Standard name
Human Ortholog
Description Protein that promotes the transport and maturation of Pma1 with Exp1; cleaved specifically by Kex2p; localizes to the mid-Golgi and COPI vesicles

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.11 0.08 0.2 0.2 0.31 0.32 0.34 0.35 0.4 0.47 0.5 0.11 0.15 0.25 0.09 0.08 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.32 0.33 0.23 0.1 0.08 0.12 0.06 0.06 0.05 0 0 0
Mitochondria 0.1 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.08 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.93 0.86 0.89 0.89 0.87 0.76 0.75 0.73 0.72 0.64 0.56 0.58 0.73 0.73 0.67 0.84 0.87 0.88 0.84 0.85 0.85 0.9 0.89 0.9
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 2 1 0 0 1 0 4 9 4 1 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 2 0 0 0 0 1 0 0 0 0 0 1 0 2 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 25 25 39 49 92 128 153 107 151 139 132 20 61 85 23 23 18 0 1 0 0 1 2
Endoplasmic Reticulum 0 3 1 0 3 7 5 4 4 6 8 8 4 7 5 10 12 11 1 0 3 0 0 0
Endosome 0 0 1 2 5 7 3 6 3 2 3 2 10 25 25 1 0 3 7 2 11 3 7 4
Golgi 1 9 13 2 3 0 2 2 2 2 3 2 61 131 78 24 24 31 14 13 14 3 4 4
Mitochondria 23 7 5 5 11 9 16 20 8 18 20 18 3 2 6 5 2 6 1 2 5 1 1 2
Nucleus 9 13 11 3 0 4 10 10 8 6 4 2 0 0 1 21 25 16 5 6 2 5 7 4
Nuclear Periphery 0 0 0 1 4 2 2 3 1 0 0 3 0 1 3 1 0 0 4 2 5 2 2 6
Nucleolus 0 1 3 2 1 3 3 5 1 3 4 2 1 1 6 4 1 1 0 0 0 0 3 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 2 1 1 1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 206 191 278 175 218 226 297 328 219 243 166 153 138 292 230 208 260 226 200 195 266 203 259 257
Unique Cell Count 222 223 313 196 250 299 394 451 304 380 297 263 190 398 342 247 298 257 238 230 314 226 290 286
Labelled Cell Count 245 249 337 229 295 351 468 532 356 431 348 325 242 532 444 298 347 312 238 230 314 226 290 286


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.2 23.5 17.6 17.0 17.3 15.6 14.8 14.0 14.0 12.7 12.1 11.5 16.8 14.8 12.3 26.6 27.0 27.3 19.4 18.5 19.1
Std Deviation (1e-4) 4.6 8.2 5.2 4.7 4.8 5.1 5.3 5.0 4.6 4.2 3.9 3.5 4.7 3.1 2.5 10.8 9.6 9.9 6.6 6.1 5.7
Intensity Change (Log2) -0.04 -0.02 -0.17 -0.25 -0.32 -0.33 -0.46 -0.53 -0.62 -0.06 -0.24 -0.52 0.6 0.62 0.64 0.15 0.08 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.9 4.1 7.2 7.9 8.3 8.2 9.6 10.8 11.3 1.0 3.0 5.8 0.6 -0.1 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.2 3.3
Endosome 0 0 0 0 0 0 0 0 0 0 4.2 4.6 0 0 0
Golgi -2.0 -2.1 -3.6 -3.3 -3.6 -2.8 -3.3 -2.4 -2.5 8.6 9.5 6.9 2.6 2.0 3.5
Mitochondria 0 2.0 1.2 1.9 2.2 0.9 2.3 3.2 3.2 0 0 0.2 0 0 0
Nucleus -1.3 0 -1.7 -0.8 -1.1 -0.6 -1.6 -1.7 -2.2 0 0 -3.1 2.5 2.6 1.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.2 -0.6 -4.3 -4.6 -5.4 -5.3 -7.5 -9.1 -8.4 -4.7 -5.1 -6.6 -1.6 -0.6 -0.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.7281 16.1482 12.6162 11.3594 11.7944 14.5357 10.9094 17.2661 14.6668 12.8855 12.7875 15.2559 11.5579 18.7356 14.541 14.7655 14.4139 15.0493
