Standard name
Human Ortholog
Description Subunit of the EGO/GSE complex; the vacuolar/endosomal membrane associated EGO/GSE complex regulates exit from rapamycin-induced growth arrest, stimulating microautophagy and sorting of Gap1p from the endosome to the plasma membrane; loss results in a defect in vacuolar acidification

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0 0.05 0.09 0.1 0.17 0.13 0.2 0.23 0.28 0.27 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0.05 0 0.07 0.13 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0.06 0.06 0.07
Golgi 0 0 0 0.05 0.16 0 0.12 0.05 0.06 0.06 0.05 0 0 0 0.08 0.1 0.09 0 0 0 0 0 0
Mitochondria 0 0.07 0 0.1 0 0.22 0.05 0.2 0.14 0.21 0.23 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.05 0.05 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Nuclear Periphery 0.09 0.07 0.07 0.09 0 0.09 0.13 0.09 0.08 0.06 0.05 0.11 0.14 0.1 0.24 0.17 0.14 0.07 0 0.09 0 0.08 0.06
Nucleolus 0.06 0.07 0.05 0 0 0 0 0 0 0 0 0.09 0.05 0.07 0 0 0.05 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.79 0.83 0.84 0.86 0.8 0.6 0.68 0.55 0.48 0.45 0.45 0.85 0.88 0.87 0.89 0.91 0.88 0.81 0.86 0.81 0.8 0.78 0.73
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 3 4 3 1 0 0 0 0 0 0 1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 2 1 2 0 1 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 3 2 2 13 17 19 4 7 0 1 0 3 5 1 0 0 0 0 0 0
Cytoplasm 27 11 17 26 37 57 75 66 85 50 55 6 14 15 9 8 9 0 0 0 0 0 0
Endoplasmic Reticulum 3 2 0 1 1 16 21 23 47 14 12 1 2 2 5 7 7 0 0 0 0 1 1
Endosome 6 2 13 5 6 2 9 0 4 1 2 25 25 23 8 7 5 13 13 15 19 32 37
Golgi 4 2 7 15 56 13 69 16 22 10 11 2 6 3 22 31 21 1 0 0 2 0 0
Mitochondria 16 19 7 29 8 75 28 67 50 37 47 5 2 3 4 6 6 8 4 5 5 12 9
Nucleus 20 13 14 1 15 23 7 10 7 6 7 14 19 19 12 10 8 9 5 3 14 12 20
Nuclear Periphery 33 18 22 26 12 30 73 31 28 10 11 44 84 59 67 50 32 27 9 30 10 46 31
Nucleolus 22 20 17 8 0 5 0 2 1 1 0 35 29 41 12 12 11 9 2 4 7 12 23
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 2 1 1 0 0 0 0 0 0 0 1 2 1 2 0 2 2 2 0 0 1 2
Vac/Vac Membrane 287 226 275 260 284 201 390 185 172 80 91 325 512 527 249 273 196 332 250 284 245 432 365
Unique Cell Count 364 272 326 304 356 336 570 338 362 177 203 383 584 605 280 300 222 410 292 352 307 557 501
Labelled Cell Count 418 316 373 375 421 424 689 422 438 214 243 458 696 693 393 409 300 410 292 352 307 557 501


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.3 14.3 12.7 12.3 12.8 8.7 11.3 10.3 10.5 9.8 10.0 15.9 16.7 16.7 17.6 18.0 16.1 13.5 14.2 15.3
Std Deviation (1e-4) 4.3 3.7 2.7 2.5 2.7 2.4 2.3 2.2 2.1 2.2 2.3 3.5 3.7 3.7 5.1 4.4 4.5 3.6 3.8 3.8
Intensity Change (Log2) -0.05 0.01 -0.55 -0.17 -0.3 -0.29 -0.38 -0.36 0.32 0.39 0.39 0.46 0.5 0.34 0.08 0.15 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 4.1 4.2 0 0 0 0 0 0 0 0
Cytoplasm 1.7 2.5 4.8 3.8 5.6 6.7 7.3 7.1 -2.7 -2.2 -2.2 -1.2 -1.6 -0.6
Endoplasmic Reticulum 0 0 4.0 3.5 4.8 6.7 0 0 0 0 0 0 0 0
Endosome -1.8 -1.8 -2.9 -2.2 -3.7 -2.4 0 -2.0 1.5 0.2 -0.1 -0.8 -1.2 -1.1
Golgi 1.9 6.1 1.3 5.1 1.8 2.6 2.1 2.0 0 0 0 3.3 4.3 3.8
Mitochondria 4.0 0.1 7.9 2.1 7.2 5.5 7.1 7.8 -0.9 0 0 -0.7 -0.1 0.4
Nucleus -3.3 -0.1 1.4 -2.9 -0.9 -1.8 -0.5 -0.5 -0.4 -0.8 -0.9 0 -0.6 -0.4
Nuclear Periphery 0.9 -2.0 1.0 2.8 1.2 0.5 -0.5 -0.6 2.2 3.4 1.6 6.0 3.9 3.0
Nucleolus -1.7 -4.4 -2.7 -5.5 -3.6 -4.1 -2.7 -3.3 2.0 -0.2 0.9 -0.5 -0.7 -0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.4 -1.6 -7.0 -5.2 -8.3 -10.1 -9.2 -9.6 0.2 1.4 1.2 1.6 2.5 1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.2472 7.5658 7.0461 6.5452 6.9037 6.8825 8.5973 9.5614 8.9335 8.613 8.0983 8.8021 10.7946 12.4098 10.8584 10.0785 10.3589 10.9454
Actin 0.0078 0 0.0047 0.0001 0 0 0 0 0 0.0001 0 0 0.0011 0 0.0048 0.0353 0 0.0038
Bud 0.0001 0.0001 0.0014 0.0002 0 0 0 0.0001 0.0002 0.0001 0 0.0001 0.0003 0.0001 0.0002 0.0002 0.0001 0.0001
Bud Neck 0.0002 0.0001 0.0001 0.0032 0 0 0 0 0.0001 0 0 0 0.0002 0 0.0002 0.0003 0 0.0001
Bud Periphery 0.0003 0.0003 0.0024 0.0009 0.0003 0.0002 0.0001 0.0001 0.0004 0.0001 0.0002 0.0002 0.0011 0.0005 0.0006 0.0004 0.0002 0.0002
Bud Site 0.0002 0.0001 0.0002 0.0005 0 0 0 0.0001 0.0003 0 0 0.0001 0.0002 0 0.0003 0.0004 0 0
Cell Periphery 0.0005 0.0008 0.0007 0.0008 0.0004 0.0002 0.0005 0.0004 0.0005 0.0004 0.0001 0.0002 0.0007 0.0004 0.0006 0.0002 0.0002 0.0002
Cytoplasm 0.001 0.0008 0.0004 0.0002 0.0001 0.0001 0.0002 0.0004 0.0003 0.0002 0.0002 0.0002 0.0005 0.0014 0.0008 0.0002 0.0001 0.0003
Cytoplasmic Foci 0.0056 0.0013 0.0014 0.0002 0.0001 0.0002 0.0033 0.0028 0.0043 0.0002 0.0009 0.0001 0.001 0.0005 0.0028 0.0049 0.0002 0.0006
Eisosomes 0.0001 0 0 0 0 0 0 0 0 0 0 0 0.0001 0 0 0.0002 0 0
Endoplasmic Reticulum 0.001 0.0005 0.0011 0.0005 0.0022 0.0003 0.0006 0.001 0.0003 0.0017 0.0003 0.0002 0.0011 0.0008 0.0009 0.0004 0.0002 0.0004
Endosome 0.0305 0.0174 0.0229 0.0172 0.0111 0.0117 0.0205 0.014 0.0293 0.013 0.0362 0.0126 0.0341 0.0192 0.0228 0.0076 0.0213 0.0147
Golgi 0.0024 0.0005 0.0032 0.0013 0.0001 0.0003 0.001 0.0003 0.0019 0.0006 0.0021 0.0003 0.0044 0.0004 0.0062 0.0055 0.0005 0.0006
Lipid Particles 0.0043 0.0026 0.0074 0.0014 0.0003 0.002 0.0096 0.0021 0.0057 0.0028 0.0025 0.0013 0.0026 0.0025 0.0029 0.0088 0.0018 0.0041
Mitochondria 0.0112 0.0039 0.0184 0.0069 0.0026 0.0014 0.0048 0.0015 0.0069 0.0006 0.0012 0.0016 0.0197 0.001 0.0093 0.0574 0.0022 0.0037
None 0.0016 0 0.0001 0.0001 0 0 0.0001 0 0 0 0 0 0.0004 0 0.0011 0.0003 0 0.0003
Nuclear Periphery 0.0056 0.0095 0.0084 0.0033 0.0059 0.0192 0.0094 0.0167 0.0077 0.0226 0.0044 0.0103 0.0086 0.0127 0.0182 0.0075 0.0043 0.0123
Nucleolus 0.001 0.0091 0.0058 0.0021 0.0001 0.0006 0.0005 0.0012 0.0011 0.0002 0.0001 0.0004 0.001 0.0004 0.0007 0.0002 0.0002 0.0031
Nucleus 0.0035 0.0019 0.006 0.0024 0.0007 0.0006 0.001 0.0012 0.0006 0.0006 0.0003 0.0005 0.0014 0.0006 0.0019 0.0004 0.0004 0.0011
