Standard name
Human Ortholog
Description Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.18 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0 0.1 0 0.05 0 0 0 0
Bud 0 0 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.07 0.06 0 0 0 0 0 0 0 0 0.07 0 0 0.11 0 0.07 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0.05 0.05 0.12 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.12 0 0 0 0 0 0 0
Endosome 0 0 0 0.05 0.05 0.08 0 0 0 0 0 0 0 0 0.05 0 0 0 0.08 0.07 0.09 0.07 0.07 0.08
Golgi 0.06 0.1 0.28 0.29 0.26 0 0 0 0 0 0 0 0.27 0.53 0.6 0.65 0.56 0.59 0.05 0.16 0.06 0.09 0.11 0.09
Mitochondria 0.89 0.94 0.59 0.84 0.68 0.73 0.85 0.77 0.91 0.89 0.93 0.86 0.73 0.48 0.3 0.05 0 0.14 0.72 0.59 0.75 0.68 0.62 0.64
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0.08 0.06 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.14 0.17 0.1 0.21 0.22 0.11 0.14 0.13 0.12 0.06 0.15 0.32 0.26 0.27 0.25 0.31 0.24 0 0.07 0 0.05 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 0 18 14 14 1 0 5 1 1 0 3 6 14 7 10 0 3 2 3 1 2 11 14
Bud 5 0 5 11 21 6 12 17 19 14 21 23 5 4 2 0 0 0 0 0 0 2 7 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 8
Bud Site 0 0 0 0 1 1 2 1 7 1 2 5 0 0 0 0 0 0
Cell Periphery 4 0 7 21 18 9 3 7 4 5 5 4 21 18 20 8 0 2 0 0 0 0 0 0
Cytoplasm 4 2 3 7 22 19 12 28 16 21 16 17 11 21 24 4 2 1 0 0 0 0 1 3
Endoplasmic Reticulum 4 3 8 3 11 3 3 6 3 6 8 4 2 1 11 5 2 1 1 0 0 1 5 2
Endosome 2 0 3 16 30 39 20 24 2 2 0 11 7 16 22 1 0 1 16 5 12 17 37 45
Golgi 11 8 28 101 150 16 3 9 2 1 1 4 88 233 276 49 9 17 11 14 8 23 57 53
Mitochondria 177 79 59 296 396 349 514 473 429 501 430 446 235 214 136 4 0 4 150 51 108 175 321 355
Nucleus 0 0 0 1 4 8 11 22 10 14 5 15 3 3 0 0 0 0 0 0 0 2 4 3
Nuclear Periphery 7 1 1 2 9 15 24 34 15 22 14 7 2 5 2 0 0 0 4 0 0 0 6 4
Nucleolus 16 0 0 4 10 16 46 21 16 9 12 9 3 5 4 6 1 1 1 0 0 1 2 4
Peroxisomes 0 0 1 5 5 1 2 1 2 0 1 0 9 8 13 0 0 0 5 2 5 9 15 20
SpindlePole 0 0 5 5 14 8 16 23 13 8 3 15 3 1 2 0 0 0 2 0 0 4 5 4
Vac/Vac Membrane 26 12 17 36 121 104 66 84 61 68 29 79 103 114 124 19 5 7 4 5 3 12 31 26
Unique Cell Count 200 84 100 352 583 475 608 612 472 566 463 516 323 442 461 75 16 29 209 87 144 258 518 560
Labelled Cell Count 259 105 155 522 826 595 734 755 600 673 547 642 498 657 643 106 19 37 209 87 144 258 518 560


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 8.5 7.6 8.0 8.3 6.9 6.0 6.5 5.4 5.6 4.9 5.6 10.5 11.1 10.6 14.6 15.0 15.0 7.9 8.2 8.1
Std Deviation (1e-4) 1.7 1.4 1.2 1.5 1.6 1.6 1.4 1.4 1.3 1.4 1.3 1.7 2.6 2.5 2.2 3.6 3.1 4.4 1.5 1.5 1.8
Intensity Change (Log2) 0.07 0.13 -0.14 -0.34 -0.24 -0.5 -0.44 -0.63 -0.45 0.46 0.54 0.47 0.94 0.98 0.98 0.05 0.1 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -4.8 0 0 0 0 0 0 0 0 -6.1 -5.7 0 -0.8 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.4 0 0 0 0 0 0 0 0 -0.2 0 0 0.9 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 -0.3 0 0
