Standard name
Human Ortholog
Description Transcriptional activator; binds specific gene recruitment sequences and is required for DNA zip code-mediated targeting of genes to nuclear periphery; regulates proline utilization genes, constitutively binds PUT1 and PUT2 promoters as a dimer, undergoes conformational change to form active state; binds other promoters only under activating conditions; differentially phosphorylated in presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0 0.07 0.07 0.11 0.09 – 0.14 0.11 0.1 0.18 0.19 0.08 0.1 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.08 0.14 0.28 0.08 0.52 0.53 0.59 0.83 – 0.8 0.81 0 0 0.11 0 0 0 0 0 0 0 0 0
Nucleus 0.95 0.91 0.92 0.85 0.87 0.77 0.7 0.59 0.49 – 0.37 0.39 0.88 0.78 0.75 0.83 0.83 0.84 0.87 0.87 0.88 0.88 0.75 0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0.05 0 0 0 0 – 0 0.05 0 0.05 0 0.08 0.06 0.05 0 0.05 0.08 0.06 0.15 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 4
Bud 0 0 0 0 0 1 0 6 2 – 1 4 0 0 1 0 1 0 2 0 1 2 2 2
Bud Neck 0 0 0 0 0 0 0 3 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 1 1 2 5 7 5 – 2 10 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 9 5 11 7 14 20 33 20 – 26 18 29 70 77 13 13 11 0 2 0 0 3 2
Endoplasmic Reticulum 0 2 0 0 0 2 0 0 2 – 1 0 0 0 1 2 3 3 0 0 0 0 1 2
Endosome 0 0 1 0 2 0 0 0 0 – 0 0 0 1 0 2 3 2 2 2 0 0 1 6
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 1 1 0 0 1 4
Mitochondria 2 20 24 42 16 102 144 183 176 – 144 136 7 3 44 5 2 3 0 6 1 0 2 8
Nucleus 104 236 159 127 165 152 190 181 103 – 66 65 258 305 304 129 110 117 103 228 152 164 235 155
Nuclear Periphery 0 1 0 1 0 0 1 1 1 – 0 0 0 0 2 0 1 0 1 1 1 0 0 2
Nucleolus 2 2 1 2 5 1 3 1 1 – 0 3 0 2 4 0 2 3 0 0 0 2 8 14
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 1 0 0 1 0 1 0 0 – 0 0 0 2 0 1 3 0 0 1 0 0 3 4
Vac/Vac Membrane 5 7 2 1 9 5 7 10 5 – 6 8 3 21 13 13 8 7 4 13 13 11 48 46
Unique Cell Count 110 258 173 149 189 198 271 308 212 180 168 293 393 408 155 132 139 118 263 174 186 314 260
Labelled Cell Count 114 278 192 185 206 279 371 425 315 246 244 298 405 446 166 146 146 118 263 174 186 314 260


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 5.3 4.6 4.5 4.8 3.8 3.9 3.7 3.0 – 3.1 3.1 5.4 5.4 4.6 8.0 8.8 9.0 5.7 5.7 6.6
Std Deviation (1e-4) 1.0 0.9 0.8 1.3 1.0 0.9 1.6 1.7 0.9 – 1.2 1.6 1.0 1.2 1.1 1.6 2.2 1.9 1.3 1.2 1.7
Intensity Change (Log2) – – – -0.02 0.06 -0.28 -0.25 -0.3 -0.59 – -0.56 -0.58 0.23 0.23 0.01 0.81 0.94 0.98 0.32 0.31 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 1.8 0.4 1.8 2.0 3.1 2.6 – 3.8 2.9 2.8 4.8 5.1 2.2 2.6 2.0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 -4.8 -6.7 0 -3.4 -3.8 -3.7
Nucleus -1.9 -1.4 -4.0 -5.5 -7.7 -9.1 – -10.8 -10.3 -1.3 -4.1 -4.8 -2.4 -2.3 -2.1
