Standard name
Human Ortholog
Description General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 – 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 – 0 0 0 – 0 0 0 – – – 0 0 0 0.09 0 0 0 0 0
Bud Neck – 0 0 0 – 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 – 0 0 0 – 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery – 0 0 0 – 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.34 0.24 0.27 – 0.38 0.56 0.6 – 0.83 0.73 0.85 – – – 0.07 0.16 0.14 0 0.1 0.05 0 0 0
Endoplasmic Reticulum – 0.19 0.07 0.07 – 0.06 0 0 – 0 0 0.07 – – – 0.07 0 0.05 0 0.09 0.13 0 0.89 0.86
Endosome – 0 0 0 – 0 0 0 – 0 0 0 – – – 0 0 0 0 0.06 0.05 0 0 0
Golgi – 0 0.05 0.05 – 0 0 0 – 0 0 0 – – – 0.05 0.12 0.14 0 0.21 0.41 0 0 0
Mitochondria – 0 0 0.12 – 0 0 0 – 0 0 0 – – – 0.07 0.07 0.09 0 0 0 0 0 0
Nucleus – 0 0.05 0 – 0 0 0 – 0 0 0 – – – 0.07 0.14 0.05 0.11 0.07 0 0 0 0
Nuclear Periphery – 0 0 0 – 0 0 0 – 0 0 0 – – – 0 0 0 0.2 0 0 0 0 0
Nucleolus – 0.44 0.46 0.56 – 0.34 0.25 0.28 – 0.1 0.2 0.08 – – – 0.24 0.12 0.18 0.08 0 0 0.07 0 0.14
Peroxisomes – 0 0 0 – 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 – 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0.06 0.24 0.07 – 0.26 0.24 0.1 – 0 0.05 0 – – – 0.61 0.47 0.59 0.5 0.31 0.19 0.92 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 – 0 0 0 – 0 0 0 – – – 0 1 0 0 9 6 0 0 0
Bud – 0 0 0 – 0 1 0 – 1 0 0 – – – 1 0 0 0 2 1 0 0 0
Bud Neck – 0 0 0 – 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 – 0 0 0 – 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery – 0 0 1 – 0 0 0 – 0 1 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm – 31 20 11 – 20 50 52 – 93 62 62 – – – 7 7 3 0 20 8 0 0 0
Endoplasmic Reticulum – 17 6 3 – 3 1 2 – 3 1 5 – – – 7 1 1 0 19 25 0 15 5
Endosome – 0 0 0 – 0 0 0 – 0 0 0 – – – 0 0 0 0 13 9 0 0 0
Golgi – 0 4 2 – 2 1 1 – 0 1 0 – – – 5 5 3 0 43 79 0 0 0
Mitochondria – 4 3 5 – 1 2 0 – 0 2 1 – – – 7 3 2 0 0 1 0 0 0
Nucleus – 1 4 0 – 1 3 1 – 2 3 0 – – – 7 6 1 0 14 5 0 0 0
Nuclear Periphery – 0 0 0 – 0 0 0 – 0 0 0 – – – 0 0 0 0 1 0 0 0 0
Nucleolus – 40 39 23 – 18 22 24 – 11 17 6 – – – 24 5 4 0 4 1 0 0 0
Peroxisomes – 0 0 0 – 0 0 0 – 0 0 0 – – – 0 0 0 0 3 3 0 0 0
SpindlePole – 0 0 0 – 0 0 0 – 0 0 0 – – – 0 1 0 0 1 0 0 0 0
Vac/Vac Membrane – 5 20 3 – 14 21 9 – 5 4 1 – – – 61 20 13 2 66 36 0 1 0
Unique Cell Count 90 84 41 53 89 86 112 85 73 100 43 22 5 212 193 1 17 7
Labelled Cell Count 98 96 48 59 101 89 115 91 75 119 49 27 5 212 193 1 17 7


