Standard name | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | – | – | – | – | – | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud | 0 | 0 | – | – | – | – | – | 1 | 0 | 1 | 2 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 2 |
Bud Neck | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 |
Bud Site | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 0 | – | – | – | – | – | 1 | 0 | 1 | 1 | 1 | 3 | 7 | 2 | 10 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 119 | 107 | – | – | – | – | – | 64 | 17 | 49 | 26 | 41 | 91 | 312 | 299 | 104 | 113 | 162 | 120 | 111 | 23 | 140 | 291 | 211 |
Endoplasmic Reticulum | 2 | 2 | – | – | – | – | – | 1 | 0 | 1 | 0 | 3 | 9 | 8 | 3 | 3 | 6 | 10 | 4 | 4 | 2 | 4 | 10 | 27 |
Endosome | 0 | 0 | – | – | – | – | – | 1 | 0 | 2 | 2 | 0 | 0 | 1 | 2 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 3 | 6 |
Golgi | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 2 |
Mitochondria | 2 | 3 | – | – | – | – | – | 11 | 7 | 16 | 22 | 37 | 2 | 2 | 1 | 6 | 5 | 6 | 2 | 2 | 1 | 3 | 8 | 9 |
Nucleus | 0 | 1 | – | – | – | – | – | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 1 |
Nuclear Periphery | 0 | 2 | – | – | – | – | – | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | – | – | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 12 | 13 |
Peroxisomes | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | – | – | – | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 7 | 8 |
Vac/Vac Membrane | 1 | 0 | – | – | – | – | – | 3 | 0 | 2 | 0 | 1 | 1 | 3 | 3 | 3 | 7 | 8 | 1 | 2 | 1 | 5 | 12 | 8 |
Unique Cell Count | 122 | 114 | 78 | 22 | 68 | 46 | 71 | 102 | 325 | 309 | 111 | 129 | 173 | 133 | 128 | 29 | 167 | 357 | 304 | |||||
Labelled Cell Count | 127 | 116 | 84 | 25 | 74 | 55 | 87 | 110 | 335 | 316 | 126 | 143 | 189 | 133 | 128 | 29 | 167 | 357 | 304 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.7 | 6.6 | – | – | – | – | – | 6.5 | 5.3 | 5.8 | 5.1 | 5.1 | 7.6 | 7.9 | 7.4 | 7.3 | 8.7 | 9.3 | 8.6 | 8.9 | 8.7 |
Std Deviation (1e-4) | 1.1 | 1.1 | – | – | – | – | – | 1.4 | 0.9 | 1.5 | 1.1 | 1.0 | 1.2 | 1.1 | 1.0 | 1.6 | 1.7 | 1.7 | 1.2 | 1.5 | 1.4 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.7072 | 6.978 | 6.2858 | 5.5155 | 5.3676 | 6.4349 | 5.0994 | 6.1255 | 5.8254 | 5.8798 | 4.6659 | 5.6711 | 2.2045 | 3.2392 | 2.9454 | 2.9657 | 3.1042 | 2.881 |
Actin | 0.0117 | 0.0017 | 0.0137 | 0.0006 | 0.0376 | 0.018 | 0.0038 | 0.0113 | 0.0048 | 0.0004 | 0.0008 | 0.0068 | 0.0276 | 0.001 | 0.0046 | 0.0011 | 0.048 | 0.0025 |
Bud | 0.0004 | 0.003 | 0.0012 | 0.0064 | 0.0002 | 0.0008 | 0.0006 | 0.0006 | 0.0004 | 0.0002 | 0.0042 | 0.0003 | 0.0004 | 0.0005 | 0.0006 | 0.0003 | 0.0005 | 0.0003 |
Bud Neck | 0.0007 | 0.0003 | 0.0029 | 0.0029 | 0.0007 | 0.0031 | 0.0024 | 0.0002 | 0.0003 | 0.0002 | 0.0007 | 0.0011 | 0.0018 | 0.0002 | 0.0003 | 0.0004 | 0.0017 | 0.001 |
Bud Periphery | 0.0004 | 0.0017 | 0.0009 | 0.0034 | 0.0002 | 0.001 | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.0031 | 0.0003 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0002 |
