Standard name
Description Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 – 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0.06 – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0.09 0 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0 0.05 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0.27 0.09 0.19 0 0.05 – 0 0 0 0 0.08 0.1 0.2 0.39 0.59 – 0.06 0.06 0 0.07 0.05 0.07
Mitochondria 0.9 0.88 0.81 0.86 0.63 0.83 0.71 – 0.88 0.8 0.89 0.85 0.9 0.91 0.86 0.11 0.06 – 0.81 0.79 0.88 0.76 0.77 0.69
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0
Nuclear Periphery 0.09 0 0 0 0 0.07 0 – 0.05 0.06 0.06 0.06 0.06 0 0 0 0.06 – 0 0 0 0 0 0
Nucleolus 0.08 0 0 0 0.05 0.1 0.09 – 0.1 0.09 0.09 0.05 0 0 0 0.07 0.06 – 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0.07 0.08 0 0.05 – 0 0 0 0 0 0 0 0.05 0.06 – 0 0 0 0.06 0.07 0.06
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.19 0.11 0.1 0.26 0.1 0.12 – 0.09 0.08 0.05 0.08 0.12 0.1 0.12 0.21 0.24 – 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 4 3 1 0 0 – 0 0 0 0 0 2 0 2 1 – 2 2 0 3 13 16
Bud 0 3 1 4 2 10 7 – 11 7 3 7 0 2 1 0 0 – 0 1 0 0 2 4
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 1
Bud Site 0 1 0 0 0 0 2 – 1 3 2 0 0 0 0 0 0 – – – – – – –
Cell Periphery 1 7 4 11 8 1 6 – 0 5 6 3 2 9 4 2 1 – 0 0 0 0 0 3
Cytoplasm 2 0 3 7 7 13 17 – 6 12 6 13 1 8 9 5 0 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 5 0 1 0 2 1 – 1 3 1 2 0 0 1 3 0 – 0 0 0 0 0 0
Endosome 3 2 5 4 17 4 16 – 3 11 1 3 2 4 6 0 0 – 4 4 2 0 5 4
Golgi 3 5 53 18 44 5 16 – 5 8 5 2 12 28 54 22 10 – 11 13 6 12 18 25
Mitochondria 168 168 159 175 144 300 253 – 237 316 257 250 129 258 236 6 1 – 158 167 190 133 282 238
Nucleus 1 1 0 0 2 7 2 – 3 4 1 0 0 1 0 3 0 – 0 0 0 0 0 1
Nuclear Periphery 17 5 7 4 4 24 15 – 14 24 16 19 9 7 7 1 1 – 1 1 0 2 3 6
Nucleolus 15 6 6 3 11 36 32 – 27 37 25 14 4 6 11 4 1 – 2 2 1 2 6 5
Peroxisomes 4 4 9 14 18 1 19 – 1 16 4 5 4 8 4 3 1 – 6 3 9 9 23 19
SpindlePole 1 0 1 0 6 1 6 – 1 8 2 1 1 1 1 0 0 – 1 1 1 1 1 2
Vac/Vac Membrane 18 36 21 21 58 35 43 – 24 30 15 24 17 28 32 12 4 – 2 10 2 3 5 10
Unique Cell Count 187 191 197 204 227 361 354 269 393 289 293 143 282 276 56 17 196 212 216 176 367 344
Labelled Cell Count 233 243 273 265 322 439 435 334 484 344 343 181 362 366 63 20 196 212 216 176 367 344


