Standard name
Human Ortholog
Description Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.08 0.06 0.13 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0.06 0.09 0.06 0.14 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.98 0.97 0.95 0.87 0.77 0.76 0.68 0.67 0.62 0.61 0.98 0.98 0.99 0.97 0.98 0.99 0.98 0.98 0.96 0.96 0.96 0.95
Endoplasmic Reticulum 0 0 0 0 0 0.08 0.15 0.16 0.18 0.22 0.16 0.22 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.06 0 0.1 0.07 0.1 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 7 13 11 26 26 48 28 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 2 6 2 1 1 6 3 0 0 1 0 0 0 1 0 0 3 1 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 6 10 4 4 12 20 27 31 26 55 47 5 14 15 6 9 5 0 0 1 0 0 0
Cytoplasm 162 321 357 318 321 287 376 318 227 283 236 256 231 375 501 266 380 184 153 311 340 238 223 277
Endoplasmic Reticulum 0 1 5 9 13 25 73 68 61 93 59 91 2 3 2 13 12 6 2 1 5 0 1 4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 2 5 8 6 6 12 8 0 1 0 4 0 1 0 0 0 0 0 0
Mitochondria 1 0 0 1 1 11 28 16 32 29 38 44 0 0 0 1 2 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 2 1 0 1 1 1 1 0 0 1 0 1 0 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 1 1 0 1 2 0 5 2 2 1 0 2 3 2 0 0 3 1 3 0
Unique Cell Count 163 322 364 328 338 330 486 421 336 424 380 421 235 382 505 273 387 186 157 319 355 248 232 292
Labelled Cell Count 165 328 373 332 342 350 522 451 387 466 460 482 240 394 520 292 408 198 157 319 355 248 232 292


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 69.3 97.4 74.8 63.6 53.4 43.4 40.5 41.1 36.9 36.7 34.7 35.0 83.8 78.8 72.6 103.0 93.0 103.8 97.1 96.7 104.1
Std Deviation (1e-4) 14.4 18.9 15.9 14.8 12.8 11.4 10.7 10.4 9.7 8.9 9.0 8.3 19.5 15.4 16.4 34.5 34.7 38.0 27.0 27.6 26.0
Intensity Change (Log2) -0.23 -0.48 -0.78 -0.89 -0.86 -1.02 -1.03 -1.11 -1.09 0.16 0.08 -0.04 0.46 0.32 0.47 0.38 0.37 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 3.1 3.1 5.4 4.8 7.0 5.0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.4 -1.5 0.7 1.1 2.4 3.6 2.3 5.7 4.5 -0.5 0.7 0.2 -0.4 -0.4 0
Cytoplasm -1.0 -2.3 -5.7 -8.7 -9.1 -10.8 -11.2 -12.2 -12.6 0.2 0.1 1.5 -0.5 0.1 0.7
Endoplasmic Reticulum 1.3 2.1 4.0 6.8 7.1 7.6 8.7 6.9 8.6 0 0 0 2.6 1.6 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 3.4 0 0 0 0 0 0 0
Mitochondria 0 0 3.5 4.7 3.8 6.0 5.1 6.2 6.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 59.948 85.5053 75.4831 75.7662 73.7266 73.9282 48.4097 94.6967 79.3715 74.2537 74.6811 80.8133 78.1878 98.9593 91.9049 84.5921 82.9739 91.5759
Actin 0.0466 0.0003 0.014 0.0002 0.0016 0.0014 0.0145 0.002 0.0178 0.0097 0.0005 0.0032 0.0075 0.0001 0.0002 0.0009 0.0002 0.0003