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 219.2 200.9 202.3 – 250.1 302.2 268.6 – 343.5 312.2 350.8 – – – 120.0 94.0 174.7 – – –
Std Deviation (1e-4) – 56.4 58.7 67.9 – 89.7 98.2 105.5 – 97.2 99.7 95.2 – – – 52.2 57.7 64.5 – – –
Intensity Change (Log2) – – – 0.01 – 0.32 0.59 0.42 – 0.77 0.64 0.8 – – – -0.74 -1.1 -0.2 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 – 0 0 0 – 0 0 0 – – – 0 0 0
Bud 0 – 0 0 0 – 0 0 0 – – – 0 0 0
Bud Neck 0 – 0 0 0 – 0 0 0 – – – 0 0 0
Bud Site 0 – 0 0 0 – 0 0 0 – – – 0 0 0
Cell Periphery 0 – 0 0 0 – 0 0 0 – – – 0 0 0
Cytoplasm 0.4 – 1.7 4.3 4.8 – 8.3 6.4 7.6 – – – -3.2 -1.0 0
Endoplasmic Reticulum 0 – 0 0 0 – 0 0 -0.1 – – – 0 0 0
Endosome 0 – 0 0 0 – 0 0 0 – – – 0 0 0
Golgi 0 – 0 0 0 – 0 0 0 – – – 0 0 0
Mitochondria 0 – 0 0 0 – 0 0 0 – – – 0 0 0
Nucleus 0 – 0 0 0 – 0 0 0 – – – 0.6 0 0
Nuclear Periphery 0 – 0 0 0 – 0 0 0 – – – 0 0 0
Nucleolus 1.0 – -1.4 -3.0 -2.5 – -5.8 -3.6 -5.3 – – – -3.2 -3.9 -2.4
Peroxisomes 0 – 0 0 0 – 0 0 0 – – – 0 0 0
SpindlePole 0 – 0 0 0 – 0 0 0 – – – 0 0 0
Vacuole -2.2 – 0.3 0 -2.3 – -4.0 -3.6 -4.1 – – – 5.1 2.6 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.8859 18.7686 19.0486 12.9947 10.2781 18.9405 14.7031 17.7536 13.563 9.5368 10.1705 17.5281 15.0466 18.4523 17.0799 13.5228 9.2288 16.3433
Actin 0.0078 0.0001 0.0027 0.0166 0.0696 0.0001 0.0112 0.0005 0.0016 0.0174 0.0843 0.0001 0.0002 0 0.0275 0 0 0.0001
Bud 0.0009 0.0004 0.001 0.0017 0.0044 0.0014 0.0006 0.0007 0.0004 0.0013 0.0008 0.0006 0 0.0003 0.0005 0.0002 0.0001 0.0003
Bud Neck 0.0017 0.0008 0.0041 0.0008 0.003 0.0021 0.0013 0.0019 0.0013 0.002 0.0018 0.0011 0.0001 0.0015 0.0009 0.0006 0.0003 0.0018
Bud Periphery 0.0015 0.0008 0.0017 0.003 0.0051 0.0027 0.0018 0.0021 0.0013 0.0046 0.0015 0.0022 0.0001 0.0009 0.0012 0.0004 0.0003 0.0013
Bud Site 0.0033 0.0011 0.0185 0.0024 0.0043 0.0013 0.0151 0.0023 0.0015 0.0019 0.0063 0.0008 0 0.0011 0.0011 0.0004 0.0001 0.0007
Cell Periphery 0.008 0.0087 0.0055 0.0039 0.0033 0.0068 0.0085 0.0179 0.0041 0.0071 0.0115 0.0082 0.0005 0.0191 0.0043 0.0026 0.0037 0.0043
Cytoplasm 0.041 0.0161 0.004 0.0249 0.01 0.0019 0.0152 0.0083 0.0023 0.0036 0.0015 0.0039 0.0255 0.0009 0.092 0.0011 0.0002 0.0625
Cytoplasmic Foci 0.0521 0.005 0.0103 0.033 0.089 0.009 0.0103 0.0082 0.0127 0.0202 0.0141 0.0022 0.0081 0.0028 0.0168 0.0031 0.002 0.0055
Eisosomes 0.0001 0 0.0001 0.0002 0.0004 0 0.0002 0 0.0001 0.0002 0.0007 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0103 0.0047 0.0051 0.0126 0.0028 0.0029 0.0721 0.0283 0.0107 0.0032 0.0039 0.0543 0.005 0.0011 0.0219 0.0005 0.0005 0.0202
