 0.0066 0 0.0004 0.0004 0 0.0009 0 0.0003 0 0.0004 0.0001 0.0001 0.0001 0.0008 0.0001
Bud Neck 0.0049 0.0012 0.0013 0.0024 0.0013 0.0027 0.0039 0.0004 0.0005 0.0006 0.0009 0.0007 0.0029 0.0014 0.0007 0.0015 0.0013 0.0012
Bud Periphery 0.0006 0.0003 0.0001 0.0175 0.0003 0.0009 0.0006 0.0001 0.0016 0.0001 0.0007 0.0001 0.0006 0.0002 0.0002 0.0004 0.0017 0.0001
Bud Site 0.003 0.001 0.0008 0.0216 0.0002 0.0009 0.0055 0.0003 0.0009 0.0002 0.0004 0.0002 0.0055 0.0019 0.0012 0.001 0.0019 0.0005
Cell Periphery 0.0003 0.0002 0.0002 0.0012 0.0002 0.0002 0.0007 0.0001 0.0002 0.0001 0.0005 0.0001 0.0005 0.0005 0.0003 0.0009 0.0003 0.0001
Cytoplasm 0.0044 0.0004 0.0013 0.0009 0 0.0003 0.0044 0 0.0006 0 0.0001 0 0.0142 0 0.0001 0 0.0002 0.0002
Cytoplasmic Foci 0.0136 0.001 0.0068 0.0106 0.0006 0.0027 0.0055 0.0004 0.0087 0.0003 0.0111 0.0011 0.0197 0.0005 0.0014 0.005 0.0076 0.0035
Eisosomes 0.0001 0 0.0001 0.0001 0 0 0.0001 0 0 0 0.0001 0 0.0006 0 0.0001 0 0.0002 0.0001
Endoplasmic Reticulum 0.0085 0.0001 0.0003 0.0006 0 0.0004 0.003 0 0.0002 0 0.0006 0 0.0016 0 0.0001 0 0.0005 0.0002
Endosome 0.0146 0.0016 0.002 0.0174 0.0002 0.0015 0.0146 0.0001 0.0129 0 0.0153 0.0028 0.0135 0.0002 0.0022 0.0016 0.0272 0.0026
Golgi 0.0033 0.0002 0.0006 0.0056 0.0002 0.0006 0.0089 0.0001 0.0064 0 0.0061 0.0035 0.0174 0.0001 0.001 0.0007 0.0155 0.0024
Lipid Particles 0.0283 0.0046 0.0308 0.0196 0.0053 0.0121 0.0178 0.0021 0.0126 0.0015 0.145 0.0082 0.0444 0.0023 0.0045 0.037 0.0298 0.0087
Mitochondria 0.0013 0.0007 0.0003 0.0125 0.0006 0.0007 0.0108 0.0001 0.0113 0.0001 0.0922 0.001 0.0054 0.0002 0.0101 0.0056 0.0118 0.0146
None 0.003 0.0019 0.0042 0.0014 0.0001 0.0002 0.0013 0 0.0001 0 0.0002 0 0.0144 0 0 0.0001 0.0002 0.0013
Nuclear Periphery 0.0234 0.0031 0.0039 0.0008 0.0007 0.0016 0.0135 0.0001 0.0002 0 0.0032 0.0001 0.0064 0.0001 0.0001 0.0002 0.001 0.0002
Nucleolus 0.6622 0.7897 0.8258 0.7635 0.7339 0.811 0.7764 0.839 0.8542 0.9087 0.5043 0.8874 0.672 0.8738 0.8576 0.8231 0.644 0.8028
Nucleus 0.1342 0.142 0.0836 0.072 0.1381 0.0854 0.0963 0.1468 0.0773 0.0849 0.0853 0.0812 0.0806 0.1062 0.1036 0.1108 0.1355 0.1082
Peroxisomes 0.0061 0.0003 0.0009 0.0026 0.0003 0.0006 0.0018 0.0001 0.0045 0.0001 0.0056 0.0003 0.0143 0.0001 0.0003 0.0013 0.0028 0.0033
Punctate Nuclear 0.0722 0.0485 0.0357 0.0298 0.1166 0.0728 0.0205 0.0096 0.0027 0.0029 0.1101 0.0125 0.0313 0.0096 0.0116 0.0024 0.0685 0.0419
Vacuole 0.0022 0.0025 0.0011 0.008 0.0008 0.0009 0.0034 0.0007 0.0024 0.0003 0.0062 0.0004 0.0017 0.0026 0.0017 0.0054 0.0038 0.0006
Vacuole Periphery 0.001 0.0005 0.0003 0.0021 0.0005 0.0002 0.002 0.0001 0.0011 0 0.0116 0.0002 0.0008 0.0002 0.0002 0.0027 0.0021 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.735 26.357 23.5359 11.6731 20.0856 12.9858 16.8253 8.8409 7.0019 15.7018
Translational Efficiency 0.7662 0.7503 0.6285 0.6753 0.6355 0.6618 0.7054 0.7041 0.6742 0.6998

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Predominantly nucleolar DEAH-box ATP-dependent RNA helicase; required for 18S rRNA synthesis
Localization
Cell Percentages nucleolus (79%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-1

Dhr2

Dhr2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dhr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available