Standard name
Human Ortholog
Description FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier and is involved in gene tethering at the nuclear periphery; NUP100 has a paralog, NUP116, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.12 0.06 0.09 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.24 0.14 0.11 0.08 0.33 0.23 0.46 0.37 0.41 0.39 0 0 0 0.05 0.07 0.07 0 0 0 0.07 0.13 0.17
Nucleus 0 0.08 0 0.06 0.05 0 0 0 0.05 0.05 0 0.07 0.07 0.08 0 0.07 0.05 0 0 0 0 0 0
Nuclear Periphery 0.88 0.83 0.82 0.82 0.76 0.73 0.61 0.65 0.63 0.63 0.64 0.81 0.81 0.7 0.72 0.64 0.64 0.91 0.86 0.87 0.87 0.74 0.67
Nucleolus 0.12 0.07 0.13 0.16 0.21 0.26 0.26 0.26 0.26 0.27 0.35 0.05 0.05 0 0.1 0.11 0.19 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.29 0.27 0.18 0.1 0.15 0.09 0.11 0.05 0.05 0.05 0.05 0.23 0.23 0.26 0.25 0.33 0.26 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 4 5
Bud 0 0 0 0 0 1 0 3 2 1 4 1 1 0 1 0 0 2 1 1 1 0 3
Bud Neck 0 0 0 0 0 0 0 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 3 3 5 5 2 0 0 2 0 0 1
Cell Periphery 1 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 2 3 6 12 11 10 18 10 8 10 3 14 14 20 6 9 13 0 0 0 1 1 0
Endoplasmic Reticulum 7 0 0 1 0 1 1 1 2 2 1 1 0 2 5 1 0 0 0 0 1 0 1
Endosome 1 0 9 4 18 23 43 16 16 11 13 2 1 1 3 4 3 1 2 3 2 5 20
Golgi 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 2 2 0 2 0 4 11
Mitochondria 9 28 42 31 31 179 187 257 252 194 269 2 2 2 5 7 8 2 2 10 18 38 72
Nucleus 5 9 8 16 18 20 34 13 34 24 24 13 13 13 4 7 6 1 0 3 0 2 2
Nuclear Periphery 192 96 253 233 293 392 503 365 423 297 440 158 158 119 76 67 75 212 105 296 218 213 290
Nucleolus 26 8 41 46 83 138 214 146 176 125 238 9 9 4 10 11 22 1 1 4 2 2 2
Peroxisomes 0 0 1 0 1 5 6 2 3 0 3 0 0 0 0 0 0 0 0 1 1 4 7
SpindlePole 0 0 0 0 1 3 7 1 6 3 4 5 5 6 3 0 2 0 0 1 0 1 2
Vac/Vac Membrane 63 31 55 29 59 50 92 28 33 23 34 46 45 44 26 34 30 6 4 8 1 10 14
Unique Cell Count 218 116 310 284 388 538 829 560 674 471 683 196 196 171 105 104 117 232 122 340 251 290 435
Labelled Cell Count 308 175 415 372 515 823 1108 846 961 696 1035 252 249 214 140 141 162 232 122 340 251 290 435