Bud 0.0009 0.0003 0.0007 0.0008 0.0003 0.0009 0.0032 0.0005 0.0017 0.0021 0.0014 0.0015 0.0013 0.0002 0.0007 0.0003 0.0017 0.0004
Bud Neck 0.001 0.0003 0.0016 0.0009 0.0002 0.0013 0.0016 0.0006 0.001 0.0009 0.0009 0.0018 0.0007 0.0003 0.0006 0.0006 0.0008 0.0018
Bud Periphery 0.0008 0.0001 0.0004 0.0005 0.0001 0.0007 0.0039 0.0004 0.0008 0.0012 0.0005 0.0021 0.0015 0.0001 0.001 0.0002 0.0017 0.0003
Bud Site 0.0017 0.0003 0.0077 0.0002 0.0001 0.0001 0.0092 0.0013 0.0061 0.0035 0.0006 0.0006 0.001 0.0002 0.0003 0.0003 0.0002 0.0001
Cell Periphery 0.0057 0.0039 0.0096 0.0018 0.0002 0.002 0.0058 0.0117 0.0073 0.0012 0.006 0.0047 0.0071 0.0042 0.0062 0.0021 0.0014 0.0011
Cytoplasm 0.6558 0.9585 0.8349 0.827 0.8821 0.8571 0.6518 0.9338 0.8308 0.7883 0.8177 0.797 0.7715 0.9456 0.8887 0.7967 0.8571 0.8518
Cytoplasmic Foci 0.036 0.0009 0.0107 0.0023 0.0024 0.0055 0.0318 0.0031 0.0101 0.0199 0.0112 0.0043 0.0082 0.0006 0.0013 0.0091 0.0109 0.0038
Eisosomes 0.0017 0 0.0005 0 0 0 0.0008 0.0001 0.0004 0.0008 0.0001 0.0003 0.0002 0 0.0001 0.0011 0.0002 0
Endoplasmic Reticulum 0.0586 0.0102 0.0158 0.0132 0.0018 0.021 0.0388 0.0054 0.0133 0.0032 0.0028 0.0123 0.0636 0.0186 0.034 0.0152 0.0285 0.09
Endosome 0.013 0.0017 0.0093 0.0096 0.0061 0.0247 0.0387 0.0018 0.0231 0.0151 0.0096 0.025 0.0072 0.001 0.0025 0.011 0.0114 0.0093
Golgi 0.0041 0.0002 0.003 0.0004 0.0002 0.0118 0.0069 0.0009 0.0093 0.023 0.0004 0.0052 0.0013 0.0001 0.0004 0.0013 0.0012 0.0014
Lipid Particles 0.0132 0.0005 0.0074 0.0004 0.0001 0.0004 0.0122 0.0024 0.0034 0.0039 0.0042 0.001 0.0033 0.0006 0.0015 0.0121 0.0025 0.0005
Mitochondria 0.008 0.0003 0.0034 0.0022 0.0011 0.0206 0.0141 0.0012 0.002 0.0324 0.0012 0.0583 0.0076 0.0001 0.0069 0.0091 0.0079 0.0016
None 0.0144 0.0001 0.0029 0.0003 0.001 0.0004 0.022 0.0003 0.0049 0.0148 0.0122 0.001 0.0066 0.0001 0.0021 0.0105 0.0068 0.0012
Nuclear Periphery 0.021 0.0021 0.0037 0.0039 0.029 0.0049 0.0337 0.0024 0.0042 0.0037 0.0034 0.0389 0.0161 0.0019 0.0045 0.0118 0.0025 0.0051
Nucleolus 0.0092 0 0.0036 0.0001 0.0011 0.0002 0.0032 0.0002 0.0024 0.0028 0.0015 0.0011 0.0038 0 0.0013 0.0057 0.0119 0.0002
Nucleus 0.0161 0.0011 0.0026 0.0025 0.0443 0.0028 0.02 0.002 0.004 0.0043 0.0028 0.0126 0.0099 0.001 0.0029 0.0032 0.0092 0.0017
Peroxisomes 0.0065 0 0.0033 0.0001 0.0001 0.0001 0.0015 0.0003 0.0007 0.0021 0.0002 0.0002 0.0011 0 0.0001 0.0011 0.0004 0.0001
Punctate Nuclear 0.0159 0 0.0049 0 0.006 0.0001 0.0032 0.0002 0.0006 0.0008 0.0004 0.0003 0.0057 0 0.0001 0.0025 0.0006 0.0001
Vacuole 0.0525 0.0179 0.0529 0.1153 0.0192 0.0354 0.0716 0.0279 0.0509 0.0576 0.1062 0.0155 0.0635 0.0244 0.0405 0.0955 0.0327 0.022
Vacuole Periphery 0.0174 0.0014 0.007 0.0183 0.0028 0.0085 0.0114 0.0016 0.0051 0.0088 0.0165 0.0132 0.0112 0.0009 0.004 0.0097 0.0102 0.0072

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1836.221 1792.5809 1790.4034 1564.4865 1445.6648 1578.0716 1590.8492 1370.6169 1328.2719 1375.5069
Translational Efficiency 0.796 0.8362 0.7799 0.862 0.8911 0.9121 0.8566 0.9356 0.8468 0.8545

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1397 248 240 819 2071 1112 187 463 3468 1360 427 1282

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 4472.54 5944.49 6078.33 7245.03 4998.57 5229.26 7126.30 7848.72 4786.67 5359.68 6537.28 7463.06
Standard Deviation 1301.86 1145.17 1163.66 1729.82 1022.74 1054.52 1393.23 1555.12 1172.15 1106.64 1371.67 1693.84
Intensity Change Log 2 0.410459 0.442581 0.695897 0.065091 0.511638 0.650942 0.238508 0.479438 0.672346

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000046 0.000204 0.000136 0.000331 0.000040 0.000135 0.000247 0.000262 0.000043 0.000147 0.000185 0.000306
Bud Neck 0.000236 0.001165 0.005386 0.003265 0.000273 0.000802 0.005500 0.002216 0.000258 0.000868 0.005436 0.002886
Bud Site 0.002445 0.009399 0.005774 0.012834 0.002769 0.003492 0.014794 0.009911 0.002638 0.004569 0.009724 0.011778
Cell Periphery 0.014310 0.055221 0.027497 0.064398 0.007907 0.014791 0.030519 0.097885 0.010486 0.022163 0.028820 0.076492
Cytoplasm 0.811097* 0.596700* 0.336263* 0.469181* 0.809649* 0.742388* 0.439022* 0.359719* 0.810233* 0.715821* 0.381265* 0.429648*
Cytoplasmic Foci 0.006619 0.016828 0.014763 0.006538 0.011752 0.011718 0.014758 0.003698 0.009684 0.012650 0.014761 0.005512
Eisosomes 0.000001 0.000005 0.000005 0.000007 0.000001 0.000002 0.000007 0.000009 0.000001 0.000003 0.000006 0.000008
Endoplasmic Reticulum 0.000893 0.000780 0.000278 0.000325 0.001128 0.000728 0.000303 0.000280 0.001033 0.000738 0.000289 0.000309
Endosome 0.000465 0.001289 0.001510 0.002504 0.000623 0.000776 0.003141 0.000517 0.000560 0.000870 0.002224 0.001786
Golgi 0.038090 0.074694 0.033775 0.013555 0.046607 0.055215 0.025205 0.011384 0.043176 0.058767 0.030022 0.012771
Lipid Particles 0.010679 0.036867 0.038254 0.036535 0.008005 0.014003 0.054473 0.062822 0.009082 0.018172 0.045357 0.046029
Mitochondria 0.000115 0.000405 0.000074 0.001946 0.000235 0.000464 0.000353 0.000162 0.000186 0.000453 0.000196 0.001302
Mitotic Spindle 0.000524 0.001846 0.003496 0.001231 0.000999 0.001616 0.004965 0.000390 0.000808 0.001658 0.004139 0.000927
None 0.004638 0.001380 0.000646 0.001095 0.002295 0.001816 0.000751 0.000412 0.003239 0.001736 0.000692 0.000848
Nuclear Periphery 0.000367 0.000774 0.001334 0.000546 0.000393 0.000513 0.001365 0.000993 0.000383 0.000561 0.001348 0.000707
Nuclear Periphery Foci 0.008528 0.011603 0.015777 0.006039 0.008803 0.011099 0.014150 0.006865 0.008692 0.011191 0.015065 0.006337
