Standard name
Human Ortholog
Description Negative regulatory subunit of protein phosphatase 1 (Ppz1p); involved in coenzyme A biosynthesis; subunit of phosphopantothenoylcysteine decarboxylase (PPCDC: Cab3p, Sis2p, Vhs3p) complex and the CoA-Synthesizing Protein Complex (CoA-SPC: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p); SIS2 has a paralog, VHS3, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.05 0.05 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.4 0.42 0.41 0.56 0.49 0.31 0.22 0.15 0.15 0.19 0.22 0.27 0.43 0.45 0.51 0.48 0.32 0.44 0.09 0 0.08 0.13 0.1 0.14
Cytoplasm 0.89 0.89 0.82 0.82 0.66 0.72 0.72 0.79 0.73 0.75 0.74 0.65 0.94 0.91 0.91 0.84 0.86 0.84 0.69 0.84 0.7 0.52 0.69 0.65
Endoplasmic Reticulum 0.08 0.08 0.1 0.06 0.12 0.14 0.19 0.1 0.14 0.11 0.1 0.16 0 0 0.05 0 0 0 0.17 0.07 0.12 0.21 0.11 0.11
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.05 0.07 0.07 0.12 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 3 0 0 4 15 16 18 14 19 13 32 0 3 2 0 1 2 5 3 6 1 3 9
Bud 2 4 1 0 1 7 11 16 6 10 14 5 2 0 0 1 1 1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 4 6 1 5 5
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 125 124 72 134 134 112 93 73 42 70 82 114 97 125 148 145 80 137 29 8 15 7 14 34
Cytoplasm 280 264 144 196 181 259 306 391 210 277 278 279 212 252 264 255 216 262 219 262 130 32 97 155
Endoplasmic Reticulum 25 23 18 14 34 50 81 49 40 39 39 69 6 12 15 6 3 7 53 20 22 12 15 26
Endosome 0 0 0 0 0 0 0 0 1 0 1 0 0 0 1 0 0 0 0 1 0 0 0 1
Golgi 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3 16 23 20 27 45 35 0 0 0 0 0 1 0 2 0 0 0 1
Nucleus 0 1 3 1 3 1 2 2 1 1 1 3 0 1 0 2 1 6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 1 0 0 4 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 2 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 2 1 0 9 4 4 4 2 0 0 1 4 3 0 0 3 1 1 1 2
Unique Cell Count 314 297 175 238 275 361 425 497 289 368 378 426 225 276 291 303 250 311 318 311 186 62 140 239
Labelled Cell Count 435 420 238 346 360 448 526 587 338 448 478 539 317 393 431 414 308 416 318 311 186 62 140 239


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.7 13.8 13.8 13.1 12.3 9.7 9.4 8.8 7.8 8.1 7.5 7.8 14.0 13.7 14.1 17.8 17.6 17.7 11.2 14.1 14.6
Std Deviation (1e-4) 1.6 2.1 1.7 1.5 1.5 1.3 1.5 1.4 1.5 1.2 1.3 1.2 1.9 2.3 2.1 3.3 3.0 3.4 1.2 2.4 2.6
Intensity Change (Log2) -0.07 -0.17 -0.51 -0.56 -0.65 -0.82 -0.78 -0.88 -0.83 0.02 -0.01 0.03 0.37 0.35 0.35 -0.3 0.03 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 3.0 3.1 0 3.7 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3.0 1.6 -2.3 -4.8 -7.3 -6.5 -5.5 -4.7 -3.5 0.4 0.9 2.0 1.4 -1.9 0.6
Cytoplasm 0 -3.8 -2.6 -2.6 -1.0 -2.4 -1.8 -2.2 -4.1 3.8 2.9 2.7 0.5 1.2 0.6
