Standard name | |
---|---|
Human Ortholog | |
Description | Putative ATP-dependent RNA helicase of the DEAD-box family; involved in ribosomal biogenesis; required at post-transcriptional step for efficient retrotransposition; essential for growth under anaerobic conditions |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 2 | 6 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 5 |
Bud Neck | 0 | 0 | 0 | 2 | 1 | 1 | 5 | 1 | 0 | 0 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 0 | 6 | 8 | 12 | 9 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 1 | 1 | 5 | 10 | 9 | 6 | 12 | 4 | 5 | 4 | 4 | 0 | 5 | 6 | 4 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 7 | 3 | 1 | 4 | 5 | 10 | 8 | 20 | 12 | 7 | 3 | 9 | 0 | 3 | 0 | 1 | 2 | 1 | 1 | 3 | 3 | 1 | 10 | 9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 4 | 0 | 0 | 1 | 1 | 9 | 30 | 9 | 30 | 44 | 26 | 13 | 6 | 0 | 3 | 6 | 5 | 6 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleus | 29 | 23 | 76 | 59 | 34 | 29 | 38 | 56 | 43 | 52 | 17 | 22 | 13 | 28 | 13 | 22 | 29 | 27 | 16 | 26 | 60 | 14 | 13 | 8 |
Nuclear Periphery | 5 | 2 | 0 | 0 | 2 | 4 | 1 | 2 | 5 | 5 | 1 | 3 | 0 | 1 | 1 | 3 | 0 | 1 | 3 | 1 | 2 | 0 | 1 | 2 |
Nucleolus | 85 | 55 | 25 | 81 | 131 | 96 | 114 | 129 | 98 | 110 | 49 | 41 | 28 | 85 | 74 | 57 | 39 | 57 | 87 | 70 | 46 | 15 | 52 | 34 |
Peroxisomes | 0 | 1 | 0 | 1 | 1 | 2 | 5 | 1 | 3 | 0 | 0 | 3 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
SpindlePole | 2 | 1 | 2 | 24 | 72 | 47 | 56 | 72 | 42 | 45 | 21 | 13 | 0 | 15 | 8 | 2 | 2 | 2 | 6 | 3 | 2 | 1 | 8 | 5 |
Vac/Vac Membrane | 3 | 4 | 5 | 3 | 4 | 3 | 6 | 12 | 5 | 3 | 0 | 1 | 1 | 1 | 1 | 8 | 2 | 10 | 2 | 6 | 10 | 1 | 12 | 8 |
Unique Cell Count | 113 | 82 | 103 | 141 | 179 | 146 | 186 | 224 | 155 | 190 | 84 | 76 | 40 | 108 | 90 | 91 | 70 | 92 | 120 | 114 | 128 | 36 | 104 | 86 |
Labelled Cell Count | 136 | 90 | 110 | 181 | 262 | 211 | 275 | 324 | 256 | 286 | 127 | 112 | 48 | 141 | 108 | 105 | 85 | 106 | 120 | 114 | 128 | 36 | 104 | 86 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.3 | 9.7 | 8.1 | 7.8 | 8.1 | 6.4 | 6.3 | 6.5 | 5.7 | 5.5 | 5.3 | 6.2 | 8.4 | 9.7 | 8.6 | 15.5 | 15.2 | 16.0 | – | 8.8 | 8.8 |
Std Deviation (1e-4) | 2.0 | 2.2 | 1.7 | 1.9 | 2.3 | 2.0 | 2.7 | 1.5 | 1.5 | 1.5 | 2.0 | 2.0 | 2.0 | 2.6 | 2.8 | 6.6 | 6.0 | 5.7 | – | 2.3 | 2.7 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.0 | -0.33 | -0.35 | -0.31 | -0.51 | -0.56 | -0.61 | -0.38 | 0.06 | 0.26 | 0.09 | 0.94 | 0.92 | 0.99 | – | 0.12 | 0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 2.7 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 4.3 | 0 | 4.7 | 5.3 | 6.1 | 4.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -5.0 | -9.1 | -8.5 | -8.9 | -8.4 | -7.3 | -7.6 | -7.3 | -6.0 | -4.6 | -7.0 | -8.3 | -6.9 | -4.3 | -6.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 5.2 | 8.0 | 6.4 | 6.0 | 5.6 | 6.1 | 5.5 | 4.7 | 4.1 | 5.1 | 7.9 | 8.0 | 5.4 | 4.2 | 5.3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 3.8 | 7.0 | 5.9 | 5.7 | 6.1 | 5.3 | 4.8 | 4.8 | 3.6 | 0 | 3.2 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | 0 | 1.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.9443 | 6.292 | 4.5742 | 4.4406 | 5.9249 | 6.5528 | 7.0969 | 8.3339 | 7.5616 | 6.8796 | 4.918 | 8.4725 | 6.8965 | 8.0068 | 6.0789 | 4.8518 | 6.9396 | 6.0154 |
Actin | 0.0582 | 0 | 0.0075 | 0.0042 | 0.0004 | 0.0006 | 0.0233 | 0.0118 | 0.0001 | 0.001 | 0.0044 | 0.0038 | 0.0263 | 0 | 0.0094 | 0 | 0.0007 | 0.0002 |
Bud | 0.0014 | 0.0001 | 0.0006 | 0.0002 | 0.001 | 0.0001 | 0.0013 | 0.0005 | 0.0002 | 0.0003 | 0.0007 | 0.0006 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0002 |
Bud Neck | 0.0067 | 0.0006 | 0.0008 | 0.0012 | 0.0004 | 0.0005 | 0.0012 | 0.0007 | 0.0004 | 0.0006 | 0.001 | 0.0022 | 0.006 | 0.0003 | 0.0007 | 0.0004 | 0.0005 | 0.0013 |
Bud Periphery | 0.0035 | 0.0002 | 0.0014 | 0.0005 | 0.0022 | 0.0004 | 0.0018 | 0.0012 | 0.0004 | 0.0009 | 0.0016 | 0.0021 | 0.0008 | 0.0003 | 0.0002 | 0.0002 | 0.0008 | 0.001 |
Bud Site | 0.0058 | 0.0005 | 0.003 | 0.0006 | 0.0005 | 0.0002 | 0.0129 | 0.0015 | 0.0009 | 0.0012 | 0.004 | 0.0016 | 0.0136 | 0.0002 | 0.021 | 0.0002 | 0.0003 | 0.0002 |
Cell Periphery | 0.0011 | 0.0005 | 0.0021 | 0.0006 | 0.0002 | 0.0002 | 0.001 | 0.0008 | 0.0004 | 0.0004 | 0.0012 | 0.0016 | 0.0007 | 0.0007 | 0.0003 | 0.0001 | 0.0002 | 0.0008 |
Cytoplasm | 0.0528 | 0.0001 | 0.0077 | 0.0002 | 0.018 | 0.0018 | 0.0135 | 0.0089 | 0.0001 | 0 | 0.0001 | 0.0008 | 0.0029 | 0 | 0.0016 | 0 | 0.0002 | 0.0001 |
Cytoplasmic Foci | 0.0335 | 0.0018 | 0.0125 | 0.0036 | 0.001 | 0.0051 | 0.0196 | 0.0025 | 0.0005 | 0.0005 | 0.0032 | 0.0027 | 0.0246 | 0.0001 | 0.0312 | 0.0001 | 0.0021 | 0.0001 |
Eisosomes | 0.0008 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0093 | 0.0001 | 0.0108 | 0.0009 | 0.0027 | 0.0047 | 0.0047 | 0.004 | 0 | 0 | 0.0001 | 0.0019 | 0.0032 | 0 | 0.0002 | 0 | 0.0004 | 0.0001 |
Endosome | 0.047 | 0.0003 | 0.0132 | 0.008 | 0.014 | 0.0314 | 0.0438 | 0.0053 | 0.0006 | 0.0002 | 0.005 | 0.0243 | 0.0185 | 0.0002 | 0.0056 | 0.0001 | 0.0375 | 0.0002 |
Golgi | 0.0177 | 0.0001 | 0.0029 | 0.0085 | 0.0005 | 0.0049 | 0.012 | 0.0066 | 0.0002 | 0.0002 | 0.0046 | 0.0053 | 0.0089 | 0 | 0.0073 | 0 | 0.0181 | 0.0002 |
Lipid Particles | 0.0352 | 0.0015 | 0.0149 | 0.0523 | 0.0006 | 0.0034 | 0.0187 | 0.0023 | 0.0019 | 0.0026 | 0.0262 | 0.0086 | 0.0321 | 0.0007 | 0.0023 | 0.0008 | 0.0071 | 0.0033 |
Mitochondria | 0.0259 | 0.0005 | 0.026 | 0.0067 | 0.003 | 0.0029 | 0.0108 | 0.0076 | 0.0009 | 0.0014 | 0.1576 | 0.0096 | 0.01 | 0.0007 | 0.0131 | 0.0001 | 0.0785 | 0.0018 |
None | 0.0642 | 0.0001 | 0.0134 | 0.0004 | 0.0014 | 0.0011 | 0.0047 | 0.0006 | 0.0002 | 0.0003 | 0.0004 | 0.0007 | 0.001 | 0 | 0.0002 | 0 | 0.0004 | 0.0001 |
Nuclear Periphery | 0.0458 | 0.0221 | 0.0099 | 0.051 | 0.034 | 0.0281 | 0.0472 | 0.0219 | 0.0049 | 0.0075 | 0.0034 | 0.1176 | 0.0618 | 0.0042 | 0.007 | 0.0003 | 0.2494 | 0.0135 |
Nucleolus | 0.3483 | 0.7221 | 0.6808 | 0.6562 | 0.4437 | 0.7004 | 0.4569 | 0.608 | 0.7074 | 0.8745 | 0.6757 | 0.5255 | 0.6263 | 0.7202 | 0.7907 | 0.8883 | 0.3543 | 0.6639 |
Nucleus | 0.1687 | 0.2328 | 0.1454 | 0.1889 | 0.4376 | 0.1892 | 0.2427 | 0.2918 | 0.2625 | 0.1 | 0.087 | 0.2636 | 0.1377 | 0.2471 | 0.1006 | 0.107 | 0.221 | 0.3032 |
Peroxisomes | 0.0088 | 0.0001 | 0.0023 | 0.0021 | 0.0001 | 0.0002 | 0.0051 | 0.0005 | 0.0001 | 0.0003 | 0.0063 | 0.0015 | 0.0096 | 0 | 0.0027 | 0 | 0.0006 | 0.0001 |
Punctate Nuclear | 0.0527 | 0.0038 | 0.0066 | 0.0055 | 0.0065 | 0.0147 | 0.0546 | 0.001 | 0.0137 | 0.0059 | 0.0017 | 0.0047 | 0.0043 | 0.0165 | 0.0012 | 0.0014 | 0.0132 | 0.0021 |
Vacuole | 0.006 | 0.0065 | 0.0224 | 0.0034 | 0.0186 | 0.0028 | 0.0123 | 0.0106 | 0.002 | 0.0008 | 0.0079 | 0.0086 | 0.0048 | 0.0045 | 0.0006 | 0.0005 | 0.0036 | 0.0025 |
Vacuole Periphery | 0.0068 | 0.0062 | 0.0156 | 0.0052 | 0.0136 | 0.0075 | 0.0118 | 0.0118 | 0.0026 | 0.0012 | 0.0074 | 0.0124 | 0.0062 | 0.0041 | 0.0037 | 0.0003 | 0.0107 | 0.0052 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 26.2362 | 18.1433 | 13.1347 | 18.9679 | 18.9336 | 9.895 | 17.2261 | 12.0794 | 11.3497 | 22.4228 |
Translational Efficiency | 1.7038 | 1.9517 | 2.2183 | 0.9934 | 1.6487 | 2.3601 | 1.4242 | 1.1709 | 1.1133 | 1.1037 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1363 | 520 | 1603 | 271 | 1325 | 106 | 1699 | 127 | 2688 | 626 | 3302 | 398 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 845.41 | 1036.70 | 1020.24 | 1207.75 | 933.51 | 1117.73 | 1072.92 | 1328.62 | 888.84 | 1050.42 | 1047.35 | 1246.32 |
Standard Deviation | 152.51 | 176.26 | 164.58 | 260.31 | 154.53 | 185.21 | 220.17 | 254.12 | 159.70 | 180.38 | 196.94 | 264.42 |
Intensity Change Log 2 | — | 0.294275 | 0.271185 | 0.514599 | — | 0.259834 | 0.200805 | 0.509191 | — | 0.276305 | 0.234681 | 0.511764 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000422 | 0.000421 | 0.000510 | 0.000669 | 0.000402 | 0.000902 | 0.000570 | 0.000659 | 0.000412 | 0.000502 | 0.000541 | 0.000666 |
Bud Neck | 0.005853 | 0.006794 | 0.010324 | 0.014660 | 0.002947 | 0.009587 | 0.009151 | 0.017377 | 0.004421 | 0.007267 | 0.009721 | 0.015527 |
Bud Site | 0.002436 | 0.001183 | 0.000819 | 0.001425 | 0.000390 | 0.003844 | 0.001094 | 0.003151 | 0.001428 | 0.001633 | 0.000960 | 0.001976 |
