Standard name | |
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Description | Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; links the (VFT/GARP) complex to the SNARE Tlg1p; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 5 | 4 | 0 | 4 | 2 | – | 2 | 0 | 2 | 2 | 2 | 6 | 1 | 0 | 0 | 2 | 1 | 31 | 21 | 4 | 2 | 3 | 5 |
Bud | 17 | 6 | 41 | 16 | 19 | 29 | – | 52 | 46 | 52 | 29 | 32 | 4 | 2 | 3 | 3 | 11 | 1 | 3 | 2 | 0 | 0 | 0 | 0 |
Bud Neck | 3 | 0 | 7 | 0 | 1 | 0 | – | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 8 | 0 | 4 | 1 | 7 | 5 | – | 16 | 11 | 16 | 12 | 12 | 0 | 0 | 0 | 1 | 1 | 2 | – | – | – | – | – | – |
Cell Periphery | 3 | 3 | 0 | 3 | 1 | 1 | – | 0 | 3 | 3 | 1 | 3 | 1 | 0 | 0 | 0 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 30 | 22 | 29 | 18 | 29 | 13 | – | 35 | 14 | 21 | 14 | 11 | 6 | 6 | 10 | 48 | 52 | 59 | 1 | 2 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 1 | 12 | 3 | 2 | 5 | 4 | – | 2 | 3 | 3 | 3 | 6 | 8 | 3 | 1 | 6 | 4 | 6 | 0 | 1 | 3 | 0 | 0 | 0 |
Endosome | 213 | 92 | 2 | 3 | 45 | 5 | – | 18 | 9 | 10 | 5 | 8 | 36 | 23 | 66 | 147 | 106 | 101 | 267 | 182 | 142 | 34 | 41 | 44 |
Golgi | 0 | 49 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 12 | 8 | 6 | 24 | 12 | 11 | 107 | 157 | 24 | 25 | 32 | 44 |
Mitochondria | 449 | 304 | 314 | 247 | 249 | 509 | – | 694 | 499 | 542 | 414 | 410 | 48 | 55 | 45 | 36 | 35 | 25 | 133 | 80 | 75 | 11 | 17 | 19 |
Nucleus | 2 | 0 | 0 | 2 | 2 | 2 | – | 6 | 2 | 7 | 2 | 1 | 0 | 2 | 1 | 8 | 8 | 7 | 2 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 3 | – | 5 | 5 | 2 | 10 | 7 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 2 | 0 | 0 | 0 |
Nucleolus | 13 | 3 | 0 | 4 | 6 | 16 | – | 28 | 17 | 8 | 15 | 10 | 0 | 0 | 1 | 4 | 8 | 7 | 3 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 166 | 67 | 13 | 41 | 80 | 23 | – | 65 | 17 | 61 | 30 | 33 | 19 | 7 | 31 | 80 | 100 | 89 | 155 | 97 | 125 | 43 | 40 | 37 |
SpindlePole | 81 | 22 | 50 | 19 | 40 | 33 | – | 94 | 32 | 51 | 36 | 24 | 9 | 4 | 19 | 161 | 117 | 120 | 23 | 12 | 15 | 1 | 2 | 4 |
Vac/Vac Membrane | 21 | 16 | 3 | 0 | 3 | 6 | – | 7 | 6 | 6 | 3 | 7 | 3 | 2 | 4 | 19 | 13 | 13 | 14 | 12 | 6 | 0 | 2 | 0 |
Unique Cell Count | 668 | 468 | 370 | 276 | 367 | 556 | 811 | 542 | 624 | 454 | 458 | 109 | 94 | 146 | 353 | 314 | 302 | 756 | 579 | 414 | 123 | 142 | 160 | |
Labelled Cell Count | 1008 | 601 | 470 | 356 | 492 | 651 | 1027 | 664 | 785 | 576 | 567 | 152 | 114 | 188 | 538 | 472 | 447 | 756 | 579 | 414 | 123 | 142 | 160 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 5.9 | 3.7 | 3.8 | 4.6 | 3.8 | – | 4.0 | 3.8 | 4.2 | 4.0 | 4.2 | 5.8 | 5.8 | 5.7 | 7.9 | 7.6 | 7.7 | 5.4 | 5.9 | 6.0 |
Std Deviation (1e-4) | 0.7 | 0.9 | 0.7 | 1.1 | 1.3 | 0.9 | – | 1.0 | 1.1 | 1.1 | 1.0 | 1.2 | 1.1 | 1.2 | 1.0 | 2.1 | 2.2 | 2.4 | 1.0 | 1.2 | 1.1 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.32 | 0.03 | – | 0.11 | 0.02 | 0.18 | 0.1 | 0.17 | 0.63 | 0.63 | 0.62 | 1.08 | 1.02 | 1.04 | 0.54 | 0.67 | 0.68 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -2.3 | -2.7 | -3.3 | -5.8 | -3.7 | -5.7 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | 0 | -3.9 | – | -2.5 | -3.7 | -3.1 | -3.1 | -3.6 | -0.8 | -0.5 | -0.4 | 2.5 | 3.5 | 4.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 11.0 | 9.2 | 13.5 | 13.7 | 11.9 | 11.8 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.1 | 3.8 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -8.7 | -5.7 | -12.0 | -20.1 | -19.2 | -19.8 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 5.1 | 0 | 6.5 | 7.7 | 9.9 | 9.3 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -1.5 | -2.5 | -0.2 | 9.5 | 7.2 | 7.8 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 2.9 | 3.0 |