Standard name
Human Ortholog
Description Putative ATP-dependent RNA helicase of the DEAD-box family; involved in ribosomal biogenesis; required at post-transcriptional step for efficient retrotransposition; essential for growth under anaerobic conditions

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.08 0.05 0.05 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0.06 0 0.05 0 0 0.05 0.05 0 0.05 0.07 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0.06 0 0 0 0 0.07 0 0.09 0.08 0 0 0.12 0 0 0 0 0 0 0 0 0 0 0.1 0.11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.16 0 0.19 0.23 0.31 0.17 0.15 0 0 0.07 0.07 0.07 0 0 0 0 0 0
Nucleus 0.26 0.28 0.74 0.42 0.19 0.2 0.2 0.25 0.28 0.27 0.2 0.29 0.33 0.26 0.14 0.24 0.41 0.29 0.14 0.24 0.47 0.4 0.13 0.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.75 0.67 0.24 0.57 0.73 0.66 0.61 0.58 0.63 0.58 0.58 0.54 0.7 0.79 0.82 0.63 0.56 0.62 0.73 0.62 0.37 0.44 0.5 0.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.17 0.4 0.32 0.3 0.32 0.27 0.24 0.25 0.17 0 0.14 0.09 0 0 0 0.06 0 0 0 0.08 0.07
Vac/Vac Membrane 0 0.05 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0 0.09 0 0.11 0 0.06 0.08 0.05 0.12 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 1 0 2 2 6 0 1 0 0 0 0 0 0 1 1 0 1 0 5
Bud Neck 0 0 0 2 1 1 5 1 0 0 2 1 0 1 1 0 0 0 0 0 0 0 1 3
Bud Site 0 0 0 0 1 0 6 8 12 9 4 1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 1 1 5 10 9 6 12 4 5 4 4 0 5 6 4 2 2 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 4 0 0 0 0 0 0 0
Endosome 7 3 1 4 5 10 8 20 12 7 3 9 0 3 0 1 2 1 1 3 3 1 10 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Mitochondria 4 0 0 1 1 9 30 9 30 44 26 13 6 0 3 6 5 6 0 0 0 0 0 2
Nucleus 29 23 76 59 34 29 38 56 43 52 17 22 13 28 13 22 29 27 16 26 60 14 13 8
Nuclear Periphery 5 2 0 0 2 4 1 2 5 5 1 3 0 1 1 3 0 1 3 1 2 0 1 2
Nucleolus 85 55 25 81 131 96 114 129 98 110 49 41 28 85 74 57 39 57 87 70 46 15 52 34
Peroxisomes 0 1 0 1 1 2 5 1 3 0 0 3 0 2 0 1 0 0 0 0 0 0 0 2
SpindlePole 2 1 2 24 72 47 56 72 42 45 21 13 0 15 8 2 2 2 6 3 2 1 8 5
Vac/Vac Membrane 3 4 5 3 4 3 6 12 5 3 0 1 1 1 1 8 2 10 2 6 10 1 12 8
Unique Cell Count 113 82 103 141 179 146 186 224 155 190 84 76 40 108 90 91 70 92 120 114 128 36 104 86
Labelled Cell Count 136 90 110 181 262 211 275 324 256 286 127 112 48 141 108 105 85 106 120 114 128 36 104 86