Cell Periphery | 0.000153 | 0.000040 | 0.000039 | 0.000044 | 0.000043 | 0.000078 | 0.000070 | 0.000149 | 0.000099 | 0.000046 | 0.000055 | 0.000077 |
Cytoplasm | 0.011876 | 0.001316 | 0.000315 | 0.000182 | 0.000446 | 0.006932 | 0.002215 | 0.000507 | 0.006242 | 0.002267 | 0.001293 | 0.000286 |
Cytoplasmic Foci | 0.003411 | 0.001958 | 0.000192 | 0.003717 | 0.000296 | 0.009203 | 0.000730 | 0.001945 | 0.001876 | 0.003185 | 0.000469 | 0.003152 |
Eisosomes | 0.000057 | 0.000048 | 0.000044 | 0.000047 | 0.000049 | 0.000065 | 0.000046 | 0.000052 | 0.000053 | 0.000051 | 0.000045 | 0.000049 |
Endoplasmic Reticulum | 0.003064 | 0.002080 | 0.001589 | 0.001050 | 0.002340 | 0.004222 | 0.001833 | 0.001033 | 0.002707 | 0.002443 | 0.001715 | 0.001045 |
Endosome | 0.002652 | 0.002031 | 0.000619 | 0.007272 | 0.000655 | 0.004161 | 0.000912 | 0.001018 | 0.001667 | 0.002392 | 0.000770 | 0.005276 |
Golgi | 0.001456 | 0.000820 | 0.000311 | 0.003811 | 0.000460 | 0.003944 | 0.000340 | 0.000877 | 0.000965 | 0.001349 | 0.000326 | 0.002875 |
Lipid Particles | 0.000794 | 0.000339 | 0.000076 | 0.000911 | 0.000398 | 0.009247 | 0.000123 | 0.000230 | 0.000599 | 0.001848 | 0.000100 | 0.000694 |
Mitochondria | 0.001297 | 0.001285 | 0.001799 | 0.002673 | 0.001094 | 0.002813 | 0.001666 | 0.002031 | 0.001197 | 0.001544 | 0.001731 | 0.002468 |
Mitotic Spindle | 0.005113 | 0.015375 | 0.009371 | 0.003772 | 0.008035 | 0.038614 | 0.011735 | 0.047167 | 0.006554 | 0.019310 | 0.010587 | 0.017619 |
None | 0.011133 | 0.001960 | 0.000686 | 0.000953 | 0.001170 | 0.001499 | 0.003303 | 0.001955 | 0.006222 | 0.001882 | 0.002032 | 0.001273 |
Nuclear Periphery | 0.001608 | 0.002825 | 0.004477 | 0.003687 | 0.002743 | 0.005720 | 0.002855 | 0.012299 | 0.002167 | 0.003315 | 0.003643 | 0.006435 |
Nuclear Periphery Foci | 0.016071 | 0.016803 | 0.004872 | 0.001575 | 0.021245 | 0.023325 | 0.003376 | 0.015725 | 0.018621 | 0.017907 | 0.004102 | 0.006090 |
Nucleolus | 0.474361* | 0.504303* | 0.563269* | 0.535913* | 0.554111* | 0.446495* | 0.446482* | 0.636795* | 0.513672* | 0.494515* | 0.503178* | 0.568104* |
Nucleus | 0.451547* | 0.432658* | 0.395951* | 0.401212* | 0.399148* | 0.416347* | 0.508158* | 0.238864* | 0.425718* | 0.429896* | 0.453686* | 0.349408* |
Peroxisomes | 0.002106 | 0.003482 | 0.002725 | 0.007386 | 0.002786 | 0.006163 | 0.002516 | 0.008509 | 0.002441 | 0.003936 | 0.002617 | 0.007744 |
Vacuole | 0.003469 | 0.002886 | 0.000919 | 0.005821 | 0.000681 | 0.004065 | 0.001320 | 0.005685 | 0.002095 | 0.003086 | 0.001125 | 0.005777 |
Vacuole Periphery | 0.001119 | 0.001394 | 0.001094 | 0.003221 | 0.000562 | 0.002774 | 0.001503 | 0.003973 | 0.000845 | 0.001628 | 0.001305 | 0.003461 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.30 | -2.38 | -4.57 | -4.81 | -3.53 | -3.26 | -5.07 | -4.14 | 1.12 | -2.17 | -2.13 | -5.22 | -6.22 | -3.83 | -3.96 |
