ORF
Human Ortholog
Description Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.97 0.93 0.88 0.89 0.75 0.67 0.73 0.66 0.64 0.99 0.99 0.99 0.75 0.78 0.83 0.95 0.91 0.92 0.96 0.85 0.77
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0.06 0 0 0 0 0.06 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0 0.12 0.1 0.14 0.17 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0.08 0.16 0.07 0.24 0.23 0.21 0.23 0.19 0 0 0 0.29 0.27 0.22 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 2 0 3 0 0 0 0 1 0 1 0 2 0 4 2
Bud 0 0 1 0 0 2 1 3 1 1 5 0 0 0 0 0 0 1 1 0 0 4 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 0 2 2 4 0 11 3 11 5 13 0 0 1 2 2 4 0 0 0 0 0 0
Cytoplasm 508 164 287 257 288 332 433 306 324 406 331 480 523 710 112 120 90 497 152 274 335 531 482
Endoplasmic Reticulum 3 1 4 0 0 1 1 5 5 4 13 3 4 1 6 1 4 2 0 1 1 7 7
Endosome 0 0 0 0 1 1 1 0 1 1 1 0 0 0 14 7 6 1 0 2 1 36 50
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 1 4 1 0 2 0 0 1 12 22
Mitochondria 1 0 0 1 0 33 12 53 46 86 91 1 0 0 4 1 2 1 1 3 0 1 3
Nucleus 3 0 2 6 3 8 15 23 8 28 25 6 2 2 5 0 1 2 6 3 3 2 3
Nuclear Periphery 1 0 0 0 0 0 0 1 0 2 1 0 0 0 0 0 0 1 0 0 0 2 3
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 3 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 1 1 0 1 1 0 0 0 2 5 1 1 0 1 0 7 18
Vac/Vac Membrane 14 0 16 22 53 25 138 103 92 143 100 2 10 16 43 41 24 2 1 2 3 5 11
Unique Cell Count 520 165 297 276 327 374 575 457 441 618 520 485 530 719 149 153 109 521 168 298 349 622 626
Labelled Cell Count 532 165 313 288 349 402 613 498 490 677 584 492 539 731 196 182 132 521 168 298 349 622 626


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.0 10.1 9.6 9.1 10.1 6.9 8.9 7.3 8.3 7.7 7.8 10.7 10.0 10.1 11.6 11.7 11.8 9.1 9.3 9.3
Std Deviation (1e-4) 1.5 1.9 1.4 1.4 1.5 1.5 1.5 1.8 1.5 1.4 1.4 1.8 1.6 1.4 1.8 2.1 1.7 1.3 1.5 1.6
Intensity Change (Log2) -0.07 0.08 -0.47 -0.12 -0.4 -0.21 -0.32 -0.3 0.15 0.06 0.07 0.27 0.28 0.3 -0.07 -0.05 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 1.8 0 1.9 0 0 0 0 0 0
Cytoplasm -1.9 -4.0 -3.8 -7.9 -9.7 -8.2 -10.2 -10.6 2.3 2.0 2.3 -7.0 -6.2 -4.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 1.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.2 0 6.1 5.7 6.8 7.6 0 0 0 0 0 0
Nucleus 0 0 0 2.0 3.3 0 3.1 3.2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.2 4.3 0.7 6.8 6.3 5.8 6.6 5.5 -4.5 -2.8 -2.6 6.9 6.5 5.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.5132 6.5219 5.8415 4.9497 4.8326 5.5686 7.0743 9.5283 9.0055 8.2337 8.0293 8.2483 7.4551 10.181 9.3164 8.7496 9.2251 9.1999
Actin 0.0104 0.0003 0.0031 0.004 0.0128 0.002 0.0053 0.0003 0.0002 0.0137 0.0024 0.0014 0.0602 0.0005 0.0042 0.0825 0.0254 0.0041
