Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the EGO/GSE complex; the vacuolar/endosomal membrane associated EGO/GSE complex regulates exit from rapamycin-induced growth arrest, stimulating microautophagy and sorting of Gap1p from the endosome to the plasma membrane; loss results in a defect in vacuolar acidification |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 3 | – | 4 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 1 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 3 | 2 | 2 | 13 | – | 17 | 19 | 4 | 7 | 0 | 1 | 0 | 3 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 27 | 11 | 17 | 26 | 37 | 57 | 75 | – | 66 | 85 | 50 | 55 | 6 | 14 | 15 | 9 | 8 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 3 | 2 | 0 | 1 | 1 | 16 | 21 | – | 23 | 47 | 14 | 12 | 1 | 2 | 2 | 5 | 7 | 7 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 6 | 2 | 13 | 5 | 6 | 2 | 9 | – | 0 | 4 | 1 | 2 | 25 | 25 | 23 | 8 | 7 | 5 | 13 | 13 | 15 | 19 | 32 | 37 |
Golgi | 4 | 2 | 7 | 15 | 56 | 13 | 69 | – | 16 | 22 | 10 | 11 | 2 | 6 | 3 | 22 | 31 | 21 | 1 | 0 | 0 | 2 | 0 | 0 |
Mitochondria | 16 | 19 | 7 | 29 | 8 | 75 | 28 | – | 67 | 50 | 37 | 47 | 5 | 2 | 3 | 4 | 6 | 6 | 8 | 4 | 5 | 5 | 12 | 9 |
Nucleus | 20 | 13 | 14 | 1 | 15 | 23 | 7 | – | 10 | 7 | 6 | 7 | 14 | 19 | 19 | 12 | 10 | 8 | 9 | 5 | 3 | 14 | 12 | 20 |
Nuclear Periphery | 33 | 18 | 22 | 26 | 12 | 30 | 73 | – | 31 | 28 | 10 | 11 | 44 | 84 | 59 | 67 | 50 | 32 | 27 | 9 | 30 | 10 | 46 | 31 |
Nucleolus | 22 | 20 | 17 | 8 | 0 | 5 | 0 | – | 2 | 1 | 1 | 0 | 35 | 29 | 41 | 12 | 12 | 11 | 9 | 2 | 4 | 7 | 12 | 23 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 2 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 0 | 2 | 2 | 2 | 0 | 0 | 1 | 2 |
Vac/Vac Membrane | 287 | 226 | 275 | 260 | 284 | 201 | 390 | – | 185 | 172 | 80 | 91 | 325 | 512 | 527 | 249 | 273 | 196 | 332 | 250 | 284 | 245 | 432 | 365 |
Unique Cell Count | 364 | 272 | 326 | 304 | 356 | 336 | 570 | 338 | 362 | 177 | 203 | 383 | 584 | 605 | 280 | 300 | 222 | 410 | 292 | 352 | 307 | 557 | 501 | |
Labelled Cell Count | 418 | 316 | 373 | 375 | 421 | 424 | 689 | 422 | 438 | 214 | 243 | 458 | 696 | 693 | 393 | 409 | 300 | 410 | 292 | 352 | 307 | 557 | 501 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 14.3 | 14.3 | 12.7 | 12.3 | 12.8 | 8.7 | 11.3 | – | 10.3 | 10.5 | 9.8 | 10.0 | 15.9 | 16.7 | 16.7 | 17.6 | 18.0 | 16.1 | 13.5 | 14.2 | 15.3 |
Std Deviation (1e-4) | 4.3 | 3.7 | 2.7 | 2.5 | 2.7 | 2.4 | 2.3 | – | 2.2 | 2.1 | 2.2 | 2.3 | 3.5 | 3.7 | 3.7 | 5.1 | 4.4 | 4.5 | 3.6 | 3.8 | 3.8 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.01 | -0.55 | -0.17 | – | -0.3 | -0.29 | -0.38 | -0.36 | 0.32 | 0.39 | 0.39 | 0.46 | 0.5 | 0.34 | 0.08 | 0.15 | 0.26 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 4.1 | 4.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.7 | 2.5 | 4.8 | 3.8 | – | 5.6 | 6.7 | 7.3 | 7.1 | -2.7 | -2.2 | -2.2 | -1.2 | -1.6 | -0.6 |
