Standard name
Description Hexameric DNA polymerase alpha-associated DNA helicase A; involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.98 0.98 0.98 0.94 0.9 0.89 0.77 0.8 0.7 0.74 1.0 1.0 1.0 0.96 0.96 0.96 0.98 0.96 0.89 0.93 0.93 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.13 0.12 0.28 0.25 0.46 0.31 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 2 1 3 2 1
Bud 0 3 0 0 0 2 2 3 0 4 6 6 0 0 0 1 1 0 0 1 0 3 4 1
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 5 7 7 6 3 3 2 4 5 3 7 2 0 0 13 9 6 0 0 0 0 0 0
Cytoplasm 280 464 240 253 314 343 370 422 216 274 227 268 346 373 429 490 526 368 278 452 208 216 344 307
Endoplasmic Reticulum 0 1 1 2 0 2 0 0 2 0 0 1 0 0 1 23 30 14 0 0 1 0 2 1
Endosome 0 1 1 0 1 0 0 2 0 1 1 2 1 0 2 1 0 3 0 1 5 0 4 3
Golgi 1 1 1 2 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 2 1 0 0 2
Mitochondria 2 0 0 2 3 31 54 58 79 85 149 112 0 0 0 7 8 4 3 3 7 0 1 3
Nucleus 0 1 1 2 5 4 2 4 2 7 7 6 0 0 2 8 6 3 0 1 0 0 1 1
Nuclear Periphery 0 0 0 0 0 1 1 1 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 2 1 0 1 3 4 0 0 0 0 0 0 0 0 1 0 2 3
Vac/Vac Membrane 0 1 0 2 3 8 7 13 18 24 5 15 1 1 0 13 23 6 0 1 2 1 1 2
Unique Cell Count 281 470 245 258 320 364 409 476 280 342 323 363 347 374 431 510 547 382 284 470 233 233 372 336
Labelled Cell Count 283 477 251 270 332 394 443 507 323 403 402 422 350 374 434 557 603 405 284 470 233 233 372 336


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.7 6.6 6.4 6.4 5.6 5.4 5.7 4.7 5.0 4.3 4.6 6.8 6.8 6.6 6.8 7.2 6.5 6.4 6.8 6.8
Std Deviation (1e-4) 0.4 0.8 1.2 1.8 1.8 1.6 1.3 1.5 1.3 1.4 1.3 1.2 1.1 1.4 1.5 1.3 1.7 1.4 1.4 1.5 1.3
Intensity Change (Log2) – – – -0.06 -0.05 -0.25 -0.3 -0.22 -0.49 -0.42 -0.61 -0.54 0.03 0.04 -0.01 0.04 0.12 -0.03 -0.04 0.05 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.1 -0.8 0 0 0 0 -1.2 0 0 0 0 0 -0.2 0 -1.1
Cytoplasm 0.1 0.1 -2.2 -3.7 -4.3 -7.0 -6.5 -8.5 -7.9 2.1 2.2 1.9 -1.4 -1.3 -1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.0 3.4 2.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.7 5.9 5.7 0 8.4 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 4.0 4.2 0 3.2 0 0 0 0 3.3 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1556 0.6784 0.4658 0.3706 0.3802 0.5593 0.9022 1.0966 0.899 0.7339 0.7301 1.0425 0.9697 0.9788 0.8827 1.2947 1.2268 0.8468
Actin 0.0007 0.0052 0.0018 0.0027 0.0001 0.0045 0.0316 0.0041 0.0029 0.0226 0.0348 0.0063 0.0354 0.0023 0.0091 0.0014 0.0015 0.0027
Bud 0.0004 0.0111 0.0004 0.0016 0 0.0002 0.0035 0.0053 0.0121 0.0073 0.0112 0.0029 0.0005 0.0095 0.0009 0.0003 0.0015 0.0001
Bud Neck 0.0003 0.0002 0.0001 0.0001 0.0002 0.001 0.0072 0.0003 0.0004 0.0021 0.0133 0.0039 0.0011 0.0003 0.0029 0.0004 0.0003 0.0007
Bud Periphery 0.0003 0.0043 0.0007 0.0014 0 0.0003 0.0068 0.0014 0.0042 0.0059 0.0178 0.0017 0.0011 0.0014 0.0015 0.0001 0.0012 0.0002
Bud Site 0.0035 0.0119 0.0004 0.0001 0 0.0006 0.0183 0.0069 0.0105 0.0148 0.0135 0.0002 0.0012 0.008 0.0017 0.0043 0.0003 0.0001
Cell Periphery 0.0001 0.0003 0.0002 0.0001 0 0.0001 0.0005 0.0003 0.0003 0.0007 0.0005 0.0002 0.0003 0.0005 0.0002 0.0002 0 0
Cytoplasm 0.3451 0.4923 0.3714 0.4418 0.6407 0.5317 0.2101 0.3608 0.3096 0.2559 0.4432 0.3449 0.354 0.6699 0.5948 0.6677 0.5851 0.6629
Cytoplasmic Foci 0.0581 0.018 0.0111 0.0144 0.0034 0.011 0.0396 0.0121 0.0103 0.0237 0.0128 0.0123 0.0182 0.0078 0.0179 0.01 0.0076 0.0126
Eisosomes 0.0005 0.0001 0.0002 0.0001 0 0.0001 0.0004 0.0001 0.0001 0.0009 0.0001 0.0001 0.0004 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0031 0.0026 0.006 0.0009 0.0012 0.0029 0.0046 0.0012 0.0016 0.0023 0.0022 0.0019 0.0065 0.0013 0.0035 0.003 0.0014 0.0027
Endosome 0.0206 0.0025 0.0218 0.0149 0.0006 0.0122 0.0218 0.0013 0.0088 0.0078 0.0062 0.0076 0.015 0.0012 0.0087 0.0086 0.0032 0.0083
Golgi 0.003 0.0018 0.001 0.0437 0 0.006 0.0047 0.0002 0.0013 0.0024 0.0032 0.0012 0.0031 0.0002 0.0021 0.0011 0.0001 0.0006
Lipid Particles 0.014 0.0004 0.0017 0.0015 0 0.001 0.0101 0.0002 0.002 0.0028 0.0036 0.0017 0.0035 0.0001 0.0033 0.0021 0.0003 0.0015
Mitochondria 0.0018 0.0051 0.0043 0.001 0.0001 0.0147 0.012 0.0006 0.0126 0.0088 0.0012 0.0012 0.0015 0.0021 0.0012 0.0005 0.0018 0.0008
None 0.5194 0.4373 0.5566 0.4694 0.3508 0.4025 0.5812 0.5999 0.6075 0.6189 0.4131 0.6033 0.4933 0.2889 0.3293 0.247 0.3499 0.2887
Nuclear Periphery 0.002 0.001 0.0069 0.0006 0.0003 0.0008 0.0128 0.0004 0.001 0.0025 0.0021 0.0024 0.0211 0.0004 0.0027 0.0089 0.0065 0.0074
Nucleolus 0.0007 0.0003 0.0002 0.0001 0 0.0001 0.0028 0.0005 0.0008 0.0016 0.0006 0.0003 0.0007 0.001 0.0007 0.0008 0.0006 0.0002
Nucleus 0.0043 0.0016 0.0104 0.0016 0.0017 0.0014 0.0086 0.0016 0.0034 0.0045 0.0029 0.0025 0.0259 0.0023 0.0044 0.0256 0.0338 0.0038
Peroxisomes 0.0039 0.0009 0.0004 0.0011 0 0.0055 0.008 0.0003 0.0033 0.0023 0.0093 0.0006 0.0066 0.0006 0.001 0.0004 0.0002 0.0011
Punctate Nuclear 0.0086 0.0018 0.0013 0.0018 0.0005 0.0007 0.0088 0.001 0.002 0.0084 0.0054 0.0023 0.0081 0.0009 0.0115 0.0135 0.0022 0.0042
Vacuole 0.0079 0.001 0.002 0.0007 0.0003 0.0022 0.0045 0.0014 0.0047 0.003 0.0026 0.002 0.0016 0.0012 0.0017 0.0033 0.0019 0.0009
Vacuole Periphery 0.0015 0.0002 0.001 0.0002 0 0.0006 0.002 0.0002 0.0005 0.0008 0.0003 0.0004 0.0009 0.0002 0.0005 0.0007 0.0005 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.9904 9.6138 5.3504 8.889 7.3204 9.1362 7.6549 7.8728 8.4759 10.5775
Translational Efficiency 0.9697 0.5495 0.8346 0.6644 0.7115 0.611 0.8932 1.2357 0.9142 0.7478

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