ORF | |
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Human Ortholog | |
Description | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | – | 6 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 3 | 9 | 1 | 6 | 8 | 12 | 6 | 10 | – | 2 | 8 | 3 | 0 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 325 | 245 | 440 | 221 | 270 | 467 | 566 | 420 | 312 | – | 193 | 329 | 247 | 294 | 272 | 49 | 63 | 21 | 317 | 239 | 486 | 71 | 174 | 164 |
Endoplasmic Reticulum | 1 | 3 | 7 | 0 | 12 | 18 | 52 | 34 | 17 | – | 7 | 27 | 3 | 2 | 1 | 2 | 3 | 0 | 1 | 0 | 1 | 0 | 1 | 4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 0 | 0 | 0 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 5 | – | 13 | 10 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 0 |
Nucleus | 2 | 1 | 2 | 2 | 3 | 3 | 18 | 17 | 13 | – | 9 | 11 | 4 | 0 | 3 | 4 | 1 | 0 | 0 | 2 | 3 | 1 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 18 | 9 | 2 | 12 | 11 | 19 | 8 | – | 51 | 83 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 2 | 2 | 1 | 2 | 4 |
Unique Cell Count | 328 | 250 | 466 | 229 | 284 | 500 | 644 | 481 | 350 | 268 | 450 | 252 | 296 | 276 | 53 | 64 | 22 | 326 | 248 | 509 | 77 | 181 | 180 | |
Labelled Cell Count | 335 | 254 | 476 | 233 | 293 | 511 | 661 | 498 | 366 | 282 | 471 | 257 | 296 | 279 | 62 | 68 | 22 | 326 | 248 | 509 | 77 | 181 | 180 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 14.4 | 18.4 | 12.4 | 11.7 | 12.7 | 11.6 | 12.2 | 12.2 | 10.8 | – | 9.2 | 11.2 | 15.9 | 16.9 | 16.6 | 25.2 | 23.6 | 21.0 | 13.6 | 14.0 | 16.3 |
Std Deviation (1e-4) | 2.3 | 3.2 | 1.9 | 2.1 | 1.8 | 1.8 | 1.9 | 2.0 | 2.1 | – | 1.9 | 2.5 | 2.5 | 2.7 | 3.0 | 7.1 | 7.3 | 3.4 | 1.8 | 2.1 | 3.5 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.04 | -0.1 | -0.03 | -0.02 | -0.2 | – | -0.44 | -0.15 | 0.35 | 0.44 | 0.42 | 1.02 | 0.93 | 0.76 | 0.13 | 0.18 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0.2 | -0.4 | -0.1 | -0.8 | 0.9 | – | 0 | -0.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.2 | 0.4 | -0.7 | -3.7 | -3.8 | -2.8 | – | -8.5 | -8.8 | 2.3 | 3.5 | 2.8 | -0.6 | 1.4 | 0.2 |
Endoplasmic Reticulum | 0 | 2.3 | 2.1 | 4.8 | 4.2 | 2.8 | – | 1.1 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 2.9 | 3.4 | 3.5 | – | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | -2.6 | -1.3 | -2.2 | 0.1 | -1.3 | – | 6.8 | 7.0 | -3.2 | -3.4 | -3.3 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.8855 | 9.5334 | 7.5913 | 7.5279 | 7.5036 | 8.1355 | 9.7405 | 12.2972 | 11.4388 | 10.645 | 10.2145 | 11.4373 | 9.521 | 11.116 | 10.1795 | 10.1034 | 9.3956 | 10.3597 |
Actin | 0.0076 | 0.0003 | 0.0017 | 0.0002 | 0.0013 | 0.001 | 0.026 | 0.001 | 0.0064 | 0.007 | 0.0002 | 0.0063 | 0.0142 | 0.001 | 0.0044 | 0.0001 | 0.0022 | 0.0006 |
Bud | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0006 | 0.0003 | 0.0046 | 0.0002 | 0 | 0.0003 | 0.0008 | 0.0004 | 0.0006 | 0 | 0.0001 | 0.0001 |
Bud Neck | 0.0015 | 0.0002 | 0.001 | 0.0002 | 0.0007 | 0.0008 | 0.0021 | 0.0003 | 0.0012 | 0.0007 | 0.0008 | 0.0024 | 0.0017 | 0.0002 | 0.0006 | 0.0003 | 0.0006 | 0.0013 |
