TheCellVision

IXR1

Standard name	IXR1 ORF YKL032C Aliases DNA-binding transcription repressor IXR1, ORD1
Human Ortholog
Description	Transcriptional repressor that regulates hypoxic genes during normoxia; involved in the aerobic repression of genes such as COX5b, TIR1, and HEM13; binds DNA intrastrand cross-links formed by cisplatin; HMG (high mobility group box) domain containing protein which binds and bends cisplatin-modified DNA, blocking excision repair; IXR1 has a paralog, ABF2, that arose from the whole genome duplication

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Scale images

IXR1

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	–	0	0	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0	0	0	0	0	0	0
Bud	–	0	0	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0	0	0	0	0	0	0
Bud Neck	–	0	0	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0	0	0	0	0	0	0
Bud Site	–	0	0	0	0	0	0	–	0	0	0	0.06	–	–	–	0	0	0	–	–	–	–	–	–
Cell Periphery	–	0	0	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0	0	0	0	0	0	0
Cytoplasm	–	0	0	0	0.11	0	0	–	0	0.07	0.05	0	–	–	–	0	0	0.07	0	0	0	0	0	0.06
Endoplasmic Reticulum	–	0	0	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0	0	0	0	0	0	0
Endosome	–	0	0	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0	0	0	0	0	0	0
Golgi	–	0	0	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0	0	0	0	0	0	0
Mitochondria	–	0	0	0	0	0	0	–	0	0	0.05	0	–	–	–	0	0	0	0	0	0	0	0	0
Nucleus	–	0.93	0.79	0.91	0.81	0.73	0.76	–	0.65	0.67	0.73	0.71	–	–	–	0.89	0.78	0.74	0.85	0.85	0.78	0.58	0.73	0.67
Nuclear Periphery	–	0	0	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0	0	0	0	0	0	0
Nucleolus	–	0.22	0.43	0.2	0.12	0.41	0.27	–	0.56	0.41	0.52	0.59	–	–	–	0.16	0.17	0.16	0.13	0.09	0.15	0.35	0	0.06
Peroxisomes	–	0	0	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0	0	0	0	0	0	0
SpindlePole	–	0	0	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0	0	0	0	0	0	0
Vac/Vac Membrane	–	0	0	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0.05	0	0	0	0.07	0	0.05

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	–	0	0	0	0	0	1	–	0	0	0	0	–	–	–	0	1	0	0	0	0	0	0	0
Bud	–	0	0	0	0	0	1	–	0	0	0	0	–	–	–	0	0	0	0	0	0	0	0	0
Bud Neck	–	0	0	0	0	0	1	–	0	0	0	0	–	–	–	0	0	0	0	0	0	0	0	0
Bud Site	–	0	0	0	1	1	0	–	3	0	0	8	–	–	–	0	1	0	–	–	–	–	–	–
Cell Periphery	–	0	0	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0	0	0	0	0	0	0
Cytoplasm	–	2	1	3	15	5	9	–	5	14	4	2	–	–	–	4	9	10	0	0	1	0	0	1
Endoplasmic Reticulum	–	0	0	0	0	0	0	–	0	0	0	0	–	–	–	0	2	3	0	0	0	0	0	0
Endosome	–	0	0	0	0	1	0	–	0	0	0	0	–	–	–	0	2	4	0	2	0	0	0	0
Golgi	–	0	0	0	0	1	1	–	0	1	0	0	–	–	–	1	0	1	0	2	0	0	0	0
Mitochondria	–	3	0	1	0	3	3	–	4	5	4	2	–	–	–	0	2	1	0	0	0	0	0	0
Nucleus	–	179	33	73	113	95	163	–	93	129	55	92	–	–	–	129	156	106	52	171	51	3	10	13
Nuclear Periphery	–	0	0	0	0	1	1	–	0	1	0	0	–	–	–	0	0	0	0	0	0	0	0	0
Nucleolus	–	42	18	16	17	53	58	–	80	79	39	76	–	–	–	23	35	23	7	17	9	2	0	1
Peroxisomes	–	0	0	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0	0	0	0	0	0	0
SpindlePole	–	0	0	0	0	0	0	–	0	0	0	0	–	–	–	0	2	0	0	1	0	0	0	0
Vac/Vac Membrane	–	0	0	0	1	1	1	–	0	2	0	0	–	–	–	3	9	7	0	5	1	0	0	1
Unique Cell Count		193	42	80	139	130	214		144	193	75	129				145	200	144	61	203	66	6	15	20
Labelled Cell Count		226	52	93	147	161	239		185	231	102	180				160	219	155	61	203	66	6	15	20