Bud 0.0011 0.0004 0.0002 0.0002 0.0004 0.0003 0.0006 0.0002 0.0001 0.0005 0.0003 0.0001 0.0023 0.0001 0.0007 0.0008 0.0006 0.0002
Bud Neck 0.0062 0.0003 0.0008 0.001 0.0025 0.0017 0.0011 0.0004 0.0004 0.0037 0.0016 0.0015 0.0011 0.0002 0.0003 0.0013 0.0011 0.0011
Bud Periphery 0.0013 0.0002 0.0001 0.0001 0.0007 0.0003 0.0003 0 0 0.0006 0.0004 0.0001 0.0025 0 0.0005 0.0008 0.0004 0.0001
Bud Site 0.0038 0.0021 0.0004 0.0018 0.0005 0.0003 0.0006 0.0006 0.0001 0.0005 0.0013 0.0001 0.0021 0.0003 0.0003 0.0093 0.0018 0.0001
Cell Periphery 0.0003 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0 0.0001 0.0001 0 0.0002 0.0001 0 0.0004 0.0001 0.0001
Cytoplasm 0.8526 0.9401 0.8995 0.8872 0.8444 0.8968 0.867 0.9464 0.9308 0.8738 0.8512 0.8839 0.7788 0.9816 0.9227 0.7066 0.8783 0.9187
Cytoplasmic Foci 0.0108 0.002 0.0027 0.0039 0.0076 0.0066 0.008 0.0024 0.0019 0.0027 0.0049 0.0081 0.0428 0.0009 0.013 0.0384 0.014 0.0055
Eisosomes 0.0001 0 0 0 0.0002 0 0.0001 0 0 0 0.0001 0 0.0003 0 0 0.0003 0.0001 0
Endoplasmic Reticulum 0.0051 0.0021 0.0023 0.0065 0.0046 0.0038 0.0053 0.0012 0.0009 0.0022 0.0032 0.0022 0.0082 0.0021 0.0014 0.0042 0.0044 0.0019
Endosome 0.0164 0.0018 0.0036 0.005 0.0283 0.0079 0.0135 0.001 0.0007 0.003 0.0154 0.006 0.0228 0.0002 0.0038 0.0682 0.0236 0.0104
Golgi 0.0033 0.0001 0.0014 0.0005 0.0036 0.0012 0.0019 0.0001 0 0.0006 0.0014 0.0006 0.0076 0 0.0026 0.0184 0.0087 0.0042
Lipid Particles 0.0062 0 0.0052 0.0002 0.0088 0.0005 0.0012 0 0 0.0001 0.0018 0.0002 0.0073 0 0.0002 0.0116 0.0088 0.0085
Mitochondria 0.0091 0.0002 0.0074 0.0007 0.0032 0.0009 0.0021 0.0002 0.0001 0.0013 0.0015 0.0005 0.0105 0.0001 0.0056 0.0042 0.0011 0.0042
None 0.0152 0.0037 0.0024 0.0065 0.0127 0.0032 0.0127 0.0027 0.0037 0.0052 0.0077 0.0052 0.0203 0.0075 0.0157 0.0029 0.0045 0.0023
Nuclear Periphery 0.0123 0.004 0.0039 0.0078 0.021 0.0062 0.0099 0.0026 0.0024 0.005 0.0171 0.0055 0.0053 0.0005 0.0015 0.0022 0.0046 0.0043
Nucleolus 0.0004 0.0001 0.0001 0.0002 0.006 0.0001 0.0002 0 0 0.0001 0.0033 0.0001 0.003 0 0 0.0003 0.0002 0.0007
Nucleus 0.0338 0.0403 0.0619 0.0701 0.0248 0.0552 0.0635 0.0397 0.0563 0.0836 0.0639 0.0796 0.0129 0.0057 0.0176 0.0112 0.0141 0.0293
Peroxisomes 0.0048 0 0.0023 0.0002 0.003 0.0048 0.0012 0.0001 0 0.0002 0.0004 0.0006 0.0063 0 0.0084 0.0289 0.002 0.0007
Punctate Nuclear 0.003 0.001 0.0017 0.0029 0.0114 0.0067 0.0031 0.0014 0.0016 0.0017 0.0182 0.0031 0.0033 0.0001 0.0009 0.0023 0.0049 0.0018
Vacuole 0.0028 0.0011 0.0007 0.001 0.0018 0.0012 0.0017 0.0005 0.0003 0.001 0.0029 0.0008 0.0012 0.0002 0.0003 0.0044 0.001 0.0008
Vacuole Periphery 0.0011 0.0002 0.0003 0.0003 0.0017 0.0004 0.0007 0.0001 0.0001 0.0003 0.0009 0.0002 0.0009 0 0.0001 0.0009 0.0004 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.5449 52.6794 16.1753 30.853 28.9811 24.3711 43.1623 48.9659 33.6484 39.2164