Endosome 0.1202 0.0803 0.0952 0.1134 0.0739 0.0572 0.1067 0.0514 0.1302 0.1221 0.118 0.0886 0.3001 0.0732 0.0659 0.1122 0.0588 0.0763
Golgi 0.0204 0.0089 0.0104 0.0048 0.0147 0.0056 0.016 0.0066 0.0215 0.0103 0.0543 0.012 0.0065 0.0034 0.0301 0.0045 0.0035 0.0086
Lipid Particles 0.045 0.0101 0.0121 0.0213 0.0291 0.0153 0.0271 0.0333 0.024 0.0341 0.0299 0.0223 0.0022 0.0397 0.0223 0.0039 0.0918 0.0496
Mitochondria 0.0211 0.0049 0.0292 0.011 0.0066 0.0021 0.0198 0.0031 0.0302 0.0113 0.0118 0.0111 0.0007 0.0353 0.1157 0.0007 0.0005 0.0104
None 0.0081 0.0001 0.0002 0.0051 0.0307 0.0001 0.0052 0.0002 0.0002 0.0025 0.0006 0.0001 0.2923 0.0001 0.0003 0 0 0.0003
Nuclear Periphery 0.0421 0.0161 0.0155 0.01 0.0041 0.0048 0.0555 0.011 0.0119 0.0041 0.0037 0.046 0.0055 0.0035 0.0089 0.001 0.009 0.0156
Nucleolus 0.0075 0.0055 0.0158 0.0157 0.0024 0.009 0.0027 0.0088 0.0019 0.0044 0.0097 0.0045 0.0011 0.0097 0.0022 0.0022 0.0024 0.0201
Nucleus 0.0354 0.0072 0.0126 0.0073 0.0025 0.0059 0.0081 0.006 0.0024 0.0049 0.0033 0.0046 0.0053 0.0038 0.017 0.0016 0.0019 0.0391
Peroxisomes 0.0042 0.0002 0.0032 0.0008 0.0137 0.0008 0.0084 0.0002 0.0082 0.0037 0.0054 0.0002 0.0001 0.0004 0.0568 0.0002 0.0001 0.0009
Punctate Nuclear 0.0544 0.0001 0.0003 0.0021 0.0117 0.0005 0.0011 0.0001 0.0003 0.0011 0.0005 0.0001 0.0061 0.0001 0.0005 0 0.0001 0.0022
Vacuole 0.333 0.5948 0.495 0.5188 0.4619 0.6419 0.4653 0.6142 0.4931 0.565 0.5074 0.5635 0.1554 0.4673 0.284 0.6199 0.5668 0.4065
Vacuole Periphery 0.1819 0.2343 0.2577 0.1905 0.157 0.2286 0.1478 0.1949 0.2402 0.1749 0.1292 0.1735 0.1847 0.3357 0.23 0.2447 0.2578 0.2739

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.6758 41.4398 31.9639 33.6739 25.702 17.0692 14.4719 15.8142 20.88 18.257
Translational Efficiency 1.3635 1.0457 0.9591 1.123 1.2481 0.6845 1.0682 0.7465 0.8667 0.836

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1142 162 1856 97 51 104 1545 1045 1193 266 3401 1142

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1253.92 3758.05 2095.29 965.69 10330.82 2066.95 1225.55 956.66 1641.95 3096.87 1700.19 957.43
Standard Deviation 463.02 3567.35 786.99 200.89 3778.61 1436.68 391.74 186.85 2046.25 3039.47 771.52 188.10
Intensity Change Log 2 — 1.583539 0.740705 -0.376813 — -2.321379 -3.075454 -3.432805 — -0.991896 -1.802606 -2.591283

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001561 0.001460 0.009111 0.002455 0.000066 0.003285 0.026309 0.002320 0.001497 0.002174 0.016924 0.002331