Yeast GFP Assignment

Nucleus

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	–	19.7	12.3	14.9	17.9	13.9	14.7	–	11.2	14.1	13.5	11.7	–	–	–	21.1	21.6	19.4	–	–	–
Std Deviation (1e^-4)	–	5.0	3.0	5.5	8.7	6.0	5.2	–	3.4	7.3	6.2	3.7	–	–	–	6.8	7.4	5.6	–	–	–
Intensity Change (Log₂)	–	–	–	0.28	0.55	0.18	0.25	–	-0.13	0.2	0.14	-0.07	–	–	–	0.78	0.82	0.66	–	–	–

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Cortical Patches	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0
Bud	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0
Bud Neck	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0
Bud Site	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0
Cell Periphery	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0
Cytoplasm	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0
Endoplasmic Reticulum	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0
Endosome	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0
Golgi	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0
Mitochondria	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0
Nucleus	2.0	0.4	-0.7	-0.3	–	-1.7	-1.5	-0.6	-0.9	–	–	–	1.7	-0.1	-0.7
Nuclear Periphery	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0
Nucleolus	-2.7	-4.4	-0.2	-2.0	–	1.5	-0.2	0.9	1.8	–	–	–	-3.7	-3.6	-3.7
Peroxisomes	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0
SpindlePole	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0
Vacuole	0	0	0	0	–	0	0	0	0	–	–	–	0	0	0