Endoplasmic Reticulum | 0 | 0 | 4.0 | 3.5 | – | 4.8 | 6.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -1.8 | -1.8 | -2.9 | -2.2 | – | -3.7 | -2.4 | 0 | -2.0 | 1.5 | 0.2 | -0.1 | -0.8 | -1.2 | -1.1 |
Golgi | 1.9 | 6.1 | 1.3 | 5.1 | – | 1.8 | 2.6 | 2.1 | 2.0 | 0 | 0 | 0 | 3.3 | 4.3 | 3.8 |
Mitochondria | 4.0 | 0.1 | 7.9 | 2.1 | – | 7.2 | 5.5 | 7.1 | 7.8 | -0.9 | 0 | 0 | -0.7 | -0.1 | 0.4 |
Nucleus | -3.3 | -0.1 | 1.4 | -2.9 | – | -0.9 | -1.8 | -0.5 | -0.5 | -0.4 | -0.8 | -0.9 | 0 | -0.6 | -0.4 |
Nuclear Periphery | 0.9 | -2.0 | 1.0 | 2.8 | – | 1.2 | 0.5 | -0.5 | -0.6 | 2.2 | 3.4 | 1.6 | 6.0 | 3.9 | 3.0 |
Nucleolus | -1.7 | -4.4 | -2.7 | -5.5 | – | -3.6 | -4.1 | -2.7 | -3.3 | 2.0 | -0.2 | 0.9 | -0.5 | -0.7 | -0.1 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.4 | -1.6 | -7.0 | -5.2 | – | -8.3 | -10.1 | -9.2 | -9.6 | 0.2 | 1.4 | 1.2 | 1.6 | 2.5 | 1.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.2472 | 7.5658 | 7.0461 | 6.5452 | 6.9037 | 6.8825 | 8.5973 | 9.5614 | 8.9335 | 8.613 | 8.0983 | 8.8021 | 10.7946 | 12.4098 | 10.8584 | 10.0785 | 10.3589 | 10.9454 |
Actin | 0.0078 | 0 | 0.0047 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0011 | 0 | 0.0048 | 0.0353 | 0 | 0.0038 |
Bud | 0.0001 | 0.0001 | 0.0014 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Bud Neck | 0.0002 | 0.0001 | 0.0001 | 0.0032 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0.0003 | 0 | 0.0001 |
Bud Periphery | 0.0003 | 0.0003 | 0.0024 | 0.0009 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0011 | 0.0005 | 0.0006 | 0.0004 | 0.0002 | 0.0002 |
Bud Site | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0004 | 0 | 0 |
Cell Periphery | 0.0005 | 0.0008 | 0.0007 | 0.0008 | 0.0004 | 0.0002 | 0.0005 | 0.0004 | 0.0005 | 0.0004 | 0.0001 | 0.0002 | 0.0007 | 0.0004 | 0.0006 | 0.0002 | 0.0002 | 0.0002 |
Cytoplasm | 0.001 | 0.0008 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0014 | 0.0008 | 0.0002 | 0.0001 | 0.0003 |
Cytoplasmic Foci | 0.0056 | 0.0013 | 0.0014 | 0.0002 | 0.0001 | 0.0002 | 0.0033 | 0.0028 | 0.0043 | 0.0002 | 0.0009 | 0.0001 | 0.001 | 0.0005 | 0.0028 | 0.0049 | 0.0002 | 0.0006 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.001 | 0.0005 | 0.0011 | 0.0005 | 0.0022 | 0.0003 | 0.0006 | 0.001 | 0.0003 | 0.0017 | 0.0003 | 0.0002 | 0.0011 | 0.0008 | 0.0009 | 0.0004 | 0.0002 | 0.0004 |
Endosome | 0.0305 | 0.0174 | 0.0229 | 0.0172 | 0.0111 | 0.0117 | 0.0205 | 0.014 | 0.0293 | 0.013 | 0.0362 | 0.0126 | 0.0341 | 0.0192 | 0.0228 | 0.0076 | 0.0213 | 0.0147 |
Golgi | 0.0024 | 0.0005 | 0.0032 | 0.0013 | 0.0001 | 0.0003 | 0.001 | 0.0003 | 0.0019 | 0.0006 | 0.0021 | 0.0003 | 0.0044 | 0.0004 | 0.0062 | 0.0055 | 0.0005 | 0.0006 |