Bud Neck 0.011091 0.010976 0.005887 0.032105 0.002046 0.010545 0.027536 0.016791 0.010704 0.010807 0.015721 0.018091
Bud Site 0.046472 0.080402 0.024131 0.085884 0.000781 0.024645 0.143246* 0.047358 0.044519 0.058602 0.078243 0.050630
Cell Periphery 0.004026 0.082573 0.006965 0.009194 0.076422 0.110565* 0.028114 0.001081 0.007121 0.093517 0.016573 0.001770
Cytoplasm 0.066946 0.030891 0.008447 0.214737* 0.000034 0.001489 0.137495* 0.251136* 0.064085 0.019396 0.067071 0.248044*
Cytoplasmic Foci 0.028151 0.014874 0.016901 0.057727 0.000682 0.008969 0.026091 0.058730 0.026977 0.012566 0.021076 0.058645
Eisosomes 0.000114 0.000378 0.000286 0.000198 0.001444 0.000463 0.001522 0.000089 0.000170 0.000412 0.000847 0.000098
Endoplasmic Reticulum 0.018109 0.009544 0.099853 0.005564 0.000926 0.014561 0.175510* 0.029405 0.017374 0.011505 0.134222* 0.027380
Endosome 0.100852 0.016302 0.121843 0.052589 0.000388 0.023025 0.061622 0.053461 0.096557 0.018931 0.094486 0.053387
Golgi 0.041880 0.016089 0.213649* 0.014146 0.001492 0.005337 0.022501 0.008444 0.040153 0.011885 0.126815* 0.008929
Lipid Particles 0.007456 0.008075 0.269970* 0.004665 0.001263 0.001398 0.117740* 0.011404 0.007192 0.005465 0.200816* 0.010831
Mitochondria 0.004029 0.001250 0.001738 0.019070 0.000065 0.001623 0.002706 0.004909 0.003859 0.001396 0.002178 0.006112
Mitotic Spindle 0.006763 0.000408 0.001839 0.004141 0.000004 0.000278 0.004148 0.014652 0.006474 0.000358 0.002888 0.013759
None 0.000497 0.000690 0.000634 0.000936 0.000034 0.000054 0.000875 0.002177 0.000477 0.000441 0.000743 0.002072
Nuclear Periphery 0.000934 0.000233 0.001452 0.000568 0.000116 0.000727 0.000475 0.001023 0.000899 0.000426 0.001008 0.000984
Nuclear Periphery Foci 0.005935 0.001471 0.017699 0.004131 0.000570 0.001594 0.009125 0.005462 0.005705 0.001519 0.013804 0.005349
Nucleolus 0.001061 0.001562 0.000061 0.000343 0.000754 0.000403 0.000167 0.000612 0.001048 0.001109 0.000109 0.000589
Nucleus 0.008389 0.006674 0.000796 0.017530 0.000059 0.001170 0.015178 0.040267 0.008033 0.004522 0.007330 0.038336
Peroxisomes 0.001898 0.000475 0.000190 0.003394 0.000006 0.000794 0.002469 0.005088 0.001817 0.000600 0.001225 0.004944
Vacuole 0.566512* 0.629611* 0.171577 0.466828* 0.823727* 0.752013* 0.190543 0.439097* 0.577508* 0.677468* 0.180193 0.441452*
Vacuole Periphery 0.077325 0.086059 0.026970 0.003796 0.089120 0.037061 0.006627 0.006494 0.077830 0.066902 0.017729 0.006265

