Standard name
Description Putative protein of unknown function; predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.94 0.82 0.77 0.72 0.57 0.58 0.89 0.96 0.97 0.94 0.88 0.94 0.91 0.87 0.8 0.84 0.82 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.09 0 0 0 0.05 0.06 0 0 0.08 0 0 0.09
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.14 0.32 0.24 0.48 0.52 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 1 0 1 1 1 1 0 1 0 0 0 0 0 0 2
Bud 0 0 1 0 1 2 1 2 0 1 0 0 0 0 1 0 1 3 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3 0 1 0 1 1 1 3 7 2 10 5 0 0 0 0 0 0 0
Cytoplasm 119 107 64 17 49 26 41 91 312 299 104 113 162 120 111 23 140 291 211
Endoplasmic Reticulum 2 2 1 0 1 0 3 9 8 3 3 6 10 4 4 2 4 10 27
Endosome 0 0 1 0 2 2 0 0 1 2 0 4 1 0 0 0 0 3 6
Golgi 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0 0 0 2
Mitochondria 2 3 11 7 16 22 37 2 2 1 6 5 6 2 2 1 3 8 9
Nucleus 0 1 0 0 1 0 2 1 0 1 0 0 1 0 1 0 1 1 1
Nuclear Periphery 0 2 1 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 0 0 0 1 2 0 0 0 0 0 0 2 12 13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 3 7 8
Vac/Vac Membrane 1 0 3 0 2 0 1 1 3 3 3 7 8 1 2 1 5 12 8
Unique Cell Count 122 114 78 22 68 46 71 102 325 309 111 129 173 133 128 29 167 357 304
Labelled Cell Count 127 116 84 25 74 55 87 110 335 316 126 143 189 133 128 29 167 357 304


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 6.6 6.5 5.3 5.8 5.1 5.1 7.6 7.9 7.4 7.3 8.7 9.3 8.6 8.9 8.7
Std Deviation (1e-4) 1.1 1.1 1.4 0.9 1.5 1.1 1.0 1.2 1.1 1.0 1.6 1.7 1.7 1.2 1.5 1.4
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ska1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available