Bud Site | 0.0019 | 0.0189 | 0.0033 | 0.008 | 0.0006 | 0.0008 | 0.0117 | 0.0026 | 0.0009 | 0.0003 | 0.0018 | 0.0007 | 0.0043 | 0.0032 | 0.0008 | 0.0007 | 0.0005 | 0.0002 |
Cell Periphery | 0.0009 | 0.001 | 0.0006 | 0.0002 | 0.0003 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.6914 | 0.8977 | 0.8286 | 0.8008 | 0.6059 | 0.7906 | 0.7498 | 0.9159 | 0.8941 | 0.8485 | 0.6506 | 0.8365 | 0.7598 | 0.8801 | 0.8881 | 0.8245 | 0.8352 | 0.851 |
Cytoplasmic Foci | 0.0497 | 0.0154 | 0.0323 | 0.0303 | 0.0675 | 0.0258 | 0.0305 | 0.0283 | 0.0222 | 0.0193 | 0.0587 | 0.0201 | 0.04 | 0.0225 | 0.0273 | 0.0314 | 0.0214 | 0.0229 |
Eisosomes | 0.002 | 0 | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0237 | 0.0086 | 0.0077 | 0.0088 | 0.0176 | 0.0228 | 0.0403 | 0.0033 | 0.0063 | 0.0064 | 0.0118 | 0.0188 | 0.0215 | 0.0056 | 0.008 | 0.0421 | 0.0055 | 0.0135 |
Endosome | 0.0802 | 0.0222 | 0.0431 | 0.1003 | 0.1029 | 0.0528 | 0.0294 | 0.0182 | 0.0352 | 0.0363 | 0.1734 | 0.0182 | 0.0682 | 0.0283 | 0.0399 | 0.0426 | 0.056 | 0.0652 |
Golgi | 0.0139 | 0.0016 | 0.012 | 0.0052 | 0.0138 | 0.0095 | 0.007 | 0.0077 | 0.0058 | 0.0021 | 0.0084 | 0.018 | 0.0145 | 0.0062 | 0.0076 | 0.0034 | 0.0146 | 0.0136 |
Lipid Particles | 0.0095 | 0.0002 | 0.0026 | 0.0001 | 0.0069 | 0.0044 | 0.0028 | 0.0002 | 0.0044 | 0.0003 | 0.0004 | 0.0033 | 0.0119 | 0.0003 | 0.0049 | 0.006 | 0.0029 | 0.0069 |
Mitochondria | 0.014 | 0.0004 | 0.0064 | 0.002 | 0.0434 | 0.0081 | 0.0039 | 0.0011 | 0.0079 | 0.0436 | 0.0098 | 0.0077 | 0.0043 | 0.0013 | 0.0016 | 0.0006 | 0.0034 | 0.0114 |
None | 0.0744 | 0.0208 | 0.0199 | 0.0027 | 0.0135 | 0.0539 | 0.0259 | 0.0039 | 0.0099 | 0.0315 | 0.0652 | 0.056 | 0.0189 | 0.0452 | 0.0028 | 0.0096 | 0.0014 | 0.0027 |
Nuclear Periphery | 0.002 | 0.0006 | 0.0008 | 0.0012 | 0.0005 | 0.0007 | 0.0359 | 0.0005 | 0.0006 | 0.0008 | 0.0009 | 0.0004 | 0.0045 | 0.0007 | 0.0009 | 0.0021 | 0.0011 | 0.0021 |
Nucleolus | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0007 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0014 | 0.0008 | 0.0007 | 0.0018 | 0.0005 | 0.0007 | 0.0166 | 0.0004 | 0.0005 | 0.0007 | 0.0008 | 0.0003 | 0.0022 | 0.0005 | 0.0006 | 0.0008 | 0.0006 | 0.0007 |
Peroxisomes | 0.0089 | 0.0001 | 0.0167 | 0.0003 | 0.0426 | 0.0015 | 0.0215 | 0.0024 | 0.0035 | 0.0004 | 0.0005 | 0.0092 | 0.0078 | 0.0002 | 0.0075 | 0.0261 | 0.0011 | 0.0009 |
Punctate Nuclear | 0.0021 | 0.0003 | 0.0009 | 0.0003 | 0.0411 | 0.0003 | 0.0095 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0005 | 0.0032 | 0.0002 | 0.0003 | 0.0017 | 0.0005 | 0.0006 |
Vacuole | 0.0088 | 0.0041 | 0.0041 | 0.0199 | 0.0024 | 0.0033 | 0.0042 | 0.0021 | 0.002 | 0.0074 | 0.0058 | 0.0011 | 0.0063 | 0.0029 | 0.003 | 0.005 | 0.0032 | 0.0025 |
Vacuole Periphery | 0.0014 | 0.0005 | 0.001 | 0.0044 | 0.0013 | 0.0011 | 0.0023 | 0.0005 | 0.0006 | 0.0011 | 0.0024 | 0.0004 | 0.0014 | 0.0005 | 0.0009 | 0.0016 | 0.0016 | 0.0017 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.8918 | 11.8271 | 12.2867 | 16.1653 | 17.1536 | 17.1898 | 14.6489 | 24.2132 | 20.4332 | 13.6457 |
Translational Efficiency | 1.9211 | 1.9023 | 2.0109 | 2.145 | 1.4119 | 1.47 | 1.8119 | 1.2836 | 1.5461 | 1.9611 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|