ORF
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.94 0.97 0.95 0.93 0.88 0.87 0.89 0.72 0.73 0.98 0.99 0.99 0.92 0.98 0.95 0.97 0.96 0.96 0.93 0.97 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0.08 0.07 0.05 0 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.19 0.18 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud 1 1 0 0 0 1 0 2 0 6 2 0 0 0 0 0 0 1 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 5 3 9 1 6 8 12 6 10 2 8 3 0 2 5 0 0 0 0 0 0 0 0
Cytoplasm 325 245 440 221 270 467 566 420 312 193 329 247 294 272 49 63 21 317 239 486 71 174 164
Endoplasmic Reticulum 1 3 7 0 12 18 52 34 17 7 27 3 2 1 2 3 0 1 0 1 0 1 4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 4 0 0 0
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Mitochondria 0 0 0 0 0 1 2 0 5 13 10 0 0 1 0 0 0 0 0 5 0 0 0
Nucleus 2 1 2 2 3 3 18 17 13 9 11 4 0 3 4 1 0 0 2 3 1 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 18 9 2 12 11 19 8 51 83 0 0 0 2 1 1 0 2 2 1 2 4
Unique Cell Count 328 250 466 229 284 500 644 481 350 268 450 252 296 276 53 64 22 326 248 509 77 181 180
Labelled Cell Count 335 254 476 233 293 511 661 498 366 282 471 257 296 279 62 68 22 326 248 509 77 181 180


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.4 18.4 12.4 11.7 12.7 11.6 12.2 12.2 10.8 9.2 11.2 15.9 16.9 16.6 25.2 23.6 21.0 13.6 14.0 16.3
Std Deviation (1e-4) 2.3 3.2 1.9 2.1 1.8 1.8 1.9 2.0 2.1 1.9 2.5 2.5 2.7 3.0 7.1 7.3 3.4 1.8 2.1 3.5
Intensity Change (Log2) -0.08 0.04 -0.1 -0.03 -0.02 -0.2 -0.44 -0.15 0.35 0.44 0.42 1.02 0.93 0.76 0.13 0.18 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.2 -0.4 -0.1 -0.8 0.9 0 -0.2 0 0 0 0 0 0
Cytoplasm 1.2 0.4 -0.7 -3.7 -3.8 -2.8 -8.5 -8.8 2.3 3.5 2.8 -0.6 1.4 0.2
Endoplasmic Reticulum 0 2.3 2.1 4.8 4.2 2.8 1.1 3.6 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 2.9 3.4 3.5 0 2.6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 -2.6 -1.3 -2.2 0.1 -1.3 6.8 7.0 -3.2 -3.4 -3.3 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ykr018c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available