Bud Periphery | 0.0001 | 0 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0037 | 0.0001 | 0 | 0.0007 | 0.0009 | 0.0001 | 0.0006 | 0 | 0 | 0 |
Bud Site | 0.0008 | 0.0005 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0028 | 0.002 | 0.003 | 0.0003 | 0 | 0.0004 | 0.0029 | 0.0009 | 0.0023 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.9298 | 0.983 | 0.9415 | 0.9752 | 0.9181 | 0.9583 | 0.8228 | 0.9706 | 0.943 | 0.9215 | 0.9704 | 0.9504 | 0.8996 | 0.9684 | 0.9376 | 0.9785 | 0.936 | 0.9523 |
Cytoplasmic Foci | 0.0073 | 0.0007 | 0.0045 | 0.0015 | 0.0041 | 0.0051 | 0.0218 | 0.0035 | 0.006 | 0.0038 | 0.0016 | 0.0024 | 0.0113 | 0.0016 | 0.0048 | 0.0009 | 0.0121 | 0.0039 |
Eisosomes | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.007 | 0.0024 | 0.0074 | 0.0056 | 0.0064 | 0.0043 | 0.0065 | 0.0018 | 0.0017 | 0.0036 | 0.0055 | 0.002 | 0.0079 | 0.0021 | 0.0027 | 0.0014 | 0.003 | 0.0034 |
Endosome | 0.0051 | 0.0005 | 0.0083 | 0.0036 | 0.0349 | 0.007 | 0.0341 | 0.0005 | 0.002 | 0.0079 | 0.0007 | 0.0038 | 0.0087 | 0.0016 | 0.0107 | 0.0005 | 0.0024 | 0.0042 |
Golgi | 0.0008 | 0 | 0.0006 | 0.0002 | 0.0013 | 0.0008 | 0.0136 | 0.0002 | 0.0015 | 0.0062 | 0 | 0.001 | 0.0024 | 0.0002 | 0.002 | 0 | 0.0028 | 0.0003 |
Lipid Particles | 0.0012 | 0 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0136 | 0.0001 | 0.0012 | 0.0057 | 0 | 0.0009 | 0.0015 | 0.0005 | 0.003 | 0 | 0.001 | 0.0001 |
Mitochondria | 0.0004 | 0.0001 | 0.0018 | 0.0001 | 0.0021 | 0.0005 | 0.0028 | 0.0002 | 0.0008 | 0.0073 | 0.0001 | 0.0008 | 0.0033 | 0.0005 | 0.0055 | 0.0001 | 0.0003 | 0.0002 |
None | 0.0078 | 0.0011 | 0.008 | 0.0013 | 0.0026 | 0.0021 | 0.0043 | 0.0017 | 0.0011 | 0.0014 | 0.0009 | 0.0011 | 0.0058 | 0.003 | 0.003 | 0.0018 | 0.0028 | 0.0016 |
Nuclear Periphery | 0.0077 | 0.0021 | 0.0053 | 0.0038 | 0.0123 | 0.0038 | 0.0099 | 0.0017 | 0.0017 | 0.0034 | 0.0031 | 0.0023 | 0.0103 | 0.003 | 0.0027 | 0.0017 | 0.0027 | 0.0043 |
Nucleolus | 0.0005 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Nucleus | 0.0121 | 0.0079 | 0.0114 | 0.0063 | 0.0103 | 0.0128 | 0.014 | 0.0082 | 0.012 | 0.0276 | 0.0154 | 0.0226 | 0.0218 | 0.0147 | 0.0143 | 0.0139 | 0.0197 | 0.0258 |
Peroxisomes | 0.0051 | 0 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0116 | 0.0058 | 0.0072 | 0.0012 | 0 | 0.0007 | 0.0017 | 0.0002 | 0.0011 | 0 | 0.0128 | 0.0001 |
Punctate Nuclear | 0.0033 | 0.0004 | 0.0024 | 0.0003 | 0.0018 | 0.0014 | 0.0091 | 0.0012 | 0.002 | 0.0012 | 0.0005 | 0.0008 | 0.0029 | 0.0008 | 0.0019 | 0.0003 | 0.0008 | 0.0009 |
Vacuole | 0.0013 | 0.0005 | 0.0031 | 0.0011 | 0.0019 | 0.0011 | 0.0023 | 0.0003 | 0.0006 | 0.0007 | 0.0006 | 0.0008 | 0.0013 | 0.0005 | 0.0013 | 0.0003 | 0.0004 | 0.0006 |
Vacuole Periphery | 0.0003 | 0.0001 | 0.0005 | 0.0002 | 0.001 | 0.0004 | 0.0008 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 74.4722 | 56.6102 | 69.5639 | 67.5894 | 48.6067 | 88.102 | 69.9455 | 74.0192 | 90.6648 | 65.7731 |
Translational Efficiency | 0.8182 | 0.8522 | 0.7783 | 0.9844 | 0.7953 | 0.6943 | 0.8372 | 0.6939 | 0.6474 | 0.6621 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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