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.8 7.5 10.5 10.4 9.4 8.0 8.3 7.8 7.9 7.7 8.0 9.5 9.5 10.1 8.5 9.7 11.4 11.7 12.3 12.5
Std Deviation (1e-4) 3.8 1.6 2.3 2.5 2.3 2.0 2.2 2.0 2.3 2.3 2.2 2.3 2.3 2.5 1.9 2.6 2.8 2.5 2.1 2.3
Intensity Change (Log2) -0.01 -0.15 -0.39 -0.34 -0.43 -0.4 -0.44 -0.39 -0.14 -0.14 -0.05 -0.29 -0.11 0.12 0.16 0.23 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.6 0.8 -0.1 0.2 -0.2 0 0.2 0 2.9 2.9 4.5 0 0 4.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -1.2 1.2 1.0 1.6 0 -0.5 -0.5 -1.0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.0 -2.4 6.3 3.4 9.6 7.6 8.2 8.1 -4.9 -4.9 -4.5 -2.5 -1.9 -1.9
Nucleus 1.9 1.4 0.9 1.2 -0.2 1.8 1.7 0.8 2.2 2.2 2.6 0 0 0
Nuclear Periphery 0.1 -1.9 -2.9 -6.7 -5.1 -5.9 -5.6 -5.5 -0.3 -0.3 -3.0 -2.0 -3.6 -3.8
Nucleolus 1.0 2.8 4.3 4.5 4.4 4.5 4.4 7.0 -3.2 -3.2 -3.9 -1.0 -0.7 1.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.6 -0.9 -3.6 -3.0 -6.1 -6.6 -5.9 -6.5 1.6 1.4 2.1 1.6 3.2 1.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.9405 7.5887 7.5501 7.095 6.8585 7.0226 7.3003 8.7797 7.616 7.0453 6.3028 7.4224 7.4377 7.9676 7.2414 6.9452 6.8572 7.3882
Actin 0.0157 0.0001 0.0096 0.0174 0.0003 0.0016 0.0208 0.0002 0.0238 0.0003 0.0013 0.0004 0.0809 0.0017 0.0326 0.0237 0.005 0.0002
Bud 0.0002 0 0.0001 0.0001 0 0 0.0007 0 0.0002 0 0 0 0.0011 0.0001 0.0003 0.0002 0 0
Bud Neck 0.0006 0 0.0001 0.0002 0 0.0001 0.0028 0 0.0002 0 0.0001 0 0.0007 0 0.0009 0.0004 0 0
Bud Periphery 0.0007 0.0001 0.0003 0.0002 0.0001 0 0.0021 0.0001 0.0004 0.0001 0.0001 0.0001 0.0046 0.0004 0.0008 0.0005 0.0002 0
Bud Site 0.0005 0 0.0001 0.0002 0 0.0002 0.0054 0 0.0109 0 0 0 0.001 0.0001 0.0188 0.0018 0 0
Cell Periphery 0.0001 0 0 0.0001 0 0 0.0007 0 0.0003 0 0 0 0.0006 0.0001 0.0003 0.0001 0 0
Cytoplasm 0.0003 0 0.0001 0.0016 0 0.0001 0.0107 0.0181 0.0015 0 0.0002 0.0004 0.0021 0.0001 0.0043 0.0001 0 0
Cytoplasmic Foci 0.002 0 0.0002 0.0029 0.0001 0.0019 0.0144 0.0002 0.0045 0.0006 0.02 0.0002 0.0017 0.0002 0.0023 0.0077 0.0003 0
Eisosomes 0.0003 0 0.0001 0.0001 0 0 0.0009 0 0.0002 0 0 0 0.0016 0.0001 0.0006 0.0001 0.0001 0
Endoplasmic Reticulum 0.0017 0.0005 0.0008 0.0023 0.0005 0.0002 0.0119 0.0022 0.0011 0.0006 0.0006 0.0019 0.02 0.002 0.006 0.0015 0.0006 0.0002
Endosome 0.0023 0.0002 0.0013 0.0037 0.0006 0.0005 0.0337 0.0017 0.0016 0.0015 0.0075 0.0077 0.0312 0.0009 0.0053 0.0014 0.0015 0.0001
Golgi 0.0019 0.0001 0.0006 0.0013 0.0003 0.0006 0.0077 0.0001 0.0032 0.002 0.0085 0.0006 0.0086 0.0018 0.0027 0.003 0.0017 0.0001
Lipid Particles 0.0117 0.0013 0.0019 0.0024 0.003 0.0046 0.0551 0.0013 0.0024 0.0458 0.0462 0.0015 0.0064 0.0404 0.0093 0.0081 0.0078 0.0008
Mitochondria 0.0084 0.0009 0.0018 0.0025 0.002 0.0012 0.011 0.0033 0.0139 0.0013 0.0188 0.0114 0.0358 0.0439 0.0303 0.0055 0.0061 0.0006
None 0.0008 0.0001 0.0008 0.0045 0.0002 0.0001 0.0117 0.0004 0.0003 0.0001 0.0002 0.0001 0.0126 0.0004 0.0193 0.0005 0.0003 0.0001
Nuclear Periphery 0.891 0.9497 0.9184 0.9117 0.9411 0.9565 0.7006 0.859 0.8382 0.8998 0.8437 0.9452 0.6799 0.8041 0.7893 0.8039 0.9333 0.96
Nucleolus 0.0009 0.0005 0.0004 0.0005 0.0031 0.0003 0.0132 0.001 0.0005 0.0005 0.0012 0.0003 0.0019 0.0005 0.001 0.0165 0.0012 0.0006
Nucleus 0.0083 0.0066 0.0203 0.0054 0.0159 0.0068 0.0255 0.0458 0.0402 0.0168 0.0123 0.0118 0.0121 0.0423 0.01 0.0058 0.0088 0.0205
Peroxisomes 0.0022 0 0.0003 0.0008 0.0001 0.0061 0.009 0 0.0036 0.0003 0.0087 0.0001 0.0014 0.0002 0.005 0.0221 0.0005 0
Punctate Nuclear 0.0023 0.0003 0.0007 0.0065 0.0009 0.0024 0.0117 0.0058 0.0005 0.0009 0.002 0.0003 0.0038 0.0004 0.002 0.0455 0.0018 0.0004
Vacuole 0.0011 0.0006 0.0005 0.0005 0.0006 0.0001 0.0061 0.0023 0.0007 0.0004 0.001 0.0004 0.0041 0.001 0.0013 0.0007 0.0003 0.0002
Vacuole Periphery 0.0472 0.039 0.0416 0.0352 0.0311 0.0166 0.0443 0.0585 0.0519 0.0289 0.0275 0.0174 0.088 0.0592 0.0577 0.0508 0.0302 0.0162

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.6079 29.4543 29.2265 37.6733 42.4713 29.1015 37.9191 38.4477 30.3176 40.3916
Translational Efficiency 1.2546 1.573 1.3138 0.7081 0.906 1.4912 1.0151 0.9522 1.1312 1.0994

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency