Standard name | |
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Description | Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 4 | 3 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 1 | – | 2 | 2 | 0 | 3 | 13 | 16 |
Bud | 0 | 3 | 1 | 4 | 2 | 10 | 7 | – | 11 | 7 | 3 | 7 | 0 | 2 | 1 | 0 | 0 | – | 0 | 1 | 0 | 0 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 0 | 2 | – | 1 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 1 | 7 | 4 | 11 | 8 | 1 | 6 | – | 0 | 5 | 6 | 3 | 2 | 9 | 4 | 2 | 1 | – | 0 | 0 | 0 | 0 | 0 | 3 |
Cytoplasm | 2 | 0 | 3 | 7 | 7 | 13 | 17 | – | 6 | 12 | 6 | 13 | 1 | 8 | 9 | 5 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 5 | 0 | 1 | 0 | 2 | 1 | – | 1 | 3 | 1 | 2 | 0 | 0 | 1 | 3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 3 | 2 | 5 | 4 | 17 | 4 | 16 | – | 3 | 11 | 1 | 3 | 2 | 4 | 6 | 0 | 0 | – | 4 | 4 | 2 | 0 | 5 | 4 |
Golgi | 3 | 5 | 53 | 18 | 44 | 5 | 16 | – | 5 | 8 | 5 | 2 | 12 | 28 | 54 | 22 | 10 | – | 11 | 13 | 6 | 12 | 18 | 25 |
Mitochondria | 168 | 168 | 159 | 175 | 144 | 300 | 253 | – | 237 | 316 | 257 | 250 | 129 | 258 | 236 | 6 | 1 | – | 158 | 167 | 190 | 133 | 282 | 238 |
Nucleus | 1 | 1 | 0 | 0 | 2 | 7 | 2 | – | 3 | 4 | 1 | 0 | 0 | 1 | 0 | 3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 17 | 5 | 7 | 4 | 4 | 24 | 15 | – | 14 | 24 | 16 | 19 | 9 | 7 | 7 | 1 | 1 | – | 1 | 1 | 0 | 2 | 3 | 6 |
Nucleolus | 15 | 6 | 6 | 3 | 11 | 36 | 32 | – | 27 | 37 | 25 | 14 | 4 | 6 | 11 | 4 | 1 | – | 2 | 2 | 1 | 2 | 6 | 5 |
Peroxisomes | 4 | 4 | 9 | 14 | 18 | 1 | 19 | – | 1 | 16 | 4 | 5 | 4 | 8 | 4 | 3 | 1 | – | 6 | 3 | 9 | 9 | 23 | 19 |
SpindlePole | 1 | 0 | 1 | 0 | 6 | 1 | 6 | – | 1 | 8 | 2 | 1 | 1 | 1 | 1 | 0 | 0 | – | 1 | 1 | 1 | 1 | 1 | 2 |
Vac/Vac Membrane | 18 | 36 | 21 | 21 | 58 | 35 | 43 | – | 24 | 30 | 15 | 24 | 17 | 28 | 32 | 12 | 4 | – | 2 | 10 | 2 | 3 | 5 | 10 |
Unique Cell Count | 187 | 191 | 197 | 204 | 227 | 361 | 354 | 269 | 393 | 289 | 293 | 143 | 282 | 276 | 56 | 17 | 196 | 212 | 216 | 176 | 367 | 344 | ||
Labelled Cell Count | 233 | 243 | 273 | 265 | 322 | 439 | 435 | 334 | 484 | 344 | 343 | 181 | 362 | 366 | 63 | 20 | 196 | 212 | 216 | 176 | 367 | 344 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.9 | 10.9 | 11.1 | 10.9 | 13.1 | 9.3 | 12.1 | – | 10.4 | 11.6 | 11.2 | 11.2 | 12.3 | 12.7 | 13.2 | 22.6 | 22.2 | – | 11.0 | 12.2 | 12.5 |
Std Deviation (1e-4) | 2.7 | 2.9 | 2.3 | 2.3 | 7.6 | 2.5 | 3.2 | – | 4.1 | 5.2 | 3.5 | 5.5 | 2.6 | 3.3 | 3.4 | 7.5 | 6.1 | – | 3.2 | 3.1 | 3.0 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.24 | -0.25 | 0.13 | – | -0.09 | 0.07 | 0.02 | 0.01 | 0.15 | 0.19 | 0.26 | 1.03 | 1.0 | – | -0.01 | 0.14 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud | 0 | 0 | 0 | 0 | – | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cell Periphery | 1.8 | 0.9 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0.8 | 0 | 0 | 0 | – |