Nucleolus 0.000079 0.000073 0.000128 0.000280 0.000043 0.000171 0.000125 0.000175 0.000057 0.000153 0.000126 0.000242
Nucleus 0.008670 0.007484 0.014869 0.011197 0.003700 0.011806 0.020494 0.006832 0.005702 0.011018 0.017333 0.009621
Peroxisomes 0.000031 0.000064 0.000032 0.000165 0.000020 0.000029 0.000059 0.000107 0.000024 0.000035 0.000044 0.000144
Vacuole 0.051030 0.123362 0.457961* 0.339125* 0.036074 0.083002 0.341144* 0.415120* 0.042098 0.090362 0.406802* 0.366571*
Vacuole Periphery 0.041136 0.059855 0.042043 0.028903 0.058687 0.045436 0.028625 0.020243 0.051617 0.048066 0.036167 0.025775

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.27 -6.77 -5.40 -2.13 -2.98 -2.86 -6.09 -3.38 -1.58 0.61 -3.65 -8.64 -6.40 -3.24 -1.85
Bud Neck -4.90 -7.36 -5.69 -4.01 3.22 -5.30 -6.91 -4.87 -3.44 4.76 -7.35 -10.08 -6.97 -5.40 5.19
Bud Site -5.96 -4.04 -5.65 -2.24 -3.40 -1.54 -2.73 -3.43 -3.00 1.18 -4.34 -3.68 -6.51 -5.16 -0.86
Cell Periphery -7.33 -2.79 -11.07 -0.11 -6.52 -5.10 -7.26 -15.44 -13.88 -10.45 -7.36 -6.76 -18.88 -13.80 -11.39
Cytoplasm 9.22 21.37 24.19 4.92 -5.56 6.50 15.44 26.51 20.37 2.32 9.96 26.11 35.18 21.26 -2.97
Cytoplasmic Foci -4.85 -4.79 -0.34 4.27 4.33 -0.42 -2.44 7.32 6.87 5.85 -3.62 -4.81 4.34 6.52 6.66
Eisosomes -4.67 -7.42 -8.16 -2.19 -1.13 -4.20 -6.99 -8.60 -7.10 0.09 -5.99 -10.04 -11.67 -8.24 -0.54
Endoplasmic Reticulum 0.77 5.63 5.47 3.76 -0.62 2.17 8.35 8.36 2.83 0.10 1.99 10.21 10.15 3.35 -0.41
Endosome -3.16 -8.16 -2.80 -1.39 -0.90 -2.20 -1.44 1.24 2.72 1.53 -4.15 -2.47 -2.58 -1.70 0.64
Golgi -5.55 2.63 10.90 9.81 6.29 -2.30 8.03 15.61 14.35 4.47 -5.07 7.48 18.39 16.93 7.55
Lipid Particles -6.70 -7.72 -9.96 0.48 0.78 -4.68 -7.25 -12.72 -11.00 -1.19 -7.12 -10.51 -16.24 -10.80 -0.00
Mitochondria -1.07 1.58 -1.36 -1.10 -1.38 -0.99 -0.29 0.90 1.27 0.67 -1.37 0.11 -1.28 -0.91 -1.29
Mitotic Spindle -5.89 -2.04 -2.17 1.57 1.62 -2.33 -1.48 4.69 4.67 1.76 -3.91 -2.44 -0.51 2.55 2.34
None 3.70 4.57 3.83 0.73 -2.17 0.77 2.51 3.00 9.21 2.84 2.88 4.99 4.37 4.27 -1.42
Nuclear Periphery -4.79 -6.92 -3.33 2.76 5.81 -2.20 -6.02 -2.30 -1.77 1.77 -4.16 -9.06 -3.17 -1.16 5.37
Nuclear Periphery Foci -2.50 -5.42 3.24 4.69 7.47 -2.62 -3.37 2.83 5.41 4.98 -3.50 -6.25 4.38 7.16 8.93
Nucleolus 0.24 -3.22 -3.77 -3.82 -2.42 -1.29 -7.76 -4.98 -0.03 -1.15 -1.19 -6.38 -5.00 -1.26 -2.67
Nucleus 0.32 -3.90 -2.97 -3.12 2.03 -7.51 -5.89 -3.05 1.11 3.83 -5.71 -7.27 -5.24 -0.75 4.00
Peroxisomes -2.11 -0.70 -3.01 -2.01 -2.82 -1.00 -2.47 -5.86 -4.34 -2.31 -1.36 -2.65 -4.22 -3.61 -3.27
Vacuole -6.23 -19.84 -28.13 -14.19 4.92 -9.95 -15.17 -28.47 -23.35 -2.75 -10.95 -24.81 -40.06 -30.00 2.30
Vacuole Periphery -2.78 1.16 4.94 5.26 2.26 3.78 8.87 12.40 7.73 1.48 1.40 6.66 12.23 8.40 2.50