Endosome 0 0 1.8 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.1 -0.5 -8.4 0 -11.1 -11.2 0 -11.4 -11.2 -0.1 4.5 5.8 4.9 2.3 3.0
Mitochondria 5.4 1.7 2.9 6.0 3.9 8.2 7.4 9.1 6.5 2.6 -1.9 -5.6 -7.3 -4.4 -4.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2.8 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.9 0.9 1.1 -1.8 -0.9 -1.1 -1.4 -3.6 -0.4 2.9 1.9 2.1 1.3 0 0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.9126 13.0071 11.926 11.2337 8.981 12.2443 9.6629 11.1583 10.18 9.1566 7.5951 9.7602 11.136 11.1304 9.9855 9.3283 9.3003 9.2895
Actin 0.0073 0.0074 0.0076 0.0393 0.0127 0.0011 0.0156 0.0072 0.0057 0.0119 0.0018 0.0014 0.014 0.0024 0.004 0.0003 0.001 0.0047
Bud 0.0004 0.0008 0.0007 0.0002 0.0004 0 0.0007 0.0018 0.0009 0.0014 0.0002 0.0001 0.0003 0.0004 0.0004 0.0001 0.0001 0.0003
Bud Neck 0.0002 0.0001 0.0004 0.0003 0.0001 0 0.0027 0.0006 0.0003 0.0001 0.0001 0 0.0002 0.0002 0.0001 0.0001 0 0.0002
Bud Periphery 0.0015 0.001 0.0009 0.0003 0.0008 0.0001 0.0015 0.0035 0.0014 0.0073 0.0003 0.0002 0.0008 0.0008 0.0007 0.0004 0.0002 0.0008
Bud Site 0.0005 0.0032 0.0012 0.0007 0.0007 0 0.0036 0.0112 0.0065 0.0004 0.0004 0 0.0006 0.0014 0.0006 0.0001 0 0.001
Cell Periphery 0.0003 0.0003 0.0004 0.0001 0.0001 0.0001 0.0005 0.0013 0.0003 0.0007 0.0001 0.0001 0.0003 0.0005 0.0002 0.0004 0.0001 0.0002
Cytoplasm 0.0001 0.0001 0.0003 0.0003 0.0015 0 0.0006 0.0003 0.0008 0.0002 0.0004 0 0.0001 0.0001 0.0001 0 0 0.0001
Cytoplasmic Foci 0.0045 0.0037 0.0124 0.0087 0.0136 0.0013 0.0284 0.006 0.011 0.0047 0.0103 0.0007 0.0059 0.007 0.0021 0.0001 0.0002 0.0018
Eisosomes 0.0003 0.0003 0.0002 0.0005 0.0003 0.0001 0.0003 0.0004 0.0002 0.0001 0.0001 0.0001 0.0003 0.0002 0.0002 0.0001 0.0001 0.0003
Endoplasmic Reticulum 0.0003 0.0002 0.0012 0.0005 0.0007 0.0002 0.0005 0.0002 0.0002 0.0001 0.0004 0.0002 0.0002 0.0002 0.0002 0 0 0.0005
Endosome 0.0163 0.0166 0.0215 0.0164 0.0264 0.011 0.0208 0.0217 0.0174 0.0172 0.0425 0.0114 0.0136 0.0188 0.0112 0.0018 0.0015 0.0169
Golgi 0.0277 0.0443 0.0378 0.0262 0.0408 0.0299 0.0331 0.0385 0.0345 0.0424 0.0284 0.0207 0.0267 0.0297 0.0273 0.0023 0.003 0.0445
Lipid Particles 0.0164 0.0018 0.0187 0.0243 0.0059 0.0034 0.0331 0.0098 0.008 0.0031 0.01 0.0031 0.0258 0.0027 0.0017 0.0007 0.0005 0.0036
Mitochondria 0.896 0.9066 0.8644 0.8681 0.8748 0.9393 0.8073 0.8607 0.8897 0.8417 0.8892 0.9393 0.8897 0.907 0.942 0.974 0.9904 0.9164
None 0.0001 0 0.0004 0.0006 0.0007 0 0.0009 0.0001 0.0003 0.0001 0.0002 0 0.0001 0 0 0 0 0.0001
Nuclear Periphery 0.0007 0 0.0004 0.001 0.0026 0.0004 0.0013 0.0001 0.0002 0.0001 0.0004 0.0004 0.0002 0.0002 0.0002 0 0 0.0004
Nucleolus 0.0003 0.0002 0.0004 0.0002 0.0002 0 0.001 0.0028 0.0009 0.0002 0.0004 0 0.0006 0.0004 0.0001 0.0002 0 0.0012
Nucleus 0.0002 0.0001 0.0002 0.0002 0.0006 0 0.0004 0.0004 0.0003 0.0001 0.0003 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0005
