Standard name
Human Ortholog
Description tRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD2 has a paralog, TCD1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.09 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0.19 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.23 0.43 0.6 0.3 0.22 0.14 0.05 0.1 0.05 0.12 0.12 0.1
Mitochondria 0.95 0.94 0.92 0.9 0.85 0.91 0.89 0.88 0.88 0.9 0.93 0.93 0.82 0.63 0.7 0.14 0.1 0 0.86 0.76 0.85 0.72 0.67 0.72
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0 0 0 0.1 0.12 0.13 0.1 0.1 0.07 0.1 0 0 0 0.07 0.06 0 0 0 0 0 0 0
Nucleolus 0.09 0.06 0 0 0.08 0.16 0.15 0.12 0.09 0.09 0.08 0.07 0 0 0 0.07 0.06 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.1 0 0 0.07 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.13 0.07 0 0.06 0 0 0 0.05 0.06 0.05 0.08 0.17 0.27 0.19 0.28 0.32 0.33 0 0.06 0 0.09 0.1 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 1 0 0 0 1 1 0 1 1 0 2 0 2 3 1 2 4 1 3 2 9 8
Bud 1 1 0 7 2 4 9 5 11 9 9 10 1 1 2 1 3 0 0 0 0 1 2 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1
Bud Site 1 1 1 0 2 3 3 3 1 7 2 2 0 0 0 0 0 1
Cell Periphery 4 4 10 2 1 3 3 1 1 2 2 3 7 20 12 4 6 2 0 0 0 0 0 0
Cytoplasm 0 0 5 12 21 11 13 10 16 12 7 2 5 10 5 4 6 8 0 0 0 0 1 0
Endoplasmic Reticulum 2 1 0 0 2 0 5 4 2 3 3 3 3 0 4 2 5 2 1 0 0 1 1 2
Endosome 1 0 1 0 4 1 2 9 4 1 3 7 0 6 3 2 2 0 4 2 1 4 6 4
Golgi 4 3 10 2 7 1 0 3 0 0 0 0 51 155 208 13 21 6 17 13 13 26 44 32
Mitochondria 315 118 247 155 238 264 369 378 373 560 351 542 183 224 243 6 10 1 286 98 239 157 251 227
Nucleus 0 1 0 0 1 1 3 4 6 2 1 4 1 1 0 0 3 1 0 0 0 1 3 0
Nuclear Periphery 19 3 4 4 9 28 48 56 41 60 28 57 7 2 2 3 6 1 2 0 0 0 2 1
Nucleolus 31 8 9 3 22 45 62 52 39 55 29 43 4 2 0 3 6 2 0 1 0 0 1 2
Peroxisomes 0 0 9 1 5 1 4 0 4 2 3 4 4 3 2 2 6 4 4 0 19 2 6 4
SpindlePole 0 1 0 0 1 0 2 4 1 6 1 5 1 0 0 1 1 1 0 0 0 0 1 0
Vac/Vac Membrane 34 16 19 1 18 10 11 19 21 36 19 45 37 98 67 12 31 14 6 7 2 18 36 24
Unique Cell Count 330 126 269 172 281 289 416 432 425 619 378 580 223 357 345 43 96 42 333 129 283 220 376 317
Labelled Cell Count 414 158 316 187 333 372 535 549 520 756 459 727 306 522 550 56 108 45 333 129 283 220 376 317


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.0 13.9 12.3 10.8 10.0 8.5 8.2 8.3 8.1 7.8 7.3 7.5 12.9 13.0 12.1 12.0 11.7 11.2 13.7 13.4 14.9
Std Deviation (1e-4) 1.9 3.3 2.8 2.7 2.4 2.6 2.1 2.5 2.4 2.3 2.0 1.9 2.5 2.2 2.1 3.3 3.0 3.8 2.8 3.2 3.0
Intensity Change (Log2) -0.19 -0.3 -0.53 -0.59 -0.56 -0.61 -0.66 -0.75 -0.72 0.07 0.08 -0.02 -0.04 -0.07 -0.13 0.16 0.12 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -2.8 -2.1 -2.8 0 0 0 0 0 -0.3 1.1 -0.2 0 0 0
Cytoplasm 2.7 3.1 1.4 1.0 0.4 1.4 0.1 0 0 0 0.8 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 -0.8 -2.9 0 0 0 0 0 0 6.4 11.2 14.5 0 5.5 0
Mitochondria -0.6 -2.6 -0.2 -1.3 -1.8 -1.7 -0.6 0.5 0.9 -3.2 -8.3 -6.5 -12.1 -15.0 -13.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 1.3 4.2 4.9 5.3 4.3 4.3 3.4 4.4 0 0 0 0 0 0