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 2.0 0 0 0 0 – 0 0 0 2.3 0 3.1 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.5987 3.3757 3.7876 2.9168 3.4768 3.0259 4.6309 4.5767 4.1642 4.7013 4.5216 4.385 4.4395 4.2674 4.2277 4.0899 4.2314 4.236
Actin 0.0027 0 0.0031 0 0.0001 0.0002 0.0194 0 0.013 0.0009 0.0021 0.0034 0.0224 0.0001 0.0002 0.0021 0.001 0
Bud 0.0002 0 0.0022 0 0 0 0.0005 0 0.0007 0.0002 0.0004 0.0007 0.0008 0.0017 0.0001 0.0001 0.0003 0
Bud Neck 0.0027 0.0002 0.0006 0.0001 0.0002 0.0014 0.0013 0.0001 0.0011 0.0005 0.0012 0.0023 0.0032 0.0001 0.0003 0.0002 0.0006 0.0004
Bud Periphery 0.0004 0 0.0023 0 0 0.0001 0.0009 0 0.0007 0.0001 0.0007 0.0016 0.0017 0.0002 0.0001 0.0001 0.0007 0
Bud Site 0.0002 0.0004 0.002 0 0 0.0001 0.0023 0.0001 0.0161 0.0014 0.0055 0.0009 0.0022 0.0006 0.0001 0.0001 0.0005 0
Cell Periphery 0.0001 0 0.0001 0 0 0 0.0002 0 0.0002 0 0.0002 0.0002 0.0002 0 0 0 0.0001 0
Cytoplasm 0.0138 0.0039 0.0219 0.0001 0.0047 0.0002 0.0142 0.0042 0.0037 0.003 0.0039 0.0008 0.0026 0.0066 0.0012 0.0006 0.0032 0.0001
Cytoplasmic Foci 0.0004 0.0001 0.0003 0 0 0 0.0343 0.0002 0.0136 0.011 0.0131 0.0044 0.0076 0.0002 0.0001 0.0151 0.001 0
Eisosomes 0 0 0 0 0 0 0.0002 0 0.0001 0 0 0.0001 0.0004 0 0 0 0 0
Endoplasmic Reticulum 0.0034 0.0004 0.0009 0 0.0004 0.0001 0.0086 0.0001 0.0006 0.0009 0.001 0.0006 0.0043 0.0006 0.0004 0.0005 0.0024 0
Endosome 0.0059 0.0012 0.0012 0 0.0003 0.0001 0.0393 0.0003 0.009 0.0268 0.0165 0.0031 0.0099 0.0002 0.0009 0.0141 0.0085 0
Golgi 0.0005 0 0.0002 0 0 0 0.0054 0 0.0091 0.01 0.0069 0.0016 0.0034 0 0 0.0101 0.0003 0
Lipid Particles 0.0003 0.0001 0.0001 0 0 0 0.0119 0 0.0054 0.0087 0.015 0.0012 0.0018 0 0.0001 0.0146 0.0012 0
Mitochondria 0.0015 0.0002 0.0015 0.0001 0.0006 0.0001 0.0031 0.0001 0.005 0.0021 0.0027 0.0056 0.0066 0.0012 0.0003 0.001 0.0063 0
None 0.001 0.0036 0.0005 0 0.0007 0.0001 0.0068 0.0005 0.0011 0.0009 0.0012 0.0002 0.004 0.0013 0.0002 0.0001 0.0017 0
Nuclear Periphery 0.0585 0.0077 0.0131 0.0011 0.0105 0.002 0.0485 0.0034 0.0062 0.0051 0.0069 0.0054 0.0093 0.0072 0.0026 0.0019 0.0141 0.0003
Nucleolus 0.0044 0.012 0.0053 0.0052 0.008 0.0191 0.0102 0.0067 0.0077 0.0062 0.0135 0.0125 0.0085 0.0085 0.0078 0.0087 0.0126 0.0117
Nucleus 0.8966 0.9527 0.9297 0.9928 0.9692 0.9742 0.736 0.9715 0.8919 0.9085 0.8909 0.949 0.9018 0.9656 0.9711 0.9252 0.9363 0.9746
Peroxisomes 0.0001 0 0.0001 0 0 0 0.0067 0 0.0064 0.0037 0.0068 0.0021 0.001 0 0 0.0044 0.0008 0
Punctate Nuclear 0.0031 0.014 0.0135 0.0005 0.0045 0.0018 0.0381 0.0119 0.0073 0.0076 0.0084 0.0027 0.0054 0.0052 0.0138 0.0007 0.0029 0.0127
Vacuole 0.0022 0.0027 0.0011 0 0.0003 0.0003 0.0088 0.0008 0.0009 0.0017 0.0024 0.0012 0.0016 0.0006 0.0005 0.0002 0.0034 0.0001
