Standard name | |
---|---|
Human Ortholog | |
Description | Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 |
Bud | 0 | 0 | 6 | 6 | 3 | 0 | 3 | 5 | 5 | 9 | 8 | 4 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 15 | 18 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 3 | 3 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 68 | 110 | 111 | 54 | 126 | 144 | 208 | 230 | 129 | 156 | 76 | 76 | 47 | 80 | 97 | 82 | 92 | 107 | 93 | 83 | 124 | 80 | 115 | 122 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 1 | 2 | 0 | 1 | 0 | 3 | 8 | 11 | 0 | 0 | 0 | 0 | 2 | 6 |
Endosome | 0 | 0 | 0 | 6 | 13 | 0 | 9 | 8 | 0 | 1 | 0 | 0 | 2 | 2 | 2 | 3 | 2 | 2 | 0 | 1 | 1 | 2 | 10 | 12 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 10 | 0 | 0 | 1 | 0 | 0 | 1 |
Mitochondria | 0 | 2 | 11 | 48 | 35 | 57 | 140 | 153 | 156 | 201 | 122 | 135 | 1 | 1 | 5 | 3 | 2 | 5 | 0 | 1 | 0 | 0 | 0 | 2 |
Nucleus | 6 | 4 | 2 | 19 | 31 | 15 | 35 | 62 | 35 | 44 | 25 | 28 | 22 | 54 | 67 | 13 | 25 | 18 | 22 | 28 | 11 | 37 | 118 | 103 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 8 | 11 |
Vac/Vac Membrane | 34 | 22 | 29 | 80 | 151 | 57 | 155 | 156 | 70 | 72 | 35 | 45 | 92 | 136 | 128 | 66 | 75 | 106 | 1 | 6 | 5 | 7 | 39 | 59 |
Unique Cell Count | 88 | 122 | 141 | 173 | 295 | 229 | 443 | 492 | 305 | 387 | 208 | 220 | 133 | 242 | 275 | 127 | 160 | 197 | 121 | 126 | 155 | 135 | 321 | 354 |
Labelled Cell Count | 108 | 138 | 160 | 214 | 359 | 276 | 553 | 620 | 396 | 490 | 270 | 294 | 164 | 275 | 302 | 173 | 211 | 259 | 121 | 126 | 155 | 135 | 321 | 354 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.5 | 6.5 | 5.9 | 5.4 | 5.8 | 5.4 | 5.3 | 5.4 | 4.8 | 4.7 | 4.7 | 4.4 | 8.5 | 8.6 | 7.6 | 11.5 | 12.8 | 13.1 | 8.5 | 8.3 | 9.2 |
Std Deviation (1e-4) | 1.2 | 1.2 | 1.1 | 0.8 | 1.3 | 1.1 | 1.3 | 1.4 | 1.2 | 1.1 | 1.2 | 1.5 | 1.3 | 1.5 | 1.3 | 2.2 | 2.8 | 2.9 | 1.3 | 1.5 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.05 | -0.13 | -0.18 | -0.14 | -0.32 | -0.34 | -0.35 | -0.43 | 0.52 | 0.54 | 0.35 | 0.95 | 1.1 | 1.14 | 0.52 | 0.48 | 0.63 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -0.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -8.4 | -7.1 | -3.2 | -6.6 | -6.7 | -7.2 | -7.8 | -7.8 | -8.2 | -7.3 | -8.6 | -8.4 | -2.6 | -3.9 | -4.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.5 | 1.3 | 4.1 | 5.6 | 5.6 | 0 | 0 | 0 | 0 | -2.8 | 0 | -3.0 | -2.0 | -2.8 | -2.2 |
Nucleus | 3.4 | 3.4 | 2.3 | 2.8 | 3.9 | 3.6 | 3.6 | 3.6 | 3.8 | 4.4 | 5.6 | 6.0 | 3.1 | 4.3 | 3.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 4.8 | 6.1 | 1.0 | 3.2 | 2.6 | 0.6 | -0.5 | -0.9 | 0 | 8.1 | 6.8 | 5.2 | 5.4 | 4.8 | 6.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.5926 | 5.1785 | 4.43 | 3.8715 | 3.5201 | 4.7463 | 4.7454 | 7.1734 | 6.4647 | 6.6788 | 5.8377 | 6.6439 | 4.2122 | 5.9172 | 4.9296 | 4.8582 | 4.9453 | 4.9224 |