Nucleolus 0 2.3 4.9 4.8 4.0 2.9 2.9 2.3 2.3 -1.1 0 0 0 0 0
Peroxisomes 0 -1.2 0 -2.2 0 -2.3 0 0 0 -1.1 -2.3 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.2 -0.3 -1.9 -2.8 -1.5 -1.2 -0.7 -1.1 0.4 3.3 6.5 4.4 0 6.2 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.7795 8.9542 9.3934 9.321 8.6666 9.196 7.559 8.3157 8.0436 7.2683 7.411 7.8881 6.372 7.2899 6.8466 6.6322 5.1987 7.0715
Actin 0.0062 0.0231 0.0195 0.0001 0.0001 0.0004 0.0468 0.0071 0.0088 0.0122 0.0567 0.002 0.0429 0.0175 0.0011 0.0148 0.0789 0.0014
Bud 0.0005 0.002 0.0031 0.0001 0.0001 0 0.0015 0.0008 0.0006 0.0027 0.0004 0.0001 0.0006 0.0003 0.0002 0.0014 0.0014 0.0001
Bud Neck 0.0004 0.0008 0.001 0 0 0.0001 0.0011 0.0002 0.0002 0.0002 0.0001 0.0001 0.0014 0.0006 0.0004 0.0001 0.0015 0
Bud Periphery 0.0009 0.0028 0.0034 0.0005 0.0009 0.0002 0.0041 0.0012 0.0012 0.0061 0.0018 0.0004 0.001 0.0005 0.0006 0.0026 0.0031 0.0001
Bud Site 0.0014 0.0213 0.0038 0.0001 0 0 0.0061 0.0023 0.0031 0.0009 0.0002 0.0002 0.0037 0.003 0.0003 0.0008 0.0059 0
Cell Periphery 0.0003 0.0013 0.0006 0.0003 0.0002 0.0001 0.0008 0.0006 0.0003 0.0013 0.0003 0.0001 0.0004 0.0015 0.0006 0.0001 0.0006 0.0001
Cytoplasm 0.0006 0.0003 0.0006 0 0 0.0001 0.0024 0.0019 0.0003 0.0003 0.0001 0.0001 0.01 0 0 0.0001 0.0027 0.0002
Cytoplasmic Foci 0.0066 0.0075 0.0065 0.0001 0.0001 0.0005 0.0069 0.0032 0.003 0.0012 0.0012 0.0011 0.0117 0.0023 0.0004 0.006 0.0446 0.0006
Eisosomes 0.0002 0.0004 0.0015 0 0 0.0001 0.0009 0.0004 0.0003 0.0002 0.0001 0.0002 0.0011 0.0005 0.0001 0.0004 0.0009 0.0001
Endoplasmic Reticulum 0.0004 0.0002 0.0006 0 0 0.0001 0.0044 0.0007 0.0003 0.0004 0.0001 0.0007 0.0031 0.0001 0.0001 0 0.0041 0.0003
Endosome 0.0304 0.0205 0.0078 0.002 0.0005 0.006 0.0276 0.0134 0.0056 0.0105 0.0036 0.0076 0.0191 0.0019 0.005 0.0067 0.0351 0.004
Golgi 0.0313 0.0419 0.0102 0.0008 0.0007 0.0097 0.0185 0.0245 0.0134 0.0179 0.0034 0.0175 0.0129 0.0028 0.0053 0.0332 0.0198 0.0046
Lipid Particles 0.0074 0.0212 0.0074 0.0013 0.0003 0.0024 0.0152 0.0021 0.0026 0.001 0.0031 0.0026 0.0204 0.0138 0.0038 0.0023 0.0252 0.0008
Mitochondria 0.8907 0.8197 0.9081 0.979 0.9879 0.9616 0.8182 0.9111 0.9413 0.9374 0.8752 0.9587 0.8273 0.9339 0.9697 0.9241 0.6471 0.9836
None 0.0001 0.0001 0.0106 0 0 0.0021 0.0016 0.0004 0.0002 0.0001 0 0.0001 0.0088 0.0001 0 0.0001 0.0036 0.0001
Nuclear Periphery 0.0001 0.0002 0.0004 0 0 0.0003 0.0068 0.0002 0.0019 0.0003 0.0002 0.0024 0.0058 0.0005 0 0 0.0208 0.0013
Nucleolus 0.0003 0.0004 0.0004 0.0003 0 0.0033 0.0016 0.004 0.0009 0.0002 0.0002 0.0001 0.0009 0.0004 0.0007 0.0001 0.0063 0.0001
Nucleus 0.0002 0.0002 0.0003 0.0001 0 0.0019 0.0025 0.0015 0.0017 0.0002 0.0002 0.0002 0.0043 0.0002 0.0002 0.0001 0.0071 0.0007
Peroxisomes 0.0083 0.011 0.0079 0.0007 0.0017 0.0012 0.0104 0.0029 0.0025 0.0008 0.0064 0.0011 0.0132 0.0044 0.0012 0.0044 0.0049 0.0005