Lipid Particles | 0.0043 | 0.0026 | 0.0074 | 0.0014 | 0.0003 | 0.002 | 0.0096 | 0.0021 | 0.0057 | 0.0028 | 0.0025 | 0.0013 | 0.0026 | 0.0025 | 0.0029 | 0.0088 | 0.0018 | 0.0041 |
Mitochondria | 0.0112 | 0.0039 | 0.0184 | 0.0069 | 0.0026 | 0.0014 | 0.0048 | 0.0015 | 0.0069 | 0.0006 | 0.0012 | 0.0016 | 0.0197 | 0.001 | 0.0093 | 0.0574 | 0.0022 | 0.0037 |
None | 0.0016 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0011 | 0.0003 | 0 | 0.0003 |
Nuclear Periphery | 0.0056 | 0.0095 | 0.0084 | 0.0033 | 0.0059 | 0.0192 | 0.0094 | 0.0167 | 0.0077 | 0.0226 | 0.0044 | 0.0103 | 0.0086 | 0.0127 | 0.0182 | 0.0075 | 0.0043 | 0.0123 |
Nucleolus | 0.001 | 0.0091 | 0.0058 | 0.0021 | 0.0001 | 0.0006 | 0.0005 | 0.0012 | 0.0011 | 0.0002 | 0.0001 | 0.0004 | 0.001 | 0.0004 | 0.0007 | 0.0002 | 0.0002 | 0.0031 |
Nucleus | 0.0035 | 0.0019 | 0.006 | 0.0024 | 0.0007 | 0.0006 | 0.001 | 0.0012 | 0.0006 | 0.0006 | 0.0003 | 0.0005 | 0.0014 | 0.0006 | 0.0019 | 0.0004 | 0.0004 | 0.0011 |
Peroxisomes | 0.001 | 0.0001 | 0.0006 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0012 | 0.0074 | 0 | 0.0001 |
Punctate Nuclear | 0.0012 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0005 | 0.0014 | 0 | 0.0003 |
Vacuole | 0.3036 | 0.2656 | 0.2938 | 0.2525 | 0.2525 | 0.2136 | 0.2694 | 0.2375 | 0.2865 | 0.2986 | 0.2195 | 0.201 | 0.2701 | 0.2646 | 0.2344 | 0.2101 | 0.2133 | 0.1716 |
Vacuole Periphery | 0.6171 | 0.6854 | 0.6207 | 0.7062 | 0.7234 | 0.7496 | 0.6784 | 0.7204 | 0.6534 | 0.6581 | 0.7317 | 0.7709 | 0.6509 | 0.6948 | 0.6895 | 0.6512 | 0.7547 | 0.7824 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 24.8515 | 10.6635 | 23.7384 | 28.8463 | 37.3764 | 17.8831 | 25.607 | 30.9274 | 34.187 | 31.7095 |
Translational Efficiency | 1.1538 | 2.2772 | 1.0607 | 0.8811 | 0.7427 | 1.8233 | 1.062 | 1.2688 | 1.1014 | 1.2648 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1770 | 1645 | 3077 | 123 | 1130 | 2479 | 1666 | 1645 | 2900 | 4124 | 4743 | 1768 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1156.76 | 1164.25 | 1529.68 | 1560.12 | 1111.58 | 1247.84 | 1478.71 | 1464.95 | 1139.16 | 1214.50 | 1511.78 | 1471.57 |
Standard Deviation | 172.83 | 226.84 | 256.46 | 249.19 | 201.20 | 222.75 | 230.14 | 245.44 | 185.71 | 228.09 | 248.73 | 246.89 |
Intensity Change Log 2 | — | 0.009311 | 0.403140 | 0.431567 | — | 0.166821 | 0.411727 | 0.398240 | — | 0.088647 | 0.407355 | 0.415332 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000013 | 0.000139 | 0.000024 | 0.000381 | 0.000063 | 0.000097 | 0.000053 | 0.000027 | 0.000032 | 0.000114 | 0.000034 | 0.000052 |
Bud Neck | 0.000154 | 0.000899 | 0.000627 | 0.006048 | 0.000549 | 0.002731 | 0.001138 | 0.000752 | 0.000308 | 0.002000 | 0.000806 | 0.001121 |
Bud Site | 0.001113 | 0.005339 | 0.000279 | 0.007668 | 0.002723 | 0.003066 | 0.000714 | 0.001397 | 0.001740 | 0.003973 | 0.000432 | 0.001833 |