Cytoplasm | 0 | 0 | 1.4 | 2.0 | – | 0 | 1.1 | 0 | 1.8 | 0 | 0 | 0 | 0 | 0 | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Endosome | 0 | 2.3 | 0 | 1.2 | – | 0 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Golgi | -4.7 | -1.8 | -9.4 | -7.6 | – | -8.1 | -9.4 | -8.4 | -9.0 | -4.3 | -4.9 | -1.9 | 1.8 | 2.8 | – |
Mitochondria | 1.4 | -3.9 | 0.7 | -2.4 | – | 2.2 | -0.1 | 2.5 | 1.3 | 2.4 | 3.5 | 1.4 | -9.7 | -6.8 | – |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nuclear Periphery | -1.0 | -1.2 | 1.5 | 0.4 | – | 0.8 | 1.3 | 1.0 | 1.4 | 1.2 | -0.7 | -0.6 | 0 | 0 | – |
Nucleolus | 0 | 0.9 | 3.0 | 2.7 | – | 2.9 | 2.8 | 2.5 | 1.0 | 0 | 0 | 0.5 | 0 | 0 | – |
Peroxisomes | 1.0 | 1.4 | 0 | 0.4 | – | 0 | -0.3 | -2.1 | -1.9 | -0.8 | -1.0 | -2.0 | 0 | 0 | – |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Vacuole | -0.1 | 3.9 | -0.4 | 0.5 | – | -0.6 | -1.2 | -2.3 | -0.9 | 0.4 | -0.3 | 0.3 | 2.1 | 0 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.4449 | 5.7403 | 5.0795 | 4.2724 | 3.6913 | 5.3454 | 7.3334 | 8.5148 | 8.1903 | 8.0658 | 7.1418 | 8.4803 | 9.2884 | 10.6745 | 10.2606 | 9.4095 | 9.7382 | 10.6671 |
Actin | 0.0672 | 0.002 | 0.0504 | 0.0019 | 0.006 | 0.0065 | 0.0571 | 0.0113 | 0.0156 | 0.0055 | 0.0105 | 0.0002 | 0.0398 | 0.0145 | 0.0091 | 0.0006 | 0.008 | 0.0004 |
Bud | 0.0007 | 0.0006 | 0.0002 | 0.0009 | 0.0001 | 0.0001 | 0.001 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0 | 0.0002 | 0.0024 | 0.0003 | 0.0002 | 0.0002 | 0 |
Bud Neck | 0.0072 | 0.0002 | 0.0002 | 0.0009 | 0 | 0.0001 | 0.0012 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0 |
Bud Periphery | 0.0011 | 0.0009 | 0.0003 | 0.0024 | 0.0003 | 0.0002 | 0.0022 | 0.0005 | 0.0009 | 0.0005 | 0.0004 | 0 | 0.0005 | 0.0027 | 0.0005 | 0.0007 | 0.0015 | 0.0002 |
Bud Site | 0.0029 | 0.0045 | 0.0006 | 0.0071 | 0.0002 | 0.0001 | 0.0086 | 0.0015 | 0.0021 | 0.0003 | 0.0009 | 0 | 0.0006 | 0.0131 | 0.0011 | 0.0003 | 0.0002 | 0 |
Cell Periphery | 0.0005 | 0.0004 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0009 | 0.001 | 0.0047 | 0.0002 | 0.0001 | 0 | 0.0011 | 0.0009 | 0.0003 | 0.0001 | 0.0003 | 0.0001 |
Cytoplasm | 0.0221 | 0.0003 | 0.0039 | 0.0001 | 0 | 0.0002 | 0.0051 | 0.0001 | 0.0008 | 0 | 0.0049 | 0 | 0.002 | 0.0006 | 0 | 0 | 0.0004 | 0 |
Cytoplasmic Foci | 0.0056 | 0.0016 | 0.0082 | 0.0009 | 0.005 | 0.0013 | 0.0075 | 0.0014 | 0.0046 | 0.0005 | 0.0099 | 0.0002 | 0.0177 | 0.0013 | 0.0018 | 0.0022 | 0.0326 | 0.0005 |