Translational Efficiency 1.0713 0.9722 2.1414 0.7894 1.145 1.6927 1.7307 0.9683 1.4466 1.0809

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
256 582 171 993 1634 2161 674 1503 1890 2743 845 2496

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 931.44 1123.34 1311.30 1227.41 936.95 1096.96 1215.45 1199.92 936.20 1102.56 1234.85 1210.86
Standard Deviation 108.57 163.50 154.09 180.35 121.92 145.48 152.30 166.01 120.21 149.87 157.45 172.38
Intensity Change Log 2 0.270260 0.493463 0.398082 0.227467 0.375447 0.356894 0.248959 0.435487 0.377575

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000262 0.000245 0.001318 0.000594 0.000147 0.000181 0.000428 0.000556 0.000163 0.000194 0.000608 0.000571
Bud Neck 0.002230 0.000918 0.003402 0.003893 0.001102 0.002289 0.002080 0.003842 0.001255 0.001998 0.002347 0.003862
Bud Site 0.007243 0.005712 0.007632 0.019356 0.004642 0.005285 0.013773 0.019196 0.004994 0.005376 0.012530 0.019260
Cell Periphery 0.000168 0.000070 0.000379 0.000266 0.000082 0.000047 0.000149 0.000100 0.000094 0.000052 0.000195 0.000166
Cytoplasm 0.642506* 0.824531* 0.398015* 0.522601* 0.782757* 0.805258* 0.621354* 0.583959* 0.763760* 0.809347* 0.576158* 0.559549*
Cytoplasmic Foci 0.182876* 0.045281 0.015634 0.018162 0.062048 0.033603 0.011008 0.023892 0.078414 0.036081 0.011944 0.021612
Eisosomes 0.000056 0.000010 0.000048 0.000015 0.000017 0.000009 0.000017 0.000015 0.000022 0.000009 0.000023 0.000015
Endoplasmic Reticulum 0.001224 0.000622 0.007326 0.001690 0.001121 0.000495 0.002411 0.001087 0.001135 0.000522 0.003406 0.001327
Endosome 0.009164 0.001770 0.005095 0.006019 0.002817 0.002408 0.001400 0.011483 0.003676 0.002273 0.002147 0.009309
Golgi 0.000771 0.000743 0.000355 0.032531 0.000587 0.001249 0.000153 0.010241 0.000612 0.001142 0.000194 0.019109
Lipid Particles 0.001956 0.000459 0.002663 0.001120 0.000829 0.000448 0.000830 0.000842 0.000981 0.000450 0.001201 0.000953
Mitochondria 0.000124 0.000743 0.000461 0.001529 0.000844 0.001160 0.000204 0.005231 0.000747 0.001071 0.000256 0.003758
Mitotic Spindle 0.000693 0.002578 0.012101 0.141660* 0.002786 0.007984 0.009794 0.116283* 0.002503 0.006837 0.010261 0.126379*
None 0.000934 0.004385 0.001900 0.009545 0.003989 0.005690 0.008390 0.009293 0.003575 0.005413 0.007077 0.009393
Nuclear Periphery 0.000235 0.000209 0.001544 0.000910 0.000185 0.000199 0.000624 0.000759 0.000191 0.000201 0.000810 0.000819
Nuclear Periphery Foci 0.003285 0.003507 0.011656 0.004844 0.002662 0.001035 0.005299 0.002930 0.002746 0.001560 0.006586 0.003692
Nucleolus 0.000480 0.000311 0.000197 0.000521 0.000272 0.000249 0.000174 0.000524 0.000300 0.000262 0.000179 0.000523
Nucleus 0.066347 0.074860 0.374703* 0.142907* 0.099657 0.095253 0.250356* 0.127501 0.095145 0.090926 0.275520* 0.133630*
Peroxisomes 0.001419 0.000317 0.000240 0.001135 0.000398 0.000710 0.000210 0.001627 0.000536 0.000626 0.000216 0.001431
Vacuole 0.077644 0.030946 0.155073 0.080121 0.032584 0.035198 0.071253 0.072467 0.038687 0.034295 0.088215 0.075512
Vacuole Periphery 0.000384 0.001784 0.000256 0.010581 0.000475 0.001249 0.000094 0.008172 0.000463 0.001363 0.000127 0.009131

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.19 -8.52 -3.32 -3.20 6.04 -0.86 -9.33 -6.07 -3.99 3.50 -1.14 -12.20 -6.88 -5.16 5.31
Bud Neck 1.84 -4.15 -5.87 -8.42 -1.69 -2.98 -5.38 -10.54 -5.89 -4.77 -2.55 -6.86 -13.13 -8.41 -5.49
Bud Site 0.72 -0.85 -4.38 -5.11 -3.89 -0.51 -2.28 -6.59 -6.44 -2.10 -0.48 -2.46 -8.21 -8.29 -2.80
Cell Periphery 5.07 -6.36 0.63 -4.80 6.88 4.96 -6.89 0.05 -5.26 7.11 6.31 -9.59 -2.27 -7.29 7.65
Cytoplasm -9.71 13.12 14.25 35.33 -3.51 -2.47 26.70 35.43 38.26 2.75 -5.96 29.97 43.89 52.81 2.38
Cytoplasmic Foci 11.82 15.38 14.74 7.42 -2.38 9.96 20.72 15.78 7.87 -5.28 13.70 24.30 20.44 10.57 -5.81
Eisosomes 9.05 -0.14 6.47 -6.83 6.94 6.44 -4.43 -1.72 -10.22 3.47 9.96 -4.65 0.85 -12.98 6.02
Endoplasmic Reticulum 3.51 -6.77 0.38 -4.27 6.88 5.00 -3.27 2.30 -4.20 4.30 5.58 -6.61 1.45 -6.15 7.27
Endosome 6.54 3.21 6.08 -1.47 4.58 1.19 5.05 -1.50 -2.19 -4.12 4.02 4.78 1.24 -2.23 -2.52
Golgi 0.28 2.55 -2.00 -2.04 -2.24 -1.47 4.30 -2.05 -1.03 -2.69 -1.42 4.63 -2.83 -2.10 -3.36
Lipid Particles 4.64 -2.87 -1.63 -12.10 2.04 3.79 -4.42 -6.47 -11.49 -2.15 5.41 -5.98 -8.75 -16.85 -2.28
Mitochondria -1.12 -7.93 -6.14 -0.07 -1.38 -0.62 1.76 -0.93 -0.38 -2.42 -0.72 1.48 -0.89 -0.13 -2.79
Mitotic Spindle -2.48 -1.64 -13.74 -13.41 -11.43 -3.10 -2.26 -17.27 -16.33 -15.72 -3.22 -2.64 -21.86 -20.75 -19.53
None -8.12 -3.38 -12.53 -6.32 -10.72 -2.20 -4.36 -6.79 -3.92 -1.26 -2.92 -4.16 -9.42 -5.76 -3.68
Nuclear Periphery -0.00 -12.19 -18.98 -17.83 -0.64 -0.84 -19.62 -20.82 -19.20 -5.56 -0.87 -22.71 -29.46 -26.88 -6.63
Nuclear Periphery Foci -0.71 -4.80 -4.18 -3.42 2.11 4.02 -4.64 -3.73 -7.52 0.34 3.04 -6.41 -5.59 -9.10 0.79
Nucleolus 0.60 0.95 -1.64 -3.77 -6.66 0.47 0.74 -4.11 -4.37 -4.45 0.65 1.13 -5.14 -5.77 -6.05
Nucleus -2.37 -14.28 -13.46 -13.73 9.21 0.74 -22.33 -11.17 -12.33 14.54 0.34 -26.02 -16.20 -17.88 16.93
Peroxisomes 3.00 3.22 2.04 -1.82 -2.41 -2.22 1.33 -1.68 0.20 -2.59 -0.89 2.52 -0.95 -0.11 -3.24
Vacuole 4.75 -7.96 -15.24 -22.13 -0.18 -2.84 -15.08 -23.62 -22.21 -2.43 -0.65 -17.32 -31.79 -31.65 -3.64
Vacuole Periphery -0.94 2.01 -1.43 0.07 -1.58 -1.45 3.53 -1.73 -0.45 -2.38 -1.71 3.56 -2.18 -0.36 -2.92