Standard name
Description Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.08 0.06 0.13 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.06 0.09 0.06 0.14 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.98 0.97 0.95 0.87 0.77 0.76 0.68 0.67 0.62 0.61 0.98 0.98 0.99 0.97 0.98 0.99 0.98 0.98 0.96 0.96 0.96 0.95
Endoplasmic Reticulum 0 0 0 0 0 0.08 0.15 0.16 0.18 0.22 0.16 0.22 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.06 0 0.1 0.07 0.1 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 7 13 11 26 26 48 28 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 2 6 2 1 1 6 3 0 0 1 0 0 0 1 0 0 3 1 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 6 10 4 4 12 20 27 31 26 55 47 5 14 15 6 9 5 0 0 1 0 0 0
Cytoplasm 162 321 357 318 321 287 376 318 227 283 236 256 231 375 501 266 380 184 153 311 340 238 223 277
Endoplasmic Reticulum 0 1 5 9 13 25 73 68 61 93 59 91 2 3 2 13 12 6 2 1 5 0 1 4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 2 5 8 6 6 12 8 0 1 0 4 0 1 0 0 0 0 0 0
Mitochondria 1 0 0 1 1 11 28 16 32 29 38 44 0 0 0 1 2 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 2 1 0 1 1 1 1 0 0 1 0 1 0 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 1 1 0 1 2 0 5 2 2 1 0 2 3 2 0 0 3 1 3 0
Unique Cell Count 163 322 364 328 338 330 486 421 336 424 380 421 235 382 505 273 387 186 157 319 355 248 232 292
Labelled Cell Count 165 328 373 332 342 350 522 451 387 466 460 482 240 394 520 292 408 198 157 319 355 248 232 292


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 69.3 97.4 74.8 63.6 53.4 43.4 40.5 41.1 36.9 36.7 34.7 35.0 83.8 78.8 72.6 103.0 93.0 103.8 97.1 96.7 104.1
Std Deviation (1e-4) 14.4 18.9 15.9 14.8 12.8 11.4 10.7 10.4 9.7 8.9 9.0 8.3 19.5 15.4 16.4 34.5 34.7 38.0 27.0 27.6 26.0
Intensity Change (Log2) – – – -0.23 -0.48 -0.78 -0.89 -0.86 -1.02 -1.03 -1.11 -1.09 0.16 0.08 -0.04 0.46 0.32 0.47 0.38 0.37 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 3.1 3.1 5.4 4.8 7.0 5.0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.4 -1.5 0.7 1.1 2.4 3.6 2.3 5.7 4.5 -0.5 0.7 0.2 -0.4 -0.4 0
Cytoplasm -1.0 -2.3 -5.7 -8.7 -9.1 -10.8 -11.2 -12.2 -12.6 0.2 0.1 1.5 -0.5 0.1 0.7
Endoplasmic Reticulum 1.3 2.1 4.0 6.8 7.1 7.6 8.7 6.9 8.6 0 0 0 2.6 1.6 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 3.4 0 0 0 0 0 0 0
Mitochondria 0 0 3.5 4.7 3.8 6.0 5.1 6.2 6.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tif1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available