Actin 0.005 0.0001 0.0017 0.0002 0.0041 0.0001 0.0355 0.0007 0.015 0.004 0 0.0009 0.0017 0 0.0006 0.0364 0.0058 0
Bud 0.0006 0.0004 0.0003 0.0001 0.0008 0.0003 0.0015 0.0001 0.0003 0.0006 0.0001 0.0001 0.0002 0.0001 0.0004 0.0001 0.0003 0.0001
Bud Neck 0.0004 0.0003 0.0004 0.0002 0.0004 0.0002 0.0039 0.0002 0.0063 0.0002 0.0002 0.0002 0.0002 0.0001 0.0005 0.0005 0.0002 0.0001
Bud Periphery 0.0011 0.0006 0.0005 0.0003 0.0015 0.0009 0.0033 0.0002 0.0006 0.0009 0.0002 0.0004 0.0004 0.0002 0.0007 0.0002 0.0006 0.0004
Bud Site 0.0009 0.0006 0.0005 0.0002 0.0029 0.0003 0.0076 0.0002 0.0007 0.0024 0.0001 0.0001 0.0002 0 0.0028 0.0002 0.0002 0.0001
Cell Periphery 0.0011 0.0012 0.0015 0.0016 0.001 0.0013 0.0009 0.002 0.0013 0.0019 0.0005 0.0008 0.0014 0.002 0.0014 0.0008 0.0018 0.0009
Cytoplasm 0.0041 0.0065 0.003 0.0011 0.001 0.0007 0.0099 0.0076 0.0013 0.001 0.0005 0.0006 0.0007 0.0004 0.0007 0.0004 0.0007 0.0004
Cytoplasmic Foci 0.0235 0.0018 0.0105 0.004 0.0059 0.0026 0.0176 0.002 0.0018 0.0197 0.0033 0.0014 0.0055 0.0003 0.0025 0.0008 0.0013 0.0003
Eisosomes 0.0001 0 0 0 0.0001 0 0.0002 0 0.0001 0.0001 0 0 0.0001 0 0 0.0008 0.0001 0
Endoplasmic Reticulum 0.0026 0.0028 0.0053 0.0018 0.0013 0.0018 0.0044 0.0014 0.0015 0.0007 0.001 0.0014 0.0014 0.0009 0.0013 0.0009 0.0012 0.0011
Endosome 0.191 0.1247 0.1702 0.1202 0.1268 0.0805 0.0725 0.0451 0.075 0.0285 0.1445 0.0606 0.0987 0.0322 0.076 0.0667 0.042 0.0432
Golgi 0.0377 0.0188 0.0567 0.0117 0.0166 0.0124 0.0122 0.0027 0.0157 0.0037 0.0122 0.0098 0.0172 0.0024 0.0183 0.0048 0.0077 0.0026
Lipid Particles 0.0206 0.0025 0.0108 0.0086 0.0078 0.0126 0.0135 0.0022 0.003 0.0069 0.016 0.0047 0.006 0.0006 0.0072 0.0013 0.0084 0.0008
Mitochondria 0.0311 0.0042 0.0675 0.0212 0.0667 0.0044 0.0179 0.0041 0.0322 0.02 0.0079 0.0231 0.0163 0.0052 0.015 0.001 0.0177 0.0015
None 0.0018 0 0.0004 0.0002 0.0002 0 0.0021 0 0.0001 0.0016 0 0.0001 0.0008 0 0.0001 0.0001 0.0011 0
Nuclear Periphery 0.0063 0.0065 0.0073 0.0026 0.0019 0.0057 0.018 0.0041 0.0029 0.0013 0.0035 0.0145 0.0071 0.002 0.0054 0.0019 0.004 0.0091
Nucleolus 0.0018 0.0004 0.0008 0.0008 0.0004 0.0003 0.0044 0.0004 0.0008 0.0011 0.0004 0.0013 0.0008 0.0002 0.0017 0.0002 0.0005 0.0002
Nucleus 0.0019 0.0012 0.0015 0.0006 0.0007 0.0008 0.015 0.0009 0.0012 0.0012 0.0007 0.0045 0.0012 0.0005 0.0012 0.0005 0.0009 0.0009
Peroxisomes 0.0058 0.0001 0.0028 0.0023 0.003 0.0014 0.006 0 0.0007 0.0045 0.0004 0.0004 0.0019 0 0.0014 0.0006 0.0015 0
Punctate Nuclear 0.0013 0 0.0003 0.0001 0.0001 0 0.0082 0 0.0001 0.0004 0 0.0001 0.0008 0 0.0002 0 0.0007 0
Vacuole 0.2894 0.3859 0.2955 0.3766 0.3391 0.3555 0.3135 0.4551 0.3687 0.3901 0.3906 0.3639 0.3373 0.4551 0.388 0.4194 0.5138 0.4394
Vacuole Periphery 0.3719 0.4412 0.3624 0.4455 0.4176 0.5184 0.4319 0.4707 0.4707 0.5094 0.4177 0.5112 0.5002 0.4978 0.4746 0.4622 0.3895 0.4987

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 122.1326 70.2762 78.8427 92.0263 83.7254 80.4413 102.3547 99.3418 87.7671 100.3581
Translational Efficiency 1.18 1.7143 1.4616 1.1277 1.0738 1.8307 1.2727 1.168 1.2982 1.1505

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
822 235 210 751 2533 1196 132 1246 3355 1431 342 1997

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1452.44 1771.35 1727.86 2430.92 1525.86 1588.87 1851.85 2335.50 1507.87 1618.84 1775.72 2371.38
Standard Deviation 378.19 476.27 229.18 522.47 339.50 343.67 301.20 571.18 350.80 374.88 266.29 555.29
Intensity Change Log 2 0.286371 0.250508 0.743024 0.058378 0.279345 0.614109 0.174067 0.265354 0.678417

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000181 0.000180 0.002053 0.000652 0.000062 0.000154 0.000679 0.001189 0.000091 0.000158 0.001523 0.000987
Bud Neck 0.000836 0.001243 0.002920 0.000656 0.000522 0.000715 0.003677 0.000476 0.000599 0.000802 0.003212 0.000544
Bud Site 0.002326 0.002632 0.002569 0.001999 0.001182 0.001315 0.003328 0.001706 0.001462 0.001531 0.002862 0.001816
Cell Periphery 0.014658 0.009538 0.001669 0.002581 0.022439 0.007604 0.000996 0.002886 0.020532 0.007922 0.001409 0.002771
Cytoplasm 0.000590 0.000384 0.000121 0.000246 0.000106 0.000039 0.000182 0.000155 0.000225 0.000095 0.000144 0.000189
Cytoplasmic Foci 0.002276 0.006551 0.018384 0.001446 0.000361 0.002094 0.008820 0.001305 0.000830 0.002826 0.014693 0.001358
Eisosomes 0.000078 0.000290 0.000064 0.000051 0.000225 0.000167 0.000037 0.000077 0.000189 0.000187 0.000054 0.000067
Endoplasmic Reticulum 0.003326 0.003743 0.002341 0.000908 0.005843 0.003188 0.001009 0.001193 0.005226 0.003279 0.001827 0.001086
Endosome 0.009305 0.016766 0.062746 0.004696 0.004460 0.009184 0.033167 0.004724 0.005647 0.010429 0.051329 0.004714
Golgi 0.011909 0.045663 0.269247* 0.118924* 0.004002 0.011201 0.088620 0.113532* 0.005939 0.016860 0.199531* 0.115560*
Lipid Particles 0.001349 0.002523 0.012215 0.008899 0.002287 0.003251 0.001751 0.008045 0.002057 0.003132 0.008177 0.008366
Mitochondria 0.005985 0.009374 0.004558 0.007500 0.002838 0.010836 0.009969 0.007534 0.003609 0.010596 0.006647 0.007522
Mitotic Spindle 0.000030 0.000241 0.001408 0.000259 0.000012 0.000047 0.003843 0.000126 0.000016 0.000079 0.002348 0.000176
None 0.000232 0.000315 0.000085 0.000474 0.000106 0.000195 0.000097 0.000442 0.000137 0.000215 0.000090 0.000454
Nuclear Periphery 0.000208 0.000296 0.004237 0.001183 0.000342 0.000664 0.003294 0.000875 0.000309 0.000604 0.003873 0.000991
Nuclear Periphery Foci 0.000622 0.000815 0.008506 0.001658 0.000388 0.001309 0.005072 0.001161 0.000445 0.001228 0.007181 0.001348
Nucleolus 0.001862 0.000584 0.000126 0.000299 0.000834 0.000699 0.001011 0.000333 0.001086 0.000680 0.000467 0.000320
Nucleus 0.001959 0.000237 0.000051 0.000103 0.000207 0.000173 0.001191 0.000058 0.000636 0.000184 0.000491 0.000075
Peroxisomes 0.000159 0.000418 0.000459 0.000067 0.000039 0.000086 0.000454 0.000033 0.000068 0.000140 0.000457 0.000046
Vacuole 0.701226* 0.719107* 0.390571* 0.269840* 0.792632* 0.766153* 0.559551* 0.285603* 0.770237* 0.758427* 0.455792* 0.279675*
Vacuole Periphery 0.240881* 0.179102* 0.215668* 0.577558* 0.161115* 0.180926* 0.273251* 0.568548* 0.180658* 0.180627* 0.237893* 0.571937*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.12 -3.23 -2.44 -2.52 2.31 -3.18 -3.04 -2.54 -2.33 -1.04 -2.41 -3.94 -3.13 -2.90 1.14
Bud Neck -1.16 -4.15 0.64 1.49 4.27 -2.42 -2.87 0.62 2.80 2.90 -2.23 -5.03 0.44 2.11 5.06
Bud Site -0.34 -0.34 0.19 0.38 0.37 -0.28 -2.18 -0.97 -0.82 1.58 -0.15 -2.64 -0.67 -0.59 1.46
Cell Periphery 1.98 6.41 5.92 3.75 -0.98 9.26 16.05 14.21 4.59 -3.64 9.20 16.41 15.38 5.68 -2.48
Cytoplasm 0.74 1.57 -0.32 -0.66 -0.97 1.20 -0.96 -0.75 -2.61 0.35 1.49 1.02 -0.64 -1.10 -0.96
Cytoplasmic Foci -1.77 -4.75 1.25 2.17 5.07 -3.21 -3.25 -4.17 1.35 2.88 -3.26 -6.04 -2.33 2.38 5.80
Eisosomes -2.07 0.67 1.90 2.35 0.80 1.53 8.71 5.73 2.37 -1.90 0.06 7.56 6.62 3.48 -0.85
Endoplasmic Reticulum -0.26 1.70 7.54 1.82 2.70 7.18 7.59 13.13 6.04 -0.25 4.95 7.67 15.51 6.10 1.84
Endosome -2.06 -8.29 2.72 3.41 9.08 -3.43 -4.36 -0.36 3.13 4.31 -3.66 -9.69 1.25 4.30 9.88
Golgi -3.45 -12.19 -13.33 -5.96 6.72 -3.88 -5.77 -18.10 -16.41 -1.58 -4.80 -13.02 -22.82 -18.99 5.39
Lipid Particles -1.55 -3.08 -6.73 -4.79 0.89 -2.40 1.43 -5.02 -3.99 -5.27 -3.02 -2.80 -7.59 -5.88 -0.09
Mitochondria -0.93 0.95 -0.86 0.52 -2.18 -5.42 -2.64 -4.19 1.92 0.85 -5.06 -2.47 -4.29 2.05 -0.66
Mitotic Spindle -1.21 -2.45 -2.03 -0.16 1.96 -2.08 -1.74 -5.54 -3.02 1.69 -1.94 -2.52 -3.51 -1.83 2.34
None -0.63 1.82 -2.79 -1.54 -11.66 -3.19 0.49 -11.27 -6.80 -11.91 -2.24 2.03 -10.50 -7.21 -16.64
Nuclear Periphery -1.18 -3.10 -6.64 -5.47 2.34 -3.09 -2.35 -4.88 -1.50 1.92 -3.37 -3.82 -7.67 -3.29 3.08
Nuclear Periphery Foci -0.97 -3.39 -3.32 -2.57 2.92 -2.64 -3.06 -8.73 0.42 2.56 -2.65 -4.37 -7.12 -0.39 3.77
Nucleolus 1.75 2.37 2.14 4.03 -4.06 2.27 -0.46 9.99 7.83 1.69 2.19 2.53 4.16 9.15 0.94
Nucleus 1.81 2.00 1.77 -0.14 -1.34 1.28 -0.98 9.60 4.97 1.11 1.92 0.28 2.06 0.75 0.91
Peroxisomes -1.23 -2.46 1.83 1.70 3.37 -3.63 -3.94 0.65 3.46 3.99 -1.93 -4.79 1.46 2.55 5.06
Vacuole -0.90 14.86 35.82* 23.22 5.95 3.31 10.82 70.09* 53.46* 12.44 1.57 20.55 79.89* 60.32* 11.34
Vacuole Periphery 3.96 1.54 -26.94* -24.99* -21.51* -2.83 -6.56 -50.26* -42.44* -16.36* 0.00 -4.97 -58.39 -50.88* -27.31*