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.3 9.7 8.1 7.8 8.1 6.4 6.3 6.5 5.7 5.5 5.3 6.2 8.4 9.7 8.6 15.5 15.2 16.0 8.8 8.8
Std Deviation (1e-4) 2.0 2.2 1.7 1.9 2.3 2.0 2.7 1.5 1.5 1.5 2.0 2.0 2.0 2.6 2.8 6.6 6.0 5.7 2.3 2.7
Intensity Change (Log2) -0.05 0.0 -0.33 -0.35 -0.31 -0.51 -0.56 -0.61 -0.38 0.06 0.26 0.09 0.94 0.92 0.99 0.12 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 2.7 2.4 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.3 0 4.7 5.3 6.1 4.4 0 0 0 0 0 0
Nucleus -5.0 -9.1 -8.5 -8.9 -8.4 -7.3 -7.6 -7.3 -6.0 -4.6 -7.0 -8.3 -6.9 -4.3 -6.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 5.2 8.0 6.4 6.0 5.6 6.1 5.5 4.7 4.1 5.1 7.9 8.0 5.4 4.2 5.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 3.8 7.0 5.9 5.7 6.1 5.3 4.8 4.8 3.6 0 3.2 0 0 0 0
Vacuole 0 0 0 0 0.2 0 0 0 0 0 0 0 1.1 0 1.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9443 6.292 4.5742 4.4406 5.9249 6.5528 7.0969 8.3339 7.5616 6.8796 4.918 8.4725 6.8965 8.0068 6.0789 4.8518 6.9396 6.0154
Actin 0.0582 0 0.0075 0.0042 0.0004 0.0006 0.0233 0.0118 0.0001 0.001 0.0044 0.0038 0.0263 0 0.0094 0 0.0007 0.0002
Bud 0.0014 0.0001 0.0006 0.0002 0.001 0.0001 0.0013 0.0005 0.0002 0.0003 0.0007 0.0006 0.0006 0.0001 0.0002 0.0001 0.0003 0.0002
Bud Neck 0.0067 0.0006 0.0008 0.0012 0.0004 0.0005 0.0012 0.0007 0.0004 0.0006 0.001 0.0022 0.006 0.0003 0.0007 0.0004 0.0005 0.0013
Bud Periphery 0.0035 0.0002 0.0014 0.0005 0.0022 0.0004 0.0018 0.0012 0.0004 0.0009 0.0016 0.0021 0.0008 0.0003 0.0002 0.0002 0.0008 0.001
Bud Site 0.0058 0.0005 0.003 0.0006 0.0005 0.0002 0.0129 0.0015 0.0009 0.0012 0.004 0.0016 0.0136 0.0002 0.021 0.0002 0.0003 0.0002
Cell Periphery 0.0011 0.0005 0.0021 0.0006 0.0002 0.0002 0.001 0.0008 0.0004 0.0004 0.0012 0.0016 0.0007 0.0007 0.0003 0.0001 0.0002 0.0008
Cytoplasm 0.0528 0.0001 0.0077 0.0002 0.018 0.0018 0.0135 0.0089 0.0001 0 0.0001 0.0008 0.0029 0 0.0016 0 0.0002 0.0001
Cytoplasmic Foci 0.0335 0.0018 0.0125 0.0036 0.001 0.0051 0.0196 0.0025 0.0005 0.0005 0.0032 0.0027 0.0246 0.0001 0.0312 0.0001 0.0021 0.0001
Eisosomes 0.0008 0 0.0002 0.0001 0 0 0.0003 0.0001 0 0.0001 0.0005 0.0001 0.0002 0 0.0001 0 0.0001 0.0001
Endoplasmic Reticulum 0.0093 0.0001 0.0108 0.0009 0.0027 0.0047 0.0047 0.004 0 0 0.0001 0.0019 0.0032 0 0.0002 0 0.0004 0.0001
Endosome 0.047 0.0003 0.0132 0.008 0.014 0.0314 0.0438 0.0053 0.0006 0.0002 0.005 0.0243 0.0185 0.0002 0.0056 0.0001 0.0375 0.0002
Golgi 0.0177 0.0001 0.0029 0.0085 0.0005 0.0049 0.012 0.0066 0.0002 0.0002 0.0046 0.0053 0.0089 0 0.0073 0 0.0181 0.0002
Lipid Particles 0.0352 0.0015 0.0149 0.0523 0.0006 0.0034 0.0187 0.0023 0.0019 0.0026 0.0262 0.0086 0.0321 0.0007 0.0023 0.0008 0.0071 0.0033
Mitochondria 0.0259 0.0005 0.026 0.0067 0.003 0.0029 0.0108 0.0076 0.0009 0.0014 0.1576 0.0096 0.01 0.0007 0.0131 0.0001 0.0785 0.0018
None 0.0642 0.0001 0.0134 0.0004 0.0014 0.0011 0.0047 0.0006 0.0002 0.0003 0.0004 0.0007 0.001 0 0.0002 0 0.0004 0.0001
Nuclear Periphery 0.0458 0.0221 0.0099 0.051 0.034 0.0281 0.0472 0.0219 0.0049 0.0075 0.0034 0.1176 0.0618 0.0042 0.007 0.0003 0.2494 0.0135
Nucleolus 0.3483 0.7221 0.6808 0.6562 0.4437 0.7004 0.4569 0.608 0.7074 0.8745 0.6757 0.5255 0.6263 0.7202 0.7907 0.8883 0.3543 0.6639
Nucleus 0.1687 0.2328 0.1454 0.1889 0.4376 0.1892 0.2427 0.2918 0.2625 0.1 0.087 0.2636 0.1377 0.2471 0.1006 0.107 0.221 0.3032
Peroxisomes 0.0088 0.0001 0.0023 0.0021 0.0001 0.0002 0.0051 0.0005 0.0001 0.0003 0.0063 0.0015 0.0096 0 0.0027 0 0.0006 0.0001
Punctate Nuclear 0.0527 0.0038 0.0066 0.0055 0.0065 0.0147 0.0546 0.001 0.0137 0.0059 0.0017 0.0047 0.0043 0.0165 0.0012 0.0014 0.0132 0.0021
Vacuole 0.006 0.0065 0.0224 0.0034 0.0186 0.0028 0.0123 0.0106 0.002 0.0008 0.0079 0.0086 0.0048 0.0045 0.0006 0.0005 0.0036 0.0025
Vacuole Periphery 0.0068 0.0062 0.0156 0.0052 0.0136 0.0075 0.0118 0.0118 0.0026 0.0012 0.0074 0.0124 0.0062 0.0041 0.0037 0.0003 0.0107 0.0052