Vacuole Periphery 0.0021 0.0007 0.0005 0 0.0003 0.0001 0.0032 0.0001 0.0003 0.0005 0.0007 0.0005 0.0015 0.0002 0.0001 0.0002 0.0019 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.5135 18.4526 20.9124 20.9221 14.8414 14.6289 24.8483 24.8416 17.2098 19.7138
Translational Efficiency 0.325 0.3353 0.2065 0.1954 0.2467 0.3071 0.2452 0.2059 0.319 0.2046

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2307 904 956 668 2330 1450 1292 210 4637 2354 2248 878

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 705.75 841.22 927.24 863.67 721.20 779.04 877.41 942.15 713.51 802.92 898.60 882.44
Standard Deviation 77.28 111.78 106.95 125.65 95.20 132.30 121.94 131.31 87.09 128.43 118.39 131.37
Intensity Change Log 2 — 0.253326 0.393786 0.291323 — 0.111298 0.282852 0.385557 — 0.183291 0.338784 0.339719

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000043 0.000209 0.000553 0.000604 0.000075 0.000362 0.000381 0.001287 0.000059 0.000303 0.000454 0.000767
Bud Neck 0.001580 0.003902 0.010747 0.008090 0.002491 0.003718 0.005551 0.018397 0.002038 0.003789 0.007761 0.010556
Bud Site 0.000782 0.001451 0.002213 0.005574 0.000312 0.001735 0.001006 0.022852 0.000546 0.001626 0.001520 0.009707
Cell Periphery 0.000078 0.000071 0.000066 0.000232 0.000132 0.000975 0.000199 0.000414 0.000105 0.000628 0.000142 0.000275
Cytoplasm 0.022282 0.004551 0.002021 0.063583 0.017516 0.011754 0.015766 0.019999 0.019887 0.008988 0.009921 0.053159
Cytoplasmic Foci 0.001427 0.000977 0.000049 0.004030 0.000959 0.001787 0.001207 0.005330 0.001192 0.001476 0.000715 0.004341
Eisosomes 0.000007 0.000014 0.000021 0.000035 0.000009 0.002805 0.000033 0.000053 0.000008 0.001733 0.000028 0.000039
Endoplasmic Reticulum 0.001099 0.003685 0.005609 0.004963 0.001847 0.003410 0.004142 0.007370 0.001475 0.003515 0.004766 0.005539
Endosome 0.000194 0.000411 0.000259 0.007020 0.000191 0.000523 0.000405 0.024435 0.000193 0.000480 0.000343 0.011185
Golgi 0.000100 0.000180 0.000107 0.005744 0.000059 0.000471 0.000079 0.001843 0.000079 0.000359 0.000091 0.004811
Lipid Particles 0.000480 0.000419 0.000104 0.000766 0.000311 0.001574 0.000245 0.000735 0.000395 0.001131 0.000185 0.000759
Mitochondria 0.001719 0.001658 0.000968 0.005343 0.000952 0.002047 0.001049 0.005140 0.001333 0.001898 0.001015 0.005294
Mitotic Spindle 0.000830 0.003305 0.001512 0.018788 0.001160 0.003487 0.001832 0.030553 0.000996 0.003417 0.001696 0.021602
None 0.006711 0.004551 0.001448 0.019518 0.008675 0.018032 0.013941 0.012991 0.007698 0.012855 0.008628 0.017956
Nuclear Periphery 0.000636 0.000831 0.000539 0.001532 0.000832 0.001291 0.000524 0.001128 0.000735 0.001114 0.000531 0.001436
Nuclear Periphery Foci 0.000178 0.000327 0.000091 0.001527 0.000174 0.000755 0.000099 0.000533 0.000176 0.000590 0.000096 0.001289