Eisosomes | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0014 | 0.0033 | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0.0006 | 0.0002 | 0.0011 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0102 | 0.0002 | 0.0006 | 0.0003 | 0.0001 | 0.0011 | 0.0014 | 0.0001 | 0.0006 | 0.0001 | 0.0025 | 0 | 0.0022 | 0.0002 | 0 | 0 | 0.0002 | 0.0002 |
Endosome | 0.0322 | 0.0057 | 0.0125 | 0.0068 | 0.0075 | 0.0105 | 0.0133 | 0.0053 | 0.0019 | 0.0012 | 0.0152 | 0.0015 | 0.01 | 0.0049 | 0.0056 | 0.0047 | 0.0014 | 0.0079 |
Golgi | 0.0089 | 0.0071 | 0.0293 | 0.003 | 0.0152 | 0.0244 | 0.0102 | 0.0098 | 0.0113 | 0.002 | 0.0039 | 0.0032 | 0.0078 | 0.0089 | 0.0022 | 0.0051 | 0.0019 | 0.0052 |
Lipid Particles | 0.0046 | 0.0028 | 0.0043 | 0.0116 | 0.0739 | 0.0041 | 0.0196 | 0.0098 | 0.0095 | 0.0011 | 0.0052 | 0.0096 | 0.0224 | 0.0028 | 0.0029 | 0.0093 | 0.005 | 0.0017 |
Mitochondria | 0.7613 | 0.9585 | 0.857 | 0.9484 | 0.8597 | 0.9373 | 0.8214 | 0.9398 | 0.937 | 0.9844 | 0.8788 | 0.9821 | 0.8285 | 0.9185 | 0.9356 | 0.9581 | 0.8978 | 0.9694 |
None | 0.0118 | 0.0001 | 0.0015 | 0.0002 | 0.0003 | 0.0005 | 0.0055 | 0 | 0.0008 | 0.0001 | 0.0032 | 0 | 0.0063 | 0.0001 | 0 | 0 | 0.001 | 0 |
Nuclear Periphery | 0.0303 | 0.0002 | 0.0049 | 0.0002 | 0.0003 | 0.0075 | 0.0043 | 0.0001 | 0.0003 | 0.0001 | 0.0191 | 0 | 0.0049 | 0 | 0.0001 | 0 | 0.0005 | 0.0005 |
Nucleolus | 0.0008 | 0.0002 | 0.0001 | 0.0013 | 0.0002 | 0.0002 | 0.0021 | 0.0002 | 0.0012 | 0.0001 | 0.0004 | 0.0001 | 0.0005 | 0.0006 | 0.0003 | 0.0003 | 0.0001 | 0 |
Nucleus | 0.0099 | 0.0002 | 0.0063 | 0.0008 | 0.0001 | 0.0006 | 0.0061 | 0.0001 | 0.0003 | 0.0001 | 0.0053 | 0 | 0.0007 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Peroxisomes | 0.0043 | 0.0061 | 0.0081 | 0.0026 | 0.0263 | 0.0011 | 0.01 | 0.0047 | 0.0018 | 0.0019 | 0.0299 | 0.0007 | 0.016 | 0.0037 | 0.0152 | 0.0137 | 0.0118 | 0.001 |
Punctate Nuclear | 0.0052 | 0 | 0.0029 | 0.0001 | 0.0005 | 0.0006 | 0.0062 | 0 | 0.0009 | 0 | 0.0075 | 0 | 0.0041 | 0 | 0 | 0 | 0.032 | 0 |
Vacuole | 0.0063 | 0.0037 | 0.0022 | 0.007 | 0.0009 | 0.0006 | 0.005 | 0.0029 | 0.0015 | 0.0005 | 0.0007 | 0.0005 | 0.0126 | 0.0094 | 0.0068 | 0.0013 | 0.0008 | 0.0055 |
Vacuole Periphery | 0.0065 | 0.0048 | 0.006 | 0.0024 | 0.003 | 0.0025 | 0.01 | 0.0079 | 0.0027 | 0.0011 | 0.001 | 0.0017 | 0.0211 | 0.0145 | 0.0168 | 0.0029 | 0.004 | 0.0073 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 42.7244 | 53.1381 | 43.1274 | 56.3245 | 46.9645 | 51.9835 | 58.7649 | 67.9926 | 58.2653 | 65.4768 |
Translational Efficiency | 1.8788 | 1.2129 | 1.6749 | 1.0448 | 1.535 | 2.1301 | 1.8046 | 1.5147 | 1.9351 | 1.6077 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |