Standard name
Description Protein of unknown function; may have a role in lipid metabolism, based on localization to lipid droplets; predicted to be palmitoylated

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.91 0.97 0.94 0.93 0.89 0.78 0.74 0.74 0.54 0.6 0.42 0.5 0.97 0.98 0.99 0.89 0.87 0.82 0.64 0.74 0.47 0.77 0.78 0.69
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.05 0.19 0.09 0.05 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0 0 0.16 0.11 0.33 0.44 0.4 0.59 0.6 0.71 0.71 0 0 0 0 0 0 0.12 0 0.17 0.05 0.05 0.09
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.11 0.11 0 0.09 0.05 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 0 0 0 0 0 0 0 0 0 1 0 0 4 2 6 3 4 5 2 3 2
Bud 1 2 3 0 1 2 4 4 5 4 1 3 1 1 0 1 0 1 0 2 1 1 5 0
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 6 1 2 1 4
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cell Periphery 0 3 1 1 1 0 2 0 1 1 1 2 0 3 3 0 2 2 0 1 0 0 1 0
Cytoplasm 172 276 143 99 125 108 156 137 95 98 66 84 333 460 384 283 140 161 134 238 77 139 207 175
Endoplasmic Reticulum 6 9 4 2 0 5 1 4 2 4 2 1 7 3 2 7 6 4 28 15 31 16 12 16
Endosome 0 0 3 0 3 1 4 1 0 0 1 1 0 4 1 9 1 14 2 2 6 1 6 4
Golgi 0 1 0 0 1 0 0 0 0 1 0 0 0 1 0 2 0 3 0 2 2 0 0 0
Mitochondria 20 3 4 17 15 46 94 75 104 99 111 120 0 0 0 2 3 2 25 14 27 9 14 22
Nucleus 0 1 0 1 0 0 2 1 1 1 0 1 0 0 0 0 1 2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 1 1 1 1 0 0 0 1 0 1 0 0 0 0 1 1 1
Nucleolus 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 2 0 2 1 0 0 0 1 1
Vac/Vac Membrane 2 0 1 4 4 4 6 3 7 2 2 7 3 3 0 20 17 22 5 29 8 2 7 17
Unique Cell Count 188 284 152 106 140 138 212 186 176 164 157 168 342 468 386 319 161 197 210 321 167 180 268 254
Labelled Cell Count 201 297 159 124 151 168 270 227 218 212 185 220 345 475 391 330 173 219 210 321 167 180 268 254


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 5.5 5.1 4.8 5.2 4.2 3.7 3.9 3.8 3.5 3.7 3.2 5.5 5.4 5.4 7.7 9.3 9.2 4.9 5.0 5.9
Std Deviation (1e-4) 0.5 1.0 0.9 1.3 2.0 1.1 1.2 1.2 1.5 2.3 1.4 0.9 1.1 1.4 1.4 1.6 2.0 1.8 1.3 1.3 1.5
Intensity Change (Log2) -0.1 0.03 -0.27 -0.46 -0.39 -0.44 -0.54 -0.46 -0.66 0.1 0.08 0.09 0.59 0.87 0.85 -0.06 -0.02 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 -1.5 -3.9 -5.0 -5.0 -8.1 -7.2 -9.8 -8.7 1.8 2.8 4.0 -1.9 -2.1 -3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.8 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.8 3.8 3.9


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Anr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available