Punctate Nuclear 0.0001 0 0.0005 0 0 0.0021 0.0027 0.0002 0.0024 0 0 0.001 0.0069 0.0026 0 0 0.0408 0.0001
Vacuole 0.0035 0.0079 0.0022 0.0079 0.002 0.0017 0.0083 0.0096 0.0025 0.0029 0.013 0.0006 0.0017 0.0038 0.0053 0.0007 0.0148 0.0004
Vacuole Periphery 0.0101 0.017 0.0035 0.0065 0.0051 0.0062 0.0118 0.0117 0.0073 0.0032 0.0337 0.0032 0.0028 0.0093 0.0049 0.0021 0.0309 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.324 32.2921 29.9526 20.9105 14.37 24.6159 23.1893 27.0453 22.3526 22.6531
Translational Efficiency 0.8874 0.7958 0.7617 1.1198 1.4723 0.695 0.9765 0.7106 0.7918 0.7499

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
250 234 1573 989 1502 1478 330 66 1752 1712 1903 1055

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1138.25 1233.76 1342.50 1191.46 1078.40 1174.16 1354.69 1198.61 1086.94 1182.31 1344.61 1191.91
Standard Deviation 214.43 214.74 221.99 203.91 197.13 188.82 237.04 232.87 200.78 193.65 224.72 205.85
Intensity Change Log 2 0.116244 0.238105 0.065913 0.122737 0.329070 0.152470 0.119406 0.283076 0.108672

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001226 0.001248 0.001729 0.004335 0.000470 0.003010 0.003014 0.005843 0.000578 0.002769 0.001952 0.004429
Bud Neck 0.000319 0.003311 0.001775 0.003146 0.000600 0.000742 0.010411 0.010264 0.000560 0.001093 0.003273 0.003591
Bud Site 0.002416 0.008675 0.004122 0.035051 0.000999 0.002757 0.018229 0.107576* 0.001202 0.003566 0.006569 0.039588
Cell Periphery 0.002938 0.000272 0.000249 0.001521 0.000214 0.000271 0.000859 0.001004 0.000603 0.000271 0.000355 0.001489
Cytoplasm 0.002274 0.000835 0.000629 0.004686 0.000111 0.000291 0.001336 0.012522 0.000420 0.000365 0.000751 0.005176
Cytoplasmic Foci 0.012365 0.018981 0.013949 0.021887 0.007527 0.010357 0.024010 0.056274 0.008217 0.011535 0.015694 0.024038
Eisosomes 0.000096 0.000391 0.000059 0.000103 0.000057 0.000062 0.000096 0.000153 0.000062 0.000107 0.000066 0.000106
Endoplasmic Reticulum 0.005057 0.000424 0.000280 0.001394 0.000159 0.000269 0.000841 0.002477 0.000858 0.000290 0.000377 0.001461
Endosome 0.008398 0.014741 0.009780 0.029866 0.004187 0.010529 0.045826 0.072622 0.004788 0.011105 0.016031 0.032541
Golgi 0.065949 0.027205 0.031524 0.116565* 0.010735 0.043966 0.038554 0.158653* 0.018614 0.041675 0.032743 0.119198*
Lipid Particles 0.002256 0.001187 0.002146 0.000536 0.001580 0.001471 0.007161 0.000838 0.001676 0.001432 0.003015 0.000554
Mitochondria 0.859340* 0.864599* 0.856227* 0.635636* 0.951430* 0.889946* 0.699396* 0.360921* 0.938289* 0.886482* 0.829031* 0.618450*
Mitotic Spindle 0.001077 0.002979 0.009471 0.031643 0.000619 0.001577 0.037820 0.028516 0.000684 0.001769 0.014387 0.031448
None 0.001309 0.001298 0.001069 0.000892 0.001050 0.000560 0.000787 0.000558 0.001087 0.000661 0.001020 0.000871
Nuclear Periphery 0.000209 0.001243 0.001693 0.000344 0.000208 0.000140 0.002410 0.000302 0.000209 0.000291 0.001817 0.000341
Nuclear Periphery Foci 0.000286 0.001334 0.000681 0.000494 0.000187 0.000134 0.002030 0.000349 0.000201 0.000298 0.000915 0.000485
Nucleolus 0.000079 0.001568 0.000287 0.000293 0.000246 0.000147 0.002093 0.000251 0.000222 0.000342 0.000600 0.000291
Nucleus 0.000077 0.000338 0.000125 0.000400 0.000091 0.000061 0.000844 0.001070 0.000089 0.000099 0.000250 0.000442
Peroxisomes 0.000732 0.008092 0.004555 0.005316 0.004087 0.004645 0.019312 0.016977 0.003609 0.005116 0.007114 0.006046
Vacuole 0.005827 0.019508 0.019751 0.045331 0.004375 0.012420 0.041019 0.117044 0.004582 0.013389 0.023439 0.049818
Vacuole Periphery 0.027769 0.021771 0.039899 0.060563 0.011068 0.016645 0.043952 0.045786 0.013451 0.017346 0.040601 0.059638

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.05 -1.23 -3.59 -3.45 -2.93 -3.40 -4.00 -1.98 -1.01 -1.02 -3.38 -4.53 -4.80 -1.65 -2.93
Bud Neck -2.92 -6.81 -6.39 0.08 -2.88 -0.74 -4.34 -3.76 -3.70 0.04 -2.42 -6.12 -6.65 -5.21 -0.59
Bud Site -1.83 -1.38 -9.65 -5.82 -9.30 -2.78 -4.31 -4.14 -4.07 -3.41 -3.28 -5.42 -11.07 -10.25 -9.21
Cell Periphery 2.67 2.69 1.38 -4.65 -4.74 -1.14 -1.80 -3.24 -3.02 -0.29 2.21 1.46 -3.08 -4.93 -4.39
Cytoplasm 0.84 0.96 -1.55 -3.94 -4.26 -4.52 -3.28 -1.54 -1.53 -1.45 0.22 -1.03 -4.43 -4.54 -4.17
Cytoplasmic Foci -1.41 -0.58 -3.04 -0.66 -3.51 -2.09 -4.82 -4.39 -4.14 -2.86 -2.49 -5.19 -7.57 -5.78 -3.75
Eisosomes -0.98 2.48 -0.27 0.95 -1.84 -0.79 -3.69 -2.46 -2.34 -1.43 -1.06 -0.66 -1.98 0.01 -1.81
Endoplasmic Reticulum 1.47 1.51 1.16 -4.01 -5.66 -3.08 -5.59 -1.82 -1.73 -1.27 1.25 1.06 -1.23 -5.88 -5.40
Endosome -2.07 -0.77 -8.51 -4.72 -9.75 -5.72 -7.65 -4.33 -3.92 -1.54 -6.04 -8.49 -12.98 -9.62 -6.94
Golgi 3.52 3.38 -4.26 -11.27 -12.64 -10.09 -5.73 -5.26 -4.03 -4.19 -7.05 -5.23 -15.51 -11.40 -13.23
Lipid Particles 0.98 0.09 1.61 1.85 4.97 0.17 -2.09 1.29 0.74 2.31 0.43 -2.31 3.62 1.68 4.55
Mitochondria -0.23 0.20 11.37 11.65 16.88* 9.19 13.58 12.65 11.30 6.85 7.82 15.20 26.47 21.24 16.32*
Mitotic Spindle -1.62 -5.68 -8.84 -8.01 -6.09 -2.34 -6.15 -2.03 -1.97 0.43 -2.79 -8.59 -9.23 -8.88 -4.80
None 0.04 1.32 2.32 1.57 3.26 7.69 2.72 4.27 0.01 1.83 6.09 0.99 3.24 -3.80 2.98
Nuclear Periphery -1.50 -4.97 -1.62 1.31 4.59 1.55 -5.64 -0.66 -1.15 5.07 -0.81 -6.40 -2.43 -0.53 5.83
Nuclear Periphery Foci -1.38 -2.13 -1.37 1.05 1.02 1.24 -4.25 -1.47 -1.98 3.74 -0.88 -6.23 -3.30 -1.69 2.70
Nucleolus -1.35 -3.19 -1.34 1.14 -0.08 0.67 -2.36 -0.11 -0.97 2.35 -0.61 -2.19 -0.43 0.19 1.51
Nucleus -1.15 -1.03 -1.19 -0.66 -1.10 0.70 -4.74 -2.14 -2.19 -0.98 -0.21 -3.55 -1.34 -1.31 -1.02
Peroxisomes -4.13 -4.76 -5.63 1.41 -0.68 -0.41 -3.97 -1.91 -1.84 0.05 -1.26 -2.93 -2.19 -0.84 0.73
Vacuole -3.33 -6.62 -11.20 -5.26 -7.30 -5.10 -8.20 -4.75 -4.41 -3.17 -5.97 -12.10 -13.18 -10.02 -7.22
Vacuole Periphery 0.88 -2.02 -4.91 -7.03 -4.61 -3.06 -5.96 -4.48 -3.74 -0.09 -2.11 -10.53 -12.15 -11.21 -4.52