Peroxisomes 0.001 0.0001 0.0006 0.0001 0 0 0.0002 0 0.0005 0 0.0001 0 0.0003 0 0.0012 0.0074 0 0.0001
Punctate Nuclear 0.0012 0 0.0001 0 0 0 0.0002 0 0 0.0001 0 0 0.0002 0 0.0005 0.0014 0 0.0003
Vacuole 0.3036 0.2656 0.2938 0.2525 0.2525 0.2136 0.2694 0.2375 0.2865 0.2986 0.2195 0.201 0.2701 0.2646 0.2344 0.2101 0.2133 0.1716
Vacuole Periphery 0.6171 0.6854 0.6207 0.7062 0.7234 0.7496 0.6784 0.7204 0.6534 0.6581 0.7317 0.7709 0.6509 0.6948 0.6895 0.6512 0.7547 0.7824

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.8515 10.6635 23.7384 28.8463 37.3764 17.8831 25.607 30.9274 34.187 31.7095
Translational Efficiency 1.1538 2.2772 1.0607 0.8811 0.7427 1.8233 1.062 1.2688 1.1014 1.2648

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1770 1645 3077 123 1130 2479 1666 1645 2900 4124 4743 1768

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1156.76 1164.25 1529.68 1560.12 1111.58 1247.84 1478.71 1464.95 1139.16 1214.50 1511.78 1471.57
Standard Deviation 172.83 226.84 256.46 249.19 201.20 222.75 230.14 245.44 185.71 228.09 248.73 246.89
Intensity Change Log 2 0.009311 0.403140 0.431567 0.166821 0.411727 0.398240 0.088647 0.407355 0.415332

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000013 0.000139 0.000024 0.000381 0.000063 0.000097 0.000053 0.000027 0.000032 0.000114 0.000034 0.000052
Bud Neck 0.000154 0.000899 0.000627 0.006048 0.000549 0.002731 0.001138 0.000752 0.000308 0.002000 0.000806 0.001121
Bud Site 0.001113 0.005339 0.000279 0.007668 0.002723 0.003066 0.000714 0.001397 0.001740 0.003973 0.000432 0.001833
Cell Periphery 0.000867 0.001065 0.000492 0.000281 0.000401 0.000746 0.000298 0.000992 0.000685 0.000873 0.000424 0.000943
Cytoplasm 0.000296 0.002909 0.000041 0.000244 0.001981 0.000824 0.000048 0.000167 0.000953 0.001655 0.000043 0.000172
Cytoplasmic Foci 0.000327 0.008064 0.000703 0.003475 0.002187 0.004470 0.001129 0.001132 0.001052 0.005904 0.000853 0.001295
Eisosomes 0.000003 0.000016 0.000091 0.000038 0.000006 0.000054 0.000046 0.000025 0.000004 0.000039 0.000075 0.000026
Endoplasmic Reticulum 0.000581 0.000740 0.000229 0.000247 0.000625 0.000858 0.000234 0.000325 0.000598 0.000811 0.000231 0.000319
Endosome 0.001872 0.018819 0.000386 0.004760 0.006149 0.010503 0.001092 0.002217 0.003539 0.013820 0.000634 0.002394
Golgi 0.000915 0.014898 0.000231 0.021991 0.002793 0.002989 0.001155 0.004915 0.001647 0.007739 0.000556 0.006103
Lipid Particles 0.000171 0.002104 0.001205 0.000955 0.000314 0.003461 0.000911 0.000739 0.000226 0.002920 0.001102 0.000754
Mitochondria 0.003724 0.015394 0.003581 0.012307 0.006928 0.023952 0.006185 0.007614 0.004972 0.020538 0.004496 0.007941
Mitotic Spindle 0.000062 0.000531 0.000068 0.001125 0.000581 0.000977 0.000259 0.000626 0.000264 0.000800 0.000135 0.000660
None 0.000082 0.000919 0.000296 0.000269 0.000191 0.000915 0.000296 0.000264 0.000124 0.000917 0.000296 0.000264