Peroxisomes 0.0062 0.0078 0.0142 0.007 0.0056 0.0046 0.0209 0.0107 0.0144 0.0332 0.0028 0.0004 0.0096 0.0057 0.0035 0.0008 0.0006 0.0018
Punctate Nuclear 0.0002 0 0.0018 0.0027 0.0019 0 0.005 0.0001 0.0001 0.0001 0.0003 0 0.0007 0 0 0 0 0.0001
Vacuole 0.0051 0.0023 0.0056 0.0007 0.0016 0.001 0.0057 0.0101 0.003 0.0163 0.0076 0.0053 0.0038 0.0088 0.0019 0.0084 0.0004 0.0014
Vacuole Periphery 0.0152 0.0031 0.0093 0.0017 0.008 0.0072 0.0161 0.0123 0.0039 0.0183 0.004 0.0163 0.0064 0.0133 0.0034 0.0101 0.0019 0.0031

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 91.9504 77.2026 120.641 126.3785 171.2519 93.4227 89.3092 101.0309 123.9166 84.8607
Translational Efficiency 0.9753 1.0999 1.1391 1.4912 1.0171 1.0087 1.0182 1.1088 0.9841 1.2069

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
35 982 608 147 1464 1457 162 985 1499 2439 770 1132

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 940.07 1035.58 1556.94 1557.13 919.10 1133.47 1514.27 1247.18 919.59 1094.06 1547.96 1287.43
Standard Deviation 250.21 267.45 304.93 304.26 153.39 210.79 306.63 249.18 156.36 240.10 305.78 277.32
Intensity Change Log 2 0.139599 0.727873 0.728049 0.302452 0.720329 0.440376 0.222405 0.724148 0.592987

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.004349 0.006818 0.004728 0.010776 0.000996 0.001751 0.006618 0.016031 0.001075 0.003791 0.005126 0.015349
Bud Neck 0.002787 0.005092 0.003556 0.009898 0.001910 0.001215 0.016662 0.004678 0.001931 0.002776 0.006314 0.005356
Bud Site 0.029922 0.015122 0.008319 0.042945 0.006680 0.007998 0.041905 0.151125* 0.007223 0.010866 0.015385 0.137077*
Cell Periphery 0.000477 0.001225 0.000321 0.001157 0.000356 0.000268 0.001106 0.005799 0.000359 0.000654 0.000486 0.005196
Cytoplasm 0.014408 0.001685 0.001064 0.004287 0.001014 0.001076 0.003003 0.029032 0.001327 0.001321 0.001472 0.025818
Cytoplasmic Foci 0.040430 0.019168 0.025092 0.051505 0.023069 0.023488 0.090085 0.048763 0.023474 0.021748 0.038766 0.049119
Eisosomes 0.000089 0.000191 0.000147 0.000160 0.000042 0.000044 0.000144 0.000149 0.000043 0.000103 0.000146 0.000151
Endoplasmic Reticulum 0.009488 0.000753 0.000328 0.001156 0.000436 0.000402 0.001477 0.001986 0.000648 0.000543 0.000569 0.001878
Endosome 0.058955 0.036172 0.019990 0.060169 0.045621 0.038963 0.060249 0.059676 0.045933 0.037839 0.028460 0.059740
Golgi 0.098285 0.050081 0.085203 0.171905* 0.053704 0.063031 0.112343* 0.213489* 0.054745 0.057817 0.090913 0.208089*
Lipid Particles 0.004359 0.006312 0.002426 0.003849 0.001926 0.001455 0.003723 0.000368 0.001983 0.003411 0.002699 0.000820
Mitochondria 0.478706* 0.793652* 0.717576* 0.423368* 0.799828* 0.793674* 0.400154* 0.309446* 0.792330* 0.793665* 0.650794* 0.324239*
Mitotic Spindle 0.013876 0.004509 0.016288 0.043381 0.007955 0.007009 0.090328 0.042383 0.008093 0.006003 0.031865 0.042513
None 0.001944 0.001249 0.001379 0.001306 0.000321 0.000450 0.000775 0.000648 0.000359 0.000772 0.001252 0.000733
