Standard name
Human Ortholog
Description Class E Vps protein of the ESCRT-III complex; required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.06 0 0.06 0.07
Bud 0 0 0 0 0 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.07 0 0 0.08 0 0 0 0.07 0.09 0.12 0.13 0.11 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.32 0.39 0.72 0.71 0.3 0.19 0.32 0.35 0.4 0.48 0.65 0.49 0.44 0.51 0.73 0.57 0.68 0.54 0.55 0.52
Golgi 0 0 0 0.06 0.05 0.12 0.05 0 0 0 0 0 0.08 0 0.07 0.05 0.07 0.1 0.12 0.14
Mitochondria 0.5 0.37 0.2 0.27 0.65 0.88 0.58 0.7 0.38 0.13 0.16 0.09 0.13 0.17 0.06 0.09 0 0.11 0.05 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0.06 0 0 0 0 0 0 0
Nucleolus 0.11 0.15 0.08 0.06 0.24 0.25 0.13 0.1 0.19 0 0.06 0.4 0.34 0.3 0 0 0 0 0 0
Peroxisomes 0.07 0.08 0.12 0.1 0 0.12 0 0 0.05 0.13 0.14 0 0.05 0 0.05 0 0.09 0 0.1 0.07
SpindlePole 0.13 0.12 0.12 0.1 0 0 0.08 0.05 0.1 0.26 0.2 0.17 0.18 0.3 0 0 0 0 0 0
Vac/Vac Membrane 0 0.11 0 0.21 0.08 0 0.08 0.1 0 0.09 0 0.12 0.1 0.08 0 0.11 0 0.08 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 1 0 1 0 0 1 1 0 0 0 0 0 0 5 8 3 1 5 6
Bud 4 6 0 0 1 1 1 0 2 1 0 3 2 0 0 2 0 1 0 0
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 12 1 2 3 0 1 0 3 2 6 17 14 7 0 1 0 1 1 0
Endoplasmic Reticulum 1 3 1 0 1 0 0 0 1 0 1 3 4 2 0 2 0 0 0 0
Endosome 38 66 18 34 11 3 12 7 17 11 32 63 55 43 106 124 41 24 49 46
Golgi 2 4 0 3 2 2 2 0 1 1 0 2 10 3 9 11 4 4 10 12
Mitochondria 60 63 5 13 24 14 22 14 16 3 8 11 16 14 8 19 2 5 4 5
Nucleus 1 3 0 1 0 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 2 1 0 0 0 3 7 1 0 2 0 0 2 1
Nucleolus 13 25 2 3 9 4 5 2 8 1 3 51 42 25 0 2 0 0 0 0
Peroxisomes 8 14 3 5 1 2 0 0 2 3 7 5 6 2 6 8 5 1 8 5
SpindlePole 15 20 3 5 1 0 3 1 4 6 10 22 22 25 2 5 0 0 1 1
Vac/Vac Membrane 5 19 0 10 3 0 3 2 1 2 2 15 13 7 2 23 0 3 3 4
Unique Cell Count 119 171 25 48 37 16 38 20 42 23 49 129 124 84 145 219 62 46 89 91
Labelled Cell Count 151 238 34 77 56 26 52 28 55 30 69 197 192 130 145 219 62 46 89 91


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 6.8 6.8 10.1 10.3 9.8 10.5 9.0 6.6 8.5 7.6 9.7 12.4 9.9 8.1 8.5 9.3
Std Deviation (1e-4) 1.6 1.8 1.5 2.5 2.7 2.8 3.6 2.3 1.7 1.8 1.5 2.7 3.6 3.5 2.0 2.8 2.6
Intensity Change (Log2) 0.57 0.59 0.52 0.63 0.41 -0.06 0.31 0.15 0.5 0.86 0.53 0.25 0.32 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0
Endosome -0.1 -3.3 -3.3 -3.1 -2.5 -2.5 -1.7 -0.6 -2.1 -2.5 -1.8
Golgi 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.7 3.5 4.2 3.0 3.4 1.5 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 3.0 2.6 2.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1.8
Vacuole 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6365 2.8202 2.6053 1.1441 1.3952 1.9787 4.2752 7.7452 7.7644 7.2538 6.6451 7.2635 3.3576 6.2174 4.7219 6.7671 3.8455 4.0394
Actin 0.0542 0.0047 0.0209 0.0438 0.01 0.0074 0.0005 0.0423 0.0006 0.0001 0.0003 0.0016 0.0326 0.0009 0.001 0.0008 0 0.0024
Bud 0.0018 0.0012 0.0003 0.0012 0.0003 0.0027 0 0.0005 0.0002 0 0 0 0.0022 0.0003 0.0011 0.0002 0 0.0001
Bud Neck 0.0064 0.0009 0.0013 0.0049 0.0006 0.0005 0.0002 0.001 0.0007 0.0002 0.0003 0.0003 0.0008 0.0029 0.0006 0.0075 0 0.0006
Bud Periphery 0.0027 0.0013 0.0004 0.0022 0.0005 0.0017 0 0.0005 0.0003 0 0 0.0001 0.0034 0.0003 0.0026 0.0004 0 0.0003
Bud Site 0.0086 0.0032 0.0013 0.0247 0.0008 0.0007 0.0001 0.0024 0.0008 0.0001 0.0001 0.0002 0.008 0.0059 0.0018 0.0037 0 0.0004
Cell Periphery 0.0011 0.0005 0.0006 0.0013 0.0002 0.0002 0.0001 0.0006 0.0002 0 0.0001 0.0002 0.0016 0.0006 0.0011 0.0009 0 0.0013
Cytoplasm 0.0322 0.005 0.0025 0.0012 0.0098 0.0181 0.0006 0.0057 0.0011 0.0015 0.0008 0.0005 0.0202 0.0013 0.0042 0.0056 0.0011 0.0013
Cytoplasmic Foci 0.1133 0.1318 0.155 0.0682 0.0949 0.1378 0.1398 0.1442 0.1406 0.1255 0.1254 0.0821 0.0798 0.2328 0.2073 0.1697 0.034 0.097
Eisosomes 0.0003 0 0.0001 0.0003 0.0001 0 0 0.0001 0 0 0 0 0.0025 0 0 0.0006 0 0.0001
Endoplasmic Reticulum 0.0083 0.0015 0.0068 0.0007 0.0058 0.003 0.0003 0.0009 0.0008 0.0003 0.0003 0.0003 0.0133 0.0004 0.0046 0.0041 0.0002 0.005
Endosome 0.2125 0.3458 0.313 0.3209 0.3256 0.4108 0.2034 0.2688 0.3076 0.3382 0.282 0.1658 0.1622 0.1938 0.1702 0.3408 0.4635 0.2408
Golgi 0.0796 0.1383 0.1181 0.0492 0.1366 0.1039 0.1036 0.2123 0.1099 0.0615 0.1251 0.1011 0.0476 0.0863 0.0678 0.1194 0.0203 0.1515
Lipid Particles 0.2139 0.2697 0.2423 0.2853 0.2966 0.2045 0.4151 0.1016 0.2493 0.4396 0.433 0.5642 0.2065 0.2657 0.3511 0.161 0.4584 0.3086
Mitochondria 0.0795 0.0142 0.014 0.0626 0.0333 0.0061 0.0256 0.0487 0.0627 0.0012 0.0026 0.0203 0.0455 0.0109 0.0615 0.0042 0.0006 0.0486
None 0.0252 0.0051 0.0003 0.0002 0.0026 0.0246 0.0001 0.0003 0.0003 0.0001 0.0001 0.0001 0.3105 0.0002 0.0007 0.0421 0 0.0007
Nuclear Periphery 0.0329 0.0008 0.0129 0.0004 0.0312 0.0022 0.0002 0.0006 0.0004 0.0002 0.0002 0.0003 0.0095 0.0003 0.0024 0.0028 0.0002 0.0113
Nucleolus 0.0027 0.0021 0.0081 0.001 0.0008 0.0003 0.0002 0.0006 0.0005 0.0002 0.0001 0.0006 0.0013 0.002 0.0009 0.0064 0 0.0013
Nucleus 0.02 0.0005 0.0011 0.0006 0.0077 0.0013 0.0001 0.0004 0.0002 0.0001 0 0.0001 0.0013 0.0004 0.0007 0.002 0 0.0007
Peroxisomes 0.0434 0.0329 0.0444 0.0356 0.0144 0.045 0.1053 0.0517 0.1073 0.0222 0.0202 0.0503 0.0281 0.1705 0.0903 0.0238 0.0103 0.0315
Punctate Nuclear 0.0087 0.0022 0.0013 0.0008 0.0071 0.0014 0.0006 0.0004 0.0011 0.0021 0.0006 0.0007 0.0082 0.0011 0.001 0.0172 0.0002 0.0006
Vacuole 0.0394 0.0296 0.042 0.0878 0.0125 0.0177 0.0024 0.1059 0.0115 0.0042 0.0037 0.0072 0.0108 0.0208 0.0224 0.072 0.0083 0.0782
Vacuole Periphery 0.0132 0.0087 0.0135 0.0071 0.0083 0.01 0.0017 0.0107 0.0039 0.0027 0.005 0.0039 0.004 0.0024 0.0069 0.0146 0.0028 0.0179

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.6744 33.867 28.2721 34.8164 45.0264 44.1749 33.5295 38.0379 47.5023 34.5765
Translational Efficiency 1.498 1.5968 1.5353 1.83 1.181 1.6613 1.5543 1.5826 1.404 1.4822

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
89 302 1651 1066 994 752 1609 620 1083 1054 3260 1686

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1275.80 910.29 1027.63 903.30 808.78 915.27 930.18 944.26 847.16 913.84 979.53 918.36
Standard Deviation 344.20 175.43 187.64 192.94 135.38 141.14 235.88 228.14 207.38 151.77 218.32 207.52
Intensity Change Log 2 -0.487004 -0.312081 -0.498125 0.178450 0.201763 0.223437 -0.191418 -0.090516 -0.174136

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.097993 0.010247 0.004017 0.006697 0.017776 0.010406 0.007784 0.006874 0.024368 0.010361 0.005876 0.006762
Bud Neck 0.005719 0.018548 0.017417 0.008353 0.012389 0.022485 0.014904 0.008345 0.011841 0.021357 0.016177 0.008350
Bud Site 0.007980 0.020124 0.024056 0.025979 0.013403 0.019472 0.043795 0.021810 0.012958 0.019659 0.033798 0.024446
Cell Periphery 0.003993 0.001041 0.000482 0.000266 0.001719 0.002621 0.000492 0.000474 0.001906 0.002169 0.000487 0.000342
Cytoplasm 0.007184 0.013728 0.005652 0.025909 0.016144 0.026724 0.031512 0.016874 0.015407 0.023000 0.018415 0.022586
Cytoplasmic Foci 0.033333 0.111161 0.100591 0.090826 0.073214 0.113603 0.104136 0.103861 0.069937 0.112903 0.102340 0.095619
Eisosomes 0.003395 0.000410 0.000136 0.000068 0.000369 0.000849 0.000098 0.000092 0.000617 0.000724 0.000118 0.000077
Endoplasmic Reticulum 0.010232 0.001373 0.001219 0.001293 0.001540 0.003307 0.001029 0.002118 0.002255 0.002753 0.001125 0.001596
Endosome 0.278367* 0.556750* 0.482051* 0.466589* 0.576788* 0.475908* 0.451302* 0.457406* 0.552264* 0.499071* 0.466874* 0.463212*
Golgi 0.431435* 0.083793 0.064706 0.084544 0.122572* 0.096409 0.070930 0.113344* 0.147954* 0.092794 0.067778 0.095135
Lipid Particles 0.041220 0.044908 0.055304 0.022939 0.059357 0.073064 0.023623 0.058153 0.057866 0.064996 0.039667 0.035888
Mitochondria 0.031149 0.017063 0.036516 0.017604 0.044418 0.035834 0.027692 0.015528 0.043328 0.030456 0.032161 0.016841
Mitotic Spindle 0.000879 0.012391 0.020897 0.040354 0.001069 0.004172 0.041067 0.014712 0.001053 0.006527 0.030852 0.030924
None 0.000123 0.000257 0.000254 0.000710 0.001328 0.001867 0.000411 0.002388 0.001229 0.001406 0.000332 0.001327
Nuclear Periphery 0.000153 0.000217 0.001911 0.001472 0.000338 0.000411 0.001318 0.001136 0.000323 0.000356 0.001618 0.001348
