ORF
Human Ortholog
Description Protein of unknown function; similar to Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; YKR075C has a paralog, YOR062C, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.42 0.75 0.97 0.87 0.92 0.82 0.78 0.77 0.24 0.49 0.14 0.39 0.99 1.0 1.0 0.68 0.8 0.28 0.54 0.76 0.83 0.8 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0.08 0.12 0.1 0.14 0.63 0.34 0.57 0.51 0 0 0 0 0 0 0.06 0.09 0 0 0
Nucleus 0.4 0.09 0 0.06 0 0.07 0.1 0.06 0.37 0.24 0.53 0.2 0 0 0 0.1 0.08 0.45 0.15 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Vac/Vac Membrane 0.25 0.14 0 0.05 0 0.06 0.05 0.07 0.05 0 0 0.1 0 0 0 0.27 0.11 0.14 0.11 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 1 2 4 0 2 1
Bud 0 0 0 1 4 2 0 2 9 2 6 4 0 0 0 0 0 9 6 1 0 4 3
Bud Neck 0 0 4 1 11 6 10 12 2 9 4 2 0 2 1 1 2 1 0 1 0 2 1
Bud Site 0 0 0 0 0 0 0 0 2 0 6 0 0 0 0 0 0
Cell Periphery 0 1 2 0 1 1 1 0 0 0 0 1 1 0 3 1 1 0 0 0 0 0 0
Cytoplasm 142 174 296 195 227 327 265 320 99 169 67 159 326 368 400 176 164 115 142 228 184 257 219
Endoplasmic Reticulum 1 4 1 0 0 0 1 0 0 1 0 0 0 0 1 13 15 0 0 1 1 0 1
Endosome 1 2 2 1 0 1 2 1 0 1 1 1 0 0 0 3 0 3 6 5 3 5 4
Golgi 0 1 2 1 1 0 0 0 0 0 0 0 0 0 0 1 0 1 4 2 0 0 0
Mitochondria 13 12 0 9 20 49 33 57 255 119 271 206 0 0 0 4 5 5 14 27 3 13 7
Nucleus 138 20 2 14 4 28 34 25 148 83 254 81 5 0 0 27 17 185 40 3 8 2 1
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 1 1 3 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 1 4 9 12 0 6 7 12 1 0 0 0 1 23 10 4 4 7 4
Vac/Vac Membrane 84 33 3 11 6 25 18 30 22 14 11 42 1 2 0 69 23 59 29 5 8 10 3
Unique Cell Count 341 231 304 225 248 400 341 415 405 345 479 406 330 368 402 260 205 416 266 301 222 321 256
Labelled Cell Count 379 248 312 233 275 444 374 459 539 405 631 510 334 372 405 295 228 416 266 301 222 321 256


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.0 4.6 4.5 3.9 3.9 3.9 4.3 3.5 3.8 3.8 3.7 4.5 4.7 4.3 4.3 5.3 5.4 4.7 5.1 5.4
Std Deviation (1e-4) 0.7 1.2 1.1 1.4 1.0 1.1 1.2 1.2 1.0 1.0 0.8 0.7 0.6 0.9 0.8 1.3 1.4 1.3 1.2 1.4 1.6
Intensity Change (Log2) -0.05 -0.24 -0.24 -0.23 -0.1 -0.38 -0.29 -0.28 -0.32 -0.02 0.02 -0.1 -0.11 0.2 0.22 0.02 0.14 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 9.1 5.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0963 1.6635 2.2744 2.0249 1.7824 2.048 5.3488 3.6781 4.7863 4.2455 4.4515 3.96 1.739 1.3576 1.4989 2.1001 0.5361 1.4275
Actin 0.0386 0.0001 0.0071 0.0008 0.018 0.003 0.074 0.0034 0.0265 0.0001 0.0107 0.0041 0.063 0.0003 0.0165 0.0746 0.0002 0.0008
Bud 0.0007 0.0001 0.0014 0.0003 0.0001 0.0003 0.0039 0.0056 0.0013 0 0.0001 0.0008 0.0022 0.0001 0.0001 0.0001 0.0007 0.0001
