Standard name
Human Ortholog
Description Cytoplasmic heavy chain dynein; microtubule motor protein; member of the AAA+ protein family, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p; motility along microtubules inhibited by She1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.87 0.91 0.93 0.9 0.89 0.89 0.87 0.81 0.78 0.66 0.71 0.9 0.92 0.96 0.83 0.84 0.88 0.7 0.72 0.66 0.8 0.74 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.05 0.08 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.1 0.1 0.2 0.25 0.32 0.46 0.53 0.53 0 0 0 0 0 0 0.06 0.1 0.16 0.08 0.1 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 5 3 0 0 0 0 0 0 0 0 2 1 0 8 12 7 8 13 13 10 15 15
Bud 3 5 12 14 12 7 18 22 17 19 11 2 2 7 2 2 3 1 6 6 1 6 10
Bud Neck 3 2 11 2 1 3 1 8 1 2 1 1 0 0 0 0 0 4 10 10 2 5 2
Bud Site 0 0 0 0 0 1 0 1 2 3 0 0 0 1 0 0 0
Cell Periphery 7 6 3 2 4 4 2 4 6 6 2 1 0 2 11 7 8 0 3 2 0 2 1
Cytoplasm 267 445 515 310 443 563 655 641 386 298 314 306 419 452 310 319 354 241 384 383 243 311 342
Endoplasmic Reticulum 1 3 4 0 0 5 1 3 1 2 1 4 1 0 22 16 14 3 0 6 0 5 2
Endosome 18 13 9 2 15 6 4 2 0 1 0 15 15 9 26 22 22 28 25 21 5 4 7
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 5 2 4 0 1 0
Mitochondria 4 16 10 35 48 128 187 256 229 240 236 1 4 9 6 5 8 22 51 90 23 43 26
Nucleus 0 2 0 0 0 1 2 7 2 1 4 1 0 0 3 4 4 0 1 0 0 0 0
Nuclear Periphery 0 2 0 0 1 1 2 0 0 1 0 1 0 3 0 0 2 1 1 1 0 0 0
Nucleolus 1 1 0 0 1 0 0 4 0 0 2 0 1 1 0 1 0 0 1 0 0 0 0
Peroxisomes 2 1 1 2 2 0 2 0 0 1 0 5 6 2 0 1 0 2 0 0 0 1 0
SpindlePole 10 5 6 9 11 4 16 9 3 5 5 11 13 4 17 10 6 16 11 15 4 6 5
Vac/Vac Membrane 8 3 2 1 6 0 5 6 2 6 3 0 4 4 18 24 16 4 13 10 4 7 6
Unique Cell Count 308 490 553 343 498 635 757 791 498 449 445 340 453 473 373 382 403 345 536 582 303 419 426
Labelled Cell Count 330 510 576 377 544 723 895 963 649 585 579 350 466 494 425 425 444 345 536 582 303 419 426


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 4.5 4.0 3.9 4.0 3.7 3.4 3.3 3.1 3.0 2.8 4.6 4.8 4.4 8.0 8.7 7.9 4.9 5.3 5.7
Std Deviation (1e-4) 0.8 0.9 1.0 1.4 1.2 1.2 1.1 1.0 1.3 1.1 0.9 0.8 1.0 1.0 1.5 1.7 1.8 1.0 1.2 1.3
Intensity Change (Log2) -0.06 0.01 -0.13 -0.23 -0.3 -0.38 -0.42 -0.54 0.2 0.25 0.13 1.0 1.12 0.98 0.29 0.39 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 4.6 5.4 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.8757 3.7191 3.4572 3.3317 3.9908 3.4614 -1.0832 -0.7265 -0.8304 -1.0447 -1.3681 -0.6023 3.7862 4.7724 4.3157 3.9462 3.9411 4.4509
Actin 0.0266 0.0026 0.0285 0.0061 0.0421 0.0049 0.0479 0.0014 0.0017 0.0017 0.0013 0.0042 0.0068 0.0047 0.0051 0.0075 0.0081 0.0062
Bud 0.0011 0.0031 0.0027 0.0006 0.0014 0.0003 0.0047 0.0139 0.0022 0.0041 0.0013 0.0078 0.0008 0.0038 0.0055 0.0066 0.0091 0.0013
Bud Neck 0.0135 0.0003 0.0078 0.0735 0.0008 0.0014 0.0058 0.0008 0.0175 0.0186 0.0257 0.0045 0.0006 0.0011 0.0189 0.0364 0.001 0.0049
Bud Periphery 0.0013 0.0053 0.0055 0.0009 0.0017 0.0004 0.0067 0.0061 0.0018 0.006 0.0025 0.003 0.0014 0.0024 0.0055 0.0076 0.0285 0.0017
Bud Site 0.0115 0.0121 0.0158 0.0074 0.002 0.0002 0.0075 0.0392 0.0121 0.0248 0.0005 0.0027 0.0036 0.0259 0.0166 0.036 0.002 0.0009
Cell Periphery 0.0007 0.0014 0.0011 0.0016 0.0003 0.0004 0.0006 0.0006 0.0002 0.0005 0.0001 0.0003 0.0004 0.0005 0.0006 0.0016 0.0006 0.0002
Cytoplasm 0.2987 0.5749 0.4921 0.3118 0.3731 0.5979 0.2771 0.4025 0.3673 0.3446 0.3626 0.4517 0.2904 0.4195 0.3013 0.2826 0.3332 0.3981
Cytoplasmic Foci 0.0415 0.0156 0.0465 0.1902 0.059 0.0405 0.083 0.0537 0.0604 0.1688 0.0906 0.0787 0.0541 0.0292 0.0759 0.163 0.099 0.0521
Eisosomes 0.0004 0.0001 0.0003 0.0005 0.0004 0.0001 0.0004 0.0001 0.0001 0.0001 0 0 0.0003 0.0001 0.0002 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0091 0.0061 0.005 0.0028 0.0046 0.0071 0.0069 0.0036 0.003 0.0029 0.0021 0.0035 0.0031 0.0021 0.0027 0.0024 0.0045 0.0029
Endosome 0.0196 0.0047 0.0097 0.0182 0.0462 0.0122 0.0377 0.0254 0.0305 0.0681 0.0731 0.0296 0.0138 0.013 0.0164 0.0151 0.0381 0.0146
Golgi 0.0053 0.0007 0.0026 0.0032 0.015 0.0013 0.006 0.0016 0.0016 0.0021 0.0031 0.0015 0.0015 0.0017 0.0016 0.0011 0.0025 0.0012
Lipid Particles 0.0064 0.0009 0.0063 0.0051 0.027 0.0043 0.0074 0.0014 0.0026 0.0015 0.0032 0.0035 0.0019 0.0006 0.0012 0.0005 0.0013 0.0007
Mitochondria 0.0027 0.0004 0.0042 0.0018 0.0417 0.0009 0.0065 0.0019 0.0021 0.0023 0.0038 0.0012 0.0009 0.0018 0.0014 0.0011 0.002 0.0016
None 0.4955 0.3642 0.3385 0.3568 0.3066 0.3178 0.4511 0.4249 0.4731 0.3267 0.4117 0.3807 0.611 0.4776 0.5279 0.4242 0.451 0.5
Nuclear Periphery 0.0323 0.0007 0.005 0.0009 0.0144 0.0028 0.0071 0.0017 0.0016 0.0017 0.0008 0.008 0.0009 0.0012 0.0015 0.001 0.0028 0.0012
Nucleolus 0.0019 0.0003 0.0029 0.0009 0.0011 0.0001 0.0029 0.001 0.0006 0.0008 0.0002 0.0006 0.0003 0.0008 0.0006 0.0008 0.0005 0.0004
Nucleus 0.0032 0.0013 0.0042 0.0019 0.003 0.0011 0.0112 0.0053 0.0034 0.0043 0.0019 0.0062 0.0012 0.0037 0.0023 0.003 0.0027 0.0023
Peroxisomes 0.0141 0.0012 0.0104 0.0083 0.044 0.0016 0.012 0.001 0.0019 0.0034 0.0041 0.0029 0.0024 0.0018 0.0068 0.0027 0.0035 0.0047
Punctate Nuclear 0.0112 0.0011 0.0073 0.0042 0.0119 0.0029 0.0064 0.0088 0.0125 0.0065 0.0018 0.0052 0.0021 0.0056 0.0058 0.0049 0.0035 0.0034
Vacuole 0.0021 0.0028 0.0031 0.003 0.0024 0.0013 0.0093 0.0044 0.0033 0.0079 0.0082 0.0035 0.002 0.0024 0.0019 0.0016 0.0048 0.0011
Vacuole Periphery 0.0013 0.0002 0.0005 0.0003 0.0013 0.0003 0.0017 0.0006 0.0006 0.0024 0.0012 0.0007 0.0003 0.0004 0.0004 0.0003 0.0011 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.4112 14.4595 17.2865 16.1989 13.3985 10.8672 25.1179 24.1205 19.9162 19.2341
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency