Standard name | |
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Description | Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 7 | 13 | 11 | 26 | 26 | 48 | 28 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 2 | 6 | 2 | 1 | 1 | 6 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 6 | 10 | 4 | 4 | 12 | 20 | 27 | 31 | 26 | 55 | 47 | 5 | 14 | 15 | 6 | 9 | 5 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 162 | 321 | 357 | 318 | 321 | 287 | 376 | 318 | 227 | 283 | 236 | 256 | 231 | 375 | 501 | 266 | 380 | 184 | 153 | 311 | 340 | 238 | 223 | 277 |
Endoplasmic Reticulum | 0 | 1 | 5 | 9 | 13 | 25 | 73 | 68 | 61 | 93 | 59 | 91 | 2 | 3 | 2 | 13 | 12 | 6 | 2 | 1 | 5 | 0 | 1 | 4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 8 | 6 | 6 | 12 | 8 | 0 | 1 | 0 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 0 | 0 | 1 | 1 | 11 | 28 | 16 | 32 | 29 | 38 | 44 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 2 | 0 | 5 | 2 | 2 | 1 | 0 | 2 | 3 | 2 | 0 | 0 | 3 | 1 | 3 | 0 |
Unique Cell Count | 163 | 322 | 364 | 328 | 338 | 330 | 486 | 421 | 336 | 424 | 380 | 421 | 235 | 382 | 505 | 273 | 387 | 186 | 157 | 319 | 355 | 248 | 232 | 292 |
Labelled Cell Count | 165 | 328 | 373 | 332 | 342 | 350 | 522 | 451 | 387 | 466 | 460 | 482 | 240 | 394 | 520 | 292 | 408 | 198 | 157 | 319 | 355 | 248 | 232 | 292 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 69.3 | 97.4 | 74.8 | 63.6 | 53.4 | 43.4 | 40.5 | 41.1 | 36.9 | 36.7 | 34.7 | 35.0 | 83.8 | 78.8 | 72.6 | 103.0 | 93.0 | 103.8 | 97.1 | 96.7 | 104.1 |
Std Deviation (1e-4) | 14.4 | 18.9 | 15.9 | 14.8 | 12.8 | 11.4 | 10.7 | 10.4 | 9.7 | 8.9 | 9.0 | 8.3 | 19.5 | 15.4 | 16.4 | 34.5 | 34.7 | 38.0 | 27.0 | 27.6 | 26.0 |
Intensity Change (Log2) | – | – | – | -0.23 | -0.48 | -0.78 | -0.89 | -0.86 | -1.02 | -1.03 | -1.11 | -1.09 | 0.16 | 0.08 | -0.04 | 0.46 | 0.32 | 0.47 | 0.38 | 0.37 | 0.48 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 3.1 | 3.1 | 5.4 | 4.8 | 7.0 | 5.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.4 | -1.5 | 0.7 | 1.1 | 2.4 | 3.6 | 2.3 | 5.7 | 4.5 | -0.5 | 0.7 | 0.2 | -0.4 | -0.4 | 0 |
Cytoplasm | -1.0 | -2.3 | -5.7 | -8.7 | -9.1 | -10.8 | -11.2 | -12.2 | -12.6 | 0.2 | 0.1 | 1.5 | -0.5 | 0.1 | 0.7 |
Endoplasmic Reticulum | 1.3 | 2.1 | 4.0 | 6.8 | 7.1 | 7.6 | 8.7 | 6.9 | 8.6 | 0 | 0 | 0 | 2.6 | 1.6 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.5 | 4.7 | 3.8 | 6.0 | 5.1 | 6.2 | 6.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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