ORF
Human Ortholog
Description NADH-dependent aldehyde reductase; involved in detoxification of furfural; expression is upregulated in cells treated with the aldehydes furfural and glycolaldehyde, the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0.06 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.98 1.0 0.98 0.97 0.94 0.88 0.8 0.75 0.74 0.74 0.82 – 1.0 0.96 1.0 0.98 0.98 0.96 0.94 0.97 0.86 0.94 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0.13 0.15 0.2 0.18 0.2 0.12 – 0 0 0 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 4 10 2 3 – 0 1 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 1 1 1 1 0 – 0 2 0 0 0 0 0 0 0 0 8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 2 0 5 9 6 17 11 14 8 9 – 1 0 1 2 0 0 0 0 0 0 0
Cytoplasm 34 42 226 180 247 199 272 253 188 192 131 124 – 101 72 26 128 62 48 62 212 23 72 103
Endoplasmic Reticulum 0 0 2 2 4 8 39 49 49 47 35 19 – 0 1 0 6 4 0 0 0 0 1 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 1 1 0 1 3 8 8 5 2 – 0 0 0 0 1 0 0 1 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 2 0 0 0 – 0 0 0 1 0 0 0 0 1 0 0
Nucleus 0 1 0 0 0 0 0 0 1 1 0 0 – 0 0 0 0 0 0 1 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 0 1 0 1 0 1 1 1 – 0 0 0 0 0 0 0 0 0 0 2
Unique Cell Count 34 43 227 183 254 211 310 318 251 259 176 152 101 75 26 130 63 50 66 218 27 77 119
Labelled Cell Count 34 43 231 183 257 218 318 324 264 276 183 158 102 77 27 137 68 50 66 218 27 77 119


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 183.6 188.8 185.5 171.5 148.1 108.3 90.7 79.1 73.2 70.3 68.4 67.9 – 179.8 165.5 181.0 214.7 241.6 – 178.9 166.8
Std Deviation (1e-4) 39.4 57.5 42.4 50.7 31.5 25.5 21.5 18.7 18.1 17.0 17.1 15.8 – 51.1 51.4 45.4 66.2 62.1 – 77.3 91.1
Intensity Change (Log2) – – – -0.11 -0.33 -0.78 -1.03 -1.23 -1.34 -1.4 -1.44 -1.45 – -0.04 -0.17 -0.04 0.21 0.38 – -0.05 -0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 2.3 0 2.8 2.4 2.8 0 0 – 0 0 0 0 0
Cytoplasm -1.2 -2.0 -3.2 -5.2 -7.1 -7.9 -8.1 -7.9 -6.5 – 0.7 -2.3 0.3 -1.1 -1.0
Endoplasmic Reticulum 0 0 0 5.0 5.7 6.6 6.3 6.6 4.8 – 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 201.4193 245.4846 228.0018 234.4746 225.8997 135.6324 185.2001 237.7955 223.9758 210.3834 159.413 222.6508 217.5909 275.8109 253.2596 232.7291 235.6295 234.6975
Actin 0.0013 0.0005 0.003 0.0004 0.0017 0.0005 0.0021 0.0027 0.0008 0.0005 0.004 0.0009 0.0198 0.0011 0.0106 0.0006 0.0868 0.0213
Bud 0.0015 0.0017 0.0038 0.0031 0.0019 0.0076 0.0053 0.0021 0.0039 0.0043 0.0109 0.0172 0.0055 0.0041 0.0072 0.0045 0.0036 0.0138
Bud Neck 0.0009 0.0013 0.0013 0.0019 0.0014 0.0007 0.0009 0.001 0.0011 0.0009 0.0015 0.0013 0.0008 0.0008 0.0034 0.0012 0.0011 0.0035
Bud Periphery 0.0025 0.0015 0.0048 0.0023 0.0027 0.0113 0.0051 0.001 0.0027 0.0023 0.009 0.0098 0.0058 0.002 0.0042 0.0056 0.0011 0.0068
Bud Site 0.0009 0.001 0.0016 0.0004 0.0005 0.001 0.0016 0.0019 0.0014 0.0009 0.0026 0.001 0.002 0.0034 0.0065 0.0008 0.0037 0.0042
Cell Periphery 0.0812 0.0443 0.0384 0.0096 0.0266 0.0537 0.0745 0.0109 0.0382 0.0494 0.0124 0.01 0.0552 0.022 0.0311 0.02 0.0015 0.0064
Cytoplasm 0.4328 0.7109 0.592 0.4772 0.5328 0.0748 0.5233 0.8527 0.6762 0.4304 0.4578 0.4692 0.5489 0.849 0.6935 0.3796 0.6664 0.5824
Cytoplasmic Foci 0.0061 0.001 0.0034 0.002 0.0095 0.0021 0.0062 0.0012 0.0018 0.0014 0.0931 0.0022 0.0035 0.0011 0.0125 0.001 0.0085 0.0072
Eisosomes 0.001 0.0046 0.0003 0 0.0002 0.0002 0.0009 0.0049 0.0003 0 0.0003 0 0.0009 0.0001 0.0008 0 0.0005 0.0009
Endoplasmic Reticulum 0.0539 0.0241 0.0142 0.0419 0.0311 0.2609 0.0222 0.0032 0.0054 0.001 0.0054 0.0023 0.0132 0.0014 0.0042 0.0172 0.0004 0.0297
Endosome 0.0092 0.0034 0.011 0.0124 0.0228 0.0073 0.0068 0.002 0.01 0.0036 0.0121 0.0141 0.0042 0.0011 0.003 0.0041 0.0048 0.0112
Golgi 0.0028 0.0007 0.0018 0.0005 0.0024 0.0015 0.0014 0.0003 0.0019 0.0002 0.0012 0.0009 0.0026 0.0002 0.0015 0.0003 0.0027 0.0032
Lipid Particles 0.0038 0.0017 0.0021 0.0007 0.0034 0.002 0.0021 0.0005 0.0011 0.0012 0.0252 0.0002 0.0023 0.0004 0.0046 0.0006 0.0083 0.0017
Mitochondria 0.0173 0.0047 0.0082 0.001 0.0082 0.1209 0.028 0.0106 0.0039 0.0015 0.0454 0.0022 0.0239 0.0045 0.0113 0.0039 0.004 0.0666
None 0.0044 0.0003 0.0045 0.0002 0.0008 0.0031 0.0106 0.0007 0.001 0.0004 0.0147 0.0003 0.0056 0.001 0.0124 0.0009 0.0008 0.0066
Nuclear Periphery 0.0327 0.0059 0.0125 0.0158 0.0222 0.0152 0.0294 0.004 0.0108 0.0084 0.0096 0.0028 0.0423 0.0057 0.0131 0.0234 0.0062 0.0315
Nucleolus 0.0063 0.0006 0.0018 0.0006 0.003 0.0156 0.0076 0.004 0.0025 0.004 0.0061 0.0007 0.0174 0.0031 0.0058 0.0039 0.0008 0.009
Nucleus 0.0101 0.0039 0.0071 0.007 0.0059 0.031 0.0182 0.0071 0.0095 0.0143 0.0116 0.0072 0.0288 0.0108 0.0162 0.026 0.0133 0.0258
Peroxisomes 0.003 0.0001 0.0004 0.0001 0.023 0.0013 0.0005 0.0002 0.0001 0.0002 0.0028 0.0003 0.0007 0.0003 0.0036 0.0002 0.0547 0.0019
Punctate Nuclear 0.0008 0 0.0014 0 0.0002 0.0001 0.0003 0.0001 0.0001 0 0.0053 0 0.0005 0 0.0024 0.0001 0.0007 0.0007
Vacuole 0.2511 0.1642 0.2256 0.335 0.1803 0.2131 0.1875 0.0753 0.1839 0.3669 0.1912 0.3562 0.142 0.0724 0.1186 0.301 0.0866 0.1085
Vacuole Periphery 0.0766 0.0236 0.0609 0.0876 0.1192 0.1763 0.0655 0.0135 0.0436 0.1082 0.0776 0.1014 0.074 0.0156 0.0335 0.2049 0.0436 0.0572

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✔
Gene Expression –
Translational Efficiency –