Standard name
Human Ortholog
Description Putative protein of unknown function; predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – – – – – 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.94 – – – – – 0.82 0.77 0.72 0.57 0.58 0.89 0.96 0.97 0.94 0.88 0.94 0.91 0.87 0.8 0.84 0.82 0.7
Endoplasmic Reticulum 0 0 – – – – – 0 0 0 0 0 0.09 0 0 0 0.05 0.06 0 0 0.08 0 0 0.09
Endosome 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – – – – – 0.14 0.32 0.24 0.48 0.52 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – – – – – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 – – – – – 0 0 1 0 1 1 1 1 0 1 0 0 0 0 0 0 2
Bud 0 0 – – – – – 1 0 1 2 1 2 0 1 0 0 0 0 1 0 1 3 2
Bud Neck 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2
Bud Site 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 0 – – – – – 1 0 1 1 1 3 7 2 10 5 0 0 0 0 0 0 0
Cytoplasm 119 107 – – – – – 64 17 49 26 41 91 312 299 104 113 162 120 111 23 140 291 211
Endoplasmic Reticulum 2 2 – – – – – 1 0 1 0 3 9 8 3 3 6 10 4 4 2 4 10 27
Endosome 0 0 – – – – – 1 0 2 2 0 0 1 2 0 4 1 0 0 0 0 3 6
Golgi 0 0 – – – – – 0 0 0 0 0 0 0 0 0 2 0 0 1 0 0 0 2
Mitochondria 2 3 – – – – – 11 7 16 22 37 2 2 1 6 5 6 2 2 1 3 8 9
Nucleus 0 1 – – – – – 0 0 1 0 2 1 0 1 0 0 1 0 1 0 1 1 1
Nuclear Periphery 0 2 – – – – – 1 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 – – – – – 1 0 0 0 0 0 1 2 0 0 0 0 0 0 2 12 13
Peroxisomes 0 0 – – – – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – – – – 0 0 0 1 0 0 0 1 0 0 0 0 0 0 3 7 8
Vac/Vac Membrane 1 0 – – – – – 3 0 2 0 1 1 3 3 3 7 8 1 2 1 5 12 8
Unique Cell Count 122 114 78 22 68 46 71 102 325 309 111 129 173 133 128 29 167 357 304
Labelled Cell Count 127 116 84 25 74 55 87 110 335 316 126 143 189 133 128 29 167 357 304


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 6.6 – – – – – 6.5 5.3 5.8 5.1 5.1 7.6 7.9 7.4 7.3 8.7 9.3 8.6 8.9 8.7
Std Deviation (1e-4) 1.1 1.1 – – – – – 1.4 0.9 1.5 1.1 1.0 1.2 1.1 1.0 1.6 1.7 1.7 1.2 1.5 1.4
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.7072 6.978 6.2858 5.5155 5.3676 6.4349 5.0994 6.1255 5.8254 5.8798 4.6659 5.6711 2.2045 3.2392 2.9454 2.9657 3.1042 2.881
Actin 0.0117 0.0017 0.0137 0.0006 0.0376 0.018 0.0038 0.0113 0.0048 0.0004 0.0008 0.0068 0.0276 0.001 0.0046 0.0011 0.048 0.0025
Bud 0.0004 0.003 0.0012 0.0064 0.0002 0.0008 0.0006 0.0006 0.0004 0.0002 0.0042 0.0003 0.0004 0.0005 0.0006 0.0003 0.0005 0.0003
Bud Neck 0.0007 0.0003 0.0029 0.0029 0.0007 0.0031 0.0024 0.0002 0.0003 0.0002 0.0007 0.0011 0.0018 0.0002 0.0003 0.0004 0.0017 0.001
Bud Periphery 0.0004 0.0017 0.0009 0.0034 0.0002 0.001 0.0005 0.0003 0.0002 0.0001 0.0031 0.0003 0.0004 0.0002 0.0003 0.0001 0.0003 0.0002