Nuclear Periphery 0.002058 0.000223 0.003138 0.002393 0.000269 0.000367 0.004485 0.000261 0.000311 0.000309 0.003421 0.000537
Nuclear Periphery Foci 0.040263 0.000590 0.001292 0.004037 0.000366 0.000317 0.004755 0.000605 0.001298 0.000427 0.002020 0.001051
Nucleolus 0.015209 0.001202 0.000840 0.000482 0.000421 0.000178 0.002924 0.000260 0.000766 0.000590 0.001278 0.000288
Nucleus 0.068232 0.000854 0.000224 0.000290 0.000387 0.000172 0.000966 0.000495 0.001971 0.000447 0.000380 0.000468
Peroxisomes 0.026120 0.016555 0.006247 0.020355 0.011583 0.006351 0.052024 0.013049 0.011923 0.010459 0.015878 0.013998
Vacuole 0.058187 0.021432 0.035729 0.068605 0.016671 0.022963 0.057742 0.056824 0.017640 0.022347 0.040360 0.058354
Vacuole Periphery 0.031855 0.017116 0.066114 0.077975 0.026445 0.028827 0.048530 0.044934 0.026571 0.024112 0.062414 0.049224

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.96 -0.16 -1.50 -1.03 -1.62 -1.26 -3.46 -7.47 -6.83 -3.59 -4.31 -5.05 -7.86 -6.04 -5.15
Bud Neck -1.44 -0.38 -3.56 -2.72 -3.71 1.55 -6.19 -5.16 -8.05 4.98 -1.59 -5.44 -6.28 -5.06 1.14
Bud Site 0.67 0.99 -0.65 -3.55 -4.45 -0.82 -4.07 -19.85 -19.52 -9.36 -2.38 -3.46 -19.90 -19.28 -17.73
Cell Periphery -3.55 1.46 -2.77 0.25 -3.74 1.36 -1.30 -4.01 -4.08 -3.18 -3.24 -0.97 -4.09 -3.84 -3.97
Cytoplasm 2.71 2.83 2.17 -2.23 -2.86 -0.27 -4.68 -12.92 -12.87 -11.81 0.20 -0.50 -12.78 -12.80 -12.73
Cytoplasmic Foci 1.77 1.22 -0.92 -4.51 -3.51 -0.19 -6.67 -9.23 -8.75 4.07 0.89 -4.43 -9.58 -10.77 -2.60
Eisosomes -4.03 -2.01 -2.55 1.17 -0.44 -0.94 -4.48 -9.10 -8.83 -0.11 -8.20 -5.89 -10.12 -3.68 -0.14
Endoplasmic Reticulum 1.80 1.88 1.72 -1.80 -4.05 0.39 -2.30 -7.32 -7.10 -0.84 0.80 0.45 -5.44 -6.92 -6.13
Endosome 1.48 2.62 -0.18 -3.30 -5.65 1.86 -2.61 -3.62 -5.85 0.20 2.52 5.19 -3.73 -7.22 -9.82
Golgi 1.60 0.35 -2.25 -6.16 -4.19 -1.97 -4.25 -18.48 -17.11 -6.48 -0.77 -5.16 -18.94 -19.04 -11.99
Lipid Particles -0.78 1.17 0.24 0.98 -0.71 0.73 -2.13 2.71 3.48 5.11 -1.68 -1.19 1.87 3.68 4.98
Mitochondria -4.64 -3.54 0.54 11.34 8.70 0.57 13.80 37.11* 36.66* 2.99 -0.16 9.53 36.36* 39.47* 19.97*
Mitotic Spindle 1.54 -0.45 -2.70 -4.08 -2.74 0.68 -5.29 -10.17 -10.52 2.94 1.72 -5.68 -10.68 -11.63 -2.26
None 1.01 0.91 1.00 -0.15 0.45 -4.32 -5.96 -6.18 -3.47 1.39 -3.51 -13.99 -6.98 0.34 6.76
Nuclear Periphery 1.76 -0.73 -0.19 -2.02 0.53 -1.01 -1.82 0.01 0.61 1.82 0.04 -3.52 -1.22 -1.23 3.19
Nuclear Periphery Foci 1.88 1.85 1.72 -1.61 -1.27 0.76 -2.59 -0.78 -0.93 2.39 1.69 -0.97 0.40 -1.58 1.57
Nucleolus 2.22 2.28 2.33 2.32 1.43 0.90 -2.18 0.54 -0.65 2.36 0.53 -1.18 1.52 1.86 3.08
Nucleus 2.75 2.78 2.78 1.88 -0.78 1.27 -1.45 -0.73 -2.00 1.04 2.33 2.41 2.19 -0.37 -0.61
Peroxisomes 0.76 1.67 0.39 -0.65 -2.44 2.89 -4.58 -0.71 -3.87 4.42 0.80 -1.50 -1.02 -2.02 0.75
Vacuole 1.96 1.37 0.03 -5.58 -3.75 -3.06 -5.77 -10.48 -8.82 -0.06 -2.30 -6.86 -11.23 -10.28 -4.33
Vacuole Periphery 1.23 -2.85 -3.24 -6.59 -1.12 -0.90 -3.46 -5.21 -4.52 0.53 1.08 -7.75 -6.60 -7.96 2.62