Standard name
Description Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; links the (VFT/GARP) complex to the SNARE Tlg1p; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.11 0.06 0.05 0.05 – 0.06 0.08 0.08 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.08 0.07 0.08 0 – 0 0 0 0 0 0.06 0.06 0.07 0.14 0.17 0.2 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.32 0.2 0 0 0.12 0 – 0 0 0 0 0 0.33 0.24 0.45 0.42 0.34 0.33 0.35 0.31 0.34 0.28 0.29 0.28
Golgi 0 0.1 0 0 0 0 – 0 0 0 0 0 0.11 0.09 0 0.07 0 0 0.14 0.27 0.06 0.21 0.23 0.28
Mitochondria 0.67 0.65 0.85 0.89 0.68 0.92 – 0.86 0.92 0.87 0.91 0.9 0.44 0.59 0.31 0.1 0.11 0.08 0.18 0.14 0.18 0.09 0.12 0.12
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.25 0.14 0 0.15 0.22 0 – 0.08 0 0.1 0.07 0.07 0.17 0.07 0.21 0.23 0.32 0.29 0.21 0.17 0.3 0.35 0.28 0.23
SpindlePole 0.12 0.05 0.14 0.07 0.11 0.06 – 0.12 0.06 0.08 0.08 0.05 0.08 0 0.13 0.46 0.37 0.4 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 5 4 0 4 2 – 2 0 2 2 2 6 1 0 0 2 1 31 21 4 2 3 5
Bud 17 6 41 16 19 29 – 52 46 52 29 32 4 2 3 3 11 1 3 2 0 0 0 0
Bud Neck 3 0 7 0 1 0 – 3 0 1 0 0 0 0 0 1 0 0 3 1 0 0 0 0
Bud Site 8 0 4 1 7 5 – 16 11 16 12 12 0 0 0 1 1 2 – – – – – –
Cell Periphery 3 3 0 3 1 1 – 0 3 3 1 3 1 0 0 0 2 4 0 0 0 0 0 0
Cytoplasm 30 22 29 18 29 13 – 35 14 21 14 11 6 6 10 48 52 59 1 2 1 0 0 0
Endoplasmic Reticulum 1 12 3 2 5 4 – 2 3 3 3 6 8 3 1 6 4 6 0 1 3 0 0 0
Endosome 213 92 2 3 45 5 – 18 9 10 5 8 36 23 66 147 106 101 267 182 142 34 41 44
Golgi 0 49 0 0 0 0 – 0 0 0 0 1 12 8 6 24 12 11 107 157 24 25 32 44
Mitochondria 449 304 314 247 249 509 – 694 499 542 414 410 48 55 45 36 35 25 133 80 75 11 17 19
Nucleus 2 0 0 2 2 2 – 6 2 7 2 1 0 2 1 8 8 7 2 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 3 – 5 5 2 10 7 0 1 1 0 1 1 1 1 2 0 0 0
Nucleolus 13 3 0 4 6 16 – 28 17 8 15 10 0 0 1 4 8 7 3 0 0 0 0 0
Peroxisomes 166 67 13 41 80 23 – 65 17 61 30 33 19 7 31 80 100 89 155 97 125 43 40 37
SpindlePole 81 22 50 19 40 33 – 94 32 51 36 24 9 4 19 161 117 120 23 12 15 1 2 4
Vac/Vac Membrane 21 16 3 0 3 6 – 7 6 6 3 7 3 2 4 19 13 13 14 12 6 0 2 0
Unique Cell Count 668 468 370 276 367 556 811 542 624 454 458 109 94 146 353 314 302 756 579 414 123 142 160
Labelled Cell Count 1008 601 470 356 492 651 1027 664 785 576 567 152 114 188 538 472 447 756 579 414 123 142 160


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 5.9 3.7 3.8 4.6 3.8 – 4.0 3.8 4.2 4.0 4.2 5.8 5.8 5.7 7.9 7.6 7.7 5.4 5.9 6.0
Std Deviation (1e-4) 0.7 0.9 0.7 1.1 1.3 0.9 – 1.0 1.1 1.1 1.0 1.2 1.1 1.2 1.0 2.1 2.2 2.4 1.0 1.2 1.1
Intensity Change (Log2) – – – 0.03 0.32 0.03 – 0.11 0.02 0.18 0.1 0.17 0.63 0.63 0.62 1.08 1.02 1.04 0.54 0.67 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 -2.3 -2.7 -3.3 -5.8 -3.7 -5.7
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.6 0 -3.9 – -2.5 -3.7 -3.1 -3.1 -3.6 -0.8 -0.5 -0.4 2.5 3.5 4.5
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 11.0 9.2 13.5 13.7 11.9 11.8
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 5.1 3.8 0
Mitochondria 0 0 0 – 0 0 0 0 0 -8.7 -5.7 -12.0 -20.1 -19.2 -19.8
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 5.1 0 6.5 7.7 9.9 9.3
SpindlePole 0 0 0 – 0 0 0 0 0 -1.5 -2.5 -0.2 9.5 7.2 7.8
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 3.6 2.9 3.0