Bud Site 0.0019 0.0189 0.0033 0.008 0.0006 0.0008 0.0117 0.0026 0.0009 0.0003 0.0018 0.0007 0.0043 0.0032 0.0008 0.0007 0.0005 0.0002
Cell Periphery 0.0009 0.001 0.0006 0.0002 0.0003 0.0004 0.0005 0.0002 0.0002 0.0002 0.0002 0.0001 0.0005 0.0003 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.6914 0.8977 0.8286 0.8008 0.6059 0.7906 0.7498 0.9159 0.8941 0.8485 0.6506 0.8365 0.7598 0.8801 0.8881 0.8245 0.8352 0.851
Cytoplasmic Foci 0.0497 0.0154 0.0323 0.0303 0.0675 0.0258 0.0305 0.0283 0.0222 0.0193 0.0587 0.0201 0.04 0.0225 0.0273 0.0314 0.0214 0.0229
Eisosomes 0.002 0 0.0005 0 0.0001 0.0002 0.0001 0 0 0 0 0 0.0001 0 0 0 0.0002 0
Endoplasmic Reticulum 0.0237 0.0086 0.0077 0.0088 0.0176 0.0228 0.0403 0.0033 0.0063 0.0064 0.0118 0.0188 0.0215 0.0056 0.008 0.0421 0.0055 0.0135
Endosome 0.0802 0.0222 0.0431 0.1003 0.1029 0.0528 0.0294 0.0182 0.0352 0.0363 0.1734 0.0182 0.0682 0.0283 0.0399 0.0426 0.056 0.0652
Golgi 0.0139 0.0016 0.012 0.0052 0.0138 0.0095 0.007 0.0077 0.0058 0.0021 0.0084 0.018 0.0145 0.0062 0.0076 0.0034 0.0146 0.0136
Lipid Particles 0.0095 0.0002 0.0026 0.0001 0.0069 0.0044 0.0028 0.0002 0.0044 0.0003 0.0004 0.0033 0.0119 0.0003 0.0049 0.006 0.0029 0.0069
Mitochondria 0.014 0.0004 0.0064 0.002 0.0434 0.0081 0.0039 0.0011 0.0079 0.0436 0.0098 0.0077 0.0043 0.0013 0.0016 0.0006 0.0034 0.0114
None 0.0744 0.0208 0.0199 0.0027 0.0135 0.0539 0.0259 0.0039 0.0099 0.0315 0.0652 0.056 0.0189 0.0452 0.0028 0.0096 0.0014 0.0027
Nuclear Periphery 0.002 0.0006 0.0008 0.0012 0.0005 0.0007 0.0359 0.0005 0.0006 0.0008 0.0009 0.0004 0.0045 0.0007 0.0009 0.0021 0.0011 0.0021
Nucleolus 0.0006 0.0001 0.0001 0.0002 0.0003 0.0001 0.0007 0 0 0 0.0001 0 0.0002 0 0 0.0001 0.0001 0.0001
Nucleus 0.0014 0.0008 0.0007 0.0018 0.0005 0.0007 0.0166 0.0004 0.0005 0.0007 0.0008 0.0003 0.0022 0.0005 0.0006 0.0008 0.0006 0.0007
Peroxisomes 0.0089 0.0001 0.0167 0.0003 0.0426 0.0015 0.0215 0.0024 0.0035 0.0004 0.0005 0.0092 0.0078 0.0002 0.0075 0.0261 0.0011 0.0009
Punctate Nuclear 0.0021 0.0003 0.0009 0.0003 0.0411 0.0003 0.0095 0.0002 0.0002 0.0002 0.0004 0.0005 0.0032 0.0002 0.0003 0.0017 0.0005 0.0006
Vacuole 0.0088 0.0041 0.0041 0.0199 0.0024 0.0033 0.0042 0.0021 0.002 0.0074 0.0058 0.0011 0.0063 0.0029 0.003 0.005 0.0032 0.0025
Vacuole Periphery 0.0014 0.0005 0.001 0.0044 0.0013 0.0011 0.0023 0.0005 0.0006 0.0011 0.0024 0.0004 0.0014 0.0005 0.0009 0.0016 0.0016 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.8918 11.8271 12.2867 16.1653 17.1536 17.1898 14.6489 24.2132 20.4332 13.6457
Translational Efficiency 1.9211 1.9023 2.0109 2.145 1.4119 1.47 1.8119 1.2836 1.5461 1.9611

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1411 1251 1702 1630 1506 1778 912 1484 2917 3029 2614 3114