Nuclear Periphery 0.000397 0.000703 0.002236 0.005261 0.000684 0.004188 0.001894 0.000791 0.000509 0.002798 0.002116 0.001102
Nuclear Periphery Foci 0.000197 0.001676 0.000894 0.001822 0.000532 0.005066 0.001132 0.000571 0.000327 0.003714 0.000978 0.000658
Nucleolus 0.000345 0.002265 0.006647 0.003545 0.001231 0.009485 0.012242 0.002564 0.000690 0.006605 0.008612 0.002632
Nucleus 0.000297 0.000892 0.003365 0.001555 0.001454 0.003930 0.003038 0.000972 0.000748 0.002718 0.003250 0.001013
Peroxisomes 0.000060 0.001352 0.000149 0.002084 0.000426 0.000977 0.000258 0.000112 0.000202 0.001126 0.000187 0.000249
Vacuole 0.146144 0.236314* 0.247835* 0.341561* 0.168483 0.268130* 0.347994* 0.363171* 0.154848 0.255439* 0.283016* 0.361667*
Vacuole Periphery 0.842382* 0.684963* 0.730622* 0.584384* 0.801700* 0.652581* 0.619886* 0.610626* 0.826530* 0.665497* 0.691726* 0.608801*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.96 -2.82 -1.42 -0.94 -1.38 -2.02 0.31 2.40 6.30 2.74 -5.39 -0.47 -1.02 2.62 -0.88
Bud Neck -7.60 -6.00 -2.27 -1.99 -2.09 -7.50 -3.53 -1.29 7.25 2.91 -9.90 -6.12 -3.90 3.43 -1.46
Bud Site -5.88 2.42 -1.85 -0.65 -2.09 -0.54 3.23 1.87 3.25 -1.97 -4.84 4.08 -0.27 4.04 -3.57
Cell Periphery -0.63 1.25 1.96 6.22 2.78 -3.20 1.86 -3.90 -1.42 -4.72 -0.96 1.42 -1.14 -0.45 -3.73
Cytoplasm -3.39 1.37 0.24 3.54 -2.21 1.52 2.71 2.53 2.43 -1.88 -1.55 3.02 2.54 4.32 -2.20
Cytoplasmic Foci -7.61 -1.70 -1.77 2.25 -1.54 -3.14 2.00 1.72 4.94 -0.01 -8.63 0.77 -0.60 7.16 -1.09
Eisosomes -5.92 -7.99 -2.61 -1.60 3.04 -8.78 -8.78 -5.86 4.78 3.96 -10.28 -9.67 -7.25 2.97 6.26
Endoplasmic Reticulum -1.09 2.85 2.24 4.17 -0.21 -2.11 3.87 3.03 9.38 -2.69 -2.24 4.31 3.22 9.74 -3.55
Endosome -10.42 4.57 -2.60 7.29 -4.08 -3.64 5.07 3.94 11.55 -4.35 -11.75 6.65 2.42 14.54 -8.70
Golgi -11.24 4.65 -2.40 -0.79 -2.47 -0.40 3.24 -2.16 -2.15 -4.29 -10.91 5.07 -4.32 1.46 -5.48
Lipid Particles -4.37 -17.64 -1.96 1.90 0.58 -9.39 -4.91 -4.37 8.35 1.82 -10.15 -14.86 -8.62 8.11 5.15
Mitochondria -6.55 0.15 -2.19 0.73 -2.24 -7.80 0.48 -0.42 7.82 -1.02 -10.96 0.59 -2.43 7.74 -3.06
Mitotic Spindle -5.87 -0.18 -1.91 -1.05 -1.90 -0.85 0.59 -0.07 0.62 -0.53 -2.35 0.63 -0.77 0.29 -1.01
None -4.84 -15.78 -3.86 3.62 0.55 -7.33 -3.82 -2.80 6.70 1.51 -8.75 -12.64 -8.71 7.17 1.93
Nuclear Periphery -2.49 -5.45 -2.82 -2.64 -1.72 -7.79 -3.37 -0.52 8.15 3.48 -8.70 -6.32 -3.55 5.85 3.60
Nuclear Periphery Foci -4.27 -6.20 -3.65 -0.11 -2.04 -8.39 -3.04 -0.29 8.44 3.20 -9.66 -6.36 -3.90 8.66 2.86
Nucleolus -3.96 -12.24 -3.39 -1.21 2.81 -7.85 -8.20 -2.14 6.96 7.45 -9.49 -13.53 -4.83 5.81 9.16
Nucleus -3.21 -7.58 -2.26 -1.17 2.57 -3.30 -2.46 0.81 5.93 6.20 -5.25 -6.93 -1.00 5.23 7.24
Peroxisomes -3.87 -3.01 -1.13 -0.40 -1.08 -2.88 1.01 1.99 7.32 3.85 -5.72 0.18 -0.34 4.50 -0.47
Vacuole -15.35 -18.95 -9.56 -5.12 -4.59 -15.62 -24.63 -26.02 -13.84 -1.98 -23.65 -29.23 -33.54 -17.21 -12.57
Vacuole Periphery 20.53* 19.09 10.70 4.09 6.08 18.19* 21.48 22.18 5.18 1.10 29.68 27.37 32.13 7.92 12.32