Standard name
Description Putative ATP-dependent RNA helicase of the DEAD-box family; involved in ribosomal biogenesis; required at post-transcriptional step for efficient retrotransposition; essential for growth under anaerobic conditions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.08 0.05 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0.06 0 0.05 0 0 0.05 0.05 0 0.05 0.07 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0.06 0 0 0 0 0.07 0 0.09 0.08 0 0 0.12 0 0 0 0 0 0 0 0 0 0 0.1 0.11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.16 0 0.19 0.23 0.31 0.17 0.15 0 0 0.07 0.07 0.07 0 0 0 0 0 0
Nucleus 0.26 0.28 0.74 0.42 0.19 0.2 0.2 0.25 0.28 0.27 0.2 0.29 0.33 0.26 0.14 0.24 0.41 0.29 0.14 0.24 0.47 0.4 0.13 0.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.75 0.67 0.24 0.57 0.73 0.66 0.61 0.58 0.63 0.58 0.58 0.54 0.7 0.79 0.82 0.63 0.56 0.62 0.73 0.62 0.37 0.44 0.5 0.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.17 0.4 0.32 0.3 0.32 0.27 0.24 0.25 0.17 0 0.14 0.09 0 0 0 0.06 0 0 0 0.08 0.07
Vac/Vac Membrane 0 0.05 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0 0.09 0 0.11 0 0.06 0.08 0.05 0.12 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 1 0 2 2 6 0 1 0 0 0 0 0 0 1 1 0 1 0 5
Bud Neck 0 0 0 2 1 1 5 1 0 0 2 1 0 1 1 0 0 0 0 0 0 0 1 3
Bud Site 0 0 0 0 1 0 6 8 12 9 4 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 1 1 5 10 9 6 12 4 5 4 4 0 5 6 4 2 2 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 4 0 0 0 0 0 0 0
Endosome 7 3 1 4 5 10 8 20 12 7 3 9 0 3 0 1 2 1 1 3 3 1 10 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Mitochondria 4 0 0 1 1 9 30 9 30 44 26 13 6 0 3 6 5 6 0 0 0 0 0 2
Nucleus 29 23 76 59 34 29 38 56 43 52 17 22 13 28 13 22 29 27 16 26 60 14 13 8
Nuclear Periphery 5 2 0 0 2 4 1 2 5 5 1 3 0 1 1 3 0 1 3 1 2 0 1 2
Nucleolus 85 55 25 81 131 96 114 129 98 110 49 41 28 85 74 57 39 57 87 70 46 15 52 34
Peroxisomes 0 1 0 1 1 2 5 1 3 0 0 3 0 2 0 1 0 0 0 0 0 0 0 2
SpindlePole 2 1 2 24 72 47 56 72 42 45 21 13 0 15 8 2 2 2 6 3 2 1 8 5
Vac/Vac Membrane 3 4 5 3 4 3 6 12 5 3 0 1 1 1 1 8 2 10 2 6 10 1 12 8
Unique Cell Count 113 82 103 141 179 146 186 224 155 190 84 76 40 108 90 91 70 92 120 114 128 36 104 86
Labelled Cell Count 136 90 110 181 262 211 275 324 256 286 127 112 48 141 108 105 85 106 120 114 128 36 104 86


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.3 9.7 8.1 7.8 8.1 6.4 6.3 6.5 5.7 5.5 5.3 6.2 8.4 9.7 8.6 15.5 15.2 16.0 – 8.8 8.8
Std Deviation (1e-4) 2.0 2.2 1.7 1.9 2.3 2.0 2.7 1.5 1.5 1.5 2.0 2.0 2.0 2.6 2.8 6.6 6.0 5.7 – 2.3 2.7
Intensity Change (Log2) – – – -0.05 0.0 -0.33 -0.35 -0.31 -0.51 -0.56 -0.61 -0.38 0.06 0.26 0.09 0.94 0.92 0.99 – 0.12 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 2.7 2.4 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.3 0 4.7 5.3 6.1 4.4 0 0 0 0 0 0
Nucleus -5.0 -9.1 -8.5 -8.9 -8.4 -7.3 -7.6 -7.3 -6.0 -4.6 -7.0 -8.3 -6.9 -4.3 -6.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 5.2 8.0 6.4 6.0 5.6 6.1 5.5 4.7 4.1 5.1 7.9 8.0 5.4 4.2 5.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 3.8 7.0 5.9 5.7 6.1 5.3 4.8 4.8 3.6 0 3.2 0 0 0 0
Vacuole 0 0 0 0 0.2 0 0 0 0 0 0 0 1.1 0 1.6