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.2362 18.1433 13.1347 18.9679 18.9336 9.895 17.2261 12.0794 11.3497 22.4228
Translational Efficiency 1.7038 1.9517 2.2183 0.9934 1.6487 2.3601 1.4242 1.1709 1.1133 1.1037

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1363 520 1603 271 1325 106 1699 127 2688 626 3302 398

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 845.41 1036.70 1020.24 1207.75 933.51 1117.73 1072.92 1328.62 888.84 1050.42 1047.35 1246.32
Standard Deviation 152.51 176.26 164.58 260.31 154.53 185.21 220.17 254.12 159.70 180.38 196.94 264.42
Intensity Change Log 2 0.294275 0.271185 0.514599 0.259834 0.200805 0.509191 0.276305 0.234681 0.511764

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000422 0.000421 0.000510 0.000669 0.000402 0.000902 0.000570 0.000659 0.000412 0.000502 0.000541 0.000666
Bud Neck 0.005853 0.006794 0.010324 0.014660 0.002947 0.009587 0.009151 0.017377 0.004421 0.007267 0.009721 0.015527
Bud Site 0.002436 0.001183 0.000819 0.001425 0.000390 0.003844 0.001094 0.003151 0.001428 0.001633 0.000960 0.001976
Cell Periphery 0.000153 0.000040 0.000039 0.000044 0.000043 0.000078 0.000070 0.000149 0.000099 0.000046 0.000055 0.000077
Cytoplasm 0.011876 0.001316 0.000315 0.000182 0.000446 0.006932 0.002215 0.000507 0.006242 0.002267 0.001293 0.000286
Cytoplasmic Foci 0.003411 0.001958 0.000192 0.003717 0.000296 0.009203 0.000730 0.001945 0.001876 0.003185 0.000469 0.003152
Eisosomes 0.000057 0.000048 0.000044 0.000047 0.000049 0.000065 0.000046 0.000052 0.000053 0.000051 0.000045 0.000049
Endoplasmic Reticulum 0.003064 0.002080 0.001589 0.001050 0.002340 0.004222 0.001833 0.001033 0.002707 0.002443 0.001715 0.001045
Endosome 0.002652 0.002031 0.000619 0.007272 0.000655 0.004161 0.000912 0.001018 0.001667 0.002392 0.000770 0.005276
Golgi 0.001456 0.000820 0.000311 0.003811 0.000460 0.003944 0.000340 0.000877 0.000965 0.001349 0.000326 0.002875
Lipid Particles 0.000794 0.000339 0.000076 0.000911 0.000398 0.009247 0.000123 0.000230 0.000599 0.001848 0.000100 0.000694
Mitochondria 0.001297 0.001285 0.001799 0.002673 0.001094 0.002813 0.001666 0.002031 0.001197 0.001544 0.001731 0.002468
Mitotic Spindle 0.005113 0.015375 0.009371 0.003772 0.008035 0.038614 0.011735 0.047167 0.006554 0.019310 0.010587 0.017619
None 0.011133 0.001960 0.000686 0.000953 0.001170 0.001499 0.003303 0.001955 0.006222 0.001882 0.002032 0.001273
Nuclear Periphery 0.001608 0.002825 0.004477 0.003687 0.002743 0.005720 0.002855 0.012299 0.002167 0.003315 0.003643 0.006435
Nuclear Periphery Foci 0.016071 0.016803 0.004872 0.001575 0.021245 0.023325 0.003376 0.015725 0.018621 0.017907 0.004102 0.006090
Nucleolus 0.474361* 0.504303* 0.563269* 0.535913* 0.554111* 0.446495* 0.446482* 0.636795* 0.513672* 0.494515* 0.503178* 0.568104*
Nucleus 0.451547* 0.432658* 0.395951* 0.401212* 0.399148* 0.416347* 0.508158* 0.238864* 0.425718* 0.429896* 0.453686* 0.349408*
Peroxisomes 0.002106 0.003482 0.002725 0.007386 0.002786 0.006163 0.002516 0.008509 0.002441 0.003936 0.002617 0.007744
Vacuole 0.003469 0.002886 0.000919 0.005821 0.000681 0.004065 0.001320 0.005685 0.002095 0.003086 0.001125 0.005777
Vacuole Periphery 0.001119 0.001394 0.001094 0.003221 0.000562 0.002774 0.001503 0.003973 0.000845 0.001628 0.001305 0.003461

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.30 -2.38 -4.57 -4.81 -3.53 -3.26 -5.07 -4.14 1.12 -2.17 -2.13 -5.22 -6.22 -3.83 -3.96
Bud Neck -1.24 -5.99 -4.96 -4.36 -2.69 -3.01 -11.10 -6.85 -3.62 -4.84 -3.94 -11.41 -8.32 -6.23 -5.11
Bud Site 2.55 3.49 1.01 -1.53 -2.55 -2.02 -5.83 -5.28 -0.24 -4.38 -0.79 1.60 -3.35 -2.03 -4.70
Cell Periphery 6.23 6.21 4.84 -1.25 -1.29 -1.82 -3.57 -2.06 -1.23 -1.55 4.75 4.19 0.53 -2.00 -1.60
Cytoplasm 6.26 6.91 6.85 2.00 -0.55 -1.70 -3.68 -0.75 1.66 3.13 3.71 5.58 6.68 2.34 3.00
Cytoplasmic Foci 1.44 5.21 0.47 -0.64 -2.10 -2.21 -1.77 -1.64 1.06 -1.43 -0.89 4.21 -1.06 -0.17 -2.44
Eisosomes 2.64 4.52 1.97 -0.18 -1.17 -2.43 1.47 -0.83 1.61 -1.48 0.75 4.39 0.98 0.28 -1.66
Endoplasmic Reticulum 3.85 7.05 7.68 3.44 2.06 -2.67 3.08 5.23 4.18 3.34 0.97 7.41 9.06 5.35 3.77
Endosome 0.97 5.26 -1.24 -1.45 -1.98 -2.38 -0.90 -2.14 1.90 -1.12 -1.27 3.75 -1.53 -1.12 -2.01
Golgi 2.13 4.48 -0.99 -1.39 -1.69 -1.87 1.70 -5.10 1.52 -5.69 -0.91 4.75 -1.31 -1.01 -1.88
Lipid Particles 2.72 5.27 -0.00 -0.82 -1.27 -1.42 3.81 0.79 1.43 -2.49 -1.15 6.40 -0.12 1.04 -1.40
Mitochondria 0.13 -3.34 -2.55 -2.47 -1.46 -2.16 -4.19 -4.26 0.25 -2.39 -1.50 -5.28 -3.77 -2.57 -2.17
Mitotic Spindle -2.26 -2.80 -1.16 0.95 0.23 -2.43 -1.96 -3.96 -0.91 -3.61 -2.99 -3.34 -3.75 -0.92 -2.73
None 6.52 8.00 7.66 1.50 -1.62 -0.79 -4.20 -0.72 -0.29 1.22 5.34 5.83 6.46 0.93 1.62
Nuclear Periphery -1.68 -4.02 -1.52 -0.53 0.73 -0.96 -0.17 -2.19 -1.13 -2.17 -1.37 -3.00 -2.50 -1.62 -1.49
Nuclear Periphery Foci -0.03 6.14 8.33 4.60 4.27 -0.12 8.21 1.44 0.81 -2.21 0.37 10.25 6.42 3.45 -0.87
Nucleolus -2.61 -9.44 -2.47 -0.67 2.26 4.22 11.85 -2.05 -4.72 -6.97 1.79 1.89 -2.19 -3.03 -3.05
Nucleus 1.44 5.88 2.35 1.26 -0.60 -0.65 -11.81 8.99 5.57 14.96 -0.45 -4.53 5.03 4.59 7.16
Peroxisomes -2.80 -2.52 -4.04 -2.83 -3.59 -2.39 0.85 -6.50 -1.56 -6.81 -3.20 -0.90 -5.84 -3.82 -5.67
Vacuole 0.82 3.71 -1.44 -1.66 -2.17 -3.00 -2.82 -2.92 -0.91 -2.56 -1.32 2.48 -2.35 -1.95 -2.72
Vacuole Periphery -0.85 0.09 -1.62 -1.34 -1.63 -1.98 -2.26 -3.76 -0.74 -2.46 -2.43 -2.02 -2.87 -1.82 -2.28