Standard name
Description Endosomal SNARE related to mammalian syntaxin 8

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.93 1.0 0.98 0.97 0.88 0.83 0.7 0.73 0.65 0.66 1.0 0.99 0.99 0.94 0.97 0.96 0.92 0.89 0.93 0.92 0.92 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0.13 0.08 0.31 0.25 0.34 0.35 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.18 0.14 0.1 0.07 0.07 0 0 0 0.05 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0
Bud 0 0 0 0 0 3 5 9 9 14 6 0 1 0 0 0 1 0 0 1 2 4 2
Bud Neck 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 1 0 0 1 2 1 8 5 7 6 11 1 0 5 6 6 9 0 0 0 0 0 0
Cytoplasm 76 69 48 242 361 255 505 311 354 272 321 211 561 601 224 195 243 80 81 78 269 396 437
Endoplasmic Reticulum 0 1 0 0 0 1 0 1 0 0 0 1 1 2 7 9 6 0 0 0 2 2 2
Endosome 0 0 0 0 0 0 2 0 0 0 0 0 1 0 0 0 0 1 1 0 1 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 1 0 1 3 2
Mitochondria 3 4 0 2 4 39 46 140 121 145 172 0 0 1 5 1 1 0 1 0 0 2 0
Nucleus 0 0 0 1 2 5 11 18 18 21 17 0 1 0 7 4 2 1 0 0 0 3 0
Nuclear Periphery 1 2 0 0 0 0 2 4 1 1 2 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1
Vac/Vac Membrane 2 1 0 9 14 12 109 62 48 30 35 0 4 15 12 9 14 2 3 0 6 5 3
Unique Cell Count 81 74 48 248 373 289 611 445 488 421 485 211 565 610 239 202 254 88 91 84 292 431 459
Labelled Cell Count 83 77 48 255 383 316 688 552 561 491 565 213 570 626 262 224 276 88 91 84 292 431 459


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 5.6 6.4 6.2 7.0 5.1 6.1 5.3 5.4 5.2 5.4 7.0 7.2 7.4 8.0 8.4 8.5 6.1 6.4 6.5
Std Deviation (1e-4) 0.8 0.7 2.0 1.3 1.2 1.3 1.1 1.2 1.0 0.9 1.0 1.0 1.2 1.3 1.1 1.1 1.2 1.1 1.4 1.4
Intensity Change (Log2) -0.04 0.12 -0.32 -0.06 -0.26 -0.24 -0.3 -0.23 0.14 0.17 0.21 0.32 0.39 0.41 -0.06 -0.01 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.1 -1.3 -2.5 -3.2 -4.5 -4.2 -5.0 -4.8 0 -0.6 -0.8 -1.8 -1.3 -1.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.7 0 4.6 3.9 4.9 5.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.2 2.8 0 0 0 0 0 0 0 0 0