Standard name
Description Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.05 0.15 0.13 0.18 0.16 0.15 0.21 0.11 – 0.13 0.11 0.09 0.15 0.21 0.1 0.1 0.11 0 0 0.06 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0.14 0.1 0.06 0 0.05 0.06 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.39 0.43 0.08 0.22 0.13 0.47 0.55 0.36 0.73 – 0.74 0.62 0.2 0.07 0.05 0.15 0.06 0.13 0 0 0 0 0.05 0.07
Nucleus 0.65 0.68 0.67 0.69 0.6 0.49 0.48 0.47 0.38 – 0.37 0.43 0.69 0.6 0.52 0.68 0.69 0.7 0.52 0.65 0.41 0.5 0.4 0.37
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.1 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0.11 0 0 0 0.13 0.06 0.06 0.07 0.09 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.09 0.06 0.12 0.07 0.05 0.09 0.07 – 0.06 0.08 0.05 0 0.06 0 0 0 0.07 0.05 0.1 0.06 0.07 0.07
Vac/Vac Membrane 0 0.08 0 0 0 0 0 0 0 – 0 0 0 0 0.08 0.1 0.14 0.11 0.08 0.11 0.21 0.24 0.27 0.28
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 1 2 0 – 0 0 0 0 0 0 0 0 0 0 1 1 1 1
Bud 1 0 2 0 1 4 12 6 12 – 16 9 1 1 1 0 0 0 1 1 5 3 8 10
Bud Neck 0 0 17 4 10 6 12 15 12 – 8 14 0 1 0 1 0 0 0 1 5 3 4 7
Bud Site 0 0 1 3 1 9 9 3 17 – 5 19 1 2 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 7 6 42 35 58 66 88 94 50 – 51 42 29 50 88 20 17 17 3 1 22 2 5 7
Endoplasmic Reticulum 1 0 1 0 1 0 1 0 1 – 0 0 1 0 0 12 7 9 0 0 2 3 1 3
Endosome 2 0 4 5 9 8 4 6 3 – 1 4 17 49 44 11 6 8 6 5 11 8 17 16
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 2 4
Mitochondria 37 51 23 59 42 198 326 159 321 – 299 246 64 25 22 31 11 20 4 4 13 6 24 29
Nucleus 62 80 186 182 195 209 286 204 164 – 150 171 218 202 220 136 122 107 58 84 170 148 199 165
Nuclear Periphery 1 0 0 0 1 1 0 1 0 – 0 0 1 2 1 0 0 0 3 0 3 1 4 2
Nucleolus 10 5 0 3 1 3 5 0 5 – 5 0 15 13 45 3 3 1 14 8 24 21 44 23
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 25 16 40 30 29 39 31 – 24 33 16 13 27 6 4 4 8 6 40 16 34 31
Vac/Vac Membrane 0 9 9 6 12 13 11 13 9 – 8 10 9 13 32 21 25 17 8 13 88 71 131 126
Unique Cell Count 96 118 276 265 324 424 598 438 437 403 399 317 339 422 200 176 152 113 130 413 296 494 448
Labelled Cell Count 123 151 311 313 371 548 784 542 625 567 548 372 371 482 242 195 184 113 130 413 296 494 448


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.2 5.5 4.6 5.1 4.0 4.2 4.5 3.6 – 3.4 3.7 4.5 4.7 4.8 6.8 6.8 7.2 4.3 4.3 4.9
Std Deviation (1e-4) 0.8 0.6 2.9 1.0 1.5 1.1 1.4 1.4 1.4 – 1.3 0.8 1.1 1.3 1.4 1.5 1.6 2.3 1.3 1.2 1.7
Intensity Change (Log2) – – – -0.27 -0.09 -0.47 -0.38 -0.29 -0.61 – -0.7 -0.55 -0.3 -0.23 -0.2 0.3 0.3 0.39 -0.36 -0.36 -0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.2 4.3 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6603 1.2767 1.4647 1.2056 0.8956 1.1409 -0.3336 -0.2674 -0.4083 -0.1876 -0.3318 -0.4233 0.8036 0.2784 0.8243 0.7201 0.3626 0.759
Actin 0.0519 0.0006 0.004 0.0009 0.0008 0.0006 0.0025 0.0003 0.0046 0.0164 0.0017 0.0024 0.0003 0 0.0001 0.0001 0 0
