Standard name
Description Predominantly nucleolar DEAH-box ATP-dependent RNA helicase; required for 18S rRNA synthesis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.05 0 0.07 0.13 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0 0.1 0.05 0.07 0.07 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.05 0.07 0.05 0.07 0 0.1 0.07 0.08 0 0.09 0.14 0 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0.07 0.11 0 0.07 0.05 0.05 0.06 0.05 0.06 0.08 0.12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.4 0.25 0.07 0.11 0.05 0.28 0.29 0.15 0.7 0.54 0.68 0.41 0.14 0 0 0.06 0 0.06 0 0 0 0 0 0
Nucleus 0.38 0.43 0.65 0.51 0.45 0.26 0.28 0.22 0.2 0.18 0.21 0.2 0.38 0.27 0.24 0.32 0.4 0.32 0.18 0.2 0.19 0.18 0.05 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.62 0.48 0.08 0.45 0.23 0.31 0.16 0.15 0.18 0.16 0.14 0.11 0.65 0.67 0.62 0.63 0.57 0.6 0.45 0.48 0.53 0.49 0.47 0.42
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.17 0.24 0.44 0.41 0.48 0.41 0.5 0.6 0.31 0.32 0.25 0.44 0.19 0.37 0.27 0.13 0.19 0.21 0.23 0.13 0.1 0.13 0.27 0.27
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0.08 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 2
Bud 0 1 1 1 1 4 9 14 8 15 13 14 0 0 2 0 0 0 1 4 1 1 8 3
Bud Neck 0 2 7 2 10 2 21 42 4 14 8 24 0 6 3 1 0 0 1 3 4 2 15 14
Bud Site 1 1 0 1 4 7 16 6 31 18 20 23 1 1 1 0 0 0
Cell Periphery 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 3 2 5 9 10 17 15 23 13 32 21 27 11 45 81 2 4 7 0 0 2 1 1 2
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 0 2 0 0 0 1 2 0 2 0 0 0 2 2 2
Endosome 0 6 0 1 6 20 10 12 0 4 7 7 8 35 62 4 8 7 5 9 10 12 38 53
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 1 1 0 0 1 5 2
Mitochondria 32 34 11 16 11 71 93 49 227 179 206 134 35 20 25 8 0 8 0 1 1 3 3 4
Nucleus 30 58 104 78 91 65 90 71 65 60 63 67 95 129 140 43 49 42 16 30 37 38 27 23
Nuclear Periphery 2 0 0 0 1 0 0 1 0 0 0 0 1 0 1 3 0 1 0 1 1 0 1 2
Nucleolus 50 64 13 68 46 77 52 48 58 52 43 37 164 323 354 85 69 80 42 73 101 102 244 190
Peroxisomes 0 1 0 0 0 0 0 2 0 1 0 0 0 3 5 0 0 2 0 0 0 0 4 1
SpindlePole 14 32 71 63 98 103 159 194 102 106 77 144 48 179 157 18 23 28 21 20 19 26 138 125
Vac/Vac Membrane 0 3 0 1 3 5 4 7 2 3 7 6 2 1 4 6 6 3 2 5 9 16 18 21
Unique Cell Count 80 134 160 152 204 252 321 324 324 329 303 328 252 480 573 134 121 133 95 151 192 210 518 459
Labelled Cell Count 132 204 213 240 281 371 469 470 511 485 467 483 365 742 838 174 159 181 95 151 192 210 518 459


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 4.8 5.3 5.2 5.7 4.3 4.3 4.6 3.5 3.6 3.4 3.8 5.5 5.4 5.3 9.0 10.1 9.3 5.1 5.1 5.4
Std Deviation (1e-4) 0.6 0.7 1.0 1.3 1.4 1.2 0.8 1.2 0.7 0.7 0.8 0.7 1.2 1.1 1.2 2.5 2.5 2.3 1.1 1.2 1.4
Intensity Change (Log2) -0.02 0.09 -0.32 -0.29 -0.2 -0.59 -0.58 -0.64 -0.47 0.05 0.02 0.01 0.77 0.94 0.82 -0.06 -0.05 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dhr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available