Standard name
Description Poly(A) polymerase; one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0 0 0.05 0 0 0.07 0.05 0.16 0.05 0.08 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.06 0 0.13 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.91 0.98 0.92 0.9 0.83 0.88 0.81 0.78 0.75 0.76 0.88 0.89 0.78 0.88 0.87 0.86 0.9 0.87 0.92 0.84 0.84 0.81
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.21 0.07 0 0.08 0.05 0.17 0.13 0.3 0.28 0.44 0.36 0.08 0.1 0.06 0.08 0.08 0.13 0 0 0 0.05 0.08 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 1 2 2 1 0 1 0 0 0 0 1 2 0 0 0 0
Bud Neck 0 0 0 1 0 0 0 0 2 0 2 0 1 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2 4 6 7 13 1 1 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 6 2 6 9 12 15 5 14 3 6 9 8 29 11 15 8 0 0 0 0 1 1
Endoplasmic Reticulum 0 2 0 0 0 0 0 0 0 0 0 0 0 0 3 2 2 0 1 0 0 0 0
Endosome 0 0 0 2 1 0 2 0 0 0 0 0 1 1 0 1 0 1 1 0 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 1 0
Mitochondria 4 1 0 0 0 5 6 16 9 43 15 1 0 0 1 0 5 0 0 0 0 0 1
Nucleus 123 195 189 145 197 200 328 222 242 250 288 108 150 144 201 170 144 130 199 184 49 99 133
Nuclear Periphery 0 1 0 0 2 4 5 1 1 3 4 0 0 2 1 1 0 0 0 0 0 0 0
Nucleolus 27 14 6 13 12 41 49 83 88 146 137 10 16 12 18 15 22 5 9 8 3 9 11
Peroxisomes 0 0 0 0 1 2 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 2 1 0 0 0 3 3 7 6 1 3 0 0 1 0 0 1 0
Vac/Vac Membrane 0 9 0 3 2 2 1 1 1 3 3 1 1 1 5 5 3 2 7 2 1 2 9
Unique Cell Count 131 215 192 157 219 241 373 273 310 335 380 123 168 185 228 196 168 145 228 201 59 119 164
Labelled Cell Count 156 228 197 170 224 268 410 333 365 457 473 133 186 196 241 212 184 145 228 201 59 119 164


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 11.4 9.8 9.0 9.9 8.7 8.3 7.8 8.2 7.4 7.6 9.3 11.2 10.2 13.3 13.7 13.2 7.5 9.3 10.1
Std Deviation (1e-4) 1.6 3.2 1.6 1.8 2.6 2.3 1.7 1.9 2.2 2.0 2.2 2.1 10.3 2.7 4.2 5.2 4.5 1.4 1.7 2.0
Intensity Change (Log2) -0.12 0.01 -0.17 -0.24 -0.32 -0.26 -0.4 -0.36 -0.07 0.19 0.06 0.44 0.48 0.43 -0.39 -0.07 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.9 2.3 2.0 0 2.2 0 0 0 0 5.2 2.2 3.2 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 3.4 0 5.2 2.8 0 0 0 0 0 0
Nucleus -2.8 -3.6 -5.3 -4.2 -5.7 -6.4 -7.1 -6.9 -4.0 -3.7 -6.2 -4.1 -4.4 -4.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.1 1.2 4.6 3.8 7.4 7.1 9.9 8.6 2.0 2.5 1.5 2.1 2.0 3.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0