Cell Periphery | 0.000867 | 0.001065 | 0.000492 | 0.000281 | 0.000401 | 0.000746 | 0.000298 | 0.000992 | 0.000685 | 0.000873 | 0.000424 | 0.000943 |
Cytoplasm | 0.000296 | 0.002909 | 0.000041 | 0.000244 | 0.001981 | 0.000824 | 0.000048 | 0.000167 | 0.000953 | 0.001655 | 0.000043 | 0.000172 |
Cytoplasmic Foci | 0.000327 | 0.008064 | 0.000703 | 0.003475 | 0.002187 | 0.004470 | 0.001129 | 0.001132 | 0.001052 | 0.005904 | 0.000853 | 0.001295 |
Eisosomes | 0.000003 | 0.000016 | 0.000091 | 0.000038 | 0.000006 | 0.000054 | 0.000046 | 0.000025 | 0.000004 | 0.000039 | 0.000075 | 0.000026 |
Endoplasmic Reticulum | 0.000581 | 0.000740 | 0.000229 | 0.000247 | 0.000625 | 0.000858 | 0.000234 | 0.000325 | 0.000598 | 0.000811 | 0.000231 | 0.000319 |
Endosome | 0.001872 | 0.018819 | 0.000386 | 0.004760 | 0.006149 | 0.010503 | 0.001092 | 0.002217 | 0.003539 | 0.013820 | 0.000634 | 0.002394 |
Golgi | 0.000915 | 0.014898 | 0.000231 | 0.021991 | 0.002793 | 0.002989 | 0.001155 | 0.004915 | 0.001647 | 0.007739 | 0.000556 | 0.006103 |
Lipid Particles | 0.000171 | 0.002104 | 0.001205 | 0.000955 | 0.000314 | 0.003461 | 0.000911 | 0.000739 | 0.000226 | 0.002920 | 0.001102 | 0.000754 |
Mitochondria | 0.003724 | 0.015394 | 0.003581 | 0.012307 | 0.006928 | 0.023952 | 0.006185 | 0.007614 | 0.004972 | 0.020538 | 0.004496 | 0.007941 |
Mitotic Spindle | 0.000062 | 0.000531 | 0.000068 | 0.001125 | 0.000581 | 0.000977 | 0.000259 | 0.000626 | 0.000264 | 0.000800 | 0.000135 | 0.000660 |
None | 0.000082 | 0.000919 | 0.000296 | 0.000269 | 0.000191 | 0.000915 | 0.000296 | 0.000264 | 0.000124 | 0.000917 | 0.000296 | 0.000264 |
Nuclear Periphery | 0.000397 | 0.000703 | 0.002236 | 0.005261 | 0.000684 | 0.004188 | 0.001894 | 0.000791 | 0.000509 | 0.002798 | 0.002116 | 0.001102 |
Nuclear Periphery Foci | 0.000197 | 0.001676 | 0.000894 | 0.001822 | 0.000532 | 0.005066 | 0.001132 | 0.000571 | 0.000327 | 0.003714 | 0.000978 | 0.000658 |
Nucleolus | 0.000345 | 0.002265 | 0.006647 | 0.003545 | 0.001231 | 0.009485 | 0.012242 | 0.002564 | 0.000690 | 0.006605 | 0.008612 | 0.002632 |
Nucleus | 0.000297 | 0.000892 | 0.003365 | 0.001555 | 0.001454 | 0.003930 | 0.003038 | 0.000972 | 0.000748 | 0.002718 | 0.003250 | 0.001013 |
Peroxisomes | 0.000060 | 0.001352 | 0.000149 | 0.002084 | 0.000426 | 0.000977 | 0.000258 | 0.000112 | 0.000202 | 0.001126 | 0.000187 | 0.000249 |
Vacuole | 0.146144 | 0.236314* | 0.247835* | 0.341561* | 0.168483 | 0.268130* | 0.347994* | 0.363171* | 0.154848 | 0.255439* | 0.283016* | 0.361667* |
Vacuole Periphery | 0.842382* | 0.684963* | 0.730622* | 0.584384* | 0.801700* | 0.652581* | 0.619886* | 0.610626* | 0.826530* | 0.665497* | 0.691726* | 0.608801* |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.96 | -2.82 | -1.42 | -0.94 | -1.38 | -2.02 | 0.31 | 2.40 | 6.30 | 2.74 | -5.39 | -0.47 | -1.02 | 2.62 | -0.88 |
