| Standard name | |
|---|---|
| Description | Cytoplasmic heavy chain dynein; microtubule motor protein; member of the AAA+ protein family, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p; motility along microtubules inhibited by She1p |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 5 | 5 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 1 | 0 | 8 | 12 | 7 | 8 | 13 | 13 | 10 | 15 | 15 |
| Bud | 3 | 5 | 12 | 14 | 12 | 7 | 18 | 22 | 17 | – | 19 | 11 | 2 | 2 | 7 | 2 | 2 | 3 | 1 | 6 | 6 | 1 | 6 | 10 |
| Bud Neck | 3 | 2 | 11 | 2 | 1 | 3 | 1 | 8 | 1 | – | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 10 | 10 | 2 | 5 | 2 |
| Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | – | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 7 | 6 | 3 | 2 | 4 | 4 | 2 | 4 | 6 | – | 6 | 2 | 1 | 0 | 2 | 11 | 7 | 8 | 0 | 3 | 2 | 0 | 2 | 1 |
| Cytoplasm | 267 | 445 | 515 | 310 | 443 | 563 | 655 | 641 | 386 | – | 298 | 314 | 306 | 419 | 452 | 310 | 319 | 354 | 241 | 384 | 383 | 243 | 311 | 342 |
| Endoplasmic Reticulum | 1 | 3 | 4 | 0 | 0 | 5 | 1 | 3 | 1 | – | 2 | 1 | 4 | 1 | 0 | 22 | 16 | 14 | 3 | 0 | 6 | 0 | 5 | 2 |
| Endosome | 18 | 13 | 9 | 2 | 15 | 6 | 4 | 2 | 0 | – | 1 | 0 | 15 | 15 | 9 | 26 | 22 | 22 | 28 | 25 | 21 | 5 | 4 | 7 |
| Golgi | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 5 | 2 | 4 | 0 | 1 | 0 |
| Mitochondria | 4 | 16 | 10 | 35 | 48 | 128 | 187 | 256 | 229 | – | 240 | 236 | 1 | 4 | 9 | 6 | 5 | 8 | 22 | 51 | 90 | 23 | 43 | 26 |
| Nucleus | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 7 | 2 | – | 1 | 4 | 1 | 0 | 0 | 3 | 4 | 4 | 0 | 1 | 0 | 0 | 0 | 0 |
| Nuclear Periphery | 0 | 2 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | – | 1 | 0 | 1 | 0 | 3 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 0 |
| Nucleolus | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 4 | 0 | – | 0 | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| Peroxisomes | 2 | 1 | 1 | 2 | 2 | 0 | 2 | 0 | 0 | – | 1 | 0 | 5 | 6 | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 0 |
| SpindlePole | 10 | 5 | 6 | 9 | 11 | 4 | 16 | 9 | 3 | – | 5 | 5 | 11 | 13 | 4 | 17 | 10 | 6 | 16 | 11 | 15 | 4 | 6 | 5 |
| Vac/Vac Membrane | 8 | 3 | 2 | 1 | 6 | 0 | 5 | 6 | 2 | – | 6 | 3 | 0 | 4 | 4 | 18 | 24 | 16 | 4 | 13 | 10 | 4 | 7 | 6 |
| Unique Cell Count | 308 | 490 | 553 | 343 | 498 | 635 | 757 | 791 | 498 | 449 | 445 | 340 | 453 | 473 | 373 | 382 | 403 | 345 | 536 | 582 | 303 | 419 | 426 | |
| Labelled Cell Count | 330 | 510 | 576 | 377 | 544 | 723 | 895 | 963 | 649 | 585 | 579 | 350 | 466 | 494 | 425 | 425 | 444 | 345 | 536 | 582 | 303 | 419 | 426 | |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 6.2 | 4.5 | 4.0 | 3.9 | 4.0 | 3.7 | 3.4 | 3.3 | 3.1 | – | 3.0 | 2.8 | 4.6 | 4.8 | 4.4 | 8.0 | 8.7 | 7.9 | 4.9 | 5.3 | 5.7 |
| Std Deviation (1e-4) | 0.8 | 0.9 | 1.0 | 1.4 | 1.2 | 1.2 | 1.1 | 1.0 | 1.3 | – | 1.1 | 0.9 | 0.8 | 1.0 | 1.0 | 1.5 | 1.7 | 1.8 | 1.0 | 1.2 | 1.3 |
| Intensity Change (Log2) | – | – | – | -0.06 | 0.01 | -0.13 | -0.23 | -0.3 | -0.38 | – | -0.42 | -0.54 | 0.2 | 0.25 | 0.13 | 1.0 | 1.12 | 0.98 | 0.29 | 0.39 | 0.51 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 4.6 | 5.4 | 4.1 |
External localization resources
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Dyn1-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
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Images are not yet available
Images are not yet available