Bud Neck 0.0085 0 0.008 0 0.0059 0.0003 0.0085 0.0001 0.0011 0 0 0.0002 0.0092 0 0.0048 0.0003 0 0.0001
Bud Periphery 0.0014 0.0001 0.0036 0.0005 0.0003 0.001 0.0074 0.0088 0.0032 0 0.0002 0.0029 0.0078 0.0001 0.0002 0.0001 0.001 0.0002
Bud Site 0.0113 0.0002 0.0058 0.0001 0.0003 0.0002 0.0425 0.0047 0.0181 0 0.0001 0.0001 0.0039 0.0005 0.0015 0.0005 0 0
Cell Periphery 0.001 0.0001 0.0002 0 0.0001 0.0001 0.0031 0.0005 0.0007 0 0 0.0001 0.0005 0.0001 0.0001 0 0 0
Cytoplasm 0.0312 0.0568 0.035 0.0438 0.0393 0.0747 0.0638 0.1004 0.0468 0.014 0.0281 0.0869 0.023 0.0306 0.0226 0.0303 0.0404 0.0516
Cytoplasmic Foci 0.0215 0.0015 0.0149 0.0067 0.0099 0.0075 0.0164 0.004 0.0107 0.0018 0.001 0.0048 0.0142 0.0021 0.0116 0.0019 0.0024 0.0042
Eisosomes 0.0007 0.0002 0.0003 0.0001 0.0003 0.0001 0.0021 0.0006 0.0014 0.0001 0.0001 0.0001 0.0007 0.0001 0.0002 0.0008 0.0001 0
Endoplasmic Reticulum 0.0018 0.0005 0.0012 0.0009 0.0017 0.0008 0.007 0.0009 0.0008 0.0002 0.0051 0.0015 0.0021 0.0006 0.0025 0.0003 0.0004 0.0002
Endosome 0.015 0.0001 0.0067 0.0009 0.0082 0.0058 0.0312 0.0004 0.0062 0.0001 0.0111 0.0005 0.0064 0.0004 0.0025 0.0004 0.0002 0.0002
Golgi 0.0069 0 0.003 0.0002 0.0059 0.0009 0.0109 0.0002 0.0045 0 0.0022 0.0001 0.0104 0.0001 0.0017 0.0011 0 0
Lipid Particles 0.008 0.0001 0.0121 0.0007 0.0095 0.0042 0.0066 0.0002 0.009 0.0001 0.0007 0.0003 0.0036 0.0003 0.0032 0.001 0.0002 0.0001
Mitochondria 0.003 0.0001 0.0067 0.0004 0.0019 0.0113 0.0135 0.0009 0.0173 0.0001 0.0049 0.0009 0.0035 0.0001 0.0006 0.0004 0.0009 0.0001
None 0.8176 0.9393 0.8684 0.9372 0.8819 0.8658 0.6627 0.8661 0.8443 0.9828 0.8815 0.8783 0.8195 0.9631 0.9063 0.8847 0.9497 0.9392
Nuclear Periphery 0.0055 0.0001 0.0048 0.0016 0.0008 0.0029 0.0117 0.0004 0.0006 0.0001 0.0477 0.0014 0.0075 0.0002 0.0007 0.0002 0.0009 0.0003
Nucleolus 0.0018 0.0001 0.0008 0.0002 0.0003 0.0005 0.0023 0.0002 0.0002 0.0001 0.0001 0.0004 0.0007 0.0001 0.0006 0 0.0002 0
Nucleus 0.0047 0.0002 0.0016 0.0005 0.0004 0.009 0.0102 0.0008 0.0011 0.0001 0.0021 0.0026 0.0131 0.0004 0.0119 0.0002 0.0011 0.0007
Peroxisomes 0.0083 0.0001 0.0139 0.0008 0.0134 0.0043 0.0092 0.0004 0.0045 0.0001 0.0002 0.0006 0.0035 0.0001 0.0111 0.0028 0.0003 0.0002
Punctate Nuclear 0.0096 0.0001 0.0034 0.0037 0.0013 0.0064 0.0056 0.0009 0.0009 0.0002 0.0011 0.0129 0.0037 0.0003 0.0008 0.0004 0.0004 0.0018
Vacuole 0.0022 0.0002 0.0008 0.0003 0.0004 0.0005 0.0055 0.0004 0.0004 0.0001 0.0005 0.0002 0.0009 0.0003 0.0004 0.0001 0.0005 0.0001
Vacuole Periphery 0.0005 0 0.0004 0.0002 0.0002 0.0003 0.0018 0.0001 0.0002 0 0.0025 0.0001 0.0007 0 0.0001 0 0.0003 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.9854 5.3375 0.7921 5.7934 4.6448 2.387 4.3174 7.6492 3.8027 5.206