Bud Neck | -7.60 | -6.00 | -2.27 | -1.99 | -2.09 | -7.50 | -3.53 | -1.29 | 7.25 | 2.91 | -9.90 | -6.12 | -3.90 | 3.43 | -1.46 |
Bud Site | -5.88 | 2.42 | -1.85 | -0.65 | -2.09 | -0.54 | 3.23 | 1.87 | 3.25 | -1.97 | -4.84 | 4.08 | -0.27 | 4.04 | -3.57 |
Cell Periphery | -0.63 | 1.25 | 1.96 | 6.22 | 2.78 | -3.20 | 1.86 | -3.90 | -1.42 | -4.72 | -0.96 | 1.42 | -1.14 | -0.45 | -3.73 |
Cytoplasm | -3.39 | 1.37 | 0.24 | 3.54 | -2.21 | 1.52 | 2.71 | 2.53 | 2.43 | -1.88 | -1.55 | 3.02 | 2.54 | 4.32 | -2.20 |
Cytoplasmic Foci | -7.61 | -1.70 | -1.77 | 2.25 | -1.54 | -3.14 | 2.00 | 1.72 | 4.94 | -0.01 | -8.63 | 0.77 | -0.60 | 7.16 | -1.09 |
Eisosomes | -5.92 | -7.99 | -2.61 | -1.60 | 3.04 | -8.78 | -8.78 | -5.86 | 4.78 | 3.96 | -10.28 | -9.67 | -7.25 | 2.97 | 6.26 |
Endoplasmic Reticulum | -1.09 | 2.85 | 2.24 | 4.17 | -0.21 | -2.11 | 3.87 | 3.03 | 9.38 | -2.69 | -2.24 | 4.31 | 3.22 | 9.74 | -3.55 |
Endosome | -10.42 | 4.57 | -2.60 | 7.29 | -4.08 | -3.64 | 5.07 | 3.94 | 11.55 | -4.35 | -11.75 | 6.65 | 2.42 | 14.54 | -8.70 |
Golgi | -11.24 | 4.65 | -2.40 | -0.79 | -2.47 | -0.40 | 3.24 | -2.16 | -2.15 | -4.29 | -10.91 | 5.07 | -4.32 | 1.46 | -5.48 |
Lipid Particles | -4.37 | -17.64 | -1.96 | 1.90 | 0.58 | -9.39 | -4.91 | -4.37 | 8.35 | 1.82 | -10.15 | -14.86 | -8.62 | 8.11 | 5.15 |
Mitochondria | -6.55 | 0.15 | -2.19 | 0.73 | -2.24 | -7.80 | 0.48 | -0.42 | 7.82 | -1.02 | -10.96 | 0.59 | -2.43 | 7.74 | -3.06 |
Mitotic Spindle | -5.87 | -0.18 | -1.91 | -1.05 | -1.90 | -0.85 | 0.59 | -0.07 | 0.62 | -0.53 | -2.35 | 0.63 | -0.77 | 0.29 | -1.01 |
None | -4.84 | -15.78 | -3.86 | 3.62 | 0.55 | -7.33 | -3.82 | -2.80 | 6.70 | 1.51 | -8.75 | -12.64 | -8.71 | 7.17 | 1.93 |
Nuclear Periphery | -2.49 | -5.45 | -2.82 | -2.64 | -1.72 | -7.79 | -3.37 | -0.52 | 8.15 | 3.48 | -8.70 | -6.32 | -3.55 | 5.85 | 3.60 |
Nuclear Periphery Foci | -4.27 | -6.20 | -3.65 | -0.11 | -2.04 | -8.39 | -3.04 | -0.29 | 8.44 | 3.20 | -9.66 | -6.36 | -3.90 | 8.66 | 2.86 |
Nucleolus | -3.96 | -12.24 | -3.39 | -1.21 | 2.81 | -7.85 | -8.20 | -2.14 | 6.96 | 7.45 | -9.49 | -13.53 | -4.83 | 5.81 | 9.16 |
Nucleus | -3.21 | -7.58 | -2.26 | -1.17 | 2.57 | -3.30 | -2.46 | 0.81 | 5.93 | 6.20 | -5.25 | -6.93 | -1.00 | 5.23 | 7.24 |
Peroxisomes | -3.87 | -3.01 | -1.13 | -0.40 | -1.08 | -2.88 | 1.01 | 1.99 | 7.32 | 3.85 | -5.72 | 0.18 | -0.34 | 4.50 | -0.47 |
Vacuole | -15.35 | -18.95 | -9.56 | -5.12 | -4.59 | -15.62 | -24.63 | -26.02 | -13.84 | -1.98 | -23.65 | -29.23 | -33.54 | -17.21 | -12.57 |
Vacuole Periphery | 20.53* | 19.09 | 10.70 | 4.09 | 6.08 | 18.19* | 21.48 | 22.18 | 5.18 | 1.10 | 29.68 | 27.37 | 32.13 | 7.92 | 12.32 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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