Actin | 0.0256 | 0.0004 | 0.0112 | 0.045 | 0.0014 | 0.0003 | 0.0515 | 0.0003 | 0.0187 | 0.0243 | 0.0297 | 0.0021 | 0.0047 | 0.0005 | 0.0035 | 0.0005 | 0.0181 | 0.0021 |
Bud | 0.0013 | 0.0004 | 0.0004 | 0.0008 | 0.0004 | 0.0002 | 0.0007 | 0.0005 | 0.0015 | 0.0033 | 0.0004 | 0.0003 | 0.001 | 0.0002 | 0.0047 | 0.0002 | 0.0002 | 0.0003 |
Bud Neck | 0.0034 | 0.0013 | 0.0046 | 0.0251 | 0.0472 | 0.0023 | 0.0034 | 0.0004 | 0.002 | 0.0035 | 0.0064 | 0.0021 | 0.0134 | 0.0007 | 0.001 | 0.0013 | 0.001 | 0.0034 |
Bud Periphery | 0.0017 | 0.0003 | 0.0004 | 0.0009 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0005 | 0.0046 | 0.0006 | 0.0002 | 0.0005 | 0 | 0.0015 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.0029 | 0.0146 | 0.0059 | 0.0038 | 0.0009 | 0.0002 | 0.0044 | 0.0014 | 0.002 | 0.0063 | 0.0009 | 0.0003 | 0.0051 | 0.0009 | 0.0019 | 0.0003 | 0.0005 | 0.0002 |
Cell Periphery | 0.0003 | 0.0003 | 0.0003 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.2578 | 0.3779 | 0.465 | 0.3793 | 0.6019 | 0.2734 | 0.2449 | 0.4221 | 0.3391 | 0.534 | 0.5016 | 0.2073 | 0.3124 | 0.4029 | 0.4563 | 0.5566 | 0.6133 | 0.2215 |
Cytoplasmic Foci | 0.0105 | 0.0009 | 0.013 | 0.0106 | 0.0031 | 0.0009 | 0.0238 | 0.0041 | 0.0101 | 0.0043 | 0.02 | 0.001 | 0.0237 | 0.0015 | 0.0047 | 0.0017 | 0.0037 | 0.0021 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0005 | 0 | 0 | 0.0009 | 0 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0108 | 0.003 | 0.0042 | 0.0017 | 0.0071 | 0.0028 | 0.0083 | 0.0022 | 0.0021 | 0.002 | 0.0036 | 0.0017 | 0.0048 | 0.0052 | 0.0033 | 0.0059 | 0.0034 | 0.0025 |
Endosome | 0.0209 | 0.001 | 0.0049 | 0.0078 | 0.0204 | 0.003 | 0.0389 | 0.0008 | 0.0127 | 0.0105 | 0.0215 | 0.0013 | 0.0078 | 0.0025 | 0.0034 | 0.0018 | 0.0038 | 0.0032 |
Golgi | 0.0061 | 0.0001 | 0.0034 | 0.0109 | 0.0185 | 0.0006 | 0.0159 | 0 | 0.0068 | 0.0023 | 0.0074 | 0.0003 | 0.0016 | 0.0001 | 0.0019 | 0.0001 | 0.0028 | 0.0002 |
Lipid Particles | 0.0053 | 0.0001 | 0.0095 | 0.0209 | 0.0012 | 0.0018 | 0.0175 | 0.0001 | 0.0026 | 0.0003 | 0.0228 | 0 | 0.0108 | 0.0002 | 0.0011 | 0.0001 | 0.0025 | 0.0001 |
Mitochondria | 0.0151 | 0.0004 | 0.0023 | 0.0288 | 0.0098 | 0.0157 | 0.0111 | 0.0006 | 0.0038 | 0.0037 | 0.022 | 0.0054 | 0.0009 | 0.0004 | 0.0038 | 0.0004 | 0.0009 | 0.0089 |
None | 0.0049 | 0.0096 | 0.0035 | 0.002 | 0.0014 | 0.0026 | 0.01 | 0.0027 | 0.0016 | 0.0031 | 0.0096 | 0.0006 | 0.0353 | 0.0213 | 0.0135 | 0.0154 | 0.0564 | 0.0021 |
