ORF
Description Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.97 0.93 0.88 0.89 0.75 0.67 0.73 0.66 0.64 0.99 0.99 0.99 0.75 0.78 0.83 0.95 0.91 0.92 0.96 0.85 0.77
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0.06 0 0 0 0 0.06 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0 0.12 0.10 0.14 0.17 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0.08 0.16 0.07 0.24 0.23 0.21 0.23 0.19 0 0 0 0.29 0.27 0.22 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 2 0 3 0 0 0 0 1 0 1 0 2 0 4 2
Bud 0 0 1 0 0 2 1 3 1 1 5 0 0 0 0 0 0 1 1 0 0 4 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 0 2 2 4 0 11 3 11 5 13 0 0 1 2 2 4 0 0 0 0 0 0
Cytoplasm 508 164 287 257 288 332 433 306 324 406 331 480 523 710 112 120 90 497 152 274 335 531 482
Endoplasmic Reticulum 3 1 4 0 0 1 1 5 5 4 13 3 4 1 6 1 4 2 0 1 1 7 7
Endosome 0 0 0 0 1 1 1 0 1 1 1 0 0 0 14 7 6 1 0 2 1 36 50
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 1 4 1 0 2 0 0 1 12 22
Mitochondria 1 0 0 1 0 33 12 53 46 86 91 1 0 0 4 1 2 1 1 3 0 1 3
Nucleus 3 0 2 6 3 8 15 23 8 28 25 6 2 2 5 0 1 2 6 3 3 2 3
Nuclear Periphery 1 0 0 0 0 0 0 1 0 2 1 0 0 0 0 0 0 1 0 0 0 2 3
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 3 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 1 1 0 1 1 0 0 0 2 5 1 1 0 1 0 7 18
Vac/Vac Membrane 14 0 16 22 53 25 138 103 92 143 100 2 10 16 43 41 24 2 1 2 3 5 11
Unique Cell Count 520 165 297 276 327 374 575 457 441 618 520 485 530 719 149 153 109 521 168 298 349 622 626
Labelled Cell Count 532 165 313 288 349 402 613 498 490 677 584 492 539 731 196 182 132 521 168 298 349 622 626

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm, Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.0 10.1 9.6 9.1 10.1 6.9 8.9 7.3 8.3 7.7 7.8 10.7 10.0 10.1 11.6 11.7 11.8 9.1 9.3 9.3
Std Deviation (1e-4) 1.5 1.9 1.4 1.4 1.5 1.5 1.5 1.8 1.5 1.4 1.4 1.8 1.6 1.4 1.8 2.1 1.7 1.3 1.5 1.6
Intensity Change (Log2) -0.07 0.08 -0.47 -0.12 -0.4 -0.21 -0.32 -0.3 0.15 0.06 0.07 0.27 0.28 0.3 -0.07 -0.05 -0.05

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 1.8 0 1.9 0 0 0 0 0 0
Cytoplasm 1.9 -1.1 -4.2 -2.7 -6.9 -4.0 -7.9 -5.7 3.9 -0.2 -7.4 -4.1 -4.4 -2.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 1.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.2 0 6.1 5.7 6.8 7.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.5 3.7 1.7 4.6 5.2 2.2 4.1 2.3 0 -2.8 0 1.4 3.7 1.9