Standard name
Description PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.06 0.05 0.07 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.96 0.96 0.99 0.95 0.83 0.88 0.84 0.76 0.75 0.74 1.0 1.0 0.99 0.93 0.92 0.96 0.95 0.91 0.78 0.92 0.93 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.15 0.08 0.2 0.4 0.27 0.49 0.44 0.51 0.52 0 0 0 0 0.05 0 0 0 0.1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.07 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2 0 0 1 2
Bud 0 3 4 0 0 5 1 2 0 4 2 3 1 1 3 0 0 0 0 4 0 1 1 1
Bud Neck 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1
Bud Site 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0
Cell Periphery 16 1 1 0 1 4 0 3 3 3 3 2 3 5 11 10 4 9 0 0 0 0 0 0
Cytoplasm 546 407 131 90 115 153 170 231 90 92 38 81 224 274 285 165 73 116 537 387 118 192 277 248
Endoplasmic Reticulum 6 2 1 1 0 0 0 2 0 0 0 0 0 0 0 4 6 5 0 1 2 1 4 1
Endosome 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 6 4 1 0 1 5
Golgi 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 2 1
Mitochondria 3 12 7 14 9 33 83 72 52 53 26 57 0 0 2 6 4 2 7 7 14 2 4 1
Nucleus 0 0 0 0 0 1 0 1 1 1 1 2 1 0 3 6 2 0 0 1 0 0 0 0
Nuclear Periphery 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 1 0
Nucleolus 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 1 0 0 1 0 0 0 0 0 1 0 0 1 0 0 0 0
Vac/Vac Membrane 1 1 0 0 0 0 1 0 0 1 2 1 0 0 0 10 2 5 0 4 10 8 1 3
Unique Cell Count 555 420 137 94 116 161 206 263 107 121 51 110 225 274 287 177 79 121 563 425 153 209 298 271
Labelled Cell Count 578 429 146 106 125 196 255 313 146 154 73 146 230 280 306 202 93 137 563 425 153 209 298 271


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 4.5 3.9 3.9 4.0 3.9 3.7 3.6 3.5 3.4 3.5 3.8 5.4 5.4 5.1 7.9 7.6 8.2 5.4 5.7 6.2
Std Deviation (1e-4) 0.6 0.7 0.8 1.1 0.6 1.1 1.3 1.0 1.7 1.3 0.9 1.7 1.3 0.9 1.0 2.1 2.0 2.4 1.1 1.4 1.8
Intensity Change (Log2) 0.01 0.05 0.01 -0.08 -0.12 -0.16 -0.2 -0.17 -0.05 0.47 0.48 0.39 1.01 0.95 1.07 0.46 0.56 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Psk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available