Nuclear Periphery | 0.0382 | 0.0147 | 0.0169 | 0.0117 | 0.0127 | 0.0144 | 0.0305 | 0.0116 | 0.0102 | 0.0088 | 0.0149 | 0.0135 | 0.0126 | 0.0166 | 0.0142 | 0.01 | 0.0104 | 0.0131 |
Nucleolus | 0.0019 | 0.0011 | 0.0011 | 0.0024 | 0.0007 | 0.0006 | 0.0012 | 0.0013 | 0.0004 | 0.0003 | 0.0007 | 0.0005 | 0.0019 | 0.0005 | 0.0009 | 0.0004 | 0.0002 | 0.0008 |
Nucleus | 0.5711 | 0.5671 | 0.4292 | 0.4173 | 0.2659 | 0.6742 | 0.5082 | 0.5465 | 0.5766 | 0.3755 | 0.306 | 0.7547 | 0.5336 | 0.5391 | 0.4639 | 0.3992 | 0.2751 | 0.7294 |
Peroxisomes | 0.0052 | 0 | 0.0097 | 0.0183 | 0.0004 | 0.0004 | 0.0072 | 0.0001 | 0.0009 | 0.001 | 0.0149 | 0.0004 | 0.008 | 0.0001 | 0.0012 | 0.0001 | 0.0019 | 0.0007 |
Punctate Nuclear | 0.0048 | 0.004 | 0.0112 | 0.0073 | 0.0025 | 0.0016 | 0.0163 | 0.0041 | 0.0065 | 0.006 | 0.0134 | 0.0068 | 0.0188 | 0.0056 | 0.0176 | 0.0048 | 0.0042 | 0.0075 |
Vacuole | 0.0092 | 0.0029 | 0.0027 | 0.0034 | 0.0031 | 0.004 | 0.0026 | 0.0007 | 0.0012 | 0.0041 | 0.002 | 0.0011 | 0.0024 | 0.0017 | 0.0011 | 0.001 | 0.0011 | 0.0015 |
Vacuole Periphery | 0.0029 | 0.0003 | 0.0004 | 0.0011 | 0.001 | 0.0008 | 0.0017 | 0.0002 | 0.0005 | 0.0013 | 0.0013 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 26.3955 | 24.1498 | 27.6201 | 34.5912 | 26.0287 | 28.2387 | 27.8901 | 32.0536 | 34.2922 | 30.5171 |
Translational Efficiency | 0.8325 | 0.7485 | 0.7298 | 0.7001 | 0.6091 | 0.7886 | 0.753 | 0.5383 | 0.6236 | 0.5712 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
174 | 1502 | 120 | 1639 | 1322 | 1963 | 1508 | 2024 | 1496 | 3465 | 1628 | 3663 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1013.81 | 923.06 | 1396.66 | 1129.03 | 869.10 | 918.45 | 974.30 | 1034.53 | 885.93 | 920.45 | 1005.43 | 1076.81 |
Standard Deviation | 195.25 | 143.21 | 184.41 | 171.47 | 119.01 | 145.85 | 167.23 | 142.09 | 138.21 | 144.73 | 201.47 | 162.85 |
Intensity Change Log 2 | — | -0.135291 | 0.462194 | 0.155297 | — | 0.079679 | 0.164844 | 0.251381 | — | -0.032075 | 0.332507 | 0.200443 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001597 | 0.000203 | 0.002224 | 0.000544 | 0.000217 | 0.000184 | 0.000211 | 0.000551 | 0.000378 | 0.000193 | 0.000360 | 0.000548 |
Bud Neck | 0.029095 | 0.001940 | 0.008202 | 0.008850 | 0.001643 | 0.004041 | 0.003356 | 0.012108 | 0.004836 | 0.003130 | 0.003713 | 0.010650 |
Bud Site | 0.036803 | 0.002613 | 0.003964 | 0.003083 | 0.001677 | 0.004949 | 0.001592 | 0.006861 | 0.005763 | 0.003936 | 0.001767 | 0.005170 |
Cell Periphery | 0.000412 | 0.000075 | 0.000315 | 0.000146 | 0.000093 | 0.000060 | 0.000852 | 0.000135 | 0.000130 | 0.000066 | 0.000812 | 0.000140 |
Cytoplasm | 0.188562* | 0.574825* | 0.050151 | 0.072431 | 0.430997* | 0.605013* | 0.185491* | 0.100883* | 0.402800* | 0.591927* | 0.175515* | 0.088152 |
Cytoplasmic Foci | 0.024109 | 0.016789 | 0.001372 | 0.000946 | 0.015688 | 0.021417 | 0.007097 | 0.000717 | 0.016668 | 0.019411 | 0.006675 | 0.000820 |
Eisosomes | 0.000067 | 0.000013 | 0.000057 | 0.000017 | 0.000017 | 0.000013 | 0.000390 | 0.000018 | 0.000023 | 0.000013 | 0.000365 | 0.000017 |
Endoplasmic Reticulum | 0.021482 | 0.001728 | 0.013990 | 0.004571 | 0.003222 | 0.001444 | 0.002289 | 0.005211 | 0.005346 | 0.001567 | 0.003151 | 0.004925 |
Endosome | 0.004259 | 0.002477 | 0.002057 | 0.001873 | 0.000929 | 0.002281 | 0.000812 | 0.000921 | 0.001317 | 0.002366 | 0.000903 | 0.001347 |
Golgi | 0.000257 | 0.000671 | 0.000241 | 0.000252 | 0.000094 | 0.001176 | 0.000060 | 0.000091 | 0.000113 | 0.000957 | 0.000073 | 0.000163 |
Lipid Particles | 0.002892 | 0.000782 | 0.001440 | 0.000267 | 0.000771 | 0.001123 | 0.000645 | 0.000387 | 0.001017 | 0.000975 | 0.000704 | 0.000333 |
Mitochondria | 0.001891 | 0.000910 | 0.001638 | 0.002131 | 0.000847 | 0.001972 | 0.000656 | 0.002143 | 0.000968 | 0.001512 | 0.000728 | 0.002138 |
Mitotic Spindle | 0.013121 | 0.005516 | 0.003133 | 0.016378 | 0.001484 | 0.010894 | 0.003187 | 0.008264 | 0.002837 | 0.008563 | 0.003183 | 0.011894 |
None | 0.006243 | 0.011040 | 0.001607 | 0.006515 | 0.004704 | 0.006869 | 0.026278 | 0.005011 | 0.004883 | 0.008677 | 0.024460 | 0.005684 |
Nuclear Periphery | 0.003321 | 0.000476 | 0.002911 | 0.002268 | 0.001011 | 0.000876 | 0.000902 | 0.002853 | 0.001280 | 0.000703 | 0.001050 | 0.002592 |
Nuclear Periphery Foci | 0.003231 | 0.002182 | 0.002354 | 0.000842 | 0.001875 | 0.001437 | 0.000684 | 0.000485 | 0.002033 | 0.001760 | 0.000807 | 0.000645 |
Nucleolus | 0.002535 | 0.000578 | 0.000501 | 0.000527 | 0.000545 | 0.000887 | 0.000873 | 0.000389 | 0.000776 | 0.000753 | 0.000845 | 0.000451 |
Nucleus | 0.605179* | 0.353942* | 0.834571* | 0.866121* | 0.514075* | 0.304939* | 0.749603* | 0.833534* | 0.524672* | 0.326181* | 0.755866* | 0.848115* |
Peroxisomes | 0.002830 | 0.000716 | 0.000218 | 0.000105 | 0.000167 | 0.000832 | 0.000135 | 0.000660 | 0.000476 | 0.000782 | 0.000141 | 0.000412 |
Vacuole | 0.051144 | 0.022127 | 0.068815 | 0.011603 | 0.019740 | 0.028273 | 0.014708 | 0.018456 | 0.023392 | 0.025609 | 0.018696 | 0.015390 |
Vacuole Periphery | 0.000970 | 0.000399 | 0.000238 | 0.000533 | 0.000204 | 0.001317 | 0.000179 | 0.000322 | 0.000293 | 0.000919 | 0.000184 | 0.000416 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 5.13 | -3.03 | 2.99 | -15.98 | 7.74 | 0.58 | -3.30 | -7.67 | -9.37 | -5.94 | 3.35 | -2.85 | -6.11 | -16.12 | -4.05 |
Bud Neck | 7.73 | 5.25 | 4.77 | -15.65 | -1.90 | -3.13 | -6.04 | -11.84 | -7.94 | -8.10 | 2.83 | -0.18 | -10.08 | -13.86 | -10.96 |
Bud Site | 4.26 | 3.79 | 4.13 | -1.70 | 3.02 | -3.22 | -1.16 | -4.32 | -0.60 | -4.14 | 1.00 | 2.55 | 0.31 | -1.10 | -4.23 |
Cell Periphery | 6.05 | 0.28 | 4.70 | -10.16 | 6.56 | 5.07 | -6.93 | -5.15 | -10.94 | 6.36 | 7.20 | -6.66 | -1.07 | -14.96 | 6.55 |
Cytoplasm | -24.30 | 6.86 | 7.74 | 68.06* | 0.05 | -17.25 | 30.33 | 42.93 | 76.31* | 12.37 | -21.20 | 29.19 | 44.05 | 102.07* | 15.29 |
Cytoplasmic Foci | 0.25 | 4.38 | 4.66 | 13.14 | 2.83 | -3.24 | 9.13 | 13.71 | 16.71 | 9.33 | -2.43 | 9.93 | 14.49 | 21.25 | 9.71 |
Eisosomes | 8.27 | 0.31 | 6.19 | -10.61 | 5.87 | 2.34 | -3.69 | -4.13 | -7.26 | 3.59 | 6.30 | -3.64 | -1.46 | -12.37 | 3.60 |
Endoplasmic Reticulum | 3.59 | 1.09 | 2.67 | -14.37 | 5.46 | 6.28 | -2.07 | -8.70 | -18.37 | -7.71 | 5.74 | 0.57 | -1.96 | -23.31 | -5.61 |
Endosome | 2.21 | 2.88 | 4.77 | 2.15 | 3.34 | -4.13 | 0.15 | 1.56 | 4.66 | 1.09 | -2.64 | 2.03 | 3.39 | 4.23 | 1.31 |
Golgi | -1.93 | -1.52 | 2.34 | 2.46 | 3.86 | -2.99 | 0.87 | 0.77 | 3.05 | -0.06 | -3.77 | 1.03 | 0.16 | 3.79 | -0.89 |
Lipid Particles | 3.56 | 1.76 | 4.25 | 2.14 | 6.89 | -1.18 | 1.21 | 2.99 | 2.08 | 2.25 | 0.23 | 2.49 | 5.43 | 2.72 | 4.79 |
Mitochondria | 2.93 | -0.10 | -2.34 | -5.81 | -3.70 | -2.33 | -0.06 | -3.97 | -1.43 | -9.95 | -1.32 | 0.07 | -4.39 | -4.40 | -14.33 |
Mitotic Spindle | 2.05 | 1.50 | 0.28 | -4.79 | -2.16 | -4.72 | -3.42 | -3.81 | 1.59 | 0.19 | -2.97 | -2.11 | -5.08 | -2.17 | -2.29 |
None | -3.46 | 3.09 | -0.54 | 4.61 | -8.72 | -3.37 | -6.78 | 0.55 | 5.12 | 7.03 | -5.80 | -6.62 | -0.88 | 6.57 | 6.54 |
Nuclear Periphery | 3.42 | -0.22 | -0.21 | -23.78 | 0.06 | 0.77 | -5.65 | -7.78 | -7.38 | -6.13 | 3.92 | -2.81 | -10.50 | -14.21 | -9.96 |
Nuclear Periphery Foci | 1.32 | 0.62 | 3.83 | 4.94 | 2.10 | 1.97 | 4.06 | 6.21 | 5.69 | 1.94 | 1.24 | 4.25 | 6.96 | 7.17 | 2.40 |
Nucleolus | 3.23 | 3.23 | 2.81 | -1.88 | -2.45 | -1.15 | -1.57 | 0.72 | 1.59 | 2.82 | -0.04 | -0.34 | 1.04 | 0.87 | 1.65 |
Nucleus | 9.52 | -6.21 | -11.17 | -59.96* | -4.23 | 20.62 | -25.08 | -36.27 | -72.90* | -9.10 | 21.61 | -25.46 | -40.16 | -94.11* | -11.66 |
Peroxisomes | 2.42 | 3.38 | 3.69 | 3.02 | 6.82 | -3.19 | 1.04 | -0.64 | 1.67 | -0.79 | -2.34 | 3.47 | 1.23 | 2.87 | -0.54 |
Vacuole | 4.85 | -0.97 | 4.97 | 0.52 | 5.76 | -3.43 | -1.59 | -2.60 | 0.83 | -0.75 | -0.18 | -0.89 | 0.53 | 0.98 | 1.57 |
Vacuole Periphery | 1.48 | 2.06 | 2.28 | 1.16 | 1.03 | -2.89 | 0.56 | -0.09 | 2.87 | -0.64 | -2.76 | 1.29 | 0.84 | 3.08 | -0.61 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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