Standard name
Description Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.77 0.90 0.79 0.31 0.43 0.63 0.47 0.47 0.42 0.40 0.37 0.35 0.35 0.33 0.35 0.65 0.57 0.54 0.77 0.67 0.81 0.60 0.36 0.34
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.28 0.12 0.25 0.32 0.31 0.51 0.52 0.59 0.61 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.07 0 0 0.11 0.11 0.07 0.08 0.13 0.11 0.11 0.12 0.13 0.17 0.22 0.24 0.10 0.16 0.09 0.19 0.23 0.07 0.28 0.37 0.29
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.39 0.18 0.21 0.46 0.51 0.25 0.35 0.32 0.23 0.19 0.17 0.20 0.69 0.56 0.47 0.52 0.47 0.54 0 0.05 0 0.06 0.12 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1
Bud 0 0 6 6 3 0 3 5 5 9 8 4 0 0 2 1 0 0 0 0 1 3 15 18
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2
Bud Site 0 0 0 1 0 0 0 2 0 2 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 2 1 1 0 3 3 3 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 68 110 111 54 126 144 208 230 129 156 76 76 47 80 97 82 92 107 93 83 124 80 115 122
Endoplasmic Reticulum 0 0 1 0 0 1 0 1 1 2 1 2 0 1 0 3 8 11 0 0 0 0 2 6
Endosome 0 0 0 6 13 0 9 8 0 1 0 0 2 2 2 3 2 2 0 1 1 2 10 12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 5 10 0 0 1 0 0 1
Mitochondria 0 2 11 48 35 57 140 153 156 201 122 135 1 1 5 3 2 5 0 1 0 0 0 2
Nucleus 6 4 2 19 31 15 35 62 35 44 25 28 22 54 67 13 25 18 22 28 11 37 118 103
Nuclear Periphery 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 3
Nucleolus 0 0 0 0 0 0 1 1 0 0 0 0 0 1 1 1 0 0 0 0 0 0 1 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 8 11
Vac/Vac Membrane 34 22 29 80 151 57 155 156 70 72 35 45 92 136 128 66 75 106 1 6 5 7 39 59
Unique Cell Count 88 122 141 173 295 229 443 492 305 387 208 220 133 242 275 127 160 197 121 126 155 135 321 354
Labelled Cell Count 108 138 160 214 359 276 553 620 396 490 270 294 164 275 302 173 211 259 121 126 155 135 321 354

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm, Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.5 6.5 5.9 5.4 5.8 5.4 5.3 5.4 4.8 4.7 4.7 4.4 8.5 8.6 7.6 11.5 12.8 13.1 8.5 8.3 9.2
Std Deviation (1e-4) 1.2 1.2 1.1 0.8 1.3 1.1 1.3 1.4 1.2 1.1 1.2 1.5 1.3 1.5 1.3 2.2 2.8 2.9 1.3 1.5 1.7
Intensity Change (Log2) -0.13 -0.05 -0.13 -0.18 -0.14 -0.32 -0.34 -0.35 -0.43 0.52 0.54 0.35 0.95 1.1 1.14 0.52 0.48 0.63

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -0.4 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 0.7 1.1 0.1 0.8 0 0 0 0 2.9 1.5 -0.1 7.2 7.2 6.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.5 1.3 4.1 5.6 5.6 0 0 0 0 -2.8 0 -3.0 -2.0 -2.8 -2.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.3 4.0 1.0 2.0 1.3 0 0 0 0 6.7 5.7 3.2 4.5 4.3 5.6