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	14.0021	13.0088	12.5869	11.9174	11.2143	12.759	14.1226	12.4704	11.5389	11.9846	9.4056	10.7411	16.21	13.9731	12.4965	13.1453	12.8913	13.4577
Actin	0.0123	0	0.0068	0.0172	0.0007	0	0.0009	0	0.0107	0	0.0343	0.0001	0.0343	0	0.0196	0.0003	0	0.0013
Bud	0.0008	0	0.0002	0.0002	0.0002	0.0001	0.0002	0	0.0002	0	0.0008	0.0003	0.0003	0	0.0003	0.0002	0.0001	0.0002
Bud Neck	0.0026	0.0001	0.0008	0.0004	0.0004	0.0006	0.0001	0.0001	0.0001	0	0.0009	0.0007	0.0008	0	0.0006	0.0001	0.0002	0.0004
Bud Periphery	0.0016	0	0.0002	0.0005	0.0004	0.0001	0.0004	0	0.0003	0	0.0021	0.0014	0.0006	0	0.0007	0.0003	0.0002	0.0003
Bud Site	0.0062	0	0.0069	0.0005	0.0003	0.0001	0.0003	0.0001	0.0006	0	0.0011	0.0001	0.0025	0	0.0011	0.0002	0.0001	0.0001
Cell Periphery	0.0015	0	0.0003	0.0005	0.0003	0.0001	0.0004	0.0001	0.0002	0	0.0003	0.0006	0.0003	0	0.0001	0.0005	0.0002	0.0002
Cytoplasm	0.0019	0	0.0006	0.0001	0.0008	0.0001	0.0015	0	0.0001	0	0.0006	0.0002	0.0027	0	0.0002	0.0003	0	0.0011
Cytoplasmic Foci	0.0098	0	0.0058	0.004	0.0007	0.0037	0.0116	0	0.0061	0	0.0227	0.0033	0.0099	0	0.0034	0.0213	0	0.0002
Eisosomes	0.0005	0	0	0.0001	0	0	0	0	0.0002	0	0.0003	0	0.0008	0	0.0002	0	0	0
Endoplasmic Reticulum	0.0046	0	0.0004	0.0002	0.0006	0	0.0005	0	0.0001	0	0.0019	0.0003	0.002	0	0.0002	0.0001	0	0.0005
Endosome	0.0101	0	0.0062	0.0032	0.0073	0.0005	0.0087	0	0.0012	0	0.0493	0.0007	0.0071	0	0.0064	0.0008	0	0.0038
Golgi	0.0038	0	0.0038	0.012	0.0005	0.0002	0.001	0	0.0017	0	0.0199	0.0002	0.0046	0	0.0032	0.0011	0	0.0032
Lipid Particles	0.0127	0	0.0091	0.0024	0.0009	0.0046	0.0065	0	0.0131	0	0.007	0.0062	0.0051	0	0.0021	0.0236	0.0001	0.0037
Mitochondria	0.0162	0.0001	0.0051	0.0071	0.0024	0.0006	0.0026	0.0004	0.0156	0	0.0072	0.0033	0.0059	0.0003	0.0327	0.0034	0.0016	0.0027
None	0.0015	0	0.0005	0.0001	0.0006	0.0001	0.002	0	0.0002	0	0.0002	0.0002	0.0018	0	0.0001	0.0006	0	0.0001
Nuclear Periphery	0.0212	0.0011	0.0022	0.0017	0.0213	0.0003	0.0045	0.0017	0.003	0	0.0014	0.0011	0.0019	0.0004	0.0003	0.0008	0.0001	0.0035
Nucleolus	0.1369	0.0431	0.0385	0.0374	0.4267	0.2097	0.0464	0.0734	0.0615	0.0194	0.1542	0.1283	0.0534	0.0492	0.0247	0.061	0.3621	0.1198
Nucleus	0.7244	0.9552	0.905	0.8621	0.5274	0.7755	0.888	0.9235	0.8725	0.9805	0.6915	0.8416	0.8582	0.9498	0.891	0.8676	0.6341	0.8564
Peroxisomes	0.0055	0	0.0015	0.0293	0.0003	0.0019	0.0104	0	0.0104	0	0.001	0.0002	0.0038	0	0.004	0.0043	0.0001	0.0001
Punctate Nuclear	0.0097	0.0003	0.0049	0.0008	0.003	0.0011	0.0113	0.0005	0.0013	0	0.0006	0.0013	0.0026	0.0002	0.0001	0.0101	0.0006	0.0003
Vacuole	0.012	0.0001	0.0009	0.0057	0.0027	0.0004	0.0013	0.0001	0.0005	0	0.0017	0.0064	0.0008	0	0.005	0.0015	0.0002	0.0011
Vacuole Periphery	0.0042	0	0.0005	0.0144	0.0026	0.0002	0.0013	0.0002	0.0005	0	0.001	0.0035	0.0006	0.0001	0.0038	0.0019	0.0002	0.001

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	18.7591	34.6386	19.5835	26.6892	27.113	20.2855	38.3586	30.0168	26.4406	35.8384
Translational Efficiency	2.5054	2.1498	2.436	1.345	2.3186	2.3603	1.5125	1.7848	1.9762	1.8655

Hit Data

Dataset	Hit
Protein Concentration	✘
Protein Localization	✘
Gene Expression	✘
Translational Efficiency	✘

Proteome Dynamics

Micrographs

Cell Count

R1				R2				R1 & R2
WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
1087	1343	280	1480	2003	1950	263	2088	3090	3293	543	3568

Protein Abundance

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Mean	792.01	1227.67	1998.15	1522.83	1235.54	1325.97	1704.56	1625.01	1079.52	1285.88	1855.95	1582.63
Standard Deviation	142.49	306.04	346.26	349.13	260.20	277.31	280.65	352.96	309.66	293.38	348.57	354.96
Intensity Change Log ₂	—	0.632332	1.335074	0.943164	—	0.101906	0.464258	0.395307	—	0.332818	0.868844	0.634625