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.17 -7.26 0.09 0.23 7.10 -1.39 -12.88 -4.50 0.71 11.89 -0.80 -13.72 -0.28 0.67 13.51
Bud Neck -0.19 3.88 -1.29 -1.16 -2.29 -3.33 -14.29 -7.97 -0.93 6.88 -0.22 -3.43 -1.69 -1.21 1.40
Bud Site -2.34 5.54 0.23 2.02 -1.69 -3.13 -25.59* -11.04 -1.17 17.29* -1.34 -7.28 2.63 2.57 10.74
Cell Periphery -6.45 -3.62 -0.04 6.18 0.85 -1.25 2.54 3.83 5.97 13.68 -8.29 -5.91 5.00 8.93 15.31
Cytoplasm 3.60 12.62 -5.97 -7.06 -8.41 -1.87 -27.26 -33.53 -33.17 -12.98 6.44 -0.18 -21.80 -25.34 -23.92
Cytoplasmic Foci 1.55 5.10 -2.15 -2.77 -3.74 -1.38 -15.91 -19.26 -4.34 -7.89 2.19 2.94 -6.94 -6.12 -10.95
Eisosomes -2.51 -4.84 -0.91 2.10 3.25 2.07 0.05 3.08 2.32 10.25 -2.55 -9.04 2.44 3.62 11.61
Endoplasmic Reticulum 2.69 -16.91 5.65 1.97 21.05 -2.85 -23.89 -5.99 -0.70 19.71 1.82 -25.33 -0.08 -1.67 23.56
Endosome 15.09 -4.63 7.67 -1.14 10.74 -2.78 -22.07 -13.33 -1.31 6.46 13.51 0.33 12.85 -3.25 18.64
Golgi 4.63 -28.26* 6.69 1.70 31.63* -1.59 -10.86 -2.29 0.15 7.57 6.54 -18.94 10.09 2.25 33.80*
Lipid Particles -0.19 -40.26* 2.98 1.25 41.06* -0.15 -20.96 -5.07 -5.47 19.31 0.80 -41.97 -0.67 -1.20 41.46*
Mitochondria 1.71 2.05 -0.54 -0.81 -0.81 -1.70 -6.89 -3.14 -1.04 -0.38 1.83 1.63 0.20 -1.37 -1.13
Mitotic Spindle 5.70 4.34 -0.17 -2.25 -1.80 -1.69 -4.45 -5.53 -5.39 -3.03 5.81 2.93 -2.22 -5.57 -4.09
None -1.06 -0.95 -2.00 -1.58 -1.78 -0.82 -5.75 -2.83 -2.79 -1.47 -0.07 -1.90 -2.30 -2.25 -1.83
Nuclear Periphery 4.05 -2.98 -0.71 -3.94 1.58 -1.12 -7.71 -8.49 -0.90 -5.45 1.71 -0.83 -1.74 -3.10 -1.49
Nuclear Periphery Foci 3.50 -7.54 1.05 -3.35 9.95 -1.08 -10.43 -5.72 -2.44 4.80 3.55 -6.54 1.09 -3.58 10.20
Nucleolus -0.85 3.10 1.78 1.83 -2.40 1.98 4.51 0.90 -1.31 -4.34 -0.28 3.03 1.41 1.40 -5.24
Nucleus -0.38 5.81 -4.58 -3.57 -6.05 -2.01 -13.69 -18.24 -17.58 -11.85 0.56 -0.30 -15.05 -12.96 -15.76
Peroxisomes 3.64 5.68 -0.57 -2.77 -3.47 -1.37 -13.56 -6.14 -3.20 -1.81 3.05 2.17 -2.54 -4.66 -4.13
Vacuole -2.08 38.01* 0.85 2.06 -11.26 1.89 20.73 11.83 10.19 -23.09 -4.63 40.10* 8.40 9.68 -28.85
Vacuole Periphery -0.78 12.75 19.95 7.02 19.62* 1.86 3.20 3.29 3.14 2.60 1.04 15.75 19.56 7.41 15.21
Standard name
Human Ortholog
Description General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth
Localization
Cell Percentages ER (9%), vacuole (8%), mixed (42%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Gap1

Gap1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gap1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available