Endoplasmic Reticulum -1.7 0.7 1.2 2.6 -0.2 1.1 0.1 0 1.9 -3.2 -2.5 -2.1 -4.0 -4.3 -3.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 2.9 3.6 3.7 4.8 3.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.8381 11.7055 11.3665 10.6377 9.5598 10.9792 7.7789 9.7317 9.0945 8.6877 6.6481 8.8095 7.9991 10.7109 9.579 9.9698 8.0415 9.5299
Actin 0.0048 0.0078 0.0113 0.0067 0.009 0.0029 0.0276 0.0138 0.0232 0.0201 0.0163 0.0139 0.0247 0.0047 0.0173 0.0099 0.0198 0.0092
Bud 0.0027 0.0004 0.0056 0.001 0.0006 0.0005 0.0008 0.0004 0.0018 0.0013 0.0028 0.0006 0.005 0.0003 0.0078 0.0024 0.0009 0.0003
Bud Neck 0.0021 0.0015 0.006 0.0064 0.0214 0.0112 0.0047 0.0018 0.0052 0.0037 0.0037 0.0116 0.0045 0.0027 0.0056 0.0069 0.0066 0.0247
Bud Periphery 0.0052 0.0011 0.0091 0.0029 0.0021 0.002 0.0016 0.0007 0.0074 0.0048 0.0031 0.0021 0.0047 0.0006 0.0045 0.0134 0.0024 0.0008
Bud Site 0.0022 0.0063 0.0099 0.0012 0.0028 0.0006 0.0102 0.0172 0.0081 0.0014 0.0029 0.0007 0.0154 0.0143 0.0268 0.0023 0.002 0.0004
Cell Periphery 0.0475 0.0281 0.0224 0.0185 0.0227 0.0217 0.0425 0.022 0.0296 0.0121 0.0121 0.0171 0.0314 0.0166 0.0233 0.045 0.014 0.0094
Cytoplasm 0.865 0.9027 0.9024 0.9389 0.848 0.9093 0.7405 0.8891 0.8372 0.8782 0.6824 0.8857 0.6958 0.9381 0.8598 0.8597 0.7598 0.8972
Cytoplasmic Foci 0.0029 0.0028 0.0019 0.0021 0.0032 0.0058 0.0216 0.004 0.0064 0.0034 0.0138 0.01 0.0222 0.0017 0.0041 0.0059 0.0177 0.0039
Eisosomes 0.0003 0.0003 0.0001 0.0001 0.0003 0.0001 0.0004 0.0001 0.0003 0 0.0001 0.0003 0.0006 0 0.0003 0.0002 0.0002 0
Endoplasmic Reticulum 0.0462 0.03 0.0215 0.0146 0.0685 0.0343 0.0702 0.0402 0.0457 0.0076 0.1056 0.0295 0.0608 0.0154 0.0141 0.0149 0.0321 0.028
Endosome 0.0019 0.0017 0.0017 0.0009 0.0033 0.0018 0.0279 0.0013 0.005 0.0398 0.0787 0.0075 0.0327 0.0006 0.0036 0.0006 0.0381 0.0047
Golgi 0.0029 0.0028 0.0019 0.001 0.0044 0.0016 0.019 0.0023 0.0064 0.0036 0.0254 0.0078 0.0208 0.0009 0.004 0.001 0.0281 0.0059
Lipid Particles 0.0049 0.0068 0.0005 0.0009 0.0007 0.0026 0.0102 0.0035 0.0094 0.0086 0.0056 0.0027 0.0187 0.0013 0.0057 0.0012 0.0088 0.0022
Mitochondria 0.0059 0.0037 0.0002 0.0002 0.0088 0.0003 0.005 0.0001 0.0034 0.0015 0.0317 0.0021 0.0246 0 0.0112 0.0022 0.0562 0.0058
None 0.0015 0.0008 0.0009 0.0005 0.0016 0.0009 0.0016 0.0005 0.0006 0.0009 0.0007 0.0006 0.0033 0.0003 0.0008 0.0302 0.001 0.0003
Nuclear Periphery 0.0005 0.0005 0.0004 0.0007 0.0004 0.0003 0.0018 0.0005 0.001 0.0026 0.0017 0.0013 0.0118 0.0003 0.0006 0.0005 0.0023 0.0003
Nucleolus 0.0001 0 0.0001 0 0 0.0002 0.0001 0 0.0001 0.0002 0.0002 0 0.0006 0 0.0001 0.0001 0.0001 0
Nucleus 0.0004 0.0004 0.0007 0.0006 0.0004 0.001 0.0007 0.0004 0.0004 0.0007 0.0016 0.0004 0.002 0.0004 0.0008 0.0005 0.0008 0.0003
Peroxisomes 0.0008 0.0003 0.0001 0.0001 0.0002 0.0003 0.0069 0.0001 0.0033 0.0003 0.0023 0.0043 0.0081 0 0.0034 0.0002 0.004 0.0051
Punctate Nuclear 0.0001 0.0001 0.0001 0.0001 0.0001 0.0009 0.0009 0.0001 0.0005 0.0003 0.0005 0.0003 0.0061 0.0001 0.0006 0.0002 0.0014 0.0001
Vacuole 0.0023 0.0018 0.0029 0.0025 0.0013 0.0017 0.0053 0.0019 0.0049 0.0069 0.006 0.0012 0.0048 0.0016 0.0053 0.0028 0.0027 0.0011
Vacuole Periphery 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0005 0 0.0002 0.0018 0.0027 0.0002 0.0013 0 0.0003 0.0001 0.0007 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.2977 39.712 30.7681 37.3576 34.3042 46.3578 32.0336 37.0638 40.8783 34.4542
Translational Efficiency 1.2537 0.9858 1.4165 1.165 1.1125 0.9307 0.9643 0.91 0.8989 1.1091

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1136 1070 1105 831 1897 1607 176 558 3033 2677 1281 1389

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 925.84 1087.40 1455.01 1234.30 1205.51 1151.22 1396.30 1224.67 1100.76 1125.71 1446.94 1230.43
Standard Deviation 134.32 217.27 183.65 180.02 161.66 172.33 174.98 179.55 203.53 194.09 183.60 179.89
Intensity Change Log 2 0.232048 0.652194 0.414858 -0.066480 0.211965 0.022749 0.070842 0.419857 0.206287

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003460 0.005303 0.051551 0.055756 0.005199 0.007988 0.089088 0.068798 0.004548 0.006915 0.056708 0.060995
Bud Neck 0.006506 0.005556 0.021381 0.020801 0.004076 0.008078 0.020920 0.021611 0.004986 0.007070 0.021317 0.021127
Bud Site 0.092896 0.095196 0.149997* 0.136698* 0.049625 0.078121 0.173921* 0.141795* 0.065832 0.084946 0.153284* 0.138746*
Cell Periphery 0.027918 0.013950 0.108209* 0.099678 0.019484 0.008146 0.125109* 0.123709* 0.022643 0.010466 0.110531* 0.109332*
Cytoplasm 0.699542* 0.653942* 0.387367* 0.409697* 0.785879* 0.687620* 0.184393* 0.393154* 0.753542* 0.674158* 0.359480* 0.403051*
Cytoplasmic Foci 0.083502 0.110271 0.017271 0.059201 0.057512 0.102976 0.074226 0.048706 0.067247 0.105892 0.025096 0.054985
Eisosomes 0.000981 0.000304 0.001722 0.005223 0.000593 0.000707 0.005632 0.006915 0.000738 0.000546 0.002259 0.005902
Endoplasmic Reticulum 0.028789 0.020898 0.051656 0.009183 0.026750 0.011444 0.052159 0.008238 0.027514 0.015223 0.051725 0.008803
Endosome 0.006982 0.016751 0.005529 0.014989 0.003383 0.013044 0.027905 0.014576 0.004731 0.014526 0.008604 0.014823
Golgi 0.014212 0.016174 0.007451 0.006417 0.009182 0.014465 0.008542 0.007920 0.011066 0.015148 0.007601 0.007021
Lipid Particles 0.006207 0.015160 0.133424* 0.047624 0.011587 0.008689 0.060258 0.043220 0.009572 0.011276 0.123372* 0.045855
Mitochondria 0.004118 0.000473 0.000187 0.001385 0.000906 0.002635 0.000027 0.001774 0.002109 0.001771 0.000165 0.001542
Mitotic Spindle 0.000153 0.000328 0.000645 0.003647 0.000249 0.002028 0.000370 0.002352 0.000213 0.001349 0.000607 0.003127
None 0.000695 0.000563 0.000263 0.000305 0.000660 0.000261 0.000027 0.000092 0.000673 0.000382 0.000230 0.000219
Nuclear Periphery 0.000075 0.000084 0.000152 0.000209 0.000039 0.000027 0.000122 0.000167 0.000052 0.000050 0.000148 0.000192