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1613 839 2543 637 1749 1791 1241 321 3362 2630 3784 958

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 681.50 754.07 776.94 769.74 637.89 784.06 791.45 857.36 658.81 774.49 781.70 799.10
Standard Deviation 80.90 95.42 101.71 110.82 69.13 88.26 97.79 139.36 78.11 91.68 100.67 128.00
Intensity Change Log 2 0.145985 0.189090 0.175658 0.297656 0.311191 0.426593 0.221306 0.249413 0.302432

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000061 0.000422 0.000104 0.002537 0.000193 0.000328 0.000182 0.003781 0.000129 0.000358 0.000130 0.002954
Bud Neck 0.007014 0.025361 0.007775 0.010006 0.008037 0.035967 0.004672 0.008898 0.007546 0.032583 0.006757 0.009635
Bud Site 0.002787 0.012715 0.009324 0.046906 0.001885 0.012448 0.004572 0.077139 0.002318 0.012533 0.007766 0.057036
Cell Periphery 0.000082 0.000164 0.000228 0.000295 0.000132 0.000187 0.000088 0.000205 0.000108 0.000179 0.000182 0.000265
Cytoplasm 0.426305* 0.352263* 0.185167* 0.393083* 0.471351* 0.397638* 0.144565* 0.372233* 0.449739* 0.383163* 0.171852* 0.386096*
Cytoplasmic Foci 0.095908 0.144968 0.003526 0.039609 0.159211 0.196665* 0.002008 0.018503 0.128840 0.180173* 0.003029 0.032537
Eisosomes 0.000055 0.000102 0.000026 0.000073 0.000116 0.000161 0.000019 0.000052 0.000087 0.000142 0.000024 0.000066
Endoplasmic Reticulum 0.000712 0.001597 0.001210 0.001903 0.000394 0.000470 0.002625 0.002706 0.000547 0.000829 0.001674 0.002172
Endosome 0.004225 0.010888 0.000970 0.019553 0.003285 0.008501 0.000736 0.019439 0.003736 0.009263 0.000893 0.019515
Golgi 0.000560 0.003212 0.000078 0.007461 0.001211 0.002996 0.000094 0.005086 0.000899 0.003065 0.000083 0.006665
Lipid Particles 0.005270 0.006432 0.000406 0.004528 0.006729 0.006359 0.000531 0.006550 0.006029 0.006383 0.000447 0.005205
Mitochondria 0.003530 0.012330 0.000865 0.008194 0.003828 0.009166 0.000688 0.003531 0.003685 0.010176 0.000807 0.006632
Mitotic Spindle 0.000665 0.006687 0.002984 0.042864 0.000594 0.006080 0.001583 0.069045 0.000628 0.006273 0.002525 0.051636
None 0.005327 0.010960 0.009343 0.007623 0.004392 0.004224 0.005308 0.009577 0.004840 0.006373 0.008020 0.008278
Nuclear Periphery 0.000838 0.000995 0.001251 0.001769 0.000495 0.000355 0.001574 0.001432 0.000660 0.000559 0.001357 0.001656
Nuclear Periphery Foci 0.000283 0.000853 0.000092 0.002043 0.000176 0.000146 0.000310 0.001519 0.000228 0.000372 0.000163 0.001867
Nucleolus 0.001678 0.004187 0.001081 0.002003 0.000987 0.001538 0.000408 0.000484 0.001319 0.002383 0.000860 0.001494