Localization Score (DeepLoc)

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Actin	0.000709	0.000652	0.001184	0.001118	0.000490	0.000531	0.000809	0.000761	0.000567	0.000580	0.001002	0.000909
Bud Neck	0.001763	0.002919	0.012802	0.015830	0.003771	0.005377	0.009930	0.020034	0.003064	0.004374	0.011411	0.018290
Bud Site	0.001569	0.000709	0.001387	0.002408	0.000413	0.000778	0.000862	0.001836	0.000820	0.000750	0.001133	0.002073
Cell Periphery	0.000236	0.000181	0.000024	0.000124	0.000081	0.000120	0.000034	0.000103	0.000136	0.000145	0.000029	0.000111
Cytoplasm	0.005845	0.000817	0.000020	0.001357	0.000395	0.000200	0.000155	0.001644	0.002312	0.000452	0.000086	0.001525
Cytoplasmic Foci	0.000528	0.000123	0.000369	0.001120	0.000026	0.000247	0.000167	0.000313	0.000202	0.000197	0.000271	0.000648
Eisosomes	0.000049	0.000131	0.000205	0.000045	0.000178	0.000218	0.000107	0.000041	0.000133	0.000183	0.000157	0.000042
Endoplasmic Reticulum	0.002772	0.000903	0.000442	0.000657	0.000923	0.001055	0.000527	0.000668	0.001573	0.000993	0.000483	0.000664
Endosome	0.000311	0.000130	0.000013	0.000655	0.000033	0.000348	0.000017	0.000419	0.000131	0.000259	0.000015	0.000517
Golgi	0.000262	0.000092	0.000104	0.000841	0.000031	0.000170	0.000079	0.000382	0.000112	0.000138	0.000092	0.000572
Lipid Particles	0.000078	0.000066	0.000019	0.000233	0.000005	0.000044	0.000012	0.000024	0.000031	0.000053	0.000016	0.000111
Mitochondria	0.000842	0.000868	0.002809	0.003100	0.000715	0.001024	0.001587	0.002530	0.000760	0.000960	0.002217	0.002767
Mitotic Spindle	0.000179	0.000705	0.000214	0.004280	0.000069	0.000745	0.003648	0.002548	0.000108	0.000729	0.001878	0.003267
None	0.015682	0.004864	0.000542	0.002214	0.000894	0.000679	0.001096	0.001354	0.006096	0.002386	0.000810	0.001710
Nuclear Periphery	0.000030	0.000050	0.000042	0.000505	0.000026	0.000100	0.000054	0.000445	0.000027	0.000080	0.000048	0.000470
Nuclear Periphery Foci	0.000482	0.000611	0.000007	0.000549	0.000030	0.000092	0.000012	0.000151	0.000189	0.000304	0.000009	0.000316
Nucleolus	0.065055	0.060784	0.136188	0.083657	0.075897	0.057031	0.073954	0.069902	0.072083	0.058561	0.106045	0.075608
Nucleus	0.900780*	0.922024*	0.824989*	0.877302*	0.913781*	0.928157*	0.901059*	0.894573*	0.909207*	0.925656*	0.861833*	0.887409*
Peroxisomes	0.001415	0.002090	0.017884	0.001635	0.001645	0.001461	0.005445	0.000985	0.001564	0.001718	0.011859	0.001255
Vacuole	0.001129	0.000802	0.000452	0.001652	0.000338	0.000889	0.000289	0.000703	0.000616	0.000853	0.000373	0.001097
Vacuole Periphery	0.000284	0.000478	0.000303	0.000720	0.000259	0.000736	0.000158	0.000583	0.000268	0.000631	0.000233	0.000640

Localization Changes (T-score)

