Standard name
Human Ortholog
Description Phosphatidylinositol:ceramide phosphoinositol transferase; required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance; also known as IPC synthase

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.08 0.13 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.12 0.11 0.17 0 0.06 0 0 0 0.05
Bud 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.07 0.08 0.05 0.05 0 0 0 0 0 0 0 0 0.05 0.08 0.07 0.07 0.08 0.08 0.06 0.09 0.07 0.06
Golgi 0.7 0.7 0.38 0.32 0.2 0.05 0 0.07 0.07 0.07 0 0.05 0.79 0.75 0.75 0.73 0.69 0.73 0.84 0.73 0.82 0.77 0.8 0.81
Mitochondria 0.63 0.56 0.86 0.79 0.86 0.88 0.89 0.87 0.87 0.87 0.95 0.92 0.39 0.34 0.24 0.1 0.1 0.11 0 0.05 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.08 0 0.05 0 0 0 0 0.05 0 0 0 0.12 0.2 0.22 0.09 0.11 0.12 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 39 64 11 6 7 26 12 18 11 17 17 27 28 8 6 33 28 44 9 34 22 17 15 21
Bud 5 3 15 9 15 57 34 29 30 37 31 36 1 2 1 2 3 3 0 4 0 2 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 6 6 3 5 7 2 8 0 0 0 0 0 0
Cell Periphery 2 1 3 1 3 11 0 7 6 2 7 9 2 1 1 3 1 3 0 0 0 0 0 0
Cytoplasm 14 22 8 14 31 21 19 15 28 20 10 23 9 8 13 11 22 13 1 1 1 2 3 2
Endoplasmic Reticulum 10 25 8 2 13 12 18 8 16 16 19 32 13 11 3 5 9 4 2 2 2 3 2 2
Endosome 10 7 43 29 32 42 43 43 12 38 7 9 16 18 21 20 17 19 39 45 40 36 29 25
Golgi 335 335 224 121 125 38 42 74 55 56 28 52 355 356 329 194 172 185 445 399 521 311 344 321
Mitochondria 302 268 506 297 548 684 876 911 641 742 708 899 175 160 106 26 26 27 11 27 23 14 13 11
Nucleus 0 2 0 0 0 5 4 1 4 1 3 3 0 1 1 0 1 1 0 1 0 0 0 0
Nuclear Periphery 0 0 2 0 6 5 11 6 6 10 12 8 1 5 3 0 1 3 1 2 2 2 0 1
Nucleolus 11 3 6 11 17 14 33 76 27 35 25 39 6 1 5 11 10 8 0 0 0 0 0 1
Peroxisomes 0 0 2 4 5 25 12 10 5 12 3 14 2 3 1 2 0 1 3 1 8 3 6 3
SpindlePole 2 1 2 2 3 7 12 8 7 12 3 10 0 2 1 3 7 2 1 1 0 0 0 0
Vac/Vac Membrane 58 39 21 20 22 25 39 41 34 29 20 32 56 93 94 25 27 31 9 19 5 6 8 4
Unique Cell Count 479 479 587 374 635 781 985 1042 737 854 748 975 449 473 436 266 248 254 529 546 635 404 429 399
Labelled Cell Count 788 770 851 516 827 978 1161 1250 887 1034 895 1201 664 669 585 335 324 344 529 546 635 404 429 399


Early Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.5 15.5 11.1 10.5 9.1 9.0 8.3 8.8 9.3 8.7 8.8 8.8 14.7 13.8 13.0 15.2 14.8 15.1 15.6 14.4 14.3
Std Deviation (1e-4) 2.6 3.3 1.9 1.9 1.7 1.7 1.8 1.9 2.5 1.8 1.8 1.7 2.6 2.5 2.5 3.5 3.3 3.6 2.7 2.8 2.4
Intensity Change (Log2) -0.08 -0.29 -0.3 -0.42 -0.34 -0.26 -0.36 -0.34 -0.33 0.41 0.31 0.23 0.46 0.42 0.45 0.49 0.38 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.3 -1.1 1.6 -1.0 -0.2 -0.5 0.2 0.5 1.1 3.7 -0.2 -0.6 6.4 5.9 8.3
Bud -0.1 -0.2 3.9 1.0 0.3 1.5 1.8 1.6 1.2 -3.0 -2.7 -3.0 -1.7 -1.2 -1.3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.6 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.4 3.5 1.7 0.8 0.1 2.7 1.3 0 1.4 0.8 0.4 1.8 2.5 5.3 3.2