Nuclear Periphery Foci 0.000681 0.002467 0.006184 0.002959 0.000815 0.000506 0.007600 0.002344 0.000765 0.001290 0.006378 0.002712
Nucleolus 0.000376 0.000264 0.000038 0.000070 0.000081 0.000187 0.000020 0.000040 0.000191 0.000218 0.000036 0.000058
Nucleus 0.003188 0.002428 0.006421 0.004691 0.003488 0.004716 0.002747 0.004288 0.003376 0.003802 0.005916 0.004529
Peroxisomes 0.006216 0.002745 0.002773 0.006169 0.003501 0.003900 0.002955 0.007480 0.004518 0.003439 0.002798 0.006695
Vacuole 0.008945 0.031598 0.046989 0.114086 0.015556 0.041743 0.163559 0.100393 0.013080 0.037688 0.063005 0.108585
Vacuole Periphery 0.004556 0.005544 0.000791 0.001212 0.001437 0.002719 0.000419 0.002429 0.002605 0.003848 0.000740 0.001701

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.22 -18.68 -13.67 -13.08 -0.62 -3.76 -7.75 -13.06 -12.43 1.67 -4.70 -19.36 -18.70 -17.80 -0.86
Bud Neck 1.67 -13.91 -9.10 -9.80 0.55 -6.34 -8.23 -9.95 -7.31 -0.12 -4.71 -17.78 -13.87 -11.63 0.42
Bud Site -0.43 -9.20 -6.11 -5.72 1.98 -7.77 -8.21 -13.02 -8.60 2.05 -6.15 -17.07 -14.89 -10.56 2.52
Cell Periphery 5.56 -16.42 -10.35 -12.87 1.40 8.61 -7.25 -12.22 -13.70 0.24 9.65 -19.90 -16.33 -18.99 0.52
Cytoplasm 4.24 27.61 24.91 19.83 -1.43 12.30 45.05 32.13 22.88 -12.32 12.28 43.41 42.38 31.01 -3.72
Cytoplasmic Foci -5.01 20.92 4.56 8.45 -11.41 -10.98 -2.09 1.65 9.46 2.77 -11.79 18.31 3.15 12.39 -9.70
Eisosomes 4.37 -4.00 -7.74 -9.40 -6.52 -1.55 -7.06 -8.75 -8.58 -1.15 2.66 -10.15 -11.95 -12.50 -8.04
Endoplasmic Reticulum 2.17 -4.62 6.27 4.45 10.15 5.89 -2.59 7.10 1.75 4.61 5.76 -5.90 9.34 4.19 11.36
Endosome -6.60 1.74 -5.46 0.75 -7.16 -10.10 -4.94 -5.70 -0.62 2.53 -11.96 -4.65 -8.88 -0.33 -4.72
Golgi -1.66 8.07 5.50 6.59 0.95 -4.95 0.45 0.99 3.83 0.38 -5.14 6.82 4.19 7.14 0.77
Lipid Particles -6.40 -24.70 -10.65 -7.97 13.30 3.01 -6.98 -7.72 -8.45 2.10 -2.10 -24.71 -12.67 -11.96 14.34
Mitochondria 5.17 5.59 2.68 -1.12 -1.49 -2.91 5.65 -0.94 0.89 -1.99 0.79 6.88 0.98 0.41 -2.31
Mitotic Spindle -3.24 -13.83 -2.84 -2.71 -2.47 -2.34 -1.04 -1.74 -0.13 -1.64 -2.47 -6.73 -3.30 -1.79 -2.88
None 0.63 2.28 1.61 1.32 -0.20 3.43 5.59 4.93 4.94 -3.72 2.64 4.29 3.26 1.49 0.15
Nuclear Periphery -0.16 -1.89 -1.28 -1.23 -0.58 1.12 -5.77 -1.89 -2.06 -0.63 0.09 -5.63 -2.13 -2.17 -0.67
Nuclear Periphery Foci -4.97 -19.21 -6.84 -1.36 7.30 3.55 -8.53 -6.36 -7.54 6.22 -3.45 -21.60 -8.94 -5.82 10.55
Nucleolus 0.58 1.84 1.66 1.22 -3.54 -1.06 2.42 1.62 1.51 -3.37 -0.10 2.17 1.85 1.84 -3.69
Nucleus 0.72 -3.07 -1.56 -4.28 2.96 -1.56 1.38 -1.44 0.20 -3.13 -0.64 -4.14 -2.37 -1.88 2.62
Peroxisomes 6.29 6.34 0.24 -7.25 -7.34 -1.26 1.65 -2.97 -2.67 -3.39 3.84 6.23 -3.31 -5.37 -6.53
Vacuole -11.47 -21.24 -20.29 -15.44 -12.70 -12.17 -10.78 -13.61 -9.35 3.69 -16.13 -18.71 -23.90 -17.36 -10.34
Vacuole Periphery -1.26 7.30 5.64 6.52 -1.31 -4.59 6.45 -0.81 0.32 -1.73 -3.53 8.45 1.79 3.93 -1.90