	R1					R2					R1 & R2
	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14
Actin	0.79	-6.25	-3.63	-3.91	-0.24	-1.84	-8.12	-8.32	-7.11	0.20	-0.30	-10.62	-6.71	-6.29	0.37
Bud Neck	-5.86	-12.19	-17.37	-16.15	-4.29	-4.79	-9.34	-17.86	-15.98	-9.42	-5.97	-14.74	-24.23	-22.10	-9.74
Bud Site	2.13	-0.05	-2.07	-3.75	-2.33	-2.51	-4.66	-5.08	-3.87	-3.00	0.44	-3.32	-5.16	-5.38	-3.63
Cell Periphery	0.75	5.18	2.46	0.87	-5.17	-1.19	1.67	-0.74	0.47	-2.69	-0.28	4.85	0.84	0.97	-5.03
Cytoplasm	3.35	3.89	2.71	-1.75	-3.74	0.70	0.84	-2.36	-2.94	-3.00	3.33	4.00	0.99	-3.43	-4.56
Cytoplasmic Foci	1.65	0.76	-0.92	-2.42	-1.73	-1.52	-2.28	-1.61	-0.16	-0.02	0.49	-0.66	-1.67	-2.12	-1.45
Eisosomes	-5.69	-13.24	0.92	5.92	13.52	-1.66	5.42	12.82	8.20	11.12	-3.18	-3.30	12.57	9.92	16.30
Endoplasmic Reticulum	8.72	11.84	10.58	1.70	-4.79	-1.29	5.87	4.00	3.39	-2.55	5.75	13.32	11.03	3.74	-5.14
Endosome	1.68	2.79	-0.81	-2.05	-2.72	-2.25	0.94	-1.78	-0.08	-1.83	-1.05	2.92	-2.04	-1.19	-3.13
Golgi	1.70	1.39	-1.29	-1.88	-1.78	-1.28	-4.33	-2.30	-1.10	-1.74	-0.24	-0.05	-2.36	-2.17	-2.40
Lipid Particles	0.39	2.62	-1.30	-1.41	-1.89	-2.06	-3.27	-3.69	0.70	-1.61	-1.08	1.49	-1.72	-1.21	-2.02
Mitochondria	-0.22	-9.71	-11.02	-11.88	-1.89	-2.25	-9.35	-11.71	-8.09	-5.45	-2.17	-13.31	-16.31	-13.34	-4.31
Mitotic Spindle	-1.02	-1.30	-4.73	-4.02	-4.58	-1.86	-1.25	-5.19	-4.35	-0.39	-2.06	-1.32	-7.02	-5.92	-1.98
None	4.24	6.61	5.79	2.14	-4.74	1.50	-0.76	-3.87	-6.02	-1.42	3.94	6.33	5.06	1.01	-4.67
Nuclear Periphery	-2.02	-3.80	-6.78	-6.49	-6.54	-2.66	-3.33	-9.02	-6.86	-7.88	-3.09	-4.51	-11.11	-9.43	-10.27
Nuclear Periphery Foci	-0.25	3.76	-0.42	-0.04	-1.92	-1.94	1.34	-3.96	-1.97	-4.49	-0.57	3.93	-1.24	-0.29	-2.61
Nucleolus	1.03	-7.81	-6.58	-7.25	4.13	5.32	-0.20	-1.79	-6.46	-0.77	4.98	-6.00	-5.37	-9.67	3.11
Nucleus	-3.90	7.31	6.62	10.47	-3.24	-3.67	2.16	8.29	11.24	2.24	-5.17	7.36	10.86	15.37	-1.45
Peroxisomes	-0.92	-7.49	-0.72	0.35	7.43	1.24	-4.61	2.49	1.47	4.99	-0.34	-8.50	0.52	1.06	8.59
Vacuole	1.03	2.79	-0.95	-1.57	-2.37	-2.37	-0.91	-1.99	-0.06	-1.68	-1.25	1.68	-2.18	-1.17	-2.86
Vacuole Periphery	-1.10	-0.71	-2.30	-0.89	-2.17	-2.45	3.19	-2.46	0.69	-3.10	-2.68	0.75	-3.39	0.04	-3.60