Nuclear Periphery Foci 0.000264 0.002033 0.011281 0.007095 0.001040 0.002401 0.008383 0.013019 0.000976 0.002295 0.009851 0.009274
Nucleolus 0.000042 0.000353 0.002680 0.000958 0.000212 0.001045 0.000987 0.001273 0.000198 0.000847 0.001844 0.001073
Nucleus 0.000204 0.002975 0.002881 0.004493 0.001527 0.006548 0.005339 0.004689 0.001418 0.005524 0.004094 0.004565
Peroxisomes 0.012512 0.037562 0.034342 0.019791 0.030379 0.057027 0.035261 0.020058 0.028911 0.051450 0.034796 0.019889
Vacuole 0.019264 0.054742 0.112436 0.145666 0.018810 0.037837 0.107948 0.113395 0.018848 0.042681 0.110221 0.133799
Vacuole Periphery 0.014557 0.010324 0.021171 0.028396 0.005610 0.008010 0.021990 0.024453 0.006345 0.008673 0.021575 0.026946

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.66 5.05 4.89 1.15 -1.76 2.46 3.89 3.70 1.36 0.45 4.47 6.67 6.04 1.89 -0.65
Bud Neck -3.07 -7.12 -1.91 2.45 6.93 -3.51 -1.80 2.30 5.26 5.05 -3.82 -3.20 2.24 5.81 8.44
Bud Site -2.56 -6.24 -6.22 -1.59 -0.96 -1.76 -10.27 -2.97 -0.95 5.54 -2.31 -10.01 -5.52 -2.17 3.53
Cell Periphery 2.42 2.94 3.12 3.06 4.74 -1.16 5.09 5.13 2.90 0.16 -0.45 5.91 6.50 3.43 3.31
Cytoplasm -1.14 -0.04 -4.20 -4.04 -6.79 -2.51 -6.51 -0.94 1.32 4.67 -2.17 -3.08 -3.67 -1.54 -1.35
Cytoplasmic Foci -6.89 -7.98 -6.72 2.33 2.05 -6.31 -6.17 -4.83 1.34 -0.10 -7.69 -7.78 -5.65 3.32 1.70
Eisosomes 3.16 3.50 3.57 2.32 6.39 -2.45 8.55 8.68 3.90 0.62 -0.63 5.85 6.32 4.46 5.40
Endoplasmic Reticulum 4.35 4.40 4.36 -0.01 -0.33 -2.54 1.64 -1.78 1.64 -3.46 -0.92 3.67 1.99 2.19 -2.73
Endosome -9.22 -7.74 -6.43 5.52 2.46 6.74 12.22 8.70 1.79 -1.31 4.00 9.55 9.03 4.27 0.56
Golgi 10.19 10.96 10.41 0.44 -3.40 3.77 9.66 1.57 -1.64 -5.96 7.81 13.53 8.34 0.29 -6.31
Lipid Particles -0.27 -1.19 1.67 2.78 8.18 -1.89 7.15 0.29 2.01 -5.76 -1.13 3.92 4.35 5.68 1.10
Mitochondria 1.36 -0.46 1.42 0.04 6.51 1.65 4.11 6.52 5.06 3.52 2.85 3.06 6.91 4.81 7.03
Mitotic Spindle -2.43 -9.06 -9.72 -4.77 -4.23 -3.23 -12.62 -4.64 -3.56 6.09 -3.59 -15.52 -10.74 -7.96 0.17
None -1.18 -2.62 -2.36 -1.62 -1.79 -0.49 1.12 -0.79 -0.38 -1.51 -0.24 1.22 -0.24 0.02 -1.96
Nuclear Periphery -0.94 -8.29 -6.04 -5.63 1.47 -0.29 -3.41 -2.45 -3.03 0.84 -0.15 -5.17 -3.86 -5.82 1.40
Nuclear Periphery Foci -4.00 -10.58 -8.31 -5.47 3.00 -1.89 -6.68 -4.78 -4.11 -1.64 -2.44 -11.42 -7.78 -6.04 0.42
Nucleolus -3.27 -5.04 -3.87 -2.72 2.34 -3.31 -6.48 -2.97 -0.70 -0.55 -3.53 -6.17 -4.42 -1.50 1.72
Nucleus -2.17 -4.21 -6.05 -2.35 -0.84 -3.00 -5.44 -2.58 0.06 1.13 -3.21 -5.79 -5.03 -0.84 0.31
Peroxisomes -3.62 -4.25 -1.39 3.33 5.50 -5.49 -1.17 3.00 7.97 4.78 -5.52 -1.90 3.19 8.90 7.29
Vacuole -3.77 -12.00 -14.59 -11.44 -5.66 -5.37 -22.37 -13.53 -10.39 -0.17 -7.23 -30.44 -24.73 -18.01 -4.63
Vacuole Periphery 1.33 -2.04 -3.55 -6.84 -2.74 -2.31 -9.65 -7.55 -6.31 -1.15 -2.48 -13.59 -11.93 -10.08 -2.88