Bud Neck | -1.24 | -5.99 | -4.96 | -4.36 | -2.69 | -3.01 | -11.10 | -6.85 | -3.62 | -4.84 | -3.94 | -11.41 | -8.32 | -6.23 | -5.11 |
Bud Site | 2.55 | 3.49 | 1.01 | -1.53 | -2.55 | -2.02 | -5.83 | -5.28 | -0.24 | -4.38 | -0.79 | 1.60 | -3.35 | -2.03 | -4.70 |
Cell Periphery | 6.23 | 6.21 | 4.84 | -1.25 | -1.29 | -1.82 | -3.57 | -2.06 | -1.23 | -1.55 | 4.75 | 4.19 | 0.53 | -2.00 | -1.60 |
Cytoplasm | 6.26 | 6.91 | 6.85 | 2.00 | -0.55 | -1.70 | -3.68 | -0.75 | 1.66 | 3.13 | 3.71 | 5.58 | 6.68 | 2.34 | 3.00 |
Cytoplasmic Foci | 1.44 | 5.21 | 0.47 | -0.64 | -2.10 | -2.21 | -1.77 | -1.64 | 1.06 | -1.43 | -0.89 | 4.21 | -1.06 | -0.17 | -2.44 |
Eisosomes | 2.64 | 4.52 | 1.97 | -0.18 | -1.17 | -2.43 | 1.47 | -0.83 | 1.61 | -1.48 | 0.75 | 4.39 | 0.98 | 0.28 | -1.66 |
Endoplasmic Reticulum | 3.85 | 7.05 | 7.68 | 3.44 | 2.06 | -2.67 | 3.08 | 5.23 | 4.18 | 3.34 | 0.97 | 7.41 | 9.06 | 5.35 | 3.77 |
Endosome | 0.97 | 5.26 | -1.24 | -1.45 | -1.98 | -2.38 | -0.90 | -2.14 | 1.90 | -1.12 | -1.27 | 3.75 | -1.53 | -1.12 | -2.01 |
Golgi | 2.13 | 4.48 | -0.99 | -1.39 | -1.69 | -1.87 | 1.70 | -5.10 | 1.52 | -5.69 | -0.91 | 4.75 | -1.31 | -1.01 | -1.88 |
Lipid Particles | 2.72 | 5.27 | -0.00 | -0.82 | -1.27 | -1.42 | 3.81 | 0.79 | 1.43 | -2.49 | -1.15 | 6.40 | -0.12 | 1.04 | -1.40 |
Mitochondria | 0.13 | -3.34 | -2.55 | -2.47 | -1.46 | -2.16 | -4.19 | -4.26 | 0.25 | -2.39 | -1.50 | -5.28 | -3.77 | -2.57 | -2.17 |
Mitotic Spindle | -2.26 | -2.80 | -1.16 | 0.95 | 0.23 | -2.43 | -1.96 | -3.96 | -0.91 | -3.61 | -2.99 | -3.34 | -3.75 | -0.92 | -2.73 |
None | 6.52 | 8.00 | 7.66 | 1.50 | -1.62 | -0.79 | -4.20 | -0.72 | -0.29 | 1.22 | 5.34 | 5.83 | 6.46 | 0.93 | 1.62 |
Nuclear Periphery | -1.68 | -4.02 | -1.52 | -0.53 | 0.73 | -0.96 | -0.17 | -2.19 | -1.13 | -2.17 | -1.37 | -3.00 | -2.50 | -1.62 | -1.49 |
Nuclear Periphery Foci | -0.03 | 6.14 | 8.33 | 4.60 | 4.27 | -0.12 | 8.21 | 1.44 | 0.81 | -2.21 | 0.37 | 10.25 | 6.42 | 3.45 | -0.87 |
Nucleolus | -2.61 | -9.44 | -2.47 | -0.67 | 2.26 | 4.22 | 11.85 | -2.05 | -4.72 | -6.97 | 1.79 | 1.89 | -2.19 | -3.03 | -3.05 |
Nucleus | 1.44 | 5.88 | 2.35 | 1.26 | -0.60 | -0.65 | -11.81 | 8.99 | 5.57 | 14.96 | -0.45 | -4.53 | 5.03 | 4.59 | 7.16 |
Peroxisomes | -2.80 | -2.52 | -4.04 | -2.83 | -3.59 | -2.39 | 0.85 | -6.50 | -1.56 | -6.81 | -3.20 | -0.90 | -5.84 | -3.82 | -5.67 |
Vacuole | 0.82 | 3.71 | -1.44 | -1.66 | -2.17 | -3.00 | -2.82 | -2.92 | -0.91 | -2.56 | -1.32 | 2.48 | -2.35 | -1.95 | -2.72 |
Vacuole Periphery | -0.85 | 0.09 | -1.62 | -1.34 | -1.63 | -1.98 | -2.26 | -3.76 | -0.74 | -2.46 | -2.43 | -2.02 | -2.87 | -1.82 | -2.28 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|