Endoplasmic Reticulum 0 0.9 0.3 0.7 -1.2 1.1 0.7 1.5 2.3 1.7 1.2 0 0 2.1 0
Endosome 0.2 -1.7 -1.5 -2.5 -2.8 -5.2 -2.3 -6.1 -6.8 -2.6 -2.4 -1.6 0.1 -0.2 0.1
Golgi -1.8 -7.1 -15.5 -17.3 -15.6 -13.6 -14.9 -16.0 -16.5 13.1 12.1 11.8 9.4 8.2 9.2
Mitochondria -2.8 0 0.8 1.6 0.7 0.4 0.4 5.3 3.8 -15.9 -17.5 -20.0 -21.3 -20.8 -20.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 2.2 0 0 0 0 0 0 0
Nucleolus 2.2 2.1 1.2 2.9 5.6 3.1 3.5 2.8 3.4 0.5 0 0 3.0 0 0
Peroxisomes 0 0 3.8 1.8 0 0 2.0 0 2.1 0 0 0 0 0 0
SpindlePole 0 0 0 1.8 0 0 2.0 0 0 0 0 0 0 0 0
Vacuole 1.3 -0.1 -0.4 0.4 0.4 0.9 -0.2 -0.9 -0.3 5.4 8.4 9.0 3.5 4.1 4.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.6325 9.7841 9.0556 9.6097 9.1419 9.2449 12.1015 14.1081 13.2642 13.4941 12.8299 13.5291 9.2625 11.7092 10.3713 9.5734 10.699 10.1877
Actin 0.0049 0.014 0.0023 0.0011 0.0005 0.0045 0.0049 0.0014 0.0016 0.0001 0.0006 0.0017 0.0093 0.0019 0.0034 0.0013 0.0004 0.0046
Bud 0.0001 0.0024 0.0007 0 0 0.0004 0 0 0 0 0 0 0.0001 0.0001 0 0.0001 0 0
Bud Neck 0.0002 0.0002 0.0003 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0 0 0.0001 0.0002 0.0002 0.0001 0.0001 0 0.0001
Bud Periphery 0.0001 0.0006 0.0002 0 0 0.0002 0 0 0 0 0 0 0.0002 0.0001 0 0.0001 0 0.0001
Bud Site 0.0002 0.0023 0.0004 0 0 0.0002 0.0001 0 0 0 0 0 0.0003 0.0004 0.0001 0.0001 0 0
Cell Periphery 0.0001 0.0002 0.0001 0 0 0 0 0 0 0 0 0 0.0001 0 0 0.0001 0 0
Cytoplasm 0.0039 0.0033 0.0021 0.0008 0.001 0.002 0.0008 0.001 0.001 0.0002 0.0005 0.0006 0.0011 0.0062 0.0008 0.0013 0.0003 0.0005
Cytoplasmic Foci 0.0528 0.0593 0.0569 0.0377 0.0536 0.0372 0.0424 0.0454 0.0351 0.0166 0.0303 0.0274 0.0411 0.0349 0.0285 0.0204 0.028 0.0185
Eisosomes 0.0001 0 0 0 0 0 0 0 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0032 0.0005 0.0014 0.0003 0.0003 0.0044 0.0004 0.0002 0.0002 0.0003 0.0001 0.0012 0.0017 0.0013 0.0007 0.0013 0.0002 0.0009
Endosome 0.1914 0.1676 0.1518 0.1685 0.1801 0.1122 0.103 0.084 0.0884 0.1447 0.0703 0.074 0.1609 0.141 0.1256 0.1966 0.1244 0.1109
Golgi 0.6798 0.7243 0.7488 0.7607 0.7606 0.801 0.8203 0.8599 0.8445 0.7986 0.8897 0.8764 0.6958 0.7726 0.8017 0.7316 0.8288 0.8281
Lipid Particles 0.01 0.0026 0.0041 0.0203 0.0012 0.0026 0.0031 0.0005 0.0009 0.0018 0.0008 0.0014 0.0242 0.0028 0.003 0.0055 0.0009 0.003
Mitochondria 0.0415 0.0171 0.0215 0.0071 0.0008 0.028 0.0191 0.0063 0.0261 0.0299 0.0068 0.0152 0.0552 0.0336 0.033 0.0311 0.0149 0.0265
None 0.0006 0.0004 0.0003 0 0 0.0001 0 0 0 0 0 0 0.0001 0 0 0 0 0
Nuclear Periphery 0.0008 0.0001 0.0001 0.0001 0 0.0003 0.0001 0 0 0.0001 0 0 0.0018 0.0002 0.0001 0.0002 0 0.0003
Nucleolus 0 0.0002 0.0003 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.0001 0.0001 0.0006 0 0 0.0001 0 0 0 0 0 0 0.0002 0.0001 0 0 0 0
Peroxisomes 0.0055 0.0012 0.0026 0.0004 0.0003 0.0038 0.0042 0.0005 0.0009 0.0003 0.0002 0.0009 0.0027 0.0005 0.0007 0.0004 0.0007 0.0005