Bud 0.0004 0.0001 0.0003 0.0001 0.0001 0.0001 0.0005 0.0001 0.0004 0.0008 0.0013 0.0001 0 0 0 0 0 0
Bud Neck 0.0118 0.0014 0.002 0.0018 0.0028 0.0059 0.0015 0.0009 0.0009 0.0014 0.0012 0.0077 0.0007 0.0008 0.0004 0.0006 0.0004 0.0019
Bud Periphery 0.001 0.0002 0.0009 0.0002 0.0001 0.0001 0.0011 0.0001 0.0005 0.0023 0.0032 0.0003 0 0 0 0 0 0
Bud Site 0.01 0.0027 0.0032 0.0004 0.0002 0.0003 0.0016 0.0006 0.0051 0.0025 0.0028 0.0004 0.0002 0.0002 0.0001 0 0 0
Cell Periphery 0.0018 0.0001 0.0022 0.0001 0 0.0001 0.0003 0 0.0001 0.0002 0.0003 0.0001 0 0 0 0 0 0
Cytoplasm 0.0401 0.0185 0.0316 0.0153 0.0219 0.024 0.0261 0.0277 0.0184 0.019 0.0317 0.0118 0.0039 0.0039 0.0034 0.003 0.0011 0.0041
Cytoplasmic Foci 0.0282 0.0025 0.0055 0.001 0.0024 0.0027 0.0117 0.0007 0.0025 0.0141 0.0086 0.003 0.0017 0.0008 0.0006 0.0004 0.0002 0.0005
Eisosomes 0.001 0 0.0005 0.0001 0.0001 0.0002 0.0001 0 0.0001 0.0002 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0097 0.0023 0.002 0.0006 0.0011 0.0012 0.0068 0.0019 0.0027 0.0013 0.0055 0.0007 0.0003 0.0001 0.0001 0.0002 0 0
Endosome 0.0303 0.003 0.0054 0.0019 0.0028 0.0043 0.0411 0.0022 0.0052 0.0093 0.0663 0.0089 0.0003 0 0.0001 0.0002 0.0001 0.0001
Golgi 0.0078 0.0001 0.0011 0.0002 0.0001 0.0003 0.0083 0.0001 0.0015 0.0083 0.0298 0.0061 0.0001 0 0 0 0 0
Lipid Particles 0.025 0.0006 0.0039 0.0004 0.0004 0.002 0.0132 0.0002 0.0016 0.0223 0.0082 0.0095 0.0001 0 0 0 0 0
Mitochondria 0.0237 0.0006 0.0089 0.0004 0.0009 0.0006 0.0089 0.0004 0.0095 0.0099 0.0644 0.018 0.0002 0 0.0001 0 0.0001 0.0001
None 0.0308 0.028 0.2006 0.1391 0.0564 0.0849 0.0353 0.0146 0.0267 0.0594 0.0399 0.0215 0.0107 0.0126 0.0167 0.003 0.0011 0.0385
Nuclear Periphery 0.0303 0.0792 0.0497 0.0279 0.0362 0.0213 0.0452 0.0425 0.0644 0.0248 0.0818 0.0268 0.0053 0.0076 0.0101 0.0106 0.0047 0.003
Nucleolus 0.0214 0.0355 0.0322 0.0821 0.032 0.0493 0.0204 0.0179 0.0224 0.0196 0.0194 0.0364 0.0238 0.0171 0.0187 0.0148 0.0208 0.0199
Nucleus 0.5918 0.7397 0.5152 0.5988 0.7442 0.7279 0.7043 0.8388 0.7709 0.7141 0.5508 0.808 0.5942 0.4638 0.4242 0.5556 0.4076 0.4265
Peroxisomes 0.0143 0.0001 0.008 0.0002 0.0003 0.0007 0.0028 0.0001 0.0009 0.0224 0.0017 0.0059 0 0 0 0 0 0
Punctate Nuclear 0.0619 0.0813 0.12 0.1277 0.0962 0.0718 0.0499 0.049 0.0587 0.0492 0.0693 0.0309 0.3579 0.4928 0.5251 0.4111 0.5637 0.5052
Vacuole 0.0045 0.0025 0.0022 0.0006 0.0007 0.0012 0.012 0.0014 0.0017 0.0014 0.0072 0.0008 0.0001 0 0 0 0 0
Vacuole Periphery 0.0022 0.0008 0.0008 0.0002 0.0004 0.0003 0.0063 0.0006 0.0012 0.0012 0.0049 0.0006 0 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.7709 11.4164 11.0129 11.397 11.5906 10.2111 12.986 12.5952 12.4036 14.9825
Translational Efficiency 0.2953 0.2474 0.32 0.2896 0.3361 0.3236 0.3975 0.4284 0.3428 0.3147

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