Nucleolus 0.003019 0.005033 0.002660 0.003358 0.004618 0.003143 0.003225 0.001611 0.003822 0.003869 0.002984 0.002940
Nucleus 0.957790* 0.966791* 0.969079* 0.828655* 0.958913* 0.935406* 0.947644* 0.821294* 0.958354* 0.947459* 0.956759* 0.826895*
Peroxisomes 0.000431 0.000497 0.000116 0.000762 0.000217 0.004526 0.000103 0.001953 0.000324 0.002979 0.000108 0.001047
Vacuole 0.000501 0.000942 0.001671 0.016434 0.000463 0.001985 0.002059 0.020688 0.000482 0.001584 0.001894 0.017452
Vacuole Periphery 0.000112 0.000194 0.000168 0.003440 0.000095 0.000214 0.000511 0.001395 0.000104 0.000206 0.000365 0.002951

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.00 -15.58 -11.58 -9.29 -5.04 -1.56 -13.29 -10.12 -5.03 -7.03 -2.13 -20.35 -14.72 -6.53 -8.12
Bud Neck -4.19 -8.11 -9.30 -7.03 -0.72 -2.49 -8.06 -6.94 -6.27 -4.55 -4.70 -11.25 -11.32 -9.34 -3.98
Bud Site -0.83 -1.83 -5.15 -3.87 -3.06 -2.25 -3.98 -2.57 -2.19 -2.30 -2.13 -3.10 -5.39 -4.25 -4.25
Cell Periphery 0.48 0.97 -4.31 -4.22 -4.58 -3.89 -1.62 -1.24 3.44 -0.43 -3.91 -1.55 -3.35 3.26 -2.11
Cytoplasm 8.59 11.82 -5.44 -9.68 -10.68 2.82 1.84 0.58 -1.36 -0.70 7.19 7.90 -5.67 -8.81 -9.02
Cytoplasmic Foci 0.90 4.82 -1.79 -2.34 -4.65 -1.58 0.18 -0.93 0.10 -0.98 -0.75 3.10 -2.35 -1.57 -3.86
Eisosomes -5.27 -14.18 -12.19 -9.96 -7.73 -3.82 -11.78 -8.41 3.74 -4.35 -3.82 -16.17 -14.56 3.71 -7.46
Endoplasmic Reticulum -10.92 -17.97 -13.22 -8.02 -4.18 -4.93 -9.85 -8.93 -7.36 -5.64 -9.63 -17.75 -15.48 -10.77 -6.82
Endosome -2.08 -1.43 -3.36 -3.13 -3.28 -2.29 -2.73 -1.77 -1.68 -1.71 -2.93 -3.03 -3.54 -3.28 -3.40
Golgi -1.00 0.00 -1.61 -1.56 -1.61 -3.20 -0.54 -1.98 -0.37 -1.91 -3.26 -0.21 -1.73 -1.48 -1.73
Lipid Particles 0.30 2.12 -1.67 -2.31 -5.05 -3.31 0.58 -3.18 1.62 -3.64 -2.88 2.05 -3.13 0.94 -5.54
Mitochondria 0.04 1.24 -1.55 -1.50 -2.28 -1.55 -0.51 -2.70 -0.81 -2.51 -1.03 0.79 -2.29 -1.53 -2.66
Mitotic Spindle -1.78 -0.65 -5.05 -4.14 -4.79 -1.69 -1.46 -3.35 -3.16 -3.23 -2.48 -1.63 -5.89 -5.20 -5.58
None 1.54 6.83 -2.78 -3.47 -5.57 -3.10 -1.47 0.34 2.99 1.45 -2.65 0.37 -2.30 0.48 -2.29
Nuclear Periphery -1.28 0.17 -8.89 -6.86 -9.57 -0.91 0.92 -5.73 -2.58 -6.21 -1.25 0.67 -10.37 -4.50 -11.18
Nuclear Periphery Foci -1.10 0.81 -2.50 -2.21 -2.60 -1.63 0.66 -2.04 -0.82 -2.09 -1.83 0.99 -3.00 -1.85 -3.08
Nucleolus -2.02 0.75 -1.58 1.04 -2.05 2.15 2.23 3.23 1.04 1.46 -0.02 2.20 0.52 0.44 -1.27
Nucleus -1.66 -2.26 12.55 12.77 13.43 3.98 3.01 7.29 5.85 6.58 2.81 1.27 14.65 12.42 14.04
Peroxisomes -0.39 1.41 -0.26 0.19 -2.74 -3.31 1.61 -1.16 2.86 -1.52 -3.29 1.90 -1.17 3.02 -3.12
Vacuole -2.54 -6.86 -8.28 -8.09 -7.77 -3.42 -5.44 -5.14 -4.96 -4.86 -4.06 -7.56 -9.60 -9.20 -8.93
Vacuole Periphery -1.58 -0.73 -2.43 -2.28 -2.34 -2.40 -1.00 -1.72 -1.18 -0.20 -2.79 -1.10 -2.63 -2.39 -2.04