Punctate Nuclear 0.0007 0.0001 0.0008 0 0 0.0001 0 0 0 0 0 0 0.0001 0 0 0 0 0
Vacuole 0.0018 0.0017 0.0021 0.0009 0.0005 0.0008 0.0004 0.0002 0.0003 0.0018 0.0001 0.0003 0.0015 0.0019 0.0006 0.0024 0.0002 0.0016
Vacuole Periphery 0.0021 0.0018 0.0024 0.0019 0.0008 0.0016 0.0011 0.0004 0.0008 0.0055 0.0005 0.0007 0.0034 0.002 0.0014 0.0074 0.0011 0.004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 75.9391 114.5047 67.367 81.8695 58.126 41.149 99.0295 89.5827 64.0967 87.6046
Translational Efficiency 0.6868 0.57 0.8753 0.4877 0.7193 1.1331 0.7342 0.6712 0.7933 0.5372

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1662 2272 764 1590 1895 1590 140 1543 3557 3862 904 3133

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1020.80 1273.78 1571.02 1367.69 1118.03 1109.80 1593.99 1392.45 1072.60 1206.27 1574.58 1379.88
Standard Deviation 187.27 208.63 220.70 199.32 173.58 175.85 203.73 194.60 186.53 211.78 218.32 197.40
Intensity Change Log 2 0.319416 0.622001 0.422041 -0.010659 0.511684 0.316667 0.156308 0.565388 0.367920

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.010283 0.004873 0.004019 0.008943 0.003037 0.003317 0.009978 0.010644 0.006423 0.004232 0.004942 0.009781
Bud Neck 0.000246 0.000173 0.001007 0.000651 0.000121 0.000211 0.001184 0.000210 0.000179 0.000189 0.001034 0.000434
Bud Site 0.001746 0.001030 0.001343 0.002075 0.000822 0.000614 0.001649 0.001976 0.001254 0.000859 0.001390 0.002026
Cell Periphery 0.000183 0.000064 0.000138 0.000145 0.000086 0.000092 0.000226 0.000178 0.000132 0.000075 0.000152 0.000161
Cytoplasm 0.001004 0.000525 0.000454 0.000416 0.000507 0.000727 0.000183 0.000719 0.000739 0.000608 0.000412 0.000566
Cytoplasmic Foci 0.131959 0.148056 0.044919 0.108490 0.156699 0.189735* 0.091555 0.090078 0.145139 0.165215 0.052141 0.099422
Eisosomes 0.000144 0.000081 0.000043 0.000085 0.000066 0.000064 0.000108 0.000125 0.000102 0.000074 0.000053 0.000105
Endoplasmic Reticulum 0.001470 0.000796 0.002223 0.004061 0.001162 0.000986 0.002632 0.004028 0.001306 0.000874 0.002287 0.004045
Endosome 0.127430 0.096902 0.043698 0.104386 0.105138 0.139427 0.084872 0.077152 0.115554 0.114410 0.050074 0.090973
Golgi 0.632342* 0.693998* 0.800096* 0.662932* 0.662151* 0.594440* 0.725191* 0.754701* 0.648223* 0.653010* 0.788496* 0.708128*
Lipid Particles 0.020783 0.013266 0.019387 0.045055 0.009624 0.026684 0.054053 0.036751 0.014838 0.018790 0.024756 0.040966
Mitochondria 0.054943 0.025426 0.007191 0.011820 0.044669 0.023925 0.002986 0.006713 0.049470 0.024808 0.006540 0.009305
Mitotic Spindle 0.000346 0.001101 0.003576 0.002153 0.000411 0.000747 0.000937 0.000354 0.000381 0.000955 0.003167 0.001267
None 0.000514 0.000046 0.000078 0.000059 0.000061 0.000203 0.000053 0.000044 0.000273 0.000111 0.000074 0.000052
Nuclear Periphery 0.000085 0.000106 0.001460 0.000650 0.000087 0.000165 0.000821 0.000135 0.000086 0.000130 0.001361 0.000396
Nuclear Periphery Foci 0.000450 0.000256 0.004162 0.002679 0.000302 0.001273 0.001159 0.000549 0.000371 0.000675 0.003697 0.001630
Nucleolus 0.000584 0.000005 0.000015 0.000009 0.000012 0.000135 0.000007 0.000004 0.000279 0.000059 0.000014 0.000007
Nucleus 0.000640 0.000013 0.000024 0.000019 0.000023 0.000242 0.000019 0.000014 0.000311 0.000107 0.000023 0.000017
Peroxisomes 0.001729 0.000570 0.000260 0.000456 0.000729 0.001553 0.000856 0.000283 0.001196 0.000975 0.000353 0.000371
Vacuole 0.004392 0.004660 0.033875 0.025810 0.004559 0.006894 0.012588 0.007605 0.004481 0.005580 0.030578 0.016844
Vacuole Periphery 0.008727 0.008054 0.032033 0.019106 0.009735 0.008565 0.008943 0.007736 0.009264 0.008265 0.028457 0.013506

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.67 3.69 0.73 -2.91 -3.01 -0.43 -1.73 -5.02 -4.89 -0.16 2.77 1.15 -2.89 -5.36 -3.35
Bud Neck 1.35 -5.19 -4.26 -4.82 2.08 -2.90 -2.44 -3.28 -0.01 2.23 -0.31 -6.13 -5.10 -4.53 4.08
Bud Site 1.47 0.70 -0.72 -2.62 -1.69 0.94 -1.64 -2.53 -3.24 -0.57 1.43 -0.58 -2.43 -3.88 -1.68
Cell Periphery 5.57 1.93 1.47 -5.35 -0.40 -0.56 -2.36 -2.75 -2.55 0.71 5.05 -1.27 -1.46 -4.71 -0.45
Cytoplasm 1.58 1.21 2.21 0.84 0.33 -0.83 3.40 -1.11 -0.46 -1.95 0.61 0.94 0.22 -0.27 -0.68
Cytoplasmic Foci -4.34 24.19 6.11 10.09 -16.64 -7.18 6.38 18.42 23.41 0.13 -6.85 28.14 17.13 23.01 -14.60
Eisosomes 4.52 7.11 3.95 -0.47 -4.37 0.32 -1.74 -3.30 -3.37 -0.56 3.93 5.49 -0.22 -3.04 -4.50
Endoplasmic Reticulum 3.02 -1.90 -3.35 -4.33 -2.24 0.48 -2.23 -3.88 -4.54 -1.61 1.98 -2.70 -5.09 -6.28 -3.03
Endosome 7.63 20.86 5.20 -2.03 -16.21 -7.93 2.46 8.05 14.77 0.97 0.40 20.97 8.74 8.45 -13.53
Golgi -8.32 -16.88 -3.46 4.00 13.35 8.69 -2.96 -12.63 -19.94 -1.38 -0.89 -16.57 -10.37 -9.63 9.31
Lipid Particles 3.43 0.44 -6.89 -10.11 -6.60 -6.50 -3.79 -10.06 -2.93 1.47 -2.48 -3.29 -11.82 -9.83 -4.72
Mitochondria 7.11 12.12 10.75 5.32 -2.10 5.43 12.53 11.40 7.50 -2.83 8.77 15.82 15.47 8.97 -1.76
Mitotic Spindle -2.49 -2.90 -4.27 -2.12 1.20 -1.68 -0.73 0.29 1.73 0.79 -2.93 -2.94 -3.86 -1.12 1.96
None 1.77 1.65 1.72 -2.35 2.59 -1.09 0.61 3.20 1.21 0.66 1.19 1.61 1.79 1.10 3.73
Nuclear Periphery -0.97 -6.64 -8.79 -8.43 3.76 -2.79 -1.83 -2.94 1.05 1.71 -2.65 -6.87 -9.17 -7.47 5.13
Nuclear Periphery Foci 1.48 -3.91 -4.70 -5.29 1.40 -2.22 -2.45 -2.49 1.56 1.62 -1.56 -4.16 -5.16 -3.27 2.45
Nucleolus 1.09 1.07 1.08 -1.58 1.82 -1.67 0.85 1.09 1.77 0.75 0.87 1.07 1.09 1.71 2.57
Nucleus 1.45 1.43 1.44 -1.61 1.14 -1.73 0.50 0.74 1.78 0.32 0.97 1.43 1.46 1.72 1.47
Peroxisomes 5.11 6.44 5.43 1.13 -1.92 -2.73 -0.31 2.95 4.70 1.49 1.29 5.61 6.04 4.85 -0.20
Vacuole -0.46 -11.12 -12.24 -12.22 2.58 -3.51 -3.20 -3.49 -0.85 1.83 -2.61 -11.48 -12.35 -11.10 5.55
Vacuole Periphery 0.99 -10.63 -8.48 -9.41 5.40 1.55 0.43 2.62 1.07 0.65 2.00 -10.23 -5.80 -7.38 7.70