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.9 10.9 11.1 10.9 13.1 9.3 12.1 – 10.4 11.6 11.2 11.2 12.3 12.7 13.2 22.6 22.2 – 11.0 12.2 12.5
Std Deviation (1e-4) 2.7 2.9 2.3 2.3 7.6 2.5 3.2 – 4.1 5.2 3.5 5.5 2.6 3.3 3.4 7.5 6.1 – 3.2 3.1 3.0
Intensity Change (Log2) – – – -0.03 0.24 -0.25 0.13 – -0.09 0.07 0.02 0.01 0.15 0.19 0.26 1.03 1.0 – -0.01 0.14 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Bud 0 0 0 0 – 2.4 0 0 0 0 0 0 0 0 –
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Cell Periphery 1.8 0.9 0 0 – 0 0 0 0 0 0.8 0 0 0 –
Cytoplasm 0 0 1.4 2.0 – 0 1.1 0 1.8 0 0 0 0 0 –
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Endosome 0 2.3 0 1.2 – 0 0.2 0 0 0 0 0 0 0 –
Golgi -4.7 -1.8 -9.4 -7.6 – -8.1 -9.4 -8.4 -9.0 -4.3 -4.9 -1.9 1.8 2.8 –
Mitochondria 1.4 -3.9 0.7 -2.4 – 2.2 -0.1 2.5 1.3 2.4 3.5 1.4 -9.7 -6.8 –
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Nuclear Periphery -1.0 -1.2 1.5 0.4 – 0.8 1.3 1.0 1.4 1.2 -0.7 -0.6 0 0 –
Nucleolus 0 0.9 3.0 2.7 – 2.9 2.8 2.5 1.0 0 0 0.5 0 0 –
Peroxisomes 1.0 1.4 0 0.4 – 0 -0.3 -2.1 -1.9 -0.8 -1.0 -2.0 0 0 –
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 –
Vacuole -0.1 3.9 -0.4 0.5 – -0.6 -1.2 -2.3 -0.9 0.4 -0.3 0.3 2.1 0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.4449 5.7403 5.0795 4.2724 3.6913 5.3454 7.3334 8.5148 8.1903 8.0658 7.1418 8.4803 9.2884 10.6745 10.2606 9.4095 9.7382 10.6671
Actin 0.0672 0.002 0.0504 0.0019 0.006 0.0065 0.0571 0.0113 0.0156 0.0055 0.0105 0.0002 0.0398 0.0145 0.0091 0.0006 0.008 0.0004
Bud 0.0007 0.0006 0.0002 0.0009 0.0001 0.0001 0.001 0.0002 0.0003 0.0002 0.0003 0 0.0002 0.0024 0.0003 0.0002 0.0002 0
Bud Neck 0.0072 0.0002 0.0002 0.0009 0 0.0001 0.0012 0.0001 0.0004 0.0001 0.0002 0 0.0004 0.0004 0.0002 0.0001 0.0001 0
Bud Periphery 0.0011 0.0009 0.0003 0.0024 0.0003 0.0002 0.0022 0.0005 0.0009 0.0005 0.0004 0 0.0005 0.0027 0.0005 0.0007 0.0015 0.0002
Bud Site 0.0029 0.0045 0.0006 0.0071 0.0002 0.0001 0.0086 0.0015 0.0021 0.0003 0.0009 0 0.0006 0.0131 0.0011 0.0003 0.0002 0
Cell Periphery 0.0005 0.0004 0.0001 0.0008 0.0001 0.0001 0.0009 0.001 0.0047 0.0002 0.0001 0 0.0011 0.0009 0.0003 0.0001 0.0003 0.0001
Cytoplasm 0.0221 0.0003 0.0039 0.0001 0 0.0002 0.0051 0.0001 0.0008 0 0.0049 0 0.002 0.0006 0 0 0.0004 0
Cytoplasmic Foci 0.0056 0.0016 0.0082 0.0009 0.005 0.0013 0.0075 0.0014 0.0046 0.0005 0.0099 0.0002 0.0177 0.0013 0.0018 0.0022 0.0326 0.0005
Eisosomes 0.0005 0.0002 0.0003 0.0002 0.0002 0.0003 0.0014 0.0033 0.0007 0.0002 0.0002 0.0001 0.0006 0.0002 0.0011 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0102 0.0002 0.0006 0.0003 0.0001 0.0011 0.0014 0.0001 0.0006 0.0001 0.0025 0 0.0022 0.0002 0 0 0.0002 0.0002
Endosome 0.0322 0.0057 0.0125 0.0068 0.0075 0.0105 0.0133 0.0053 0.0019 0.0012 0.0152 0.0015 0.01 0.0049 0.0056 0.0047 0.0014 0.0079
Golgi 0.0089 0.0071 0.0293 0.003 0.0152 0.0244 0.0102 0.0098 0.0113 0.002 0.0039 0.0032 0.0078 0.0089 0.0022 0.0051 0.0019 0.0052
Lipid Particles 0.0046 0.0028 0.0043 0.0116 0.0739 0.0041 0.0196 0.0098 0.0095 0.0011 0.0052 0.0096 0.0224 0.0028 0.0029 0.0093 0.005 0.0017
Mitochondria 0.7613 0.9585 0.857 0.9484 0.8597 0.9373 0.8214 0.9398 0.937 0.9844 0.8788 0.9821 0.8285 0.9185 0.9356 0.9581 0.8978 0.9694
None 0.0118 0.0001 0.0015 0.0002 0.0003 0.0005 0.0055 0 0.0008 0.0001 0.0032 0 0.0063 0.0001 0 0 0.001 0
Nuclear Periphery 0.0303 0.0002 0.0049 0.0002 0.0003 0.0075 0.0043 0.0001 0.0003 0.0001 0.0191 0 0.0049 0 0.0001 0 0.0005 0.0005
Nucleolus 0.0008 0.0002 0.0001 0.0013 0.0002 0.0002 0.0021 0.0002 0.0012 0.0001 0.0004 0.0001 0.0005 0.0006 0.0003 0.0003 0.0001 0
Nucleus 0.0099 0.0002 0.0063 0.0008 0.0001 0.0006 0.0061 0.0001 0.0003 0.0001 0.0053 0 0.0007 0.0002 0.0001 0.0001 0.0001 0.0001
Peroxisomes 0.0043 0.0061 0.0081 0.0026 0.0263 0.0011 0.01 0.0047 0.0018 0.0019 0.0299 0.0007 0.016 0.0037 0.0152 0.0137 0.0118 0.001
Punctate Nuclear 0.0052 0 0.0029 0.0001 0.0005 0.0006 0.0062 0 0.0009 0 0.0075 0 0.0041 0 0 0 0.032 0
Vacuole 0.0063 0.0037 0.0022 0.007 0.0009 0.0006 0.005 0.0029 0.0015 0.0005 0.0007 0.0005 0.0126 0.0094 0.0068 0.0013 0.0008 0.0055
Vacuole Periphery 0.0065 0.0048 0.006 0.0024 0.003 0.0025 0.01 0.0079 0.0027 0.0011 0.001 0.0017 0.0211 0.0145 0.0168 0.0029 0.004 0.0073

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.7244 53.1381 43.1274 56.3245 46.9645 51.9835 58.7649 67.9926 58.2653 65.4768
Translational Efficiency 1.8788 1.2129 1.6749 1.0448 1.535 2.1301 1.8046 1.5147 1.9351 1.6077

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