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 858.22 1049.96 938.20 969.92 847.65 1016.73 935.24 1030.02 852.76 1030.45 937.17 998.56
Standard Deviation 103.33 139.91 114.37 129.56 107.85 146.34 128.04 143.36 105.82 144.64 119.33 139.57
Intensity Change Log 2 — 0.290915 0.128548 0.176518 — 0.262396 0.141868 0.281132 — 0.276814 0.135182 0.229449

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000307 0.001201 0.000276 0.001051 0.000383 0.000370 0.001481 0.003119 0.000346 0.000713 0.000696 0.002037
Bud Neck 0.004653 0.001954 0.004696 0.004960 0.002540 0.003374 0.002385 0.002523 0.003562 0.002788 0.003890 0.003798
Bud Site 0.026754 0.048348 0.012472 0.031091 0.022038 0.025297 0.021967 0.036529 0.024319 0.034817 0.015785 0.033683
Cell Periphery 0.000293 0.000276 0.000083 0.000106 0.000199 0.000181 0.000101 0.000110 0.000244 0.000220 0.000089 0.000108
Cytoplasm 0.449298* 0.557624* 0.560795* 0.507260* 0.507771* 0.533025* 0.635368* 0.657519* 0.479487* 0.543185* 0.586813* 0.578867*
Cytoplasmic Foci 0.331155* 0.281474* 0.101714 0.125234 0.298398* 0.289258* 0.167019 0.163773 0.314243* 0.286043* 0.124498 0.143600
Eisosomes 0.000119 0.000035 0.000036 0.000030 0.000078 0.000062 0.000031 0.000024 0.000098 0.000051 0.000034 0.000027
Endoplasmic Reticulum 0.009940 0.004246 0.003433 0.001011 0.010196 0.006476 0.001344 0.000409 0.010072 0.005555 0.002704 0.000724
Endosome 0.033022 0.020289 0.019975 0.028701 0.028121 0.027383 0.016847 0.011918 0.030492 0.024453 0.018884 0.020703
Golgi 0.042849 0.028854 0.000719 0.004116 0.034489 0.031690 0.002095 0.004506 0.038533 0.030519 0.001199 0.004302
Lipid Particles 0.004344 0.001413 0.004337 0.002400 0.003587 0.002980 0.003416 0.001974 0.003953 0.002333 0.004016 0.002197
Mitochondria 0.008091 0.001868 0.000697 0.001012 0.007483 0.005174 0.000158 0.000484 0.007777 0.003809 0.000509 0.000760
Mitotic Spindle 0.000340 0.002668 0.009374 0.014285 0.000289 0.001562 0.003909 0.005614 0.000314 0.002019 0.007467 0.010153
None 0.000582 0.000204 0.001853 0.001226 0.000694 0.001053 0.000520 0.000318 0.000640 0.000702 0.001388 0.000793
Nuclear Periphery 0.000043 0.000022 0.001502 0.000284 0.000072 0.000093 0.000165 0.000056 0.000058 0.000064 0.001035 0.000176
Nuclear Periphery Foci 0.000161 0.000258 0.002715 0.001202 0.000250 0.000302 0.005024 0.001299 0.000207 0.000284 0.003520 0.001248
Nucleolus 0.000112 0.000054 0.000723 0.000268 0.000203 0.000230 0.000170 0.000111 0.000159 0.000157 0.000530 0.000193
Nucleus 0.003491 0.004163 0.061289 0.011862 0.004398 0.004548 0.019743 0.008864 0.003960 0.004389 0.046794 0.010433
Peroxisomes 0.001580 0.001439 0.001674 0.003833 0.001279 0.002154 0.003201 0.005233 0.001425 0.001858 0.002207 0.004500
Vacuole 0.057367 0.032731 0.209678* 0.256762* 0.050431 0.050182 0.114277 0.094440 0.053786 0.042975 0.176393 0.179406
Vacuole Periphery 0.025499 0.010879 0.001959 0.003306 0.027102 0.014606 0.000780 0.001179 0.026327 0.013067 0.001548 0.002293

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.36 0.46 -2.59 0.87 -2.67 0.12 -2.27 -2.64 -2.67 -1.28 -2.98 -2.00 -3.27 -2.31 -2.36
Bud Neck 8.17 -0.43 0.27 -5.90 0.60 -2.05 0.47 0.44 3.06 -0.05 2.80 -1.25 0.18 -2.49 1.36
Bud Site -8.25 8.25 0.39 7.66 -6.22 -2.18 0.66 -5.27 -3.61 -4.65 -7.45 6.84 -3.32 3.26 -8.45
Cell Periphery 0.34 6.35 5.34 6.97 -0.73 1.07 7.49 7.45 6.53 0.04 1.10 8.95 7.54 8.82 -0.93
Cytoplasm -10.20 -10.23 -5.88 4.32 3.96 -2.64 -10.28 -14.42 -12.64 -2.14 -9.01 -12.93 -13.35 -4.62 0.09
Cytoplasmic Foci 6.12 38.37 34.04 23.81 -3.56 1.28 17.86 21.76 21.72 1.78 5.27 41.46 39.10 33.17 -2.64
Eisosomes 9.41 9.92 10.85 2.59 3.54 1.05 3.14 3.70 9.49 2.96 5.17 7.34 8.35 9.21 5.06
Endoplasmic Reticulum 3.90 5.33 7.36 4.39 6.92 2.52 7.84 8.74 6.33 6.63 4.28 8.80 11.34 7.59 8.53
Endosome 7.42 8.94 5.29 -1.49 -2.64 0.42 7.71 13.18 13.07 4.09 4.99 11.30 11.49 6.71 0.57
Golgi 7.63 30.15 25.75 18.84 -3.61 1.72 26.39 22.52 22.07 -2.27 6.54 40.32 34.10 29.10 -4.54
Lipid Particles 10.97 0.83 6.39 -3.23 4.08 2.17 0.83 7.20 4.27 4.11 8.06 0.71 9.20 1.34 5.63
Mitochondria 8.98 12.22 11.03 2.44 -0.43 2.29 11.79 10.90 5.84 -1.26 6.08 16.67 15.41 6.19 -0.65
Mitotic Spindle -2.35 -6.89 -5.89 -4.34 -1.21 -2.33 -4.65 -4.58 -3.13 -1.27 -3.28 -7.93 -7.29 -5.46 -1.07
None 1.68 -3.11 -0.64 -1.29 1.08 -0.90 1.18 2.38 1.84 2.50 -0.25 -2.88 -0.15 0.03 1.65
Nuclear Periphery 3.23 -9.03 -4.57 -4.94 7.04 -0.46 -7.03 -0.96 0.20 7.19 -0.24 -9.33 -4.61 -3.31 7.75
Nuclear Periphery Foci -2.46 -11.54 -5.89 -5.32 5.05 -0.55 -9.81 -11.02 -8.28 7.43 -1.32 -14.63 -10.39 -8.76 8.96
Nucleolus 6.99 -7.37 -6.65 -9.23 5.34 -0.47 0.48 1.58 1.44 3.42 -0.17 -6.38 -1.67 -0.74 5.98
Nucleus -0.68 -26.23 -12.92 -7.16 19.73 -0.35 -15.75 -10.99 -10.45 8.21 -0.88 -28.48 -16.75 -11.91 21.19
Peroxisomes 1.23 -0.34 -5.92 -6.18 -4.74 -2.30 -3.27 -6.59 -3.74 -2.13 -1.93 -3.02 -8.78 -5.57 -4.98
Vacuole 8.01 -27.09 -32.07 -36.73 -6.48 0.08 -14.51 -16.21 -16.84 1.17 5.01 -30.71 -34.12 -37.46 -2.32
Vacuole Periphery 9.24 18.05 16.96 8.55 -1.74 7.22 17.01 16.65 16.81 -0.94 11.29 24.30 23.52 17.88 -1.41
Standard name
Human Ortholog
Description Putative protein of unknown function; predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Ska1

Ska1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ska1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available