Nucleus 0.419749* 0.354218* 0.766103* 0.236617* 0.315531* 0.270383* 0.815815* 0.242055* 0.365532* 0.297127* 0.782406* 0.238439*
Peroxisomes 0.002502 0.017278 0.000116 0.005805 0.003696 0.015638 0.000152 0.001909 0.003123 0.016161 0.000128 0.004500
Vacuole 0.022042 0.032685 0.009051 0.155650 0.017387 0.030071 0.013949 0.149929 0.019621 0.030905 0.010657 0.153733
Vacuole Periphery 0.000406 0.001685 0.000299 0.011478 0.000369 0.000679 0.000121 0.005927 0.000387 0.001000 0.000240 0.009618

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.61 -5.91 -2.00 -1.24 -1.86 -1.01 -0.57 -2.13 -1.96 -2.06 -2.98 -0.71 -2.78 -2.23 -2.70
Bud Neck -6.38 -1.03 0.27 6.33 1.15 -11.35 1.75 1.11 11.71 -0.90 -13.11 0.21 0.89 13.08 0.61
Bud Site -4.50 -4.53 -5.47 -2.64 -3.73 -7.50 -1.98 -4.58 -2.95 -4.21 -8.51 -5.28 -7.20 -4.04 -5.55
Cell Periphery -7.31 -8.52 -4.13 -0.38 0.99 -4.19 4.32 -0.66 3.26 -4.59 -8.04 -5.29 -3.48 0.99 -0.01
Cytoplasm 6.41 31.59 7.77 1.81 -12.97 8.47 44.33 13.49 8.67 -8.74 9.56 52.28 14.69 7.72 -15.52
Cytoplasmic Foci -6.50 24.57 16.49 17.86 -9.40 -5.61 36.21 28.91 32.44 -5.83 -10.21 42.79 31.73 36.49 -11.02
Eisosomes -6.05 9.89 -6.01 2.74 -16.24 -4.82 12.80 4.18 9.95 -11.43 -8.84 15.63 1.12 10.68 -19.87
Endoplasmic Reticulum -5.12 -8.26 -8.71 -4.56 -4.52 -0.89 -17.15 -9.35 -9.15 -2.37 -3.30 -19.10 -12.97 -11.60 -4.66
Endosome -4.78 5.22 -2.59 1.23 -4.90 -7.17 8.25 -1.77 0.79 -3.01 -8.05 7.85 -3.34 1.00 -5.75
Golgi -4.57 4.24 -1.95 0.48 -2.43 -3.48 5.14 -0.11 1.95 -1.67 -5.61 6.51 -1.70 1.06 -2.85
Lipid Particles -1.36 9.03 2.41 3.28 -2.34 0.53 11.92 2.65 2.43 -2.02 -0.69 14.97 3.80 4.23 -3.07
Mitochondria -4.23 3.58 0.33 4.21 -2.24 -4.37 4.48 3.27 7.22 -1.41 -6.15 5.64 1.40 6.68 -2.46
Mitotic Spindle -3.53 -3.79 -5.61 -3.75 -4.98 -3.93 -2.64 -4.53 -3.75 -4.07 -5.20 -4.64 -7.19 -5.46 -6.42
None -2.69 -3.30 -0.10 2.55 2.58 0.09 -0.44 -1.57 -1.59 -1.43 -1.81 -3.39 -1.24 0.72 1.53
Nuclear Periphery -0.85 -8.34 -6.15 -4.94 -3.17 3.44 -18.24 -9.99 -10.61 -2.51 1.59 -19.23 -10.49 -10.93 -4.01
Nuclear Periphery Foci -2.54 4.62 -3.45 -1.57 -3.95 1.26 -6.25 -3.89 -3.94 -3.40 -1.94 -0.07 -5.22 -4.63 -5.22
Nucleolus -3.81 2.59 1.22 4.27 -0.41 -2.27 4.09 3.42 5.18 -1.44 -3.88 3.42 1.47 4.32 -0.50
Nucleus 5.20 -41.31 12.60 6.26 45.25 5.57 -58.70 0.76 -2.02 30.45 9.79 -69.96 9.24 2.08 54.35
Peroxisomes -6.23 6.99 0.57 6.22 -2.84 -10.65 9.72 7.06 13.85 -3.54 -12.15 11.95 2.77 12.69 -3.30
Vacuole -4.43 5.64 -22.88 -21.65 -23.70 -8.15 -5.98 -17.00 -16.23 -15.94 -8.31 -0.17 -28.65 -27.38 -28.57
Vacuole Periphery -5.34 1.96 -2.86 -1.84 -2.97 -4.22 5.39 -1.27 -0.93 -1.55 -6.70 3.55 -3.12 -2.44 -3.31