Standard name
Human Ortholog
Description General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.34 0.24 0.27 0.38 0.56 0.6 0.83 0.73 0.85 0.07 0.16 0.14 0 0.1 0.05 0 0 0
Endoplasmic Reticulum 0.19 0.07 0.07 0.06 0 0 0 0 0.07 0.07 0 0.05 0 0.09 0.13 0 0.89 0.86
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0
Golgi 0 0.05 0.05 0 0 0 0 0 0 0.05 0.12 0.14 0 0.21 0.41 0 0 0
Mitochondria 0 0 0.12 0 0 0 0 0 0 0.07 0.07 0.09 0 0 0 0 0 0
Nucleus 0 0.05 0 0 0 0 0 0 0 0.07 0.14 0.05 0.11 0.07 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0 0 0 0 0
Nucleolus 0.44 0.46 0.56 0.34 0.25 0.28 0.1 0.2 0.08 0.24 0.12 0.18 0.08 0 0 0.07 0 0.14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.24 0.07 0.26 0.24 0.1 0 0.05 0 0.61 0.47 0.59 0.5 0.31 0.19 0.92 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 0 0 9 6 0 0 0
Bud 0 0 0 0 1 0 1 0 0 1 0 0 0 2 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 31 20 11 20 50 52 93 62 62 7 7 3 0 20 8 0 0 0
Endoplasmic Reticulum 17 6 3 3 1 2 3 1 5 7 1 1 0 19 25 0 15 5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 13 9 0 0 0
Golgi 0 4 2 2 1 1 0 1 0 5 5 3 0 43 79 0 0 0
Mitochondria 4 3 5 1 2 0 0 2 1 7 3 2 0 0 1 0 0 0
Nucleus 1 4 0 1 3 1 2 3 0 7 6 1 0 14 5 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 40 39 23 18 22 24 11 17 6 24 5 4 0 4 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0
Vac/Vac Membrane 5 20 3 14 21 9 5 4 1 61 20 13 2 66 36 0 1 0
Unique Cell Count 90 84 41 53 89 86 112 85 73 100 43 22 5 212 193 1 17 7
Labelled Cell Count 98 96 48 59 101 89 115 91 75 119 49 27 5 212 193 1 17 7


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 219.2 200.9 202.3 250.1 302.2 268.6 343.5 312.2 350.8 120.0 94.0 174.7
Std Deviation (1e-4) 56.4 58.7 67.9 89.7 98.2 105.5 97.2 99.7 95.2 52.2 57.7 64.5
Intensity Change (Log2) 0.01 0.32 0.59 0.42 0.77 0.64 0.8 -0.74 -1.1 -0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 1.7 4.3 4.8 8.3 6.4 7.6 -3.2 -1.0 0
Endoplasmic Reticulum 0 0 0 0 0 0 -0.1 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.6 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.0 -1.4 -3.0 -2.5 -5.8 -3.6 -5.3 -3.2 -3.9 -2.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0
Vacuole -2.2 0.3 0 -2.3 -4.0 -3.6 -4.1 5.1 2.6 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.8859 18.7686 19.0486 12.9947 10.2781 18.9405 14.7031 17.7536 13.563 9.5368 10.1705 17.5281 15.0466 18.4523 17.0799 13.5228 9.2288 16.3433
Actin 0.0078 0.0001 0.0027 0.0166 0.0696 0.0001 0.0112 0.0005 0.0016 0.0174 0.0843 0.0001 0.0002 0 0.0275 0 0 0.0001
Bud 0.0009 0.0004 0.001 0.0017 0.0044 0.0014 0.0006 0.0007 0.0004 0.0013 0.0008 0.0006 0 0.0003 0.0005 0.0002 0.0001 0.0003
Bud Neck 0.0017 0.0008 0.0041 0.0008 0.003 0.0021 0.0013 0.0019 0.0013 0.002 0.0018 0.0011 0.0001 0.0015 0.0009 0.0006 0.0003 0.0018
Bud Periphery 0.0015 0.0008 0.0017 0.003 0.0051 0.0027 0.0018 0.0021 0.0013 0.0046 0.0015 0.0022 0.0001 0.0009 0.0012 0.0004 0.0003 0.0013
Bud Site 0.0033 0.0011 0.0185 0.0024 0.0043 0.0013 0.0151 0.0023 0.0015 0.0019 0.0063 0.0008 0 0.0011 0.0011 0.0004 0.0001 0.0007
Cell Periphery 0.008 0.0087 0.0055 0.0039 0.0033 0.0068 0.0085 0.0179 0.0041 0.0071 0.0115 0.0082 0.0005 0.0191 0.0043 0.0026 0.0037 0.0043
Cytoplasm 0.041 0.0161 0.004 0.0249 0.01 0.0019 0.0152 0.0083 0.0023 0.0036 0.0015 0.0039 0.0255 0.0009 0.092 0.0011 0.0002 0.0625
Cytoplasmic Foci 0.0521 0.005 0.0103 0.033 0.089 0.009 0.0103 0.0082 0.0127 0.0202 0.0141 0.0022 0.0081 0.0028 0.0168 0.0031 0.002 0.0055
Eisosomes 0.0001 0 0.0001 0.0002 0.0004 0 0.0002 0 0.0001 0.0002 0.0007 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0103 0.0047 0.0051 0.0126 0.0028 0.0029 0.0721 0.0283 0.0107 0.0032 0.0039 0.0543 0.005 0.0011 0.0219 0.0005 0.0005 0.0202
Endosome 0.1202 0.0803 0.0952 0.1134 0.0739 0.0572 0.1067 0.0514 0.1302 0.1221 0.118 0.0886 0.3001 0.0732 0.0659 0.1122 0.0588 0.0763
Golgi 0.0204 0.0089 0.0104 0.0048 0.0147 0.0056 0.016 0.0066 0.0215 0.0103 0.0543 0.012 0.0065 0.0034 0.0301 0.0045 0.0035 0.0086
Lipid Particles 0.045 0.0101 0.0121 0.0213 0.0291 0.0153 0.0271 0.0333 0.024 0.0341 0.0299 0.0223 0.0022 0.0397 0.0223 0.0039 0.0918 0.0496
Mitochondria 0.0211 0.0049 0.0292 0.011 0.0066 0.0021 0.0198 0.0031 0.0302 0.0113 0.0118 0.0111 0.0007 0.0353 0.1157 0.0007 0.0005 0.0104
None 0.0081 0.0001 0.0002 0.0051 0.0307 0.0001 0.0052 0.0002 0.0002 0.0025 0.0006 0.0001 0.2923 0.0001 0.0003 0 0 0.0003
Nuclear Periphery 0.0421 0.0161 0.0155 0.01 0.0041 0.0048 0.0555 0.011 0.0119 0.0041 0.0037 0.046 0.0055 0.0035 0.0089 0.001 0.009 0.0156
Nucleolus 0.0075 0.0055 0.0158 0.0157 0.0024 0.009 0.0027 0.0088 0.0019 0.0044 0.0097 0.0045 0.0011 0.0097 0.0022 0.0022 0.0024 0.0201
Nucleus 0.0354 0.0072 0.0126 0.0073 0.0025 0.0059 0.0081 0.006 0.0024 0.0049 0.0033 0.0046 0.0053 0.0038 0.017 0.0016 0.0019 0.0391
Peroxisomes 0.0042 0.0002 0.0032 0.0008 0.0137 0.0008 0.0084 0.0002 0.0082 0.0037 0.0054 0.0002 0.0001 0.0004 0.0568 0.0002 0.0001 0.0009
Punctate Nuclear 0.0544 0.0001 0.0003 0.0021 0.0117 0.0005 0.0011 0.0001 0.0003 0.0011 0.0005 0.0001 0.0061 0.0001 0.0005 0 0.0001 0.0022
Vacuole 0.333 0.5948 0.495 0.5188 0.4619 0.6419 0.4653 0.6142 0.4931 0.565 0.5074 0.5635 0.1554 0.4673 0.284 0.6199 0.5668 0.4065
Vacuole Periphery 0.1819 0.2343 0.2577 0.1905 0.157 0.2286 0.1478 0.1949 0.2402 0.1749 0.1292 0.1735 0.1847 0.3357 0.23 0.2447 0.2578 0.2739

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.6758 41.4398 31.9639 33.6739 25.702 17.0692 14.4719 15.8142 20.88 18.257
Translational Efficiency 1.3635 1.0457 0.9591 1.123 1.2481 0.6845 